Job ID = 2589584 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 4,752,630 reads read : 4,752,630 reads written : 4,752,630 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:44 4752630 reads; of these: 4752630 (100.00%) were unpaired; of these: 568729 (11.97%) aligned 0 times 3495601 (73.55%) aligned exactly 1 time 688300 (14.48%) aligned >1 times 88.03% overall alignment rate Time searching: 00:00:44 Overall time: 00:00:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 208742 / 4183901 = 0.0499 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 18:02:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:02:56: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:02:56: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:02:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:02:57: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:02:57: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:02:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:02:58: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:02:58: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:03:04: 1000000 INFO @ Mon, 12 Aug 2019 18:03:06: 1000000 INFO @ Mon, 12 Aug 2019 18:03:06: 1000000 INFO @ Mon, 12 Aug 2019 18:03:12: 2000000 INFO @ Mon, 12 Aug 2019 18:03:14: 2000000 INFO @ Mon, 12 Aug 2019 18:03:16: 2000000 INFO @ Mon, 12 Aug 2019 18:03:20: 3000000 INFO @ Mon, 12 Aug 2019 18:03:22: 3000000 INFO @ Mon, 12 Aug 2019 18:03:25: 3000000 INFO @ Mon, 12 Aug 2019 18:03:27: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:03:27: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:03:27: #1 total tags in treatment: 3975159 INFO @ Mon, 12 Aug 2019 18:03:27: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:03:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:03:28: #1 tags after filtering in treatment: 3975159 INFO @ Mon, 12 Aug 2019 18:03:28: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:03:28: #1 finished! INFO @ Mon, 12 Aug 2019 18:03:28: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:03:28: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:03:28: #2 number of paired peaks: 403 WARNING @ Mon, 12 Aug 2019 18:03:28: Fewer paired peaks (403) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 403 pairs to build model! INFO @ Mon, 12 Aug 2019 18:03:28: start model_add_line... INFO @ Mon, 12 Aug 2019 18:03:28: start X-correlation... INFO @ Mon, 12 Aug 2019 18:03:28: end of X-cor INFO @ Mon, 12 Aug 2019 18:03:28: #2 finished! INFO @ Mon, 12 Aug 2019 18:03:28: #2 predicted fragment length is 30 bps INFO @ Mon, 12 Aug 2019 18:03:28: #2 alternative fragment length(s) may be 4,30,494,574,592 bps INFO @ Mon, 12 Aug 2019 18:03:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.05_model.r WARNING @ Mon, 12 Aug 2019 18:03:28: #2 Since the d (30) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:03:28: #2 You may need to consider one of the other alternative d(s): 4,30,494,574,592 WARNING @ Mon, 12 Aug 2019 18:03:28: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:03:28: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:03:28: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:03:29: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:03:29: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:03:29: #1 total tags in treatment: 3975159 INFO @ Mon, 12 Aug 2019 18:03:29: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:03:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:03:29: #1 tags after filtering in treatment: 3975159 INFO @ Mon, 12 Aug 2019 18:03:29: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:03:29: #1 finished! INFO @ Mon, 12 Aug 2019 18:03:29: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:03:29: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:03:30: #2 number of paired peaks: 403 WARNING @ Mon, 12 Aug 2019 18:03:30: Fewer paired peaks (403) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 403 pairs to build model! INFO @ Mon, 12 Aug 2019 18:03:30: start model_add_line... INFO @ Mon, 12 Aug 2019 18:03:30: start X-correlation... INFO @ Mon, 12 Aug 2019 18:03:30: end of X-cor INFO @ Mon, 12 Aug 2019 18:03:30: #2 finished! INFO @ Mon, 12 Aug 2019 18:03:30: #2 predicted fragment length is 30 bps INFO @ Mon, 12 Aug 2019 18:03:30: #2 alternative fragment length(s) may be 4,30,494,574,592 bps INFO @ Mon, 12 Aug 2019 18:03:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.10_model.r WARNING @ Mon, 12 Aug 2019 18:03:30: #2 Since the d (30) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:03:30: #2 You may need to consider one of the other alternative d(s): 4,30,494,574,592 WARNING @ Mon, 12 Aug 2019 18:03:30: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:03:30: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:03:30: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:03:33: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 18:03:33: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 18:03:33: #1 total tags in treatment: 3975159 INFO @ Mon, 12 Aug 2019 18:03:33: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:03:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:03:33: #1 tags after filtering in treatment: 3975159 INFO @ Mon, 12 Aug 2019 18:03:33: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:03:33: #1 finished! INFO @ Mon, 12 Aug 2019 18:03:33: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:03:33: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:03:34: #2 number of paired peaks: 403 WARNING @ Mon, 12 Aug 2019 18:03:34: Fewer paired peaks (403) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 403 pairs to build model! INFO @ Mon, 12 Aug 2019 18:03:34: start model_add_line... INFO @ Mon, 12 Aug 2019 18:03:34: start X-correlation... INFO @ Mon, 12 Aug 2019 18:03:34: end of X-cor INFO @ Mon, 12 Aug 2019 18:03:34: #2 finished! INFO @ Mon, 12 Aug 2019 18:03:34: #2 predicted fragment length is 30 bps INFO @ Mon, 12 Aug 2019 18:03:34: #2 alternative fragment length(s) may be 4,30,494,574,592 bps INFO @ Mon, 12 Aug 2019 18:03:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.20_model.r WARNING @ Mon, 12 Aug 2019 18:03:34: #2 Since the d (30) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 18:03:34: #2 You may need to consider one of the other alternative d(s): 4,30,494,574,592 WARNING @ Mon, 12 Aug 2019 18:03:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 18:03:34: #3 Call peaks... INFO @ Mon, 12 Aug 2019 18:03:34: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 18:03:40: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:03:41: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:03:45: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.05_peaks.xls INFO @ Mon, 12 Aug 2019 18:03:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:03:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.05_summits.bed INFO @ Mon, 12 Aug 2019 18:03:45: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (374 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:03:46: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 18:03:47: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.10_peaks.xls INFO @ Mon, 12 Aug 2019 18:03:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:03:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.10_summits.bed INFO @ Mon, 12 Aug 2019 18:03:47: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (189 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:03:51: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.20_peaks.xls INFO @ Mon, 12 Aug 2019 18:03:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 18:03:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX276917/SRX276917.20_summits.bed INFO @ Mon, 12 Aug 2019 18:03:51: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (58 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。