Job ID = 2589909 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 4,536,608 reads read : 4,536,608 reads written : 4,536,608 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR947550.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:55 4536608 reads; of these: 4536608 (100.00%) were unpaired; of these: 35028 (0.77%) aligned 0 times 3772680 (83.16%) aligned exactly 1 time 728900 (16.07%) aligned >1 times 99.23% overall alignment rate Time searching: 00:00:55 Overall time: 00:00:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 286842 / 4501580 = 0.0637 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 18:46:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:46:05: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:46:05: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:46:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:46:06: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:46:06: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:46:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:46:07: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:46:07: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:46:13: 1000000 INFO @ Mon, 12 Aug 2019 18:46:14: 1000000 INFO @ Mon, 12 Aug 2019 18:46:14: 1000000 INFO @ Mon, 12 Aug 2019 18:46:19: 2000000 INFO @ Mon, 12 Aug 2019 18:46:20: 2000000 INFO @ Mon, 12 Aug 2019 18:46:21: 2000000 INFO @ Mon, 12 Aug 2019 18:46:27: 3000000 INFO @ Mon, 12 Aug 2019 18:46:28: 3000000 INFO @ Mon, 12 Aug 2019 18:46:29: 3000000 INFO @ Mon, 12 Aug 2019 18:46:34: 4000000 INFO @ Mon, 12 Aug 2019 18:46:35: 4000000 INFO @ Mon, 12 Aug 2019 18:46:35: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:46:35: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:46:35: #1 total tags in treatment: 4214738 INFO @ Mon, 12 Aug 2019 18:46:35: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:46:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:46:35: #1 tags after filtering in treatment: 4214738 INFO @ Mon, 12 Aug 2019 18:46:35: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:46:35: #1 finished! INFO @ Mon, 12 Aug 2019 18:46:35: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:46:35: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:46:35: #2 number of paired peaks: 431 WARNING @ Mon, 12 Aug 2019 18:46:35: Fewer paired peaks (431) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 431 pairs to build model! INFO @ Mon, 12 Aug 2019 18:46:35: start model_add_line... INFO @ Mon, 12 Aug 2019 18:46:36: start X-correlation... INFO @ Mon, 12 Aug 2019 18:46:36: end of X-cor INFO @ Mon, 12 Aug 2019 18:46:36: #2 finished! INFO @ Mon, 12 Aug 2019 18:46:36: #2 predicted fragment length is 35 bps INFO @ Mon, 12 Aug 2019 18:46:36: #2 alternative fragment length(s) may be 4,35,70,88 bps INFO @ Mon, 12 Aug 2019 18:46:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.05_model.r WARNING @ Mon, 12 Aug 2019 18:46:36: #2 Since the d (35) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:46:36: #2 You may need to consider one of the other alternative d(s): 4,35,70,88 WARNING @ Mon, 12 Aug 2019 18:46:36: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:46:36: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:46:36: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:46:36: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:46:36: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:46:36: #1 total tags in treatment: 4214738 INFO @ Mon, 12 Aug 2019 18:46:36: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:46:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:46:36: #1 tags after filtering in treatment: 4214738 INFO @ Mon, 12 Aug 2019 18:46:36: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:46:36: #1 finished! INFO @ Mon, 12 Aug 2019 18:46:36: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:46:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:46:37: #2 number of paired peaks: 431 WARNING @ Mon, 12 Aug 2019 18:46:37: Fewer paired peaks (431) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 431 pairs to build model! INFO @ Mon, 12 Aug 2019 18:46:37: start model_add_line... INFO @ Mon, 12 Aug 2019 18:46:37: start X-correlation... INFO @ Mon, 12 Aug 2019 18:46:37: end of X-cor INFO @ Mon, 12 Aug 2019 18:46:37: #2 finished! INFO @ Mon, 12 Aug 2019 18:46:37: #2 predicted fragment length is 35 bps INFO @ Mon, 12 Aug 2019 18:46:37: #2 alternative fragment length(s) may be 4,35,70,88 bps INFO @ Mon, 12 Aug 2019 18:46:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.10_model.r WARNING @ Mon, 12 Aug 2019 18:46:37: #2 Since the d (35) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:46:37: #2 You may need to consider one of the other alternative d(s): 4,35,70,88 WARNING @ Mon, 12 Aug 2019 18:46:37: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:46:37: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:46:37: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:46:37: 4000000 INFO @ Mon, 12 Aug 2019 18:46:38: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:46:38: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:46:38: #1 total tags in treatment: 4214738 INFO @ Mon, 12 Aug 2019 18:46:38: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:46:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:46:39: #1 tags after filtering in treatment: 4214738 INFO @ Mon, 12 Aug 2019 18:46:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:46:39: #1 finished! INFO @ Mon, 12 Aug 2019 18:46:39: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:46:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:46:39: #2 number of paired peaks: 431 WARNING @ Mon, 12 Aug 2019 18:46:39: Fewer paired peaks (431) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 431 pairs to build model! INFO @ Mon, 12 Aug 2019 18:46:39: start model_add_line... INFO @ Mon, 12 Aug 2019 18:46:39: start X-correlation... INFO @ Mon, 12 Aug 2019 18:46:39: end of X-cor INFO @ Mon, 12 Aug 2019 18:46:39: #2 finished! INFO @ Mon, 12 Aug 2019 18:46:39: #2 predicted fragment length is 35 bps INFO @ Mon, 12 Aug 2019 18:46:39: #2 alternative fragment length(s) may be 4,35,70,88 bps INFO @ Mon, 12 Aug 2019 18:46:39: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.20_model.r WARNING @ Mon, 12 Aug 2019 18:46:39: #2 Since the d (35) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:46:39: #2 You may need to consider one of the other alternative d(s): 4,35,70,88 WARNING @ Mon, 12 Aug 2019 18:46:39: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:46:39: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:46:39: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:46:48: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:46:49: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:46:51: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:46:54: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.05_peaks.xls INFO @ Mon, 12 Aug 2019 18:46:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:46:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.05_summits.bed INFO @ Mon, 12 Aug 2019 18:46:54: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (363 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:46:55: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.10_peaks.xls INFO @ Mon, 12 Aug 2019 18:46:55: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:46:55: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.10_summits.bed INFO @ Mon, 12 Aug 2019 18:46:55: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (172 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:46:57: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.20_peaks.xls INFO @ Mon, 12 Aug 2019 18:46:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:46:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331313/SRX331313.20_summits.bed INFO @ Mon, 12 Aug 2019 18:46:57: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (55 records, 4 fields): 11 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。