Job ID = 6626334 SRX = SRX3008762 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 43398663 spots for SRR5831444/SRR5831444.sra Written 43398663 spots for SRR5831444/SRR5831444.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626592 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:35 43398663 reads; of these: 43398663 (100.00%) were unpaired; of these: 700561 (1.61%) aligned 0 times 35754451 (82.39%) aligned exactly 1 time 6943651 (16.00%) aligned >1 times 98.39% overall alignment rate Time searching: 00:09:35 Overall time: 00:09:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7319187 / 42698102 = 0.1714 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:14:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:14:50: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:14:50: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:14:55: 1000000 INFO @ Tue, 14 Jul 2020 07:15:00: 2000000 INFO @ Tue, 14 Jul 2020 07:15:05: 3000000 INFO @ Tue, 14 Jul 2020 07:15:09: 4000000 INFO @ Tue, 14 Jul 2020 07:15:14: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:15:19: 6000000 INFO @ Tue, 14 Jul 2020 07:15:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:15:20: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:15:20: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:15:24: 7000000 INFO @ Tue, 14 Jul 2020 07:15:25: 1000000 INFO @ Tue, 14 Jul 2020 07:15:29: 8000000 INFO @ Tue, 14 Jul 2020 07:15:30: 2000000 INFO @ Tue, 14 Jul 2020 07:15:34: 9000000 INFO @ Tue, 14 Jul 2020 07:15:35: 3000000 INFO @ Tue, 14 Jul 2020 07:15:40: 10000000 INFO @ Tue, 14 Jul 2020 07:15:40: 4000000 INFO @ Tue, 14 Jul 2020 07:15:45: 11000000 INFO @ Tue, 14 Jul 2020 07:15:46: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:15:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:15:50: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:15:50: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:15:50: 12000000 INFO @ Tue, 14 Jul 2020 07:15:51: 6000000 INFO @ Tue, 14 Jul 2020 07:15:56: 13000000 INFO @ Tue, 14 Jul 2020 07:15:56: 1000000 INFO @ Tue, 14 Jul 2020 07:15:56: 7000000 INFO @ Tue, 14 Jul 2020 07:16:01: 14000000 INFO @ Tue, 14 Jul 2020 07:16:02: 8000000 INFO @ Tue, 14 Jul 2020 07:16:03: 2000000 INFO @ Tue, 14 Jul 2020 07:16:06: 15000000 INFO @ Tue, 14 Jul 2020 07:16:08: 9000000 INFO @ Tue, 14 Jul 2020 07:16:09: 3000000 INFO @ Tue, 14 Jul 2020 07:16:12: 16000000 INFO @ Tue, 14 Jul 2020 07:16:13: 10000000 INFO @ Tue, 14 Jul 2020 07:16:15: 4000000 INFO @ Tue, 14 Jul 2020 07:16:18: 17000000 INFO @ Tue, 14 Jul 2020 07:16:19: 11000000 INFO @ Tue, 14 Jul 2020 07:16:22: 5000000 INFO @ Tue, 14 Jul 2020 07:16:23: 18000000 INFO @ Tue, 14 Jul 2020 07:16:24: 12000000 INFO @ Tue, 14 Jul 2020 07:16:28: 6000000 INFO @ Tue, 14 Jul 2020 07:16:29: 19000000 INFO @ Tue, 14 Jul 2020 07:16:30: 13000000 INFO @ Tue, 14 Jul 2020 07:16:34: 20000000 INFO @ Tue, 14 Jul 2020 07:16:34: 7000000 INFO @ Tue, 14 Jul 2020 07:16:35: 14000000 INFO @ Tue, 14 Jul 2020 07:16:40: 21000000 INFO @ Tue, 14 Jul 2020 07:16:41: 15000000 INFO @ Tue, 14 Jul 2020 07:16:41: 8000000 INFO @ Tue, 14 Jul 2020 07:16:45: 22000000 INFO @ Tue, 14 Jul 2020 07:16:46: 16000000 INFO @ Tue, 14 Jul 2020 07:16:47: 9000000 INFO @ Tue, 14 Jul 2020 07:16:51: 23000000 INFO @ Tue, 14 Jul 2020 07:16:52: 17000000 INFO @ Tue, 14 Jul 2020 07:16:54: 10000000 INFO @ Tue, 14 Jul 2020 07:16:56: 24000000 INFO @ Tue, 14 Jul 2020 07:16:58: 18000000 INFO @ Tue, 14 Jul 2020 07:17:00: 11000000 INFO @ Tue, 14 Jul 2020 07:17:02: 25000000 INFO @ Tue, 14 Jul 2020 07:17:03: 19000000 INFO @ Tue, 14 Jul 2020 07:17:06: 12000000 INFO @ Tue, 14 Jul 2020 07:17:07: 26000000 INFO @ Tue, 14 Jul 2020 07:17:09: 20000000 INFO @ Tue, 14 Jul 2020 07:17:13: 13000000 INFO @ Tue, 14 Jul 2020 07:17:13: 27000000 INFO @ Tue, 14 Jul 2020 07:17:14: 21000000 INFO @ Tue, 14 Jul 2020 07:17:19: 28000000 INFO @ Tue, 14 Jul 2020 07:17:19: 14000000 INFO @ Tue, 14 Jul 2020 07:17:20: 22000000 INFO @ Tue, 14 Jul 2020 07:17:24: 29000000 INFO @ Tue, 14 Jul 2020 07:17:25: 15000000 INFO @ Tue, 14 Jul 2020 07:17:25: 23000000 INFO @ Tue, 14 Jul 2020 07:17:30: 30000000 INFO @ Tue, 14 Jul 2020 07:17:31: 24000000 INFO @ Tue, 14 Jul 2020 07:17:31: 16000000 INFO @ Tue, 14 Jul 2020 07:17:35: 31000000 INFO @ Tue, 14 Jul 2020 07:17:37: 25000000 INFO @ Tue, 14 Jul 2020 07:17:38: 17000000 INFO @ Tue, 14 Jul 2020 07:17:40: 32000000 INFO @ Tue, 14 Jul 2020 07:17:42: 26000000 INFO @ Tue, 14 Jul 2020 07:17:44: 18000000 INFO @ Tue, 14 Jul 2020 07:17:46: 33000000 INFO @ Tue, 14 Jul 2020 07:17:48: 27000000 INFO @ Tue, 14 Jul 2020 07:17:50: 19000000 INFO @ Tue, 14 Jul 2020 07:17:52: 34000000 INFO @ Tue, 14 Jul 2020 07:17:53: 28000000 INFO @ Tue, 14 Jul 2020 07:17:56: 20000000 INFO @ Tue, 14 Jul 2020 07:17:57: 35000000 INFO @ Tue, 14 Jul 2020 07:17:59: 29000000 INFO @ Tue, 14 Jul 2020 07:18:00: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:18:00: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:18:00: #1 total tags in treatment: 35378915 INFO @ Tue, 14 Jul 2020 07:18:00: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:18:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:18:00: #1 tags after filtering in treatment: 35378915 INFO @ Tue, 14 Jul 2020 07:18:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:18:00: #1 finished! INFO @ Tue, 14 Jul 2020 07:18:00: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:18:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:18:02: #2 number of paired peaks: 37 WARNING @ Tue, 14 Jul 2020 07:18:02: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:18:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:18:03: 21000000 INFO @ Tue, 14 Jul 2020 07:18:04: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 07:18:09: 22000000 INFO @ Tue, 14 Jul 2020 07:18:10: 31000000 INFO @ Tue, 14 Jul 2020 07:18:15: 23000000 INFO @ Tue, 14 Jul 2020 07:18:15: 32000000 INFO @ Tue, 14 Jul 2020 07:18:21: 33000000 INFO @ Tue, 14 Jul 2020 07:18:22: 24000000 INFO @ Tue, 14 Jul 2020 07:18:27: 34000000 INFO @ Tue, 14 Jul 2020 07:18:28: 25000000 INFO @ Tue, 14 Jul 2020 07:18:33: 35000000 INFO @ Tue, 14 Jul 2020 07:18:34: 26000000 INFO @ Tue, 14 Jul 2020 07:18:35: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:18:35: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:18:35: #1 total tags in treatment: 35378915 INFO @ Tue, 14 Jul 2020 07:18:35: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:18:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:18:36: #1 tags after filtering in treatment: 35378915 INFO @ Tue, 14 Jul 2020 07:18:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:18:36: #1 finished! INFO @ Tue, 14 Jul 2020 07:18:36: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:18:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:18:38: #2 number of paired peaks: 37 WARNING @ Tue, 14 Jul 2020 07:18:38: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:18:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:18:40: 27000000 INFO @ Tue, 14 Jul 2020 07:18:46: 28000000 INFO @ Tue, 14 Jul 2020 07:18:52: 29000000 INFO @ Tue, 14 Jul 2020 07:18:58: 30000000 INFO @ Tue, 14 Jul 2020 07:19:04: 31000000 INFO @ Tue, 14 Jul 2020 07:19:10: 32000000 BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 07:19:16: 33000000 INFO @ Tue, 14 Jul 2020 07:19:22: 34000000 INFO @ Tue, 14 Jul 2020 07:19:28: 35000000 INFO @ Tue, 14 Jul 2020 07:19:31: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:19:31: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:19:31: #1 total tags in treatment: 35378915 INFO @ Tue, 14 Jul 2020 07:19:31: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:19:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:19:31: #1 tags after filtering in treatment: 35378915 INFO @ Tue, 14 Jul 2020 07:19:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:19:31: #1 finished! INFO @ Tue, 14 Jul 2020 07:19:31: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:19:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:19:33: #2 number of paired peaks: 37 WARNING @ Tue, 14 Jul 2020 07:19:33: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 14 Jul 2020 07:19:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX3008762/SRX3008762.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling