Job ID = 6626341 SRX = SRX3008769 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 42913454 spots for SRR5831451/SRR5831451.sra Written 42913454 spots for SRR5831451/SRR5831451.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626591 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:16 42913454 reads; of these: 42913454 (100.00%) were unpaired; of these: 530212 (1.24%) aligned 0 times 35925116 (83.72%) aligned exactly 1 time 6458126 (15.05%) aligned >1 times 98.76% overall alignment rate Time searching: 00:09:16 Overall time: 00:09:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7521493 / 42383242 = 0.1775 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:15:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:15:04: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:15:04: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:15:10: 1000000 INFO @ Tue, 14 Jul 2020 07:15:17: 2000000 INFO @ Tue, 14 Jul 2020 07:15:23: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:15:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:15:26: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:15:26: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:15:29: 4000000 INFO @ Tue, 14 Jul 2020 07:15:33: 1000000 INFO @ Tue, 14 Jul 2020 07:15:36: 5000000 INFO @ Tue, 14 Jul 2020 07:15:38: 2000000 INFO @ Tue, 14 Jul 2020 07:15:43: 6000000 INFO @ Tue, 14 Jul 2020 07:15:44: 3000000 INFO @ Tue, 14 Jul 2020 07:15:49: 4000000 INFO @ Tue, 14 Jul 2020 07:15:49: 7000000 INFO @ Tue, 14 Jul 2020 07:15:54: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:15:56: 8000000 INFO @ Tue, 14 Jul 2020 07:15:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:15:56: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:15:56: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:16:00: 6000000 INFO @ Tue, 14 Jul 2020 07:16:02: 9000000 INFO @ Tue, 14 Jul 2020 07:16:03: 1000000 INFO @ Tue, 14 Jul 2020 07:16:05: 7000000 INFO @ Tue, 14 Jul 2020 07:16:08: 2000000 INFO @ Tue, 14 Jul 2020 07:16:09: 10000000 INFO @ Tue, 14 Jul 2020 07:16:10: 8000000 INFO @ Tue, 14 Jul 2020 07:16:14: 3000000 INFO @ Tue, 14 Jul 2020 07:16:16: 9000000 INFO @ Tue, 14 Jul 2020 07:16:16: 11000000 INFO @ Tue, 14 Jul 2020 07:16:19: 4000000 INFO @ Tue, 14 Jul 2020 07:16:21: 10000000 INFO @ Tue, 14 Jul 2020 07:16:23: 12000000 INFO @ Tue, 14 Jul 2020 07:16:25: 5000000 INFO @ Tue, 14 Jul 2020 07:16:27: 11000000 INFO @ Tue, 14 Jul 2020 07:16:29: 13000000 INFO @ Tue, 14 Jul 2020 07:16:31: 6000000 INFO @ Tue, 14 Jul 2020 07:16:32: 12000000 INFO @ Tue, 14 Jul 2020 07:16:36: 14000000 INFO @ Tue, 14 Jul 2020 07:16:36: 7000000 INFO @ Tue, 14 Jul 2020 07:16:38: 13000000 INFO @ Tue, 14 Jul 2020 07:16:41: 8000000 INFO @ Tue, 14 Jul 2020 07:16:42: 15000000 INFO @ Tue, 14 Jul 2020 07:16:43: 14000000 INFO @ Tue, 14 Jul 2020 07:16:47: 9000000 INFO @ Tue, 14 Jul 2020 07:16:47: 16000000 INFO @ Tue, 14 Jul 2020 07:16:48: 15000000 INFO @ Tue, 14 Jul 2020 07:16:53: 17000000 INFO @ Tue, 14 Jul 2020 07:16:53: 10000000 INFO @ Tue, 14 Jul 2020 07:16:54: 16000000 INFO @ Tue, 14 Jul 2020 07:16:58: 18000000 INFO @ Tue, 14 Jul 2020 07:16:58: 11000000 INFO @ Tue, 14 Jul 2020 07:16:59: 17000000 INFO @ Tue, 14 Jul 2020 07:17:03: 19000000 INFO @ Tue, 14 Jul 2020 07:17:04: 12000000 INFO @ Tue, 14 Jul 2020 07:17:04: 18000000 INFO @ Tue, 14 Jul 2020 07:17:09: 20000000 INFO @ Tue, 14 Jul 2020 07:17:09: 13000000 INFO @ Tue, 14 Jul 2020 07:17:10: 19000000 INFO @ Tue, 14 Jul 2020 07:17:14: 21000000 INFO @ Tue, 14 Jul 2020 07:17:14: 14000000 INFO @ Tue, 14 Jul 2020 07:17:15: 20000000 INFO @ Tue, 14 Jul 2020 07:17:19: 22000000 INFO @ Tue, 14 Jul 2020 07:17:20: 15000000 INFO @ Tue, 14 Jul 2020 07:17:20: 21000000 INFO @ Tue, 14 Jul 2020 07:17:25: 23000000 INFO @ Tue, 14 Jul 2020 07:17:26: 16000000 INFO @ Tue, 14 Jul 2020 07:17:26: 22000000 INFO @ Tue, 14 Jul 2020 07:17:30: 24000000 INFO @ Tue, 14 Jul 2020 07:17:31: 17000000 INFO @ Tue, 14 Jul 2020 07:17:32: 23000000 INFO @ Tue, 14 Jul 2020 07:17:35: 25000000 INFO @ Tue, 14 Jul 2020 07:17:36: 18000000 