Job ID = 6626342 SRX = SRX3008770 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 40235808 spots for SRR5831452/SRR5831452.sra Written 40235808 spots for SRR5831452/SRR5831452.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626594 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:43 40235808 reads; of these: 40235808 (100.00%) were unpaired; of these: 402024 (1.00%) aligned 0 times 33825409 (84.07%) aligned exactly 1 time 6008375 (14.93%) aligned >1 times 99.00% overall alignment rate Time searching: 00:09:43 Overall time: 00:09:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 6784194 / 39833784 = 0.1703 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:14:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:14:53: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:14:53: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:14:59: 1000000 INFO @ Tue, 14 Jul 2020 07:15:05: 2000000 INFO @ Tue, 14 Jul 2020 07:15:11: 3000000 INFO @ Tue, 14 Jul 2020 07:15:17: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:15:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:15:23: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:15:23: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:15:23: 5000000 INFO @ Tue, 14 Jul 2020 07:15:30: 6000000 INFO @ Tue, 14 Jul 2020 07:15:30: 1000000 INFO @ Tue, 14 Jul 2020 07:15:37: 7000000 INFO @ Tue, 14 Jul 2020 07:15:37: 2000000 INFO @ Tue, 14 Jul 2020 07:15:43: 8000000 INFO @ Tue, 14 Jul 2020 07:15:44: 3000000 INFO @ Tue, 14 Jul 2020 07:15:50: 9000000 INFO @ Tue, 14 Jul 2020 07:15:50: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:15:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:15:53: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:15:53: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:15:56: 10000000 INFO @ Tue, 14 Jul 2020 07:15:57: 5000000 INFO @ Tue, 14 Jul 2020 07:16:00: 1000000 INFO @ Tue, 14 Jul 2020 07:16:03: 11000000 INFO @ Tue, 14 Jul 2020 07:16:04: 6000000 INFO @ Tue, 14 Jul 2020 07:16:07: 2000000 INFO @ Tue, 14 Jul 2020 07:16:10: 12000000 INFO @ Tue, 14 Jul 2020 07:16:11: 7000000 INFO @ Tue, 14 Jul 2020 07:16:14: 3000000 INFO @ Tue, 14 Jul 2020 07:16:17: 13000000 INFO @ Tue, 14 Jul 2020 07:16:18: 8000000 INFO @ Tue, 14 Jul 2020 07:16:21: 4000000 INFO @ Tue, 14 Jul 2020 07:16:24: 14000000 INFO @ Tue, 14 Jul 2020 07:16:25: 9000000 INFO @ Tue, 14 Jul 2020 07:16:28: 5000000 INFO @ Tue, 14 Jul 2020 07:16:31: 15000000 INFO @ Tue, 14 Jul 2020 07:16:32: 10000000 INFO @ Tue, 14 Jul 2020 07:16:35: 6000000 INFO @ Tue, 14 Jul 2020 07:16:38: 16000000 INFO @ Tue, 14 Jul 2020 07:16:39: 11000000 INFO @ Tue, 14 Jul 2020 07:16:42: 7000000 INFO @ Tue, 14 Jul 2020 07:16:45: 17000000 INFO @ Tue, 14 Jul 2020 07:16:46: 12000000 INFO @ Tue, 14 Jul 2020 07:16:49: 8000000 INFO @ Tue, 14 Jul 2020 07:16:53: 18000000 INFO @ Tue, 14 Jul 2020 07:16:53: 13000000 INFO @ Tue, 14 Jul 2020 07:16:56: 9000000 INFO @ Tue, 14 Jul 2020 07:17:00: 19000000 INFO @ Tue, 14 Jul 2020 07:17:00: 14000000 INFO @ Tue, 14 Jul 2020 07:17:03: 10000000 INFO @ Tue, 14 Jul 2020 07:17:07: 20000000 INFO @ Tue, 14 Jul 2020 07:17:07: 15000000 INFO @ Tue, 14 Jul 2020 07:17:10: 11000000 INFO @ Tue, 14 Jul 2020 07:17:13: 21000000 INFO @ Tue, 14 Jul 2020 07:17:14: 16000000 INFO @ Tue, 14 Jul 2020 07:17:17: 12000000 INFO @ Tue, 14 Jul 2020 07:17:20: 22000000 INFO @ Tue, 14 Jul 2020 07:17:21: 17000000 INFO @ Tue, 14 Jul 2020 07:17:24: 13000000 INFO @ Tue, 14 Jul 2020 07:17:27: 23000000 INFO @ Tue, 14 Jul 2020 07:17:28: 18000000 INFO @ Tue, 14 Jul 2020 07:17:30: 14000000 INFO @ Tue, 14 Jul 2020 07:17:35: 24000000 INFO @ Tue, 14 Jul 2020 07:17:35: 19000000 INFO @ Tue, 14 Jul 2020 07:17:37: 15000000 INFO @ Tue, 14 Jul 2020 07:17:41: 20000000 INFO @ Tue, 14 Jul 2020 07:17:41: 25000000 INFO @ Tue, 14 Jul 2020 07:17:44: 16000000 INFO @ Tue, 14 Jul 2020 07:17:48: 21000000 INFO @ Tue, 14 Jul 2020 07:17:48: 26000000 INFO @ Tue, 14 Jul 2020 07:17:51: 17000000 INFO @ Tue, 14 Jul 2020 07:17:55: 27000000 INFO @ Tue, 14 Jul 2020 07:17:55: 22000000 INFO @ Tue, 14 Jul 2020 07:17:58: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 07:18:02: 28000000 INFO @ Tue, 14 Jul 2020 07:18:03: 23000000 INFO @ Tue, 14 Jul 2020 07:18:05: 19000000 INFO @ Tue, 14 Jul 2020 07:18:09: 29000000 INFO @ Tue, 14 Jul 2020 07:18:10: 24000000 INFO @ Tue, 14 Jul 2020 07:18:12: 20000000 INFO @ Tue, 14 Jul 2020 07:18:17: 30000000 INFO @ Tue, 14 Jul 2020 07:18:18: 25000000 INFO @ Tue, 14 Jul 2020 07:18:19: 21000000 INFO @ Tue, 14 Jul 2020 07:18:24: 31000000 INFO @ Tue, 14 Jul 2020 07:18:26: 26000000 INFO @ Tue, 14 Jul 2020 07:18:27: 22000000 INFO @ Tue, 14 Jul 2020 07:18:32: 32000000 INFO @ Tue, 14 Jul 2020 07:18:34: 27000000 INFO @ Tue, 14 Jul 2020 07:18:35: 23000000 INFO @ Tue, 14 Jul 2020 07:18:40: 33000000 INFO @ Tue, 14 Jul 2020 07:18:41: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:18:41: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:18:41: #1 total tags in treatment: 33049590 INFO @ Tue, 14 Jul 2020 07:18:41: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:18:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:18:41: #1 tags after filtering in treatment: 33049590 INFO @ Tue, 14 Jul 2020 07:18:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:18:41: #1 finished! INFO @ Tue, 14 Jul 2020 07:18:41: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:18:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:18:43: 28000000 INFO @ Tue, 14 Jul 2020 07:18:43: 24000000 INFO @ Tue, 14 Jul 2020 07:18:43: #2 number of paired peaks: 23 WARNING @ Tue, 14 Jul 2020 07:18:43: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:18:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:18:50: 25000000 INFO @ Tue, 14 Jul 2020 07:18:51: 29000000 INFO @ Tue, 14 Jul 2020 07:18:58: 26000000 INFO @ Tue, 14 Jul 2020 07:18:58: 30000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 07:19:05: 27000000 INFO @ Tue, 14 Jul 2020 07:19:05: 31000000 INFO @ Tue, 14 Jul 2020 07:19:12: 28000000 INFO @ Tue, 14 Jul 2020 07:19:12: 32000000 INFO @ Tue, 14 Jul 2020 07:19:19: 29000000 INFO @ Tue, 14 Jul 2020 07:19:19: 33000000 INFO @ Tue, 14 Jul 2020 07:19:20: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:19:20: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:19:20: #1 total tags in treatment: 33049590 INFO @ Tue, 14 Jul 2020 07:19:20: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:19:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:19:20: #1 tags after filtering in treatment: 33049590 INFO @ Tue, 14 Jul 2020 07:19:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:19:20: #1 finished! INFO @ Tue, 14 Jul 2020 07:19:20: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:19:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:19:22: #2 number of paired peaks: 23 WARNING @ Tue, 14 Jul 2020 07:19:22: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:19:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:19:26: 30000000 INFO @ Tue, 14 Jul 2020 07:19:32: 31000000 INFO @ Tue, 14 Jul 2020 07:19:39: 32000000 INFO @ Tue, 14 Jul 2020 07:19:45: 33000000 INFO @ Tue, 14 Jul 2020 07:19:46: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:19:46: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:19:46: #1 total tags in treatment: 33049590 INFO @ Tue, 14 Jul 2020 07:19:46: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:19:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:19:46: #1 tags after filtering in treatment: 33049590 INFO @ Tue, 14 Jul 2020 07:19:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:19:46: #1 finished! INFO @ Tue, 14 Jul 2020 07:19:46: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:19:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:19:48: #2 number of paired peaks: 23 WARNING @ Tue, 14 Jul 2020 07:19:48: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:19:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008770/SRX3008770.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling