Job ID = 2589762 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 6,142,831 reads read : 6,142,831 reads written : 6,142,831 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR947367.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:27 6142831 reads; of these: 6142831 (100.00%) were unpaired; of these: 5476688 (89.16%) aligned 0 times 567640 (9.24%) aligned exactly 1 time 98503 (1.60%) aligned >1 times 10.84% overall alignment rate Time searching: 00:00:27 Overall time: 00:00:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 110989 / 666143 = 0.1666 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 18:24:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:24:40: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:24:40: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:24:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:24:41: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:24:41: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:24:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:24:42: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:24:42: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:24:45: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:24:45: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:24:45: #1 total tags in treatment: 555154 INFO @ Mon, 12 Aug 2019 18:24:45: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:24:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:24:45: #1 tags after filtering in treatment: 555154 INFO @ Mon, 12 Aug 2019 18:24:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:24:45: #1 finished! INFO @ Mon, 12 Aug 2019 18:24:45: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:24:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:24:45: #2 number of paired peaks: 913 WARNING @ Mon, 12 Aug 2019 18:24:45: Fewer paired peaks (913) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 913 pairs to build model! INFO @ Mon, 12 Aug 2019 18:24:45: start model_add_line... INFO @ Mon, 12 Aug 2019 18:24:45: start X-correlation... INFO @ Mon, 12 Aug 2019 18:24:45: end of X-cor INFO @ Mon, 12 Aug 2019 18:24:45: #2 finished! INFO @ Mon, 12 Aug 2019 18:24:45: #2 predicted fragment length is 119 bps INFO @ Mon, 12 Aug 2019 18:24:45: #2 alternative fragment length(s) may be 119,589 bps INFO @ Mon, 12 Aug 2019 18:24:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.10_model.r INFO @ Mon, 12 Aug 2019 18:24:45: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:24:45: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:24:45: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:24:45: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:24:45: #1 total tags in treatment: 555154 INFO @ Mon, 12 Aug 2019 18:24:45: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:24:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:24:45: #1 tags after filtering in treatment: 555154 INFO @ Mon, 12 Aug 2019 18:24:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:24:45: #1 finished! INFO @ Mon, 12 Aug 2019 18:24:45: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:24:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:24:45: #2 number of paired peaks: 913 WARNING @ Mon, 12 Aug 2019 18:24:45: Fewer paired peaks (913) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 913 pairs to build model! INFO @ Mon, 12 Aug 2019 18:24:45: start model_add_line... INFO @ Mon, 12 Aug 2019 18:24:45: start X-correlation... INFO @ Mon, 12 Aug 2019 18:24:45: end of X-cor INFO @ Mon, 12 Aug 2019 18:24:45: #2 finished! INFO @ Mon, 12 Aug 2019 18:24:45: #2 predicted fragment length is 119 bps INFO @ Mon, 12 Aug 2019 18:24:45: #2 alternative fragment length(s) may be 119,589 bps INFO @ Mon, 12 Aug 2019 18:24:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.05_model.r INFO @ Mon, 12 Aug 2019 18:24:45: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:24:45: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:24:45: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:24:45: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:24:45: #1 total tags in treatment: 555154 INFO @ Mon, 12 Aug 2019 18:24:45: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:24:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:24:45: #1 tags after filtering in treatment: 555154 INFO @ Mon, 12 Aug 2019 18:24:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:24:45: #1 finished! INFO @ Mon, 12 Aug 2019 18:24:45: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:24:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:24:46: #2 number of paired peaks: 913 WARNING @ Mon, 12 Aug 2019 18:24:46: Fewer paired peaks (913) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 913 pairs to build model! INFO @ Mon, 12 Aug 2019 18:24:46: start model_add_line... INFO @ Mon, 12 Aug 2019 18:24:46: start X-correlation... INFO @ Mon, 12 Aug 2019 18:24:46: end of X-cor INFO @ Mon, 12 Aug 2019 18:24:46: #2 finished! INFO @ Mon, 12 Aug 2019 18:24:46: #2 predicted fragment length is 119 bps INFO @ Mon, 12 Aug 2019 18:24:46: #2 alternative fragment length(s) may be 119,589 bps INFO @ Mon, 12 Aug 2019 18:24:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.20_model.r INFO @ Mon, 12 Aug 2019 18:24:46: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:24:46: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:24:47: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:24:47: #3 Call peaks for each chromosome... BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 12 Aug 2019 18:24:48: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:24:48: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.10_peaks.xls INFO @ Mon, 12 Aug 2019 18:24:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:24:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.10_summits.bed INFO @ Mon, 12 Aug 2019 18:24:48: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (86 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:24:48: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.05_peaks.xls INFO @ Mon, 12 Aug 2019 18:24:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:24:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.05_summits.bed INFO @ Mon, 12 Aug 2019 18:24:48: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (316 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:24:48: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.20_peaks.xls INFO @ Mon, 12 Aug 2019 18:24:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:24:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX331136/SRX331136.20_summits.bed INFO @ Mon, 12 Aug 2019 18:24:48: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (24 records, 4 fields): 2 millis CompletedMACS2peakCalling BigWig に変換しました。