Job ID = 6626340 SRX = SRX3008768 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 42721018 spots for SRR5831450/SRR5831450.sra Written 42721018 spots for SRR5831450/SRR5831450.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626598 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:47 42721018 reads; of these: 42721018 (100.00%) were unpaired; of these: 522628 (1.22%) aligned 0 times 34419104 (80.57%) aligned exactly 1 time 7779286 (18.21%) aligned >1 times 98.78% overall alignment rate Time searching: 00:10:47 Overall time: 00:10:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7898718 / 42198390 = 0.1872 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:16:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:16:25: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:16:25: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:16:31: 1000000 INFO @ Tue, 14 Jul 2020 07:16:37: 2000000 INFO @ Tue, 14 Jul 2020 07:16:42: 3000000 INFO @ Tue, 14 Jul 2020 07:16:48: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:16:54: 5000000 INFO @ Tue, 14 Jul 2020 07:16:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:16:55: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:16:55: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:17:00: 6000000 INFO @ Tue, 14 Jul 2020 07:17:02: 1000000 INFO @ Tue, 14 Jul 2020 07:17:07: 7000000 INFO @ Tue, 14 Jul 2020 07:17:09: 2000000 INFO @ Tue, 14 Jul 2020 07:17:13: 8000000 INFO @ Tue, 14 Jul 2020 07:17:15: 3000000 INFO @ Tue, 14 Jul 2020 07:17:20: 9000000 INFO @ Tue, 14 Jul 2020 07:17:22: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:17:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:17:25: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:17:25: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:17:27: 10000000 INFO @ Tue, 14 Jul 2020 07:17:29: 5000000 INFO @ Tue, 14 Jul 2020 07:17:34: 1000000 INFO @ Tue, 14 Jul 2020 07:17:34: 11000000 INFO @ Tue, 14 Jul 2020 07:17:37: 6000000 INFO @ Tue, 14 Jul 2020 07:17:42: 12000000 INFO @ Tue, 14 Jul 2020 07:17:42: 2000000 INFO @ Tue, 14 Jul 2020 07:17:44: 7000000 INFO @ Tue, 14 Jul 2020 07:17:49: 13000000 INFO @ Tue, 14 Jul 2020 07:17:50: 3000000 INFO @ Tue, 14 Jul 2020 07:17:52: 8000000 INFO @ Tue, 14 Jul 2020 07:17:57: 14000000 INFO @ Tue, 14 Jul 2020 07:17:58: 4000000 INFO @ Tue, 14 Jul 2020 07:17:59: 9000000 INFO @ Tue, 14 Jul 2020 07:18:04: 15000000 INFO @ Tue, 14 Jul 2020 07:18:07: 5000000 INFO @ Tue, 14 Jul 2020 07:18:07: 10000000 INFO @ Tue, 14 Jul 2020 07:18:12: 16000000 INFO @ Tue, 14 Jul 2020 07:18:15: 11000000 INFO @ Tue, 14 Jul 2020 07:18:15: 6000000 INFO @ Tue, 14 Jul 2020 07:18:19: 17000000 INFO @ Tue, 14 Jul 2020 07:18:22: 12000000 INFO @ Tue, 14 Jul 2020 07:18:24: 7000000 INFO @ Tue, 14 Jul 2020 07:18:27: 18000000 INFO @ Tue, 14 Jul 2020 07:18:30: 13000000 INFO @ Tue, 14 Jul 2020 07:18:32: 8000000 INFO @ Tue, 14 Jul 2020 07:18:35: 19000000 INFO @ Tue, 14 Jul 2020 07:18:38: 14000000 INFO @ Tue, 14 Jul 2020 07:18:40: 9000000 INFO @ Tue, 14 Jul 2020 07:18:43: 20000000 INFO @ Tue, 14 Jul 2020 07:18:46: 15000000 INFO @ Tue, 14 Jul 2020 07:18:50: 21000000 INFO @ Tue, 14 Jul 2020 07:18:51: 10000000 INFO @ Tue, 14 Jul 2020 07:18:54: 16000000 INFO @ Tue, 14 Jul 2020 07:18:58: 22000000 INFO @ Tue, 14 Jul 2020 07:19:01: 17000000 INFO @ Tue, 14 Jul 2020 07:19:03: 11000000 INFO @ Tue, 14 Jul 2020 07:19:05: 23000000 INFO @ Tue, 14 Jul 2020 07:19:09: 18000000 INFO @ Tue, 14 Jul 2020 07:19:13: 24000000 INFO @ Tue, 14 Jul 2020 07:19:15: 12000000 INFO @ Tue, 14 Jul 2020 07:19:16: 19000000 INFO @ Tue, 14 Jul 2020 07:19:20: 25000000 INFO @ Tue, 14 Jul 2020 07:19:24: 20000000 INFO @ Tue, 14 Jul 2020 07:19:27: 13000000 INFO @ Tue, 14 Jul 2020 07:19:28: 26000000 INFO @ Tue, 14 Jul 2020 07:19:31: 21000000 INFO @ Tue, 14 Jul 2020 07:19:35: 27000000 INFO @ Tue, 14 Jul 2020 07:19:38: 14000000 INFO @ Tue, 14 Jul 2020 07:19:39: 22000000 INFO @ Tue, 14 Jul 2020 07:19:43: 28000000 INFO @ Tue, 14 Jul 2020 07:19:46: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 07:19:49: 15000000 