Job ID = 14159675 SRX = SRX3180794 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 2440123 spots for SRR6030445/SRR6030445.sra Written 2440123 spots for SRR6030445/SRR6030445.sra fastq に変換しました。 bowtie でマッピング中... Your job 14160273 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:30 2440123 reads; of these: 2440123 (100.00%) were unpaired; of these: 575746 (23.59%) aligned 0 times 1543115 (63.24%) aligned exactly 1 time 321262 (13.17%) aligned >1 times 76.41% overall alignment rate Time searching: 00:00:30 Overall time: 00:00:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 1232184 / 1864377 = 0.6609 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 00:15:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 00:15:46: #1 read tag files... INFO @ Thu, 09 Dec 2021 00:15:46: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 00:15:50: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 00:15:50: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 00:15:50: #1 total tags in treatment: 632193 INFO @ Thu, 09 Dec 2021 00:15:50: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 00:15:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 00:15:50: #1 tags after filtering in treatment: 632193 INFO @ Thu, 09 Dec 2021 00:15:50: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 00:15:50: #1 finished! INFO @ Thu, 09 Dec 2021 00:15:50: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 00:15:50: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 00:15:50: #2 number of paired peaks: 593 WARNING @ Thu, 09 Dec 2021 00:15:50: Fewer paired peaks (593) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 593 pairs to build model! INFO @ Thu, 09 Dec 2021 00:15:50: start model_add_line... INFO @ Thu, 09 Dec 2021 00:15:50: start X-correlation... INFO @ Thu, 09 Dec 2021 00:15:50: end of X-cor INFO @ Thu, 09 Dec 2021 00:15:50: #2 finished! INFO @ Thu, 09 Dec 2021 00:15:50: #2 predicted fragment length is 50 bps INFO @ Thu, 09 Dec 2021 00:15:50: #2 alternative fragment length(s) may be 50,580 bps INFO @ Thu, 09 Dec 2021 00:15:50: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.05_model.r WARNING @ Thu, 09 Dec 2021 00:15:50: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 00:15:50: #2 You may need to consider one of the other alternative d(s): 50,580 WARNING @ Thu, 09 Dec 2021 00:15:50: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 00:15:50: #3 Call peaks... INFO @ Thu, 09 Dec 2021 00:15:50: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 00:15:52: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 00:15:52: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.05_peaks.xls INFO @ Thu, 09 Dec 2021 00:15:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 00:15:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.05_summits.bed INFO @ Thu, 09 Dec 2021 00:15:53: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (295 records, 4 fields): 2 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 00:16:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 00:16:16: #1 read tag files... INFO @ Thu, 09 Dec 2021 00:16:16: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 00:16:20: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 00:16:20: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 00:16:20: #1 total tags in treatment: 632193 INFO @ Thu, 09 Dec 2021 00:16:20: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 00:16:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 00:16:20: #1 tags after filtering in treatment: 632193 INFO @ Thu, 09 Dec 2021 00:16:20: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 00:16:20: #1 finished! INFO @ Thu, 09 Dec 2021 00:16:20: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 00:16:20: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 00:16:20: #2 number of paired peaks: 593 WARNING @ Thu, 09 Dec 2021 00:16:20: Fewer paired peaks (593) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 593 pairs to build model! INFO @ Thu, 09 Dec 2021 00:16:20: start model_add_line... INFO @ Thu, 09 Dec 2021 00:16:20: start X-correlation... INFO @ Thu, 09 Dec 2021 00:16:20: end of X-cor INFO @ Thu, 09 Dec 2021 00:16:20: #2 finished! INFO @ Thu, 09 Dec 2021 00:16:20: #2 predicted fragment length is 50 bps INFO @ Thu, 09 Dec 2021 00:16:20: #2 alternative fragment length(s) may be 50,580 bps INFO @ Thu, 09 Dec 2021 00:16:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.10_model.r WARNING @ Thu, 09 Dec 2021 00:16:20: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 00:16:20: #2 You may need to consider one of the other alternative d(s): 50,580 WARNING @ Thu, 09 Dec 2021 00:16:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 00:16:20: #3 Call peaks... INFO @ Thu, 09 Dec 2021 00:16:20: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 00:16:21: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 00:16:22: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.10_peaks.xls INFO @ Thu, 09 Dec 2021 00:16:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 00:16:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.10_summits.bed INFO @ Thu, 09 Dec 2021 00:16:22: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (127 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 00:16:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 00:16:47: #1 read tag files... INFO @ Thu, 09 Dec 2021 00:16:47: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 00:16:51: #1 tag size is determined as 51 bps INFO @ Thu, 09 Dec 2021 00:16:51: #1 tag size = 51 INFO @ Thu, 09 Dec 2021 00:16:51: #1 total tags in treatment: 632193 INFO @ Thu, 09 Dec 2021 00:16:51: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 00:16:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 00:16:51: #1 tags after filtering in treatment: 632193 INFO @ Thu, 09 Dec 2021 00:16:51: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 00:16:51: #1 finished! INFO @ Thu, 09 Dec 2021 00:16:51: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 00:16:51: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 00:16:51: #2 number of paired peaks: 593 WARNING @ Thu, 09 Dec 2021 00:16:51: Fewer paired peaks (593) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 593 pairs to build model! INFO @ Thu, 09 Dec 2021 00:16:51: start model_add_line... INFO @ Thu, 09 Dec 2021 00:16:51: start X-correlation... INFO @ Thu, 09 Dec 2021 00:16:51: end of X-cor INFO @ Thu, 09 Dec 2021 00:16:51: #2 finished! INFO @ Thu, 09 Dec 2021 00:16:51: #2 predicted fragment length is 50 bps INFO @ Thu, 09 Dec 2021 00:16:51: #2 alternative fragment length(s) may be 50,580 bps INFO @ Thu, 09 Dec 2021 00:16:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.20_model.r WARNING @ Thu, 09 Dec 2021 00:16:51: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 00:16:51: #2 You may need to consider one of the other alternative d(s): 50,580 WARNING @ Thu, 09 Dec 2021 00:16:51: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 00:16:51: #3 Call peaks... INFO @ Thu, 09 Dec 2021 00:16:51: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 00:16:52: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 00:16:53: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.20_peaks.xls INFO @ Thu, 09 Dec 2021 00:16:53: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 00:16:53: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX3180794/SRX3180794.20_summits.bed INFO @ Thu, 09 Dec 2021 00:16:53: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (28 records, 4 fields): 1 millis CompletedMACS2peakCalling