Job ID = 1292045 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 16,048,349 reads read : 16,048,349 reads written : 16,048,349 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:05:05 16048349 reads; of these: 16048349 (100.00%) were unpaired; of these: 1683028 (10.49%) aligned 0 times 12466975 (77.68%) aligned exactly 1 time 1898346 (11.83%) aligned >1 times 89.51% overall alignment rate Time searching: 00:05:06 Overall time: 00:05:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3935514 / 14365321 = 0.2740 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 17:30:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:30:56: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:30:56: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:30:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:30:56: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:30:56: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:30:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:30:56: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:30:56: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:31:05: 1000000 INFO @ Sun, 02 Jun 2019 17:31:07: 1000000 INFO @ Sun, 02 Jun 2019 17:31:07: 1000000 INFO @ Sun, 02 Jun 2019 17:31:13: 2000000 INFO @ Sun, 02 Jun 2019 17:31:17: 2000000 INFO @ Sun, 02 Jun 2019 17:31:18: 2000000 INFO @ Sun, 02 Jun 2019 17:31:22: 3000000 INFO @ Sun, 02 Jun 2019 17:31:28: 3000000 INFO @ Sun, 02 Jun 2019 17:31:30: 3000000 INFO @ Sun, 02 Jun 2019 17:31:31: 4000000 INFO @ Sun, 02 Jun 2019 17:31:38: 4000000 INFO @ Sun, 02 Jun 2019 17:31:40: 5000000 INFO @ Sun, 02 Jun 2019 17:31:43: 4000000 INFO @ Sun, 02 Jun 2019 17:31:48: 5000000 INFO @ Sun, 02 Jun 2019 17:31:48: 6000000 INFO @ Sun, 02 Jun 2019 17:31:56: 5000000 INFO @ Sun, 02 Jun 2019 17:31:57: 7000000 INFO @ Sun, 02 Jun 2019 17:31:58: 6000000 INFO @ Sun, 02 Jun 2019 17:32:06: 8000000 INFO @ Sun, 02 Jun 2019 17:32:07: 7000000 INFO @ Sun, 02 Jun 2019 17:32:09: 6000000 INFO @ Sun, 02 Jun 2019 17:32:15: 9000000 INFO @ Sun, 02 Jun 2019 17:32:17: 8000000 INFO @ Sun, 02 Jun 2019 17:32:22: 7000000 INFO @ Sun, 02 Jun 2019 17:32:24: 10000000 INFO @ Sun, 02 Jun 2019 17:32:27: 9000000 INFO @ Sun, 02 Jun 2019 17:32:27: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 17:32:27: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 17:32:27: #1 total tags in treatment: 10429807 INFO @ Sun, 02 Jun 2019 17:32:27: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:32:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:32:28: #1 tags after filtering in treatment: 10429807 INFO @ Sun, 02 Jun 2019 17:32:28: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:32:28: #1 finished! INFO @ Sun, 02 Jun 2019 17:32:28: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:32:28: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:32:29: #2 number of paired peaks: 47 WARNING @ Sun, 02 Jun 2019 17:32:29: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:32:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 17:32:36: 8000000 INFO @ Sun, 02 Jun 2019 17:32:37: 10000000 INFO @ Sun, 02 Jun 2019 17:32:41: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 17:32:41: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 17:32:41: #1 total tags in treatment: 10429807 INFO @ Sun, 02 Jun 2019 17:32:41: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:32:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:32:41: #1 tags after filtering in treatment: 10429807 INFO @ Sun, 02 Jun 2019 17:32:41: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:32:41: #1 finished! INFO @ Sun, 02 Jun 2019 17:32:41: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:32:41: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:32:42: #2 number of paired peaks: 47 WARNING @ Sun, 02 Jun 2019 17:32:42: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:32:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 17:32:49: 9000000 INFO @ Sun, 02 Jun 2019 17:33:02: 10000000 INFO @ Sun, 02 Jun 2019 17:33:08: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 17:33:08: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 17:33:08: #1 total tags in treatment: 10429807 INFO @ Sun, 02 Jun 2019 17:33:08: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:33:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:33:08: #1 tags after filtering in treatment: 10429807 INFO @ Sun, 02 Jun 2019 17:33:08: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:33:08: #1 finished! INFO @ Sun, 02 Jun 2019 17:33:08: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:33:08: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:33:09: #2 number of paired peaks: 47 WARNING @ Sun, 02 Jun 2019 17:33:09: Too few paired peaks (47) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:33:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323691/SRX323691.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。