INFO @ Tue, 14 Jul 2020 07:19:50: 29000000 INFO @ Tue, 14 Jul 2020 07:19:54: 24000000 INFO @ Tue, 14 Jul 2020 07:19:58: 30000000 INFO @ Tue, 14 Jul 2020 07:20:00: 16000000 INFO @ Tue, 14 Jul 2020 07:20:02: 25000000 INFO @ Tue, 14 Jul 2020 07:20:05: 31000000 INFO @ Tue, 14 Jul 2020 07:20:08: 17000000 INFO @ Tue, 14 Jul 2020 07:20:09: 26000000 INFO @ Tue, 14 Jul 2020 07:20:13: 32000000 INFO @ Tue, 14 Jul 2020 07:20:16: 18000000 INFO @ Tue, 14 Jul 2020 07:20:17: 27000000 INFO @ Tue, 14 Jul 2020 07:20:21: 33000000 INFO @ Tue, 14 Jul 2020 07:20:24: 19000000 INFO @ Tue, 14 Jul 2020 07:20:25: 28000000 INFO @ Tue, 14 Jul 2020 07:20:29: 34000000 INFO @ Tue, 14 Jul 2020 07:20:31: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:20:31: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:20:31: #1 total tags in treatment: 34299672 INFO @ Tue, 14 Jul 2020 07:20:31: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:20:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:20:32: #1 tags after filtering in treatment: 34299672 INFO @ Tue, 14 Jul 2020 07:20:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:20:32: #1 finished! INFO @ Tue, 14 Jul 2020 07:20:32: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:20:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:20:33: 29000000 INFO @ Tue, 14 Jul 2020 07:20:33: 20000000 INFO @ Tue, 14 Jul 2020 07:20:34: #2 number of paired peaks: 20 WARNING @ Tue, 14 Jul 2020 07:20:34: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:20:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:20:40: 30000000 INFO @ Tue, 14 Jul 2020 07:20:41: 21000000 INFO @ Tue, 14 Jul 2020 07:20:48: 31000000 INFO @ Tue, 14 Jul 2020 07:20:49: 22000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 07:20:55: 32000000 INFO @ Tue, 14 Jul 2020 07:20:57: 23000000 INFO @ Tue, 14 Jul 2020 07:21:03: 33000000 INFO @ Tue, 14 Jul 2020 07:21:05: 24000000 INFO @ Tue, 14 Jul 2020 07:21:10: 34000000 INFO @ Tue, 14 Jul 2020 07:21:13: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:21:13: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:21:13: #1 total tags in treatment: 34299672 INFO @ Tue, 14 Jul 2020 07:21:13: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:21:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:21:13: #1 tags after filtering in treatment: 34299672 INFO @ Tue, 14 Jul 2020 07:21:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:21:13: #1 finished! INFO @ Tue, 14 Jul 2020 07:21:13: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:21:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:21:14: 25000000 INFO @ Tue, 14 Jul 2020 07:21:15: #2 number of paired peaks: 20 WARNING @ Tue, 14 Jul 2020 07:21:15: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:21:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:21:21: 26000000 INFO @ Tue, 14 Jul 2020 07:21:29: 27000000 INFO @ Tue, 14 Jul 2020 07:21:36: 28000000 INFO @ Tue, 14 Jul 2020 07:21:43: 29000000 INFO @ Tue, 14 Jul 2020 07:21:50: 30000000 INFO @ Tue, 14 Jul 2020 07:21:58: 31000000 INFO @ Tue, 14 Jul 2020 07:22:05: 32000000 INFO @ Tue, 14 Jul 2020 07:22:12: 33000000 INFO @ Tue, 14 Jul 2020 07:22:19: 34000000 INFO @ Tue, 14 Jul 2020 07:22:21: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:22:21: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:22:21: #1 total tags in treatment: 34299672 INFO @ Tue, 14 Jul 2020 07:22:21: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:22:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:22:22: #1 tags after filtering in treatment: 34299672 INFO @ Tue, 14 Jul 2020 07:22:22: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:22:22: #1 finished! INFO @ Tue, 14 Jul 2020 07:22:22: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:22:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:22:24: #2 number of paired peaks: 20 WARNING @ Tue, 14 Jul 2020 07:22:24: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:22:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008768/SRX3008768.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling