Job ID = 12264765 SRX = SRX3029130 Genome = ce10 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 49621886 spots for SRR5860430/SRR5860430.sra Written 49621886 spots for SRR5860430/SRR5860430.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265584 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:52:16 49621886 reads; of these: 49621886 (100.00%) were paired; of these: 18546323 (37.38%) aligned concordantly 0 times 27220805 (54.86%) aligned concordantly exactly 1 time 3854758 (7.77%) aligned concordantly >1 times ---- 18546323 pairs aligned concordantly 0 times; of these: 5323854 (28.71%) aligned discordantly 1 time ---- 13222469 pairs aligned 0 times concordantly or discordantly; of these: 26444938 mates make up the pairs; of these: 23943200 (90.54%) aligned 0 times 1145793 (4.33%) aligned exactly 1 time 1355945 (5.13%) aligned >1 times 75.87% overall alignment rate Time searching: 00:52:17 Overall time: 00:52:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 8195411 / 35986559 = 0.2277 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:50:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:50:23: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:50:23: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:50:30: 1000000 INFO @ Sat, 03 Apr 2021 07:50:37: 2000000 INFO @ Sat, 03 Apr 2021 07:50:44: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:50:51: 4000000 INFO @ Sat, 03 Apr 2021 07:50:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:50:53: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:50:53: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:50:59: 5000000 INFO @ Sat, 03 Apr 2021 07:51:03: 1000000 INFO @ Sat, 03 Apr 2021 07:51:06: 6000000 INFO @ Sat, 03 Apr 2021 07:51:13: 7000000 INFO @ Sat, 03 Apr 2021 07:51:14: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:51:20: 8000000 INFO @ Sat, 03 Apr 2021 07:51:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:51:22: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:51:22: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:51:25: 3000000 INFO @ Sat, 03 Apr 2021 07:51:28: 9000000 INFO @ Sat, 03 Apr 2021 07:51:31: 1000000 INFO @ Sat, 03 Apr 2021 07:51:36: 4000000 INFO @ Sat, 03 Apr 2021 07:51:37: 10000000 INFO @ Sat, 03 Apr 2021 07:51:40: 2000000 INFO @ Sat, 03 Apr 2021 07:51:45: 11000000 INFO @ Sat, 03 Apr 2021 07:51:47: 5000000 INFO @ Sat, 03 Apr 2021 07:51:49: 3000000 INFO @ Sat, 03 Apr 2021 07:51:54: 12000000 INFO @ Sat, 03 Apr 2021 07:51:57: 4000000 INFO @ Sat, 03 Apr 2021 07:51:58: 6000000 INFO @ Sat, 03 Apr 2021 07:52:03: 13000000 INFO @ Sat, 03 Apr 2021 07:52:05: 5000000 INFO @ Sat, 03 Apr 2021 07:52:09: 7000000 INFO @ Sat, 03 Apr 2021 07:52:11: 14000000 INFO @ Sat, 03 Apr 2021 07:52:13: 6000000 INFO @ Sat, 03 Apr 2021 07:52:19: 15000000 INFO @ Sat, 03 Apr 2021 07:52:20: 8000000 INFO @ Sat, 03 Apr 2021 07:52:22: 7000000 INFO @ Sat, 03 Apr 2021 07:52:28: 16000000 INFO @ Sat, 03 Apr 2021 07:52:30: 8000000 INFO @ Sat, 03 Apr 2021 07:52:31: 9000000 INFO @ Sat, 03 Apr 2021 07:52:36: 17000000 INFO @ Sat, 03 Apr 2021 07:52:39: 9000000 INFO @ Sat, 03 Apr 2021 07:52:41: 10000000 INFO @ Sat, 03 Apr 2021 07:52:44: 18000000 INFO @ Sat, 03 Apr 2021 07:52:47: 10000000 INFO @ Sat, 03 Apr 2021 07:52:51: 11000000 INFO @ Sat, 03 Apr 2021 07:52:53: 19000000 INFO @ Sat, 03 Apr 2021 07:52:55: 11000000 INFO @ Sat, 03 Apr 2021 07:53:01: 20000000 INFO @ Sat, 03 Apr 2021 07:53:02: 12000000 INFO @ Sat, 03 Apr 2021 07:53:04: 12000000 INFO @ Sat, 03 Apr 2021 07:53:09: 21000000 INFO @ Sat, 03 Apr 2021 07:53:12: 13000000 INFO @ Sat, 03 Apr 2021 07:53:13: 13000000 INFO @ Sat, 03 Apr 2021 07:53:17: 22000000 INFO @ Sat, 03 Apr 2021 07:53:21: 14000000 INFO @ Sat, 03 Apr 2021 07:53:22: 14000000 INFO @ Sat, 03 Apr 2021 07:53:26: 23000000 INFO @ Sat, 03 Apr 2021 07:53:29: 15000000 INFO @ Sat, 03 Apr 2021 07:53:31: 15000000 INFO @ Sat, 03 Apr 2021 07:53:34: 24000000 INFO @ Sat, 03 Apr 2021 07:53:37: 16000000 INFO @ Sat, 03 Apr 2021 07:53:41: 16000000 INFO @ Sat, 03 Apr 2021 07:53:43: 25000000 INFO @ Sat, 03 Apr 2021 07:53:46: 17000000 INFO @ Sat, 03 Apr 2021 07:53:50: 17000000 INFO @ Sat, 03 Apr 2021 07:53:51: 26000000 INFO @ Sat, 03 Apr 2021 07:53:54: 18000000 INFO @ Sat, 03 Apr 2021 07:53:58: 18000000 INFO @ Sat, 03 Apr 2021 07:53:59: 27000000 INFO @ Sat, 03 Apr 2021 07:54:02: 19000000 INFO @ Sat, 03 Apr 2021 07:54:06: 19000000 INFO @ Sat, 03 Apr 2021 07:54:07: 28000000 INFO @ Sat, 03 Apr 2021 07:54:11: 20000000 INFO @ Sat, 03 Apr 2021 07:54:14: 20000000 INFO @ Sat, 03 Apr 2021 07:54:16: 29000000 INFO @ Sat, 03 Apr 2021 07:54:19: 21000000 INFO @ Sat, 03 Apr 2021 07:54:22: 21000000 INFO @ Sat, 03 Apr 2021 07:54:24: 30000000 INFO @ Sat, 03 Apr 2021 07:54:28: 22000000 INFO @ Sat, 03 Apr 2021 07:54:31: 22000000 INFO @ Sat, 03 Apr 2021 07:54:32: 31000000 INFO @ Sat, 03 Apr 2021 07:54:36: 23000000 INFO @ Sat, 03 Apr 2021 07:54:38: 23000000 INFO @ Sat, 03 Apr 2021 07:54:41: 32000000 INFO @ Sat, 03 Apr 2021 07:54:44: 24000000 INFO @ Sat, 03 Apr 2021 07:54:47: 24000000 INFO @ Sat, 03 Apr 2021 07:54:49: 33000000 INFO @ Sat, 03 Apr 2021 07:54:52: 25000000 INFO @ Sat, 03 Apr 2021 07:54:54: 25000000 INFO @ Sat, 03 Apr 2021 07:54:57: 34000000 INFO @ Sat, 03 Apr 2021 07:55:01: 26000000 INFO @ Sat, 03 Apr 2021 07:55:02: 26000000 INFO @ Sat, 03 Apr 2021 07:55:05: 35000000 INFO @ Sat, 03 Apr 2021 07:55:09: 27000000 INFO @ Sat, 03 Apr 2021 07:55:10: 27000000 INFO @ Sat, 03 Apr 2021 07:55:14: 36000000 INFO @ Sat, 03 Apr 2021 07:55:18: 28000000 INFO @ Sat, 03 Apr 2021 07:55:20: 28000000 INFO @ Sat, 03 Apr 2021 07:55:22: 37000000 INFO @ Sat, 03 Apr 2021 07:55:26: 29000000 INFO @ Sat, 03 Apr 2021 07:55:27: 29000000 INFO @ Sat, 03 Apr 2021 07:55:30: 38000000 INFO @ Sat, 03 Apr 2021 07:55:34: 30000000 INFO @ Sat, 03 Apr 2021 07:55:35: 30000000 INFO @ Sat, 03 Apr 2021 07:55:38: 39000000 INFO @ Sat, 03 Apr 2021 07:55:43: 31000000 INFO @ Sat, 03 Apr 2021 07:55:44: 31000000 INFO @ Sat, 03 Apr 2021 07:55:47: 40000000 INFO @ Sat, 03 Apr 2021 07:55:51: 32000000 INFO @ Sat, 03 Apr 2021 07:55:53: 32000000 INFO @ Sat, 03 Apr 2021 07:55:55: 41000000 INFO @ Sat, 03 Apr 2021 07:55:59: 33000000 INFO @ Sat, 03 Apr 2021 07:56:01: 33000000 INFO @ Sat, 03 Apr 2021 07:56:03: 42000000 INFO @ Sat, 03 Apr 2021 07:56:08: 34000000 INFO @ Sat, 03 Apr 2021 07:56:09: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:56:12: 43000000 INFO @ Sat, 03 Apr 2021 07:56:16: 35000000 INFO @ Sat, 03 Apr 2021 07:56:17: 35000000 INFO @ Sat, 03 Apr 2021 07:56:20: 44000000 INFO @ Sat, 03 Apr 2021 07:56:24: 36000000 INFO @ Sat, 03 Apr 2021 07:56:25: 36000000 INFO @ Sat, 03 Apr 2021 07:56:28: 45000000 INFO @ Sat, 03 Apr 2021 07:56:33: 37000000 INFO @ Sat, 03 Apr 2021 07:56:33: 37000000 INFO @ Sat, 03 Apr 2021 07:56:36: 46000000 INFO @ Sat, 03 Apr 2021 07:56:41: 38000000 INFO @ Sat, 03 Apr 2021 07:56:41: 38000000 INFO @ Sat, 03 Apr 2021 07:56:44: 47000000 INFO @ Sat, 03 Apr 2021 07:56:49: 39000000 INFO @ Sat, 03 Apr 2021 07:56:49: 39000000 INFO @ Sat, 03 Apr 2021 07:56:52: 48000000 INFO @ Sat, 03 Apr 2021 07:56:57: 40000000 INFO @ Sat, 03 Apr 2021 07:56:58: 40000000 INFO @ Sat, 03 Apr 2021 07:57:01: 49000000 INFO @ Sat, 03 Apr 2021 07:57:05: 41000000 INFO @ Sat, 03 Apr 2021 07:57:05: 41000000 INFO @ Sat, 03 Apr 2021 07:57:09: 50000000 INFO @ Sat, 03 Apr 2021 07:57:13: 42000000 INFO @ Sat, 03 Apr 2021 07:57:14: 42000000 INFO @ Sat, 03 Apr 2021 07:57:18: 51000000 INFO @ Sat, 03 Apr 2021 07:57:21: 43000000 INFO @ Sat, 03 Apr 2021 07:57:21: 43000000 INFO @ Sat, 03 Apr 2021 07:57:26: 52000000 INFO @ Sat, 03 Apr 2021 07:57:29: 44000000 INFO @ Sat, 03 Apr 2021 07:57:30: 44000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 07:57:34: 53000000 INFO @ Sat, 03 Apr 2021 07:57:36: 45000000 INFO @ Sat, 03 Apr 2021 07:57:38: 45000000 INFO @ Sat, 03 Apr 2021 07:57:42: 54000000 INFO @ Sat, 03 Apr 2021 07:57:44: 46000000 INFO @ Sat, 03 Apr 2021 07:57:46: 46000000 INFO @ Sat, 03 Apr 2021 07:57:51: 55000000 INFO @ Sat, 03 Apr 2021 07:57:52: 47000000 INFO @ Sat, 03 Apr 2021 07:57:55: 47000000 INFO @ Sat, 03 Apr 2021 07:58:00: 56000000 INFO @ Sat, 03 Apr 2021 07:58:01: 48000000 INFO @ Sat, 03 Apr 2021 07:58:03: 48000000 INFO @ Sat, 03 Apr 2021 07:58:08: 57000000 INFO @ Sat, 03 Apr 2021 07:58:09: 49000000 INFO @ Sat, 03 Apr 2021 07:58:12: 49000000 INFO @ Sat, 03 Apr 2021 07:58:16: 58000000 INFO @ Sat, 03 Apr 2021 07:58:17: 50000000 INFO @ Sat, 03 Apr 2021 07:58:20: 50000000 INFO @ Sat, 03 Apr 2021 07:58:24: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 07:58:24: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 07:58:24: #1 total tags in treatment: 24213865 INFO @ Sat, 03 Apr 2021 07:58:24: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:58:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:58:25: #1 tags after filtering in treatment: 16701370 INFO @ Sat, 03 Apr 2021 07:58:25: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 03 Apr 2021 07:58:25: #1 finished! INFO @ Sat, 03 Apr 2021 07:58:25: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:58:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:58:25: 51000000 INFO @ Sat, 03 Apr 2021 07:58:26: #2 number of paired peaks: 160 WARNING @ Sat, 03 Apr 2021 07:58:26: Fewer paired peaks (160) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 160 pairs to build model! INFO @ Sat, 03 Apr 2021 07:58:26: start model_add_line... INFO @ Sat, 03 Apr 2021 07:58:26: start X-correlation... INFO @ Sat, 03 Apr 2021 07:58:26: end of X-cor INFO @ Sat, 03 Apr 2021 07:58:26: #2 finished! INFO @ Sat, 03 Apr 2021 07:58:26: #2 predicted fragment length is 100 bps INFO @ Sat, 03 Apr 2021 07:58:26: #2 alternative fragment length(s) may be 4,100 bps INFO @ Sat, 03 Apr 2021 07:58:26: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.05_model.r WARNING @ Sat, 03 Apr 2021 07:58:26: #2 Since the d (100) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:58:26: #2 You may need to consider one of the other alternative d(s): 4,100 WARNING @ Sat, 03 Apr 2021 07:58:26: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:58:26: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:58:26: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:58:28: 51000000 INFO @ Sat, 03 Apr 2021 07:58:33: 52000000 INFO @ Sat, 03 Apr 2021 07:58:36: 52000000 INFO @ Sat, 03 Apr 2021 07:58:41: 53000000 INFO @ Sat, 03 Apr 2021 07:58:43: 53000000 INFO @ Sat, 03 Apr 2021 07:58:49: 54000000 INFO @ Sat, 03 Apr 2021 07:58:50: 54000000 INFO @ Sat, 03 Apr 2021 07:58:57: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:58:57: 55000000 INFO @ Sat, 03 Apr 2021 07:58:58: 55000000 INFO @ Sat, 03 Apr 2021 07:59:05: 56000000 INFO @ Sat, 03 Apr 2021 07:59:05: 56000000 INFO @ Sat, 03 Apr 2021 07:59:13: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.05_peaks.xls INFO @ Sat, 03 Apr 2021 07:59:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:59:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.05_summits.bed INFO @ Sat, 03 Apr 2021 07:59:13: Done! INFO @ Sat, 03 Apr 2021 07:59:13: 57000000 INFO @ Sat, 03 Apr 2021 07:59:13: 57000000 pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (8472 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:59:20: 58000000 INFO @ Sat, 03 Apr 2021 07:59:21: 58000000 INFO @ Sat, 03 Apr 2021 07:59:27: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 07:59:27: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 07:59:27: #1 total tags in treatment: 24213865 INFO @ Sat, 03 Apr 2021 07:59:27: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:59:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:59:28: #1 tag size is determined as 76 bps INFO @ Sat, 03 Apr 2021 07:59:28: #1 tag size = 76 INFO @ Sat, 03 Apr 2021 07:59:28: #1 total tags in treatment: 24213865 INFO @ Sat, 03 Apr 2021 07:59:28: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:59:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:59:28: #1 tags after filtering in treatment: 16701370 INFO @ Sat, 03 Apr 2021 07:59:28: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 03 Apr 2021 07:59:28: #1 finished! INFO @ Sat, 03 Apr 2021 07:59:28: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:59:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:59:28: #1 tags after filtering in treatment: 16701370 INFO @ Sat, 03 Apr 2021 07:59:28: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 03 Apr 2021 07:59:28: #1 finished! INFO @ Sat, 03 Apr 2021 07:59:28: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:59:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:59:29: #2 number of paired peaks: 160 WARNING @ Sat, 03 Apr 2021 07:59:29: Fewer paired peaks (160) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 160 pairs to build model! INFO @ Sat, 03 Apr 2021 07:59:29: start model_add_line... INFO @ Sat, 03 Apr 2021 07:59:29: start X-correlation... INFO @ Sat, 03 Apr 2021 07:59:29: end of X-cor INFO @ Sat, 03 Apr 2021 07:59:29: #2 finished! INFO @ Sat, 03 Apr 2021 07:59:29: #2 predicted fragment length is 100 bps INFO @ Sat, 03 Apr 2021 07:59:29: #2 alternative fragment length(s) may be 4,100 bps INFO @ Sat, 03 Apr 2021 07:59:29: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.20_model.r WARNING @ Sat, 03 Apr 2021 07:59:29: #2 Since the d (100) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:59:29: #2 You may need to consider one of the other alternative d(s): 4,100 WARNING @ Sat, 03 Apr 2021 07:59:29: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:59:29: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:59:29: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:59:29: #2 number of paired peaks: 160 WARNING @ Sat, 03 Apr 2021 07:59:29: Fewer paired peaks (160) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 160 pairs to build model! INFO @ Sat, 03 Apr 2021 07:59:29: start model_add_line... INFO @ Sat, 03 Apr 2021 07:59:30: start X-correlation... INFO @ Sat, 03 Apr 2021 07:59:30: end of X-cor INFO @ Sat, 03 Apr 2021 07:59:30: #2 finished! INFO @ Sat, 03 Apr 2021 07:59:30: #2 predicted fragment length is 100 bps INFO @ Sat, 03 Apr 2021 07:59:30: #2 alternative fragment length(s) may be 4,100 bps INFO @ Sat, 03 Apr 2021 07:59:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.10_model.r WARNING @ Sat, 03 Apr 2021 07:59:30: #2 Since the d (100) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:59:30: #2 You may need to consider one of the other alternative d(s): 4,100 WARNING @ Sat, 03 Apr 2021 07:59:30: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:59:30: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:59:30: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:59:58: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:00:00: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 08:00:13: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.20_peaks.xls INFO @ Sat, 03 Apr 2021 08:00:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:00:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.20_summits.bed INFO @ Sat, 03 Apr 2021 08:00:13: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (1764 records, 4 fields): 16 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 08:00:15: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.10_peaks.xls INFO @ Sat, 03 Apr 2021 08:00:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 08:00:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX3029130/SRX3029130.10_summits.bed INFO @ Sat, 03 Apr 2021 08:00:15: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (4663 records, 4 fields): 7 millis CompletedMACS2peakCalling