Job ID = 10845154 sra ファイルのダウンロード中... Completed: 1783058K bytes transferred in 112 seconds (130386K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 35206966 spots for /home/okishinya/chipatlas/results/ce10/SRX2973394/SRR5786975.sra Written 35206966 spots for /home/okishinya/chipatlas/results/ce10/SRX2973394/SRR5786975.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:06 35206966 reads; of these: 35206966 (100.00%) were unpaired; of these: 2311437 (6.57%) aligned 0 times 27522276 (78.17%) aligned exactly 1 time 5373253 (15.26%) aligned >1 times 93.43% overall alignment rate Time searching: 00:19:06 Overall time: 00:19:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5963412 / 32895529 = 0.1813 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 04 Jul 2018 09:59:39: # Command line: callpeak -t SRX2973394.bam -f BAM -g ce -n SRX2973394.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2973394.20 # format = BAM # ChIP-seq file = ['SRX2973394.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 04 Jul 2018 09:59:39: #1 read tag files... INFO @ Wed, 04 Jul 2018 09:59:39: #1 read treatment tags... INFO @ Wed, 04 Jul 2018 09:59:39: # Command line: callpeak -t SRX2973394.bam -f BAM -g ce -n SRX2973394.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2973394.05 # format = BAM # ChIP-seq file = ['SRX2973394.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 04 Jul 2018 09:59:39: #1 read tag files... INFO @ Wed, 04 Jul 2018 09:59:39: #1 read treatment tags... INFO @ Wed, 04 Jul 2018 09:59:39: # Command line: callpeak -t SRX2973394.bam -f BAM -g ce -n SRX2973394.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2973394.10 # format = BAM # ChIP-seq file = ['SRX2973394.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 04 Jul 2018 09:59:39: #1 read tag files... INFO @ Wed, 04 Jul 2018 09:59:39: #1 read treatment tags... INFO @ Wed, 04 Jul 2018 09:59:47: 1000000 INFO @ Wed, 04 Jul 2018 09:59:48: 1000000 INFO @ Wed, 04 Jul 2018 09:59:48: 1000000 INFO @ Wed, 04 Jul 2018 09:59:55: 2000000 INFO @ Wed, 04 Jul 2018 09:59:56: 2000000 INFO @ Wed, 04 Jul 2018 09:59:56: 2000000 INFO @ Wed, 04 Jul 2018 10:00:03: 3000000 INFO @ Wed, 04 Jul 2018 10:00:05: 3000000 INFO @ Wed, 04 Jul 2018 10:00:05: 3000000 INFO @ Wed, 04 Jul 2018 10:00:12: 4000000 INFO @ Wed, 04 Jul 2018 10:00:13: 4000000 INFO @ Wed, 04 Jul 2018 10:00:13: 4000000 INFO @ Wed, 04 Jul 2018 10:00:20: 5000000 INFO @ Wed, 04 Jul 2018 10:00:21: 5000000 INFO @ Wed, 04 Jul 2018 10:00:21: 5000000 INFO @ Wed, 04 Jul 2018 10:00:28: 6000000 INFO @ Wed, 04 Jul 2018 10:00:30: 6000000 INFO @ Wed, 04 Jul 2018 10:00:30: 6000000 INFO @ Wed, 04 Jul 2018 10:00:36: 7000000 INFO @ Wed, 04 Jul 2018 10:00:38: 7000000 INFO @ Wed, 04 Jul 2018 10:00:38: 7000000 INFO @ Wed, 04 Jul 2018 10:00:44: 8000000 INFO @ Wed, 04 Jul 2018 10:00:47: 8000000 INFO @ Wed, 04 Jul 2018 10:00:47: 8000000 INFO @ Wed, 04 Jul 2018 10:00:52: 9000000 INFO @ Wed, 04 Jul 2018 10:00:55: 9000000 INFO @ Wed, 04 Jul 2018 10:00:55: 9000000 INFO @ Wed, 04 Jul 2018 10:01:00: 10000000 INFO @ Wed, 04 Jul 2018 10:01:04: 10000000 INFO @ Wed, 04 Jul 2018 10:01:04: 10000000 INFO @ Wed, 04 Jul 2018 10:01:08: 11000000 INFO @ Wed, 04 Jul 2018 10:01:12: 11000000 INFO @ Wed, 04 Jul 2018 10:01:12: 11000000 INFO @ Wed, 04 Jul 2018 10:01:16: 12000000 INFO @ Wed, 04 Jul 2018 10:01:21: 12000000 INFO @ Wed, 04 Jul 2018 10:01:21: 12000000 INFO @ Wed, 04 Jul 2018 10:01:24: 13000000 INFO @ Wed, 04 Jul 2018 10:01:29: 13000000 INFO @ Wed, 04 Jul 2018 10:01:29: 13000000 INFO @ Wed, 04 Jul 2018 10:01:33: 14000000 INFO @ Wed, 04 Jul 2018 10:01:38: 14000000 INFO @ Wed, 04 Jul 2018 10:01:38: 14000000 INFO @ Wed, 04 Jul 2018 10:01:41: 15000000 INFO @ Wed, 04 Jul 2018 10:01:46: 15000000 INFO @ Wed, 04 Jul 2018 10:01:46: 15000000 INFO @ Wed, 04 Jul 2018 10:01:49: 16000000 INFO @ Wed, 04 Jul 2018 10:01:55: 16000000 INFO @ Wed, 04 Jul 2018 10:01:55: 16000000 INFO @ Wed, 04 Jul 2018 10:01:57: 17000000 INFO @ Wed, 04 Jul 2018 10:02:03: 17000000 INFO @ Wed, 04 Jul 2018 10:02:03: 17000000 INFO @ Wed, 04 Jul 2018 10:02:05: 18000000 INFO @ Wed, 04 Jul 2018 10:02:12: 18000000 INFO @ Wed, 04 Jul 2018 10:02:12: 18000000 INFO @ Wed, 04 Jul 2018 10:02:13: 19000000 INFO @ Wed, 04 Jul 2018 10:02:20: 19000000 INFO @ Wed, 04 Jul 2018 10:02:20: 19000000 INFO @ Wed, 04 Jul 2018 10:02:21: 20000000 