Job ID = 2589824 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 6,968,834 reads read : 6,968,834 reads written : 6,968,834 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR947453.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:14 6968834 reads; of these: 6968834 (100.00%) were unpaired; of these: 49463 (0.71%) aligned 0 times 5758596 (82.63%) aligned exactly 1 time 1160775 (16.66%) aligned >1 times 99.29% overall alignment rate Time searching: 00:01:14 Overall time: 00:01:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 498021 / 6919371 = 0.0720 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 18:34:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:34:57: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:34:57: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:34:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:34:58: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:34:58: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:34:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:34:59: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:34:59: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:35:04: 1000000 INFO @ Mon, 12 Aug 2019 18:35:07: 1000000 INFO @ Mon, 12 Aug 2019 18:35:07: 1000000 INFO @ Mon, 12 Aug 2019 18:35:12: 2000000 INFO @ Mon, 12 Aug 2019 18:35:14: 2000000 INFO @ Mon, 12 Aug 2019 18:35:15: 2000000 INFO @ Mon, 12 Aug 2019 18:35:19: 3000000 INFO @ Mon, 12 Aug 2019 18:35:22: 3000000 INFO @ Mon, 12 Aug 2019 18:35:24: 3000000 INFO @ Mon, 12 Aug 2019 18:35:26: 4000000 INFO @ Mon, 12 Aug 2019 18:35:29: 4000000 INFO @ Mon, 12 Aug 2019 18:35:32: 4000000 INFO @ Mon, 12 Aug 2019 18:35:34: 5000000 INFO @ Mon, 12 Aug 2019 18:35:36: 5000000 INFO @ Mon, 12 Aug 2019 18:35:41: 6000000 INFO @ Mon, 12 Aug 2019 18:35:41: 5000000 INFO @ Mon, 12 Aug 2019 18:35:44: 6000000 INFO @ Mon, 12 Aug 2019 18:35:44: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:35:44: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:35:44: #1 total tags in treatment: 6421350 INFO @ Mon, 12 Aug 2019 18:35:44: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:35:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:35:44: #1 tags after filtering in treatment: 6421350 INFO @ Mon, 12 Aug 2019 18:35:44: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:35:44: #1 finished! INFO @ Mon, 12 Aug 2019 18:35:44: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:35:44: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:35:45: #2 number of paired peaks: 380 WARNING @ Mon, 12 Aug 2019 18:35:45: Fewer paired peaks (380) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 380 pairs to build model! INFO @ Mon, 12 Aug 2019 18:35:45: start model_add_line... INFO @ Mon, 12 Aug 2019 18:35:45: start X-correlation... INFO @ Mon, 12 Aug 2019 18:35:45: end of X-cor INFO @ Mon, 12 Aug 2019 18:35:45: #2 finished! INFO @ Mon, 12 Aug 2019 18:35:45: #2 predicted fragment length is 29 bps INFO @ Mon, 12 Aug 2019 18:35:45: #2 alternative fragment length(s) may be 4,29,576 bps INFO @ Mon, 12 Aug 2019 18:35:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.05_model.r WARNING @ Mon, 12 Aug 2019 18:35:45: #2 Since the d (29) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:35:45: #2 You may need to consider one of the other alternative d(s): 4,29,576 WARNING @ Mon, 12 Aug 2019 18:35:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:35:45: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:35:45: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:35:47: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:35:47: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:35:47: #1 total tags in treatment: 6421350 INFO @ Mon, 12 Aug 2019 18:35:47: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:35:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:35:47: #1 tags after filtering in treatment: 6421350 INFO @ Mon, 12 Aug 2019 18:35:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:35:47: #1 finished! INFO @ Mon, 12 Aug 2019 18:35:47: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:35:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:35:47: #2 number of paired peaks: 380 WARNING @ Mon, 12 Aug 2019 18:35:47: Fewer paired peaks (380) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 380 pairs to build model! INFO @ Mon, 12 Aug 2019 18:35:47: start model_add_line... INFO @ Mon, 12 Aug 2019 18:35:48: start X-correlation... INFO @ Mon, 12 Aug 2019 18:35:48: end of X-cor INFO @ Mon, 12 Aug 2019 18:35:48: #2 finished! INFO @ Mon, 12 Aug 2019 18:35:48: #2 predicted fragment length is 29 bps INFO @ Mon, 12 Aug 2019 18:35:48: #2 alternative fragment length(s) may be 4,29,576 bps INFO @ Mon, 12 Aug 2019 18:35:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.20_model.r WARNING @ Mon, 12 Aug 2019 18:35:48: #2 Since the d (29) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:35:48: #2 You may need to consider one of the other alternative d(s): 4,29,576 WARNING @ Mon, 12 Aug 2019 18:35:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:35:48: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:35:48: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:35:49: 6000000 INFO @ Mon, 12 Aug 2019 18:35:53: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:35:53: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:35:53: #1 total tags in treatment: 6421350 INFO @ Mon, 12 Aug 2019 18:35:53: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:35:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:35:53: #1 tags after filtering in treatment: 6421350 INFO @ Mon, 12 Aug 2019 18:35:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:35:53: #1 finished! INFO @ Mon, 12 Aug 2019 18:35:53: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:35:53: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:35:53: #2 number of paired peaks: 380 WARNING @ Mon, 12 Aug 2019 18:35:53: Fewer paired peaks (380) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 380 pairs to build model! INFO @ Mon, 12 Aug 2019 18:35:53: start model_add_line... INFO @ Mon, 12 Aug 2019 18:35:53: start X-correlation... INFO @ Mon, 12 Aug 2019 18:35:53: end of X-cor INFO @ Mon, 12 Aug 2019 18:35:53: #2 finished! INFO @ Mon, 12 Aug 2019 18:35:53: #2 predicted fragment length is 29 bps INFO @ Mon, 12 Aug 2019 18:35:53: #2 alternative fragment length(s) may be 4,29,576 bps INFO @ Mon, 12 Aug 2019 18:35:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.10_model.r WARNING @ Mon, 12 Aug 2019 18:35:53: #2 Since the d (29) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:35:53: #2 You may need to consider one of the other alternative d(s): 4,29,576 WARNING @ Mon, 12 Aug 2019 18:35:53: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:35:53: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:35:53: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:36:03: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:36:06: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:36:11: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.05_peaks.xls INFO @ Mon, 12 Aug 2019 18:36:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:36:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.05_summits.bed INFO @ Mon, 12 Aug 2019 18:36:11: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (480 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:36:12: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:36:14: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.20_peaks.xls INFO @ Mon, 12 Aug 2019 18:36:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:36:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.20_summits.bed INFO @ Mon, 12 Aug 2019 18:36:14: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (69 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:36:20: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.10_peaks.xls INFO @ Mon, 12 Aug 2019 18:36:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:36:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331221/SRX331221.10_summits.bed INFO @ Mon, 12 Aug 2019 18:36:20: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (209 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。