Job ID = 2589601 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 4,390,240 reads read : 4,390,240 reads written : 4,390,240 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:55 4390240 reads; of these: 4390240 (100.00%) were unpaired; of these: 33965 (0.77%) aligned 0 times 3687642 (84.00%) aligned exactly 1 time 668633 (15.23%) aligned >1 times 99.23% overall alignment rate Time searching: 00:00:55 Overall time: 00:00:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 292639 / 4356275 = 0.0672 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 18:05:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:05:22: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:05:22: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:05:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:05:23: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:05:23: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:05:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:05:24: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:05:24: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:05:29: 1000000 INFO @ Mon, 12 Aug 2019 18:05:29: 1000000 INFO @ Mon, 12 Aug 2019 18:05:30: 1000000 INFO @ Mon, 12 Aug 2019 18:05:36: 2000000 INFO @ Mon, 12 Aug 2019 18:05:37: 2000000 INFO @ Mon, 12 Aug 2019 18:05:37: 2000000 INFO @ Mon, 12 Aug 2019 18:05:42: 3000000 INFO @ Mon, 12 Aug 2019 18:05:43: 3000000 INFO @ Mon, 12 Aug 2019 18:05:44: 3000000 INFO @ Mon, 12 Aug 2019 18:05:48: 4000000 INFO @ Mon, 12 Aug 2019 18:05:49: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:05:49: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:05:49: #1 total tags in treatment: 4063636 INFO @ Mon, 12 Aug 2019 18:05:49: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:05:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:05:49: #1 tags after filtering in treatment: 4063636 INFO @ Mon, 12 Aug 2019 18:05:49: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:05:49: #1 finished! INFO @ Mon, 12 Aug 2019 18:05:49: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:05:49: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:05:49: 4000000 INFO @ Mon, 12 Aug 2019 18:05:49: #2 number of paired peaks: 338 WARNING @ Mon, 12 Aug 2019 18:05:49: Fewer paired peaks (338) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 338 pairs to build model! INFO @ Mon, 12 Aug 2019 18:05:49: start model_add_line... INFO @ Mon, 12 Aug 2019 18:05:49: start X-correlation... INFO @ Mon, 12 Aug 2019 18:05:49: end of X-cor INFO @ Mon, 12 Aug 2019 18:05:49: #2 finished! INFO @ Mon, 12 Aug 2019 18:05:49: #2 predicted fragment length is 33 bps INFO @ Mon, 12 Aug 2019 18:05:49: #2 alternative fragment length(s) may be 3,33,89,223,436,498,556,584 bps INFO @ Mon, 12 Aug 2019 18:05:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.10_model.r WARNING @ Mon, 12 Aug 2019 18:05:49: #2 Since the d (33) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:05:49: #2 You may need to consider one of the other alternative d(s): 3,33,89,223,436,498,556,584 WARNING @ Mon, 12 Aug 2019 18:05:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:05:49: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:05:49: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:05:49: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:05:49: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:05:49: #1 total tags in treatment: 4063636 INFO @ Mon, 12 Aug 2019 18:05:49: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:05:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:05:50: #1 tags after filtering in treatment: 4063636 INFO @ Mon, 12 Aug 2019 18:05:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:05:50: #1 finished! INFO @ Mon, 12 Aug 2019 18:05:50: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:05:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:05:50: #2 number of paired peaks: 338 WARNING @ Mon, 12 Aug 2019 18:05:50: Fewer paired peaks (338) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 338 pairs to build model! INFO @ Mon, 12 Aug 2019 18:05:50: start model_add_line... INFO @ Mon, 12 Aug 2019 18:05:50: start X-correlation... INFO @ Mon, 12 Aug 2019 18:05:50: end of X-cor INFO @ Mon, 12 Aug 2019 18:05:50: #2 finished! INFO @ Mon, 12 Aug 2019 18:05:50: #2 predicted fragment length is 33 bps INFO @ Mon, 12 Aug 2019 18:05:50: #2 alternative fragment length(s) may be 3,33,89,223,436,498,556,584 bps INFO @ Mon, 12 Aug 2019 18:05:50: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.20_model.r WARNING @ Mon, 12 Aug 2019 18:05:50: #2 Since the d (33) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:05:50: #2 You may need to consider one of the other alternative d(s): 3,33,89,223,436,498,556,584 WARNING @ Mon, 12 Aug 2019 18:05:50: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:05:50: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:05:50: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:05:51: 4000000 INFO @ Mon, 12 Aug 2019 18:05:51: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:05:51: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:05:51: #1 total tags in treatment: 4063636 INFO @ Mon, 12 Aug 2019 18:05:51: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:05:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:05:52: #1 tags after filtering in treatment: 4063636 INFO @ Mon, 12 Aug 2019 18:05:52: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:05:52: #1 finished! INFO @ Mon, 12 Aug 2019 18:05:52: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:05:52: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:05:52: #2 number of paired peaks: 338 WARNING @ Mon, 12 Aug 2019 18:05:52: Fewer paired peaks (338) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 338 pairs to build model! INFO @ Mon, 12 Aug 2019 18:05:52: start model_add_line... INFO @ Mon, 12 Aug 2019 18:05:52: start X-correlation... INFO @ Mon, 12 Aug 2019 18:05:52: end of X-cor INFO @ Mon, 12 Aug 2019 18:05:52: #2 finished! INFO @ Mon, 12 Aug 2019 18:05:52: #2 predicted fragment length is 33 bps INFO @ Mon, 12 Aug 2019 18:05:52: #2 alternative fragment length(s) may be 3,33,89,223,436,498,556,584 bps INFO @ Mon, 12 Aug 2019 18:05:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.05_model.r WARNING @ Mon, 12 Aug 2019 18:05:52: #2 Since the d (33) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:05:52: #2 You may need to consider one of the other alternative d(s): 3,33,89,223,436,498,556,584 WARNING @ Mon, 12 Aug 2019 18:05:52: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:05:52: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:05:52: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:06:01: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:06:02: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:06:04: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:06:07: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.10_peaks.xls INFO @ Mon, 12 Aug 2019 18:06:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:06:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.10_summits.bed INFO @ Mon, 12 Aug 2019 18:06:07: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (106 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:06:08: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.20_peaks.xls INFO @ Mon, 12 Aug 2019 18:06:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:06:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.20_summits.bed INFO @ Mon, 12 Aug 2019 18:06:08: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (19 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:06:10: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.05_peaks.xls INFO @ Mon, 12 Aug 2019 18:06:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:06:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX277034/SRX277034.05_summits.bed INFO @ Mon, 12 Aug 2019 18:06:10: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (286 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。