Job ID = 1292033 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 15,072,086 reads read : 15,072,086 reads written : 15,072,086 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:53 15072086 reads; of these: 15072086 (100.00%) were unpaired; of these: 839386 (5.57%) aligned 0 times 12128284 (80.47%) aligned exactly 1 time 2104416 (13.96%) aligned >1 times 94.43% overall alignment rate Time searching: 00:04:53 Overall time: 00:04:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3508552 / 14232700 = 0.2465 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 17:28:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:28:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:28:12: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:28:12: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:28:12: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:28:12: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:28:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 17:28:12: #1 read tag files... INFO @ Sun, 02 Jun 2019 17:28:12: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 17:28:24: 1000000 INFO @ Sun, 02 Jun 2019 17:28:25: 1000000 INFO @ Sun, 02 Jun 2019 17:28:25: 1000000 INFO @ Sun, 02 Jun 2019 17:28:35: 2000000 INFO @ Sun, 02 Jun 2019 17:28:37: 2000000 INFO @ Sun, 02 Jun 2019 17:28:37: 2000000 INFO @ Sun, 02 Jun 2019 17:28:46: 3000000 INFO @ Sun, 02 Jun 2019 17:28:49: 3000000 INFO @ Sun, 02 Jun 2019 17:28:49: 3000000 INFO @ Sun, 02 Jun 2019 17:28:56: 4000000 INFO @ Sun, 02 Jun 2019 17:29:01: 4000000 INFO @ Sun, 02 Jun 2019 17:29:02: 4000000 INFO @ Sun, 02 Jun 2019 17:29:07: 5000000 INFO @ Sun, 02 Jun 2019 17:29:13: 5000000 INFO @ Sun, 02 Jun 2019 17:29:14: 5000000 INFO @ Sun, 02 Jun 2019 17:29:18: 6000000 INFO @ Sun, 02 Jun 2019 17:29:26: 6000000 INFO @ Sun, 02 Jun 2019 17:29:26: 6000000 INFO @ Sun, 02 Jun 2019 17:29:29: 7000000 INFO @ Sun, 02 Jun 2019 17:29:38: 7000000 INFO @ Sun, 02 Jun 2019 17:29:39: 7000000 INFO @ Sun, 02 Jun 2019 17:29:40: 8000000 INFO @ Sun, 02 Jun 2019 17:29:50: 8000000 INFO @ Sun, 02 Jun 2019 17:29:50: 8000000 INFO @ Sun, 02 Jun 2019 17:29:51: 9000000 INFO @ Sun, 02 Jun 2019 17:30:02: 9000000 INFO @ Sun, 02 Jun 2019 17:30:02: 10000000 INFO @ Sun, 02 Jun 2019 17:30:02: 9000000 INFO @ Sun, 02 Jun 2019 17:30:09: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 17:30:09: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 17:30:09: #1 total tags in treatment: 10724148 INFO @ Sun, 02 Jun 2019 17:30:09: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:30:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:30:10: #1 tags after filtering in treatment: 10724148 INFO @ Sun, 02 Jun 2019 17:30:10: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:30:10: #1 finished! INFO @ Sun, 02 Jun 2019 17:30:10: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:30:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:30:11: #2 number of paired peaks: 42 WARNING @ Sun, 02 Jun 2019 17:30:11: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:30:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 17:30:14: 10000000 INFO @ Sun, 02 Jun 2019 17:30:14: 10000000 INFO @ Sun, 02 Jun 2019 17:30:22: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 17:30:22: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 17:30:22: #1 total tags in treatment: 10724148 INFO @ Sun, 02 Jun 2019 17:30:22: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:30:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:30:22: #1 tags after filtering in treatment: 10724148 INFO @ Sun, 02 Jun 2019 17:30:22: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:30:22: #1 finished! INFO @ Sun, 02 Jun 2019 17:30:22: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:30:22: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:30:22: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 17:30:22: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 17:30:22: #1 total tags in treatment: 10724148 INFO @ Sun, 02 Jun 2019 17:30:22: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 17:30:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 17:30:23: #1 tags after filtering in treatment: 10724148 INFO @ Sun, 02 Jun 2019 17:30:23: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 17:30:23: #1 finished! INFO @ Sun, 02 Jun 2019 17:30:23: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 17:30:23: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 17:30:23: #2 number of paired peaks: 42 WARNING @ Sun, 02 Jun 2019 17:30:23: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:30:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 17:30:24: #2 number of paired peaks: 42 WARNING @ Sun, 02 Jun 2019 17:30:24: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 17:30:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX323679/SRX323679.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。