Job ID = 9157265 sra ファイルのダウンロード中... Completed: 595243K bytes transferred in 6 seconds (714552K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 13170882 spots for /home/okishinya/chipatlas/results/ce10/SRX2350746/SRR5024055.sra Written 13170882 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:13 13170882 reads; of these: 13170882 (100.00%) were unpaired; of these: 746970 (5.67%) aligned 0 times 11016336 (83.64%) aligned exactly 1 time 1407576 (10.69%) aligned >1 times 94.33% overall alignment rate Time searching: 00:03:13 Overall time: 00:03:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1627042 / 12423912 = 0.1310 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 11:20:35: # Command line: callpeak -t SRX2350746.bam -f BAM -g ce -n SRX2350746.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2350746.10 # format = BAM # ChIP-seq file = ['SRX2350746.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:20:35: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:20:35: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:20:35: # Command line: callpeak -t SRX2350746.bam -f BAM -g ce -n SRX2350746.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2350746.20 # format = BAM # ChIP-seq file = ['SRX2350746.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:20:35: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:20:35: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:20:35: # Command line: callpeak -t SRX2350746.bam -f BAM -g ce -n SRX2350746.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2350746.05 # format = BAM # ChIP-seq file = ['SRX2350746.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:20:35: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:20:35: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:20:44: 1000000 INFO @ Tue, 27 Jun 2017 11:20:44: 1000000 INFO @ Tue, 27 Jun 2017 11:20:44: 1000000 INFO @ Tue, 27 Jun 2017 11:20:53: 2000000 INFO @ Tue, 27 Jun 2017 11:20:53: 2000000 INFO @ Tue, 27 Jun 2017 11:20:53: 2000000 INFO @ Tue, 27 Jun 2017 11:21:02: 3000000 INFO @ Tue, 27 Jun 2017 11:21:02: 3000000 INFO @ Tue, 27 Jun 2017 11:21:02: 3000000 INFO @ Tue, 27 Jun 2017 11:21:11: 4000000 INFO @ Tue, 27 Jun 2017 11:21:11: 4000000 INFO @ Tue, 27 Jun 2017 11:21:11: 4000000 INFO @ Tue, 27 Jun 2017 11:21:19: 5000000 INFO @ Tue, 27 Jun 2017 11:21:19: 5000000 INFO @ Tue, 27 Jun 2017 11:21:19: 5000000 INFO @ Tue, 27 Jun 2017 11:21:27: 6000000 INFO @ Tue, 27 Jun 2017 11:21:27: 6000000 INFO @ Tue, 27 Jun 2017 11:21:27: 6000000 INFO @ Tue, 27 Jun 2017 11:21:35: 7000000 INFO @ Tue, 27 Jun 2017 11:21:35: 7000000 INFO @ Tue, 27 Jun 2017 11:21:35: 7000000 INFO @ Tue, 27 Jun 2017 11:21:42: 8000000 INFO @ Tue, 27 Jun 2017 11:21:42: 8000000 INFO @ Tue, 27 Jun 2017 11:21:42: 8000000 INFO @ Tue, 27 Jun 2017 11:21:50: 9000000 INFO @ Tue, 27 Jun 2017 11:21:50: 9000000 INFO @ Tue, 27 Jun 2017 11:21:50: 9000000 INFO @ Tue, 27 Jun 2017 11:21:58: 10000000 INFO @ Tue, 27 Jun 2017 11:21:58: 10000000 INFO @ Tue, 27 Jun 2017 11:21:58: 10000000 INFO @ Tue, 27 Jun 2017 11:22:04: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 11:22:04: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 11:22:04: #1 total tags in treatment: 10796870 INFO @ Tue, 27 Jun 2017 11:22:04: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:22:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:22:04: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 11:22:04: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 11:22:04: #1 total tags in treatment: 10796870 INFO @ Tue, 27 Jun 2017 11:22:04: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:22:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:22:04: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 11:22:04: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 11:22:04: #1 total tags in treatment: 10796870 INFO @ Tue, 27 Jun 2017 11:22:04: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:22:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:22:04: #1 tags after filtering in treatment: 10796870 INFO @ Tue, 27 Jun 2017 11:22:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:22:04: #1 finished! INFO @ Tue, 27 Jun 2017 11:22:04: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:22:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:22:04: #1 tags after filtering in treatment: 10796870 INFO @ Tue, 27 Jun 2017 11:22:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:22:04: #1 finished! INFO @ Tue, 27 Jun 2017 11:22:04: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:22:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:22:04: #1 tags after filtering in treatment: 10796870 INFO @ Tue, 27 Jun 2017 11:22:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:22:04: #1 finished! INFO @ Tue, 27 Jun 2017 11:22:04: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:22:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:22:05: #2 number of paired peaks: 68 WARNING @ Tue, 27 Jun 2017 11:22:05: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:22:05: Process for pairing-model is terminated! INFO @ Tue, 27 Jun 2017 11:22:05: #2 number of paired peaks: 68 WARNING @ Tue, 27 Jun 2017 11:22:05: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:22:05: Process for pairing-model is terminated! INFO @ Tue, 27 Jun 2017 11:22:05: #2 number of paired peaks: 68 WARNING @ Tue, 27 Jun 2017 11:22:05: Too few paired peaks (68) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:22:05: Process for pairing-model is terminated! cat: SRX2350746.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX2350746.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) cat: SRX2350746.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis rm: cannot remove `SRX2350746.10_model.r': そのようなファイルやディレクトリはありません needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2350746.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2350746.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling rm: cannot remove `SRX2350746.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2350746.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2350746.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2350746.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2350746.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2350746.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。