Job ID = 2589619 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 6,189,031 reads read : 6,189,031 reads written : 6,189,031 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:54 6189031 reads; of these: 6189031 (100.00%) were unpaired; of these: 1022746 (16.53%) aligned 0 times 4362091 (70.48%) aligned exactly 1 time 804194 (12.99%) aligned >1 times 83.47% overall alignment rate Time searching: 00:00:54 Overall time: 00:00:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 360239 / 5166285 = 0.0697 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 18:08:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:08:19: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:08:19: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:08:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:08:19: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:08:19: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:08:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:08:20: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:08:20: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:08:27: 1000000 INFO @ Mon, 12 Aug 2019 18:08:28: 1000000 INFO @ Mon, 12 Aug 2019 18:08:30: 1000000 INFO @ Mon, 12 Aug 2019 18:08:35: 2000000 INFO @ Mon, 12 Aug 2019 18:08:38: 2000000 INFO @ Mon, 12 Aug 2019 18:08:40: 2000000 INFO @ Mon, 12 Aug 2019 18:08:43: 3000000 INFO @ Mon, 12 Aug 2019 18:08:49: 3000000 INFO @ Mon, 12 Aug 2019 18:08:51: 4000000 INFO @ Mon, 12 Aug 2019 18:08:51: 3000000 INFO @ Mon, 12 Aug 2019 18:08:57: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:08:57: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:08:57: #1 total tags in treatment: 4806046 INFO @ Mon, 12 Aug 2019 18:08:57: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:08:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:08:58: #1 tags after filtering in treatment: 4806046 INFO @ Mon, 12 Aug 2019 18:08:58: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:08:58: #1 finished! INFO @ Mon, 12 Aug 2019 18:08:58: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:08:58: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:08:58: #2 number of paired peaks: 350 WARNING @ Mon, 12 Aug 2019 18:08:58: Fewer paired peaks (350) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 350 pairs to build model! INFO @ Mon, 12 Aug 2019 18:08:58: start model_add_line... INFO @ Mon, 12 Aug 2019 18:08:58: start X-correlation... INFO @ Mon, 12 Aug 2019 18:08:58: end of X-cor INFO @ Mon, 12 Aug 2019 18:08:58: #2 finished! INFO @ Mon, 12 Aug 2019 18:08:58: #2 predicted fragment length is 30 bps INFO @ Mon, 12 Aug 2019 18:08:58: #2 alternative fragment length(s) may be 3,30,75,493,556 bps INFO @ Mon, 12 Aug 2019 18:08:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.10_model.r WARNING @ Mon, 12 Aug 2019 18:08:58: #2 Since the d (30) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:08:58: #2 You may need to consider one of the other alternative d(s): 3,30,75,493,556 WARNING @ Mon, 12 Aug 2019 18:08:58: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:08:58: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:08:58: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:09:00: 4000000 INFO @ Mon, 12 Aug 2019 18:09:02: 4000000 INFO @ Mon, 12 Aug 2019 18:09:08: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:09:08: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:09:08: #1 total tags in treatment: 4806046 INFO @ Mon, 12 Aug 2019 18:09:08: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:09:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:09:08: #1 tags after filtering in treatment: 4806046 INFO @ Mon, 12 Aug 2019 18:09:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:09:08: #1 finished! INFO @ Mon, 12 Aug 2019 18:09:08: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:09:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:09:08: #2 number of paired peaks: 350 WARNING @ Mon, 12 Aug 2019 18:09:08: Fewer paired peaks (350) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 350 pairs to build model! INFO @ Mon, 12 Aug 2019 18:09:08: start model_add_line... INFO @ Mon, 12 Aug 2019 18:09:08: start X-correlation... INFO @ Mon, 12 Aug 2019 18:09:08: end of X-cor INFO @ Mon, 12 Aug 2019 18:09:08: #2 finished! INFO @ Mon, 12 Aug 2019 18:09:08: #2 predicted fragment length is 30 bps INFO @ Mon, 12 Aug 2019 18:09:08: #2 alternative fragment length(s) may be 3,30,75,493,556 bps INFO @ Mon, 12 Aug 2019 18:09:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.05_model.r WARNING @ Mon, 12 Aug 2019 18:09:08: #2 Since the d (30) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:09:08: #2 You may need to consider one of the other alternative d(s): 3,30,75,493,556 WARNING @ Mon, 12 Aug 2019 18:09:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:09:08: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:09:08: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:09:09: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:09:09: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:09:09: #1 total tags in treatment: 4806046 INFO @ Mon, 12 Aug 2019 18:09:09: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:09:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:09:09: #1 tags after filtering in treatment: 4806046 INFO @ Mon, 12 Aug 2019 18:09:09: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:09:09: #1 finished! INFO @ Mon, 12 Aug 2019 18:09:09: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:09:09: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:09:10: #2 number of paired peaks: 350 WARNING @ Mon, 12 Aug 2019 18:09:10: Fewer paired peaks (350) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 350 pairs to build model! INFO @ Mon, 12 Aug 2019 18:09:10: start model_add_line... INFO @ Mon, 12 Aug 2019 18:09:10: start X-correlation... INFO @ Mon, 12 Aug 2019 18:09:10: end of X-cor INFO @ Mon, 12 Aug 2019 18:09:10: #2 finished! INFO @ Mon, 12 Aug 2019 18:09:10: #2 predicted fragment length is 30 bps INFO @ Mon, 12 Aug 2019 18:09:10: #2 alternative fragment length(s) may be 3,30,75,493,556 bps INFO @ Mon, 12 Aug 2019 18:09:10: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.20_model.r WARNING @ Mon, 12 Aug 2019 18:09:10: #2 Since the d (30) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:09:10: #2 You may need to consider one of the other alternative d(s): 3,30,75,493,556 WARNING @ Mon, 12 Aug 2019 18:09:10: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:09:10: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:09:10: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:09:12: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:09:19: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.10_peaks.xls INFO @ Mon, 12 Aug 2019 18:09:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:09:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.10_summits.bed INFO @ Mon, 12 Aug 2019 18:09:19: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (173 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:09:22: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:09:24: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:09:29: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.05_peaks.xls INFO @ Mon, 12 Aug 2019 18:09:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:09:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.05_summits.bed INFO @ Mon, 12 Aug 2019 18:09:29: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (358 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:09:30: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.20_peaks.xls INFO @ Mon, 12 Aug 2019 18:09:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:09:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX277058/SRX277058.20_summits.bed INFO @ Mon, 12 Aug 2019 18:09:31: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (61 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。