INFO @ Tue, 14 Jul 2020 07:17:37: 24000000 INFO @ Tue, 14 Jul 2020 07:17:41: 26000000 INFO @ Tue, 14 Jul 2020 07:17:42: 19000000 INFO @ Tue, 14 Jul 2020 07:17:43: 25000000 INFO @ Tue, 14 Jul 2020 07:17:46: 27000000 INFO @ Tue, 14 Jul 2020 07:17:47: 20000000 INFO @ Tue, 14 Jul 2020 07:17:49: 26000000 INFO @ Tue, 14 Jul 2020 07:17:51: 28000000 INFO @ Tue, 14 Jul 2020 07:17:52: 21000000 INFO @ Tue, 14 Jul 2020 07:17:54: 27000000 INFO @ Tue, 14 Jul 2020 07:17:57: 29000000 INFO @ Tue, 14 Jul 2020 07:17:58: 22000000 INFO @ Tue, 14 Jul 2020 07:17:59: 28000000 INFO @ Tue, 14 Jul 2020 07:18:02: 30000000 INFO @ Tue, 14 Jul 2020 07:18:03: 23000000 INFO @ Tue, 14 Jul 2020 07:18:05: 29000000 INFO @ Tue, 14 Jul 2020 07:18:07: 31000000 INFO @ Tue, 14 Jul 2020 07:18:08: 24000000 INFO @ Tue, 14 Jul 2020 07:18:10: 30000000 INFO @ Tue, 14 Jul 2020 07:18:12: 32000000 INFO @ Tue, 14 Jul 2020 07:18:14: 25000000 INFO @ Tue, 14 Jul 2020 07:18:15: 31000000 INFO @ Tue, 14 Jul 2020 07:18:18: 33000000 INFO @ Tue, 14 Jul 2020 07:18:19: 26000000 INFO @ Tue, 14 Jul 2020 07:18:20: 32000000 INFO @ Tue, 14 Jul 2020 07:18:24: 34000000 INFO @ Tue, 14 Jul 2020 07:18:25: 27000000 INFO @ Tue, 14 Jul 2020 07:18:26: 33000000 INFO @ Tue, 14 Jul 2020 07:18:30: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:18:30: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:18:30: #1 total tags in treatment: 34861749 INFO @ Tue, 14 Jul 2020 07:18:30: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:18:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:18:30: 28000000 INFO @ Tue, 14 Jul 2020 07:18:30: #1 tags after filtering in treatment: 34861749 INFO @ Tue, 14 Jul 2020 07:18:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:18:30: #1 finished! INFO @ Tue, 14 Jul 2020 07:18:30: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:18:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:18:31: 34000000 INFO @ Tue, 14 Jul 2020 07:18:32: #2 number of paired peaks: 13 WARNING @ Tue, 14 Jul 2020 07:18:32: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:18:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:18:35: 29000000 INFO @ Tue, 14 Jul 2020 07:18:36: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:18:36: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:18:36: #1 total tags in treatment: 34861749 INFO @ Tue, 14 Jul 2020 07:18:36: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:18:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:18:37: #1 tags after filtering in treatment: 34861749 INFO @ Tue, 14 Jul 2020 07:18:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:18:37: #1 finished! INFO @ Tue, 14 Jul 2020 07:18:37: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:18:37: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 07:18:39: #2 number of paired peaks: 13 WARNING @ Tue, 14 Jul 2020 07:18:39: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:18:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:18:40: 30000000 INFO @ Tue, 14 Jul 2020 07:18:46: 31000000 INFO @ Tue, 14 Jul 2020 07:18:51: 32000000 INFO @ Tue, 14 Jul 2020 07:18:56: 33000000 INFO @ Tue, 14 Jul 2020 07:19:01: 34000000 INFO @ Tue, 14 Jul 2020 07:19:05: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:19:05: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:19:05: #1 total tags in treatment: 34861749 INFO @ Tue, 14 Jul 2020 07:19:05: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:19:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:19:06: #1 tags after filtering in treatment: 34861749 INFO @ Tue, 14 Jul 2020 07:19:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:19:06: #1 finished! INFO @ Tue, 14 Jul 2020 07:19:06: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:19:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:19:08: #2 number of paired peaks: 13 WARNING @ Tue, 14 Jul 2020 07:19:08: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:19:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008769/SRX3008769.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。