INFO @ Wed, 04 Jul 2018 10:02:29: 20000000 INFO @ Wed, 04 Jul 2018 10:02:29: 20000000 INFO @ Wed, 04 Jul 2018 10:02:29: 21000000 INFO @ Wed, 04 Jul 2018 10:02:37: 21000000 INFO @ Wed, 04 Jul 2018 10:02:37: 21000000 INFO @ Wed, 04 Jul 2018 10:02:37: 22000000 INFO @ Wed, 04 Jul 2018 10:02:45: 23000000 INFO @ Wed, 04 Jul 2018 10:02:46: 22000000 INFO @ Wed, 04 Jul 2018 10:02:46: 22000000 INFO @ Wed, 04 Jul 2018 10:02:53: 24000000 INFO @ Wed, 04 Jul 2018 10:02:54: 23000000 INFO @ Wed, 04 Jul 2018 10:02:54: 23000000 INFO @ Wed, 04 Jul 2018 10:03:03: 25000000 INFO @ Wed, 04 Jul 2018 10:03:05: 24000000 INFO @ Wed, 04 Jul 2018 10:03:05: 24000000 INFO @ Wed, 04 Jul 2018 10:03:13: 26000000 INFO @ Wed, 04 Jul 2018 10:03:16: 25000000 INFO @ Wed, 04 Jul 2018 10:03:16: 25000000 INFO @ Wed, 04 Jul 2018 10:03:21: #1 tag size is determined as 101 bps INFO @ Wed, 04 Jul 2018 10:03:21: #1 tag size = 101 INFO @ Wed, 04 Jul 2018 10:03:21: #1 total tags in treatment: 26932117 INFO @ Wed, 04 Jul 2018 10:03:21: #1 user defined the maximum tags... INFO @ Wed, 04 Jul 2018 10:03:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 04 Jul 2018 10:03:22: #1 tags after filtering in treatment: 26932117 INFO @ Wed, 04 Jul 2018 10:03:22: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 04 Jul 2018 10:03:22: #1 finished! INFO @ Wed, 04 Jul 2018 10:03:22: #2 Build Peak Model... INFO @ Wed, 04 Jul 2018 10:03:22: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 04 Jul 2018 10:03:24: #2 number of paired peaks: 84 WARNING @ Wed, 04 Jul 2018 10:03:24: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 04 Jul 2018 10:03:24: Process for pairing-model is terminated! cat: SRX2973394.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2973394.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973394.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973394.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 04 Jul 2018 10:03:27: 26000000 INFO @ Wed, 04 Jul 2018 10:03:27: 26000000 INFO @ Wed, 04 Jul 2018 10:03:38: #1 tag size is determined as 101 bps INFO @ Wed, 04 Jul 2018 10:03:38: #1 tag size is determined as 101 bps INFO @ Wed, 04 Jul 2018 10:03:38: #1 tag size = 101 INFO @ Wed, 04 Jul 2018 10:03:38: #1 tag size = 101 INFO @ Wed, 04 Jul 2018 10:03:38: #1 total tags in treatment: 26932117 INFO @ Wed, 04 Jul 2018 10:03:38: #1 total tags in treatment: 26932117 INFO @ Wed, 04 Jul 2018 10:03:38: #1 user defined the maximum tags... INFO @ Wed, 04 Jul 2018 10:03:38: #1 user defined the maximum tags... INFO @ Wed, 04 Jul 2018 10:03:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 04 Jul 2018 10:03:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 04 Jul 2018 10:03:38: #1 tags after filtering in treatment: 26932117 INFO @ Wed, 04 Jul 2018 10:03:38: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 04 Jul 2018 10:03:38: #1 finished! INFO @ Wed, 04 Jul 2018 10:03:38: #2 Build Peak Model... INFO @ Wed, 04 Jul 2018 10:03:38: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 04 Jul 2018 10:03:38: #1 tags after filtering in treatment: 26932117 INFO @ Wed, 04 Jul 2018 10:03:38: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 04 Jul 2018 10:03:38: #1 finished! INFO @ Wed, 04 Jul 2018 10:03:38: #2 Build Peak Model... INFO @ Wed, 04 Jul 2018 10:03:38: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 04 Jul 2018 10:03:40: #2 number of paired peaks: 84 WARNING @ Wed, 04 Jul 2018 10:03:40: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 04 Jul 2018 10:03:40: Process for pairing-model is terminated! INFO @ Wed, 04 Jul 2018 10:03:40: #2 number of paired peaks: 84 WARNING @ Wed, 04 Jul 2018 10:03:40: Too few paired peaks (84) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 04 Jul 2018 10:03:40: Process for pairing-model is terminated! cat: SRX2973394.05_peaks.narrowPeakcat: SRX2973394.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません : そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2973394.05_model.r'rm: cannot remove `SRX2973394.20_model.r': そのようなファイルやディレクトリはありません : そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973394.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973394.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973394.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2973394.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。