Job ID = 1292125 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 6,892,318 reads read : 6,892,318 reads written : 6,892,318 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:16 6892318 reads; of these: 6892318 (100.00%) were unpaired; of these: 28139 (0.41%) aligned 0 times 5676138 (82.35%) aligned exactly 1 time 1188041 (17.24%) aligned >1 times 99.59% overall alignment rate Time searching: 00:01:16 Overall time: 00:01:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 427449 / 6864179 = 0.0623 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 17:32:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:32:04: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:32:04: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:32:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:32:04: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:32:04: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:32:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:32:04: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:32:04: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:32:11: 1000000 INFO @ Sun, 02 Jun 2019 17:32:11: 1000000 INFO @ Sun, 02 Jun 2019 17:32:12: 1000000 INFO @ Sun, 02 Jun 2019 17:32:17: 2000000 INFO @ Sun, 02 Jun 2019 17:32:18: 2000000 INFO @ Sun, 02 Jun 2019 17:32:20: 2000000 INFO @ Sun, 02 Jun 2019 17:32:23: 3000000 INFO @ Sun, 02 Jun 2019 17:32:24: 3000000 INFO @ Sun, 02 Jun 2019 17:32:27: 3000000 INFO @ Sun, 02 Jun 2019 17:32:29: 4000000 INFO @ Sun, 02 Jun 2019 17:32:30: 4000000 INFO @ Sun, 02 Jun 2019 17:32:34: 4000000 INFO @ Sun, 02 Jun 2019 17:32:35: 5000000 INFO @ Sun, 02 Jun 2019 17:32:37: 5000000 INFO @ Sun, 02 Jun 2019 17:32:41: 6000000 INFO @ Sun, 02 Jun 2019 17:32:41: 5000000 INFO @ Sun, 02 Jun 2019 17:32:43: 6000000 INFO @ Sun, 02 Jun 2019 17:32:43: #1 tag size is determined as 32 bps INFO @ Sun, 02 Jun 2019 17:32:43: #1 tag size = 32 INFO @ Sun, 02 Jun 2019 17:32:43: #1 total tags in treatment: 6436730 INFO @ Sun, 02 Jun 2019 17:32:43: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:32:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:32:43: #1 tags after filtering in treatment: 6436730 INFO @ Sun, 02 Jun 2019 17:32:43: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:32:43: #1 finished! INFO @ Sun, 02 Jun 2019 17:32:43: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:32:43: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:32:44: #2 number of paired peaks: 436 WARNING @ Sun, 02 Jun 2019 17:32:44: Fewer paired peaks (436) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 436 pairs to build model! INFO @ Sun, 02 Jun 2019 17:32:44: start model_add_line... INFO @ Sun, 02 Jun 2019 17:32:44: start X-correlation... INFO @ Sun, 02 Jun 2019 17:32:44: end of X-cor INFO @ Sun, 02 Jun 2019 17:32:44: #2 finished! INFO @ Sun, 02 Jun 2019 17:32:44: #2 predicted fragment length is 36 bps INFO @ Sun, 02 Jun 2019 17:32:44: #2 alternative fragment length(s) may be 3,36 bps INFO @ Sun, 02 Jun 2019 17:32:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.10_model.r WARNING @ Sun, 02 Jun 2019 17:32:44: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 17:32:44: #2 You may need to consider one of the other alternative d(s): 3,36 WARNING @ Sun, 02 Jun 2019 17:32:44: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 17:32:44: #3 Call peaks... INFO @ Sun, 02 Jun 2019 17:32:44: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 17:32:46: #1 tag size is determined as 32 bps INFO @ Sun, 02 Jun 2019 17:32:46: #1 tag size = 32 INFO @ Sun, 02 Jun 2019 17:32:46: #1 total tags in treatment: 6436730 INFO @ Sun, 02 Jun 2019 17:32:46: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:32:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:32:46: #1 tags after filtering in treatment: 6436730 INFO @ Sun, 02 Jun 2019 17:32:46: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:32:46: #1 finished! INFO @ Sun, 02 Jun 2019 17:32:46: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:32:46: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:32:47: #2 number of paired peaks: 436 WARNING @ Sun, 02 Jun 2019 17:32:47: Fewer paired peaks (436) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 436 pairs to build model! INFO @ Sun, 02 Jun 2019 17:32:47: start model_add_line... INFO @ Sun, 02 Jun 2019 17:32:47: start X-correlation... INFO @ Sun, 02 Jun 2019 17:32:47: end of X-cor INFO @ Sun, 02 Jun 2019 17:32:47: #2 finished! INFO @ Sun, 02 Jun 2019 17:32:47: #2 predicted fragment length is 36 bps INFO @ Sun, 02 Jun 2019 17:32:47: #2 alternative fragment length(s) may be 3,36 bps INFO @ Sun, 02 Jun 2019 17:32:47: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.05_model.r WARNING @ Sun, 02 Jun 2019 17:32:47: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 17:32:47: #2 You may need to consider one of the other alternative d(s): 3,36 WARNING @ Sun, 02 Jun 2019 17:32:47: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 17:32:47: #3 Call peaks... INFO @ Sun, 02 Jun 2019 17:32:47: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 17:32:49: 6000000 INFO @ Sun, 02 Jun 2019 17:32:52: #1 tag size is determined as 32 bps INFO @ Sun, 02 Jun 2019 17:32:52: #1 tag size = 32 INFO @ Sun, 02 Jun 2019 17:32:52: #1 total tags in treatment: 6436730 INFO @ Sun, 02 Jun 2019 17:32:52: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:32:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:32:52: #1 tags after filtering in treatment: 6436730 INFO @ Sun, 02 Jun 2019 17:32:52: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:32:52: #1 finished! INFO @ Sun, 02 Jun 2019 17:32:52: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:32:52: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:32:53: #2 number of paired peaks: 436 WARNING @ Sun, 02 Jun 2019 17:32:53: Fewer paired peaks (436) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 436 pairs to build model! INFO @ Sun, 02 Jun 2019 17:32:53: start model_add_line... INFO @ Sun, 02 Jun 2019 17:32:53: start X-correlation... INFO @ Sun, 02 Jun 2019 17:32:53: end of X-cor INFO @ Sun, 02 Jun 2019 17:32:53: #2 finished! INFO @ Sun, 02 Jun 2019 17:32:53: #2 predicted fragment length is 36 bps INFO @ Sun, 02 Jun 2019 17:32:53: #2 alternative fragment length(s) may be 3,36 bps INFO @ Sun, 02 Jun 2019 17:32:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.20_model.r WARNING @ Sun, 02 Jun 2019 17:32:53: #2 Since the d (36) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 17:32:53: #2 You may need to consider one of the other alternative d(s): 3,36 WARNING @ Sun, 02 Jun 2019 17:32:53: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 17:32:53: #3 Call peaks... INFO @ Sun, 02 Jun 2019 17:32:53: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 17:33:02: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 17:33:05: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 17:33:11: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 17:33:11: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.10_peaks.xls INFO @ Sun, 02 Jun 2019 17:33:11: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.10_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 17:33:11: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.10_summits.bed INFO @ Sun, 02 Jun 2019 17:33:11: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (259 records, 4 fields): 21 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 17:33:14: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.05_peaks.xls INFO @ Sun, 02 Jun 2019 17:33:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.05_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 17:33:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.05_summits.bed INFO @ Sun, 02 Jun 2019 17:33:14: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (564 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 17:33:20: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.20_peaks.xls INFO @ Sun, 02 Jun 2019 17:33:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.20_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 17:33:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331163/SRX331163.20_summits.bed INFO @ Sun, 02 Jun 2019 17:33:20: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (82 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。