Job ID = 6626337 SRX = SRX3008765 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 41940504 spots for SRR5831447/SRR5831447.sra Written 41940504 spots for SRR5831447/SRR5831447.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626588 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:09:06 41940504 reads; of these: 41940504 (100.00%) were unpaired; of these: 612686 (1.46%) aligned 0 times 33842775 (80.69%) aligned exactly 1 time 7485043 (17.85%) aligned >1 times 98.54% overall alignment rate Time searching: 00:09:07 Overall time: 00:09:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7440921 / 41327818 = 0.1800 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:14:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:14:13: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:14:13: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:14:20: 1000000 INFO @ Tue, 14 Jul 2020 07:14:27: 2000000 INFO @ Tue, 14 Jul 2020 07:14:33: 3000000 INFO @ Tue, 14 Jul 2020 07:14:40: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:14:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:14:43: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:14:43: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:14:47: 5000000 INFO @ Tue, 14 Jul 2020 07:14:50: 1000000 INFO @ Tue, 14 Jul 2020 07:14:54: 6000000 INFO @ Tue, 14 Jul 2020 07:14:57: 2000000 INFO @ Tue, 14 Jul 2020 07:15:00: 7000000 INFO @ Tue, 14 Jul 2020 07:15:04: 3000000 INFO @ Tue, 14 Jul 2020 07:15:07: 8000000 INFO @ Tue, 14 Jul 2020 07:15:11: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:15:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:15:14: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:15:14: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:15:14: 9000000 INFO @ Tue, 14 Jul 2020 07:15:19: 5000000 INFO @ Tue, 14 Jul 2020 07:15:20: 1000000 INFO @ Tue, 14 Jul 2020 07:15:21: 10000000 INFO @ Tue, 14 Jul 2020 07:15:26: 6000000 INFO @ Tue, 14 Jul 2020 07:15:27: 2000000 INFO @ Tue, 14 Jul 2020 07:15:29: 11000000 INFO @ Tue, 14 Jul 2020 07:15:33: 7000000 INFO @ Tue, 14 Jul 2020 07:15:34: 3000000 INFO @ Tue, 14 Jul 2020 07:15:36: 12000000 INFO @ Tue, 14 Jul 2020 07:15:40: 8000000 INFO @ Tue, 14 Jul 2020 07:15:40: 4000000 INFO @ Tue, 14 Jul 2020 07:15:43: 13000000 INFO @ Tue, 14 Jul 2020 07:15:47: 9000000 INFO @ Tue, 14 Jul 2020 07:15:47: 5000000 INFO @ Tue, 14 Jul 2020 07:15:50: 14000000 INFO @ Tue, 14 Jul 2020 07:15:54: 6000000 INFO @ Tue, 14 Jul 2020 07:15:54: 10000000 INFO @ Tue, 14 Jul 2020 07:15:57: 15000000 INFO @ Tue, 14 Jul 2020 07:16:00: 7000000 INFO @ Tue, 14 Jul 2020 07:16:01: 11000000 INFO @ Tue, 14 Jul 2020 07:16:04: 16000000 INFO @ Tue, 14 Jul 2020 07:16:06: 8000000 INFO @ Tue, 14 Jul 2020 07:16:08: 12000000 INFO @ Tue, 14 Jul 2020 07:16:11: 17000000 INFO @ Tue, 14 Jul 2020 07:16:13: 9000000 INFO @ Tue, 14 Jul 2020 07:16:15: 13000000 INFO @ Tue, 14 Jul 2020 07:16:18: 18000000 INFO @ Tue, 14 Jul 2020 07:16:19: 10000000 INFO @ Tue, 14 Jul 2020 07:16:22: 14000000 INFO @ Tue, 14 Jul 2020 07:16:25: 19000000 INFO @ Tue, 14 Jul 2020 07:16:26: 11000000 INFO @ Tue, 14 Jul 2020 07:16:30: 15000000 INFO @ Tue, 14 Jul 2020 07:16:32: 20000000 INFO @ Tue, 14 Jul 2020 07:16:32: 12000000 INFO @ Tue, 14 Jul 2020 07:16:37: 16000000 INFO @ Tue, 14 Jul 2020 07:16:39: 13000000 INFO @ Tue, 14 Jul 2020 07:16:39: 21000000 INFO @ Tue, 14 Jul 2020 07:16:44: 17000000 INFO @ Tue, 14 Jul 2020 07:16:45: 14000000 INFO @ Tue, 14 Jul 2020 07:16:46: 22000000 INFO @ Tue, 14 Jul 2020 07:16:51: 18000000 INFO @ Tue, 14 Jul 2020 07:16:52: 15000000 INFO @ Tue, 14 Jul 2020 07:16:53: 23000000 INFO @ Tue, 14 Jul 2020 07:16:58: 19000000 INFO @ Tue, 14 Jul 2020 07:16:58: 16000000 INFO @ Tue, 14 Jul 2020 07:17:01: 24000000 INFO @ Tue, 14 Jul 2020 07:17:05: 17000000 INFO @ Tue, 14 Jul 2020 07:17:05: 20000000 INFO @ Tue, 14 Jul 2020 07:17:08: 25000000 INFO @ Tue, 14 Jul 2020 07:17:11: 18000000 INFO @ Tue, 14 Jul 2020 07:17:12: 21000000 INFO @ Tue, 14 Jul 2020 07:17:15: 26000000 INFO @ Tue, 14 Jul 2020 07:17:17: 19000000 INFO @ Tue, 14 Jul 2020 07:17:19: 22000000 INFO @ Tue, 14 Jul 2020 07:17:22: 27000000 INFO @ Tue, 14 Jul 2020 07:17:24: 20000000 INFO @ Tue, 14 Jul 2020 07:17:26: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 07:17:29: 28000000 INFO @ Tue, 14 Jul 2020 07:17:30: 21000000 INFO @ Tue, 14 Jul 2020 07:17:33: 24000000 INFO @ Tue, 14 Jul 2020 07:17:36: 29000000 INFO @ Tue, 14 Jul 2020 07:17:37: 22000000 INFO @ Tue, 14 Jul 2020 07:17:41: 25000000 INFO @ Tue, 14 Jul 2020 07:17:43: 30000000 INFO @ Tue, 14 Jul 2020 07:17:43: 23000000 INFO @ Tue, 14 Jul 2020 07:17:48: 26000000 INFO @ Tue, 14 Jul 2020 07:17:50: 24000000 INFO @ Tue, 14 Jul 2020 07:17:50: 31000000 INFO @ Tue, 14 Jul 2020 07:17:55: 27000000 INFO @ Tue, 14 Jul 2020 07:17:56: 25000000 INFO @ Tue, 14 Jul 2020 07:17:57: 32000000 INFO @ Tue, 14 Jul 2020 07:18:02: 28000000 INFO @ Tue, 14 Jul 2020 07:18:03: 26000000 INFO @ Tue, 14 Jul 2020 07:18:04: 33000000 INFO @ Tue, 14 Jul 2020 07:18:09: 27000000 INFO @ Tue, 14 Jul 2020 07:18:09: 29000000 INFO @ Tue, 14 Jul 2020 07:18:10: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:18:10: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:18:10: #1 total tags in treatment: 33886897 INFO @ Tue, 14 Jul 2020 07:18:10: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:18:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:18:11: #1 tags after filtering in treatment: 33886897 INFO @ Tue, 14 Jul 2020 07:18:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:18:11: #1 finished! INFO @ Tue, 14 Jul 2020 07:18:11: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:18:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:18:13: #2 number of paired peaks: 33 WARNING @ Tue, 14 Jul 2020 07:18:13: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:18:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:18:15: 28000000 INFO @ Tue, 14 Jul 2020 07:18:16: 30000000 INFO @ Tue, 14 Jul 2020 07:18:22: 29000000 INFO @ Tue, 14 Jul 2020 07:18:23: 31000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 07:18:28: 30000000 INFO @ Tue, 14 Jul 2020 07:18:30: 32000000 INFO @ Tue, 14 Jul 2020 07:18:34: 31000000 INFO @ Tue, 14 Jul 2020 07:18:37: 33000000 INFO @ Tue, 14 Jul 2020 07:18:40: 32000000 INFO @ Tue, 14 Jul 2020 07:18:43: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:18:43: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:18:43: #1 total tags in treatment: 33886897 INFO @ Tue, 14 Jul 2020 07:18:43: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:18:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:18:44: #1 tags after filtering in treatment: 33886897 INFO @ Tue, 14 Jul 2020 07:18:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:18:44: #1 finished! INFO @ Tue, 14 Jul 2020 07:18:44: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:18:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:18:46: #2 number of paired peaks: 33 WARNING @ Tue, 14 Jul 2020 07:18:46: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:18:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:18:46: 33000000 INFO @ Tue, 14 Jul 2020 07:18:52: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:18:52: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:18:52: #1 total tags in treatment: 33886897 INFO @ Tue, 14 Jul 2020 07:18:52: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:18:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:18:52: #1 tags after filtering in treatment: 33886897 INFO @ Tue, 14 Jul 2020 07:18:52: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:18:52: #1 finished! INFO @ Tue, 14 Jul 2020 07:18:52: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:18:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:18:54: #2 number of paired peaks: 33 WARNING @ Tue, 14 Jul 2020 07:18:54: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:18:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008765/SRX3008765.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling