Job ID = 6626343 SRX = SRX3008771 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 44815642 spots for SRR5831453/SRR5831453.sra Written 44815642 spots for SRR5831453/SRR5831453.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626599 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:56 44815642 reads; of these: 44815642 (100.00%) were unpaired; of these: 454116 (1.01%) aligned 0 times 37670279 (84.06%) aligned exactly 1 time 6691247 (14.93%) aligned >1 times 98.99% overall alignment rate Time searching: 00:10:56 Overall time: 00:10:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8399722 / 44361526 = 0.1893 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:17:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:17:01: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:17:01: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:17:07: 1000000 INFO @ Tue, 14 Jul 2020 07:17:13: 2000000 INFO @ Tue, 14 Jul 2020 07:17:19: 3000000 INFO @ Tue, 14 Jul 2020 07:17:25: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:17:30: 5000000 INFO @ Tue, 14 Jul 2020 07:17:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:17:31: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:17:31: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:17:36: 6000000 INFO @ Tue, 14 Jul 2020 07:17:37: 1000000 INFO @ Tue, 14 Jul 2020 07:17:42: 7000000 INFO @ Tue, 14 Jul 2020 07:17:43: 2000000 INFO @ Tue, 14 Jul 2020 07:17:48: 8000000 INFO @ Tue, 14 Jul 2020 07:17:48: 3000000 INFO @ Tue, 14 Jul 2020 07:17:54: 9000000 INFO @ Tue, 14 Jul 2020 07:17:54: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:18:00: 5000000 INFO @ Tue, 14 Jul 2020 07:18:00: 10000000 INFO @ Tue, 14 Jul 2020 07:18:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:18:01: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:18:01: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:18:06: 6000000 INFO @ Tue, 14 Jul 2020 07:18:07: 11000000 INFO @ Tue, 14 Jul 2020 07:18:07: 1000000 INFO @ Tue, 14 Jul 2020 07:18:12: 7000000 INFO @ Tue, 14 Jul 2020 07:18:13: 2000000 INFO @ Tue, 14 Jul 2020 07:18:13: 12000000 INFO @ Tue, 14 Jul 2020 07:18:18: 8000000 INFO @ Tue, 14 Jul 2020 07:18:19: 3000000 INFO @ Tue, 14 Jul 2020 07:18:19: 13000000 INFO @ Tue, 14 Jul 2020 07:18:24: 9000000 INFO @ Tue, 14 Jul 2020 07:18:24: 4000000 INFO @ Tue, 14 Jul 2020 07:18:27: 14000000 INFO @ Tue, 14 Jul 2020 07:18:30: 10000000 INFO @ Tue, 14 Jul 2020 07:18:30: 5000000 INFO @ Tue, 14 Jul 2020 07:18:34: 15000000 INFO @ Tue, 14 Jul 2020 07:18:36: 11000000 INFO @ Tue, 14 Jul 2020 07:18:36: 6000000 INFO @ Tue, 14 Jul 2020 07:18:40: 16000000 INFO @ Tue, 14 Jul 2020 07:18:42: 7000000 INFO @ Tue, 14 Jul 2020 07:18:42: 12000000 INFO @ Tue, 14 Jul 2020 07:18:46: 17000000 INFO @ Tue, 14 Jul 2020 07:18:48: 8000000 INFO @ Tue, 14 Jul 2020 07:18:48: 13000000 INFO @ Tue, 14 Jul 2020 07:18:52: 18000000 INFO @ Tue, 14 Jul 2020 07:18:54: 9000000 INFO @ Tue, 14 Jul 2020 07:18:54: 14000000 INFO @ Tue, 14 Jul 2020 07:18:58: 19000000 INFO @ Tue, 14 Jul 2020 07:19:00: 10000000 INFO @ Tue, 14 Jul 2020 07:19:00: 15000000 INFO @ Tue, 14 Jul 2020 07:19:04: 20000000 INFO @ Tue, 14 Jul 2020 07:19:06: 11000000 INFO @ Tue, 14 Jul 2020 07:19:07: 16000000 INFO @ Tue, 14 Jul 2020 07:19:10: 21000000 INFO @ Tue, 14 Jul 2020 07:19:12: 12000000 INFO @ Tue, 14 Jul 2020 07:19:13: 17000000 INFO @ Tue, 14 Jul 2020 07:19:16: 22000000 INFO @ Tue, 14 Jul 2020 07:19:18: 13000000 INFO @ Tue, 14 Jul 2020 07:19:19: 18000000 INFO @ Tue, 14 Jul 2020 07:19:22: 23000000 INFO @ Tue, 14 Jul 2020 07:19:23: 14000000 INFO @ Tue, 14 Jul 2020 07:19:25: 19000000 INFO @ Tue, 14 Jul 2020 07:19:28: 24000000 INFO @ Tue, 14 Jul 2020 07:19:29: 15000000 INFO @ Tue, 14 Jul 2020 07:19:31: 20000000 INFO @ Tue, 14 Jul 2020 07:19:34: 25000000 INFO @ Tue, 14 Jul 2020 07:19:35: 16000000 INFO @ Tue, 14 Jul 2020 07:19:37: 21000000 INFO @ Tue, 14 Jul 2020 07:19:39: 26000000 INFO @ Tue, 14 Jul 2020 07:19:41: 17000000 INFO @ Tue, 14 Jul 2020 07:19:43: 22000000 INFO @ Tue, 14 Jul 2020 07:19:46: 27000000 INFO @ Tue, 14 Jul 2020 07:19:47: 18000000 INFO @ Tue, 14 Jul 2020 07:19:49: 23000000 INFO @ Tue, 14 Jul 2020 07:19:51: 28000000 INFO @ Tue, 14 Jul 2020 07:19:52: 19000000 INFO @ Tue, 14 Jul 2020 07:19:55: 24000000 INFO @ Tue, 14 Jul 2020 07:19:57: 29000000 INFO @ Tue, 14 Jul 2020 07:19:58: 20000000 INFO @ Tue, 14 Jul 2020 07:20:01: 25000000 INFO @ Tue, 14 Jul 2020 07:20:03: 30000000 INFO @ Tue, 14 Jul 2020 07:20:04: 21000000 INFO @ Tue, 14 Jul 2020 07:20:07: 26000000 INFO @ Tue, 14 Jul 2020 07:20:09: 31000000 INFO @ Tue, 14 Jul 2020 07:20:10: 22000000 INFO @ Tue, 14 Jul 2020 07:20:13: 27000000 INFO @ Tue, 14 Jul 2020 07:20:15: 32000000 INFO @ Tue, 14 Jul 2020 07:20:15: 23000000 INFO @ Tue, 14 Jul 2020 07:20:18: 28000000 INFO @ Tue, 14 Jul 2020 07:20:20: 33000000 INFO @ Tue, 14 Jul 2020 07:20:21: 24000000 INFO @ Tue, 14 Jul 2020 07:20:24: 29000000 INFO @ Tue, 14 Jul 2020 07:20:26: 34000000 INFO @ Tue, 14 Jul 2020 07:20:27: 25000000 INFO @ Tue, 14 Jul 2020 07:20:29: 30000000 INFO @ Tue, 14 Jul 2020 07:20:32: 35000000 INFO @ Tue, 14 Jul 2020 07:20:33: 26000000 INFO @ Tue, 14 Jul 2020 07:20:35: 31000000 INFO @ Tue, 14 Jul 2020 07:20:38: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:20:38: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:20:38: #1 total tags in treatment: 35961804 INFO @ Tue, 14 Jul 2020 07:20:38: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:20:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:20:39: #1 tags after filtering in treatment: 35961804 INFO @ Tue, 14 Jul 2020 07:20:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:20:39: #1 finished! INFO @ Tue, 14 Jul 2020 07:20:39: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:20:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:20:39: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 07:20:40: 32000000 INFO @ Tue, 14 Jul 2020 07:20:41: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 07:20:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:20:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:20:45: 28000000 INFO @ Tue, 14 Jul 2020 07:20:46: 33000000 INFO @ Tue, 14 Jul 2020 07:20:50: 29000000 INFO @ Tue, 14 Jul 2020 07:20:51: 34000000 INFO @ Tue, 14 Jul 2020 07:20:56: 30000000 INFO @ Tue, 14 Jul 2020 07:20:57: 35000000 INFO @ Tue, 14 Jul 2020 07:21:01: 31000000 INFO @ Tue, 14 Jul 2020 07:21:02: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:21:02: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:21:02: #1 total tags in treatment: 35961804 INFO @ Tue, 14 Jul 2020 07:21:02: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:21:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:21:03: #1 tags after filtering in treatment: 35961804 INFO @ Tue, 14 Jul 2020 07:21:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:21:03: #1 finished! INFO @ Tue, 14 Jul 2020 07:21:03: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:21:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:21:05: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 07:21:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:21:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:21:07: 32000000 INFO @ Tue, 14 Jul 2020 07:21:12: 33000000 INFO @ Tue, 14 Jul 2020 07:21:18: 34000000 INFO @ Tue, 14 Jul 2020 07:21:23: 35000000 INFO @ Tue, 14 Jul 2020 07:21:28: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:21:28: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:21:28: #1 total tags in treatment: 35961804 INFO @ Tue, 14 Jul 2020 07:21:28: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:21:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:21:29: #1 tags after filtering in treatment: 35961804 INFO @ Tue, 14 Jul 2020 07:21:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:21:29: #1 finished! INFO @ Tue, 14 Jul 2020 07:21:29: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:21:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:21:31: #2 number of paired peaks: 0 WARNING @ Tue, 14 Jul 2020 07:21:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:21:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008771/SRX3008771.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。