Job ID = 2589849 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 7,662,619 reads read : 7,662,619 reads written : 7,662,619 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR947482.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:08 7662619 reads; of these: 7662619 (100.00%) were unpaired; of these: 3126768 (40.81%) aligned 0 times 3743229 (48.85%) aligned exactly 1 time 792622 (10.34%) aligned >1 times 59.19% overall alignment rate Time searching: 00:01:08 Overall time: 00:01:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 408832 / 4535851 = 0.0901 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 18:37:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:37:52: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:37:52: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:37:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:37:53: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:37:53: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:37:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:37:54: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:37:54: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:38:00: 1000000 INFO @ Mon, 12 Aug 2019 18:38:02: 1000000 INFO @ Mon, 12 Aug 2019 18:38:02: 1000000 INFO @ Mon, 12 Aug 2019 18:38:07: 2000000 INFO @ Mon, 12 Aug 2019 18:38:09: 2000000 INFO @ Mon, 12 Aug 2019 18:38:10: 2000000 INFO @ Mon, 12 Aug 2019 18:38:14: 3000000 INFO @ Mon, 12 Aug 2019 18:38:17: 3000000 INFO @ Mon, 12 Aug 2019 18:38:19: 3000000 INFO @ Mon, 12 Aug 2019 18:38:21: 4000000 INFO @ Mon, 12 Aug 2019 18:38:22: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:38:22: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:38:22: #1 total tags in treatment: 4127019 INFO @ Mon, 12 Aug 2019 18:38:22: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:38:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:38:22: #1 tags after filtering in treatment: 4127019 INFO @ Mon, 12 Aug 2019 18:38:22: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:38:22: #1 finished! INFO @ Mon, 12 Aug 2019 18:38:22: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:38:22: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:38:23: #2 number of paired peaks: 572 WARNING @ Mon, 12 Aug 2019 18:38:23: Fewer paired peaks (572) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 572 pairs to build model! INFO @ Mon, 12 Aug 2019 18:38:23: start model_add_line... INFO @ Mon, 12 Aug 2019 18:38:23: start X-correlation... INFO @ Mon, 12 Aug 2019 18:38:23: end of X-cor INFO @ Mon, 12 Aug 2019 18:38:23: #2 finished! INFO @ Mon, 12 Aug 2019 18:38:23: #2 predicted fragment length is 35 bps INFO @ Mon, 12 Aug 2019 18:38:23: #2 alternative fragment length(s) may be 3,35,86,574 bps INFO @ Mon, 12 Aug 2019 18:38:23: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.05_model.r WARNING @ Mon, 12 Aug 2019 18:38:23: #2 Since the d (35) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:38:23: #2 You may need to consider one of the other alternative d(s): 3,35,86,574 WARNING @ Mon, 12 Aug 2019 18:38:23: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:38:23: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:38:23: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:38:24: 4000000 INFO @ Mon, 12 Aug 2019 18:38:25: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:38:25: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:38:25: #1 total tags in treatment: 4127019 INFO @ Mon, 12 Aug 2019 18:38:25: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:38:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:38:25: #1 tags after filtering in treatment: 4127019 INFO @ Mon, 12 Aug 2019 18:38:25: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:38:25: #1 finished! INFO @ Mon, 12 Aug 2019 18:38:25: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:38:25: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:38:25: #2 number of paired peaks: 572 WARNING @ Mon, 12 Aug 2019 18:38:25: Fewer paired peaks (572) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 572 pairs to build model! INFO @ Mon, 12 Aug 2019 18:38:25: start model_add_line... INFO @ Mon, 12 Aug 2019 18:38:25: start X-correlation... INFO @ Mon, 12 Aug 2019 18:38:25: end of X-cor INFO @ Mon, 12 Aug 2019 18:38:25: #2 finished! INFO @ Mon, 12 Aug 2019 18:38:25: #2 predicted fragment length is 35 bps INFO @ Mon, 12 Aug 2019 18:38:25: #2 alternative fragment length(s) may be 3,35,86,574 bps INFO @ Mon, 12 Aug 2019 18:38:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.20_model.r WARNING @ Mon, 12 Aug 2019 18:38:25: #2 Since the d (35) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:38:25: #2 You may need to consider one of the other alternative d(s): 3,35,86,574 WARNING @ Mon, 12 Aug 2019 18:38:25: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:38:25: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:38:25: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:38:27: 4000000 INFO @ Mon, 12 Aug 2019 18:38:27: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:38:27: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:38:27: #1 total tags in treatment: 4127019 INFO @ Mon, 12 Aug 2019 18:38:27: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:38:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:38:28: #1 tags after filtering in treatment: 4127019 INFO @ Mon, 12 Aug 2019 18:38:28: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:38:28: #1 finished! INFO @ Mon, 12 Aug 2019 18:38:28: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:38:28: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:38:28: #2 number of paired peaks: 572 WARNING @ Mon, 12 Aug 2019 18:38:28: Fewer paired peaks (572) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 572 pairs to build model! INFO @ Mon, 12 Aug 2019 18:38:28: start model_add_line... INFO @ Mon, 12 Aug 2019 18:38:28: start X-correlation... INFO @ Mon, 12 Aug 2019 18:38:28: end of X-cor INFO @ Mon, 12 Aug 2019 18:38:28: #2 finished! INFO @ Mon, 12 Aug 2019 18:38:28: #2 predicted fragment length is 35 bps INFO @ Mon, 12 Aug 2019 18:38:28: #2 alternative fragment length(s) may be 3,35,86,574 bps INFO @ Mon, 12 Aug 2019 18:38:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.10_model.r WARNING @ Mon, 12 Aug 2019 18:38:28: #2 Since the d (35) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:38:28: #2 You may need to consider one of the other alternative d(s): 3,35,86,574 WARNING @ Mon, 12 Aug 2019 18:38:28: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:38:28: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:38:28: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:38:35: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:38:37: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:38:40: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:38:41: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.05_peaks.xls INFO @ Mon, 12 Aug 2019 18:38:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:38:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.05_summits.bed INFO @ Mon, 12 Aug 2019 18:38:41: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (577 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:38:43: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.20_peaks.xls INFO @ Mon, 12 Aug 2019 18:38:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:38:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.20_summits.bed INFO @ Mon, 12 Aug 2019 18:38:43: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (85 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:38:46: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.10_peaks.xls INFO @ Mon, 12 Aug 2019 18:38:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:38:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331250/SRX331250.10_summits.bed INFO @ Mon, 12 Aug 2019 18:38:47: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (252 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。