Job ID = 10609067 sra ファイルのダウンロード中... Completed: 1634228K bytes transferred in 19 seconds (694309K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 53777861 spots for /home/okishinya/chipatlas/results/ce10/SRX2249335/SRR4427774.sra Written 53777861 spots for /home/okishinya/chipatlas/results/ce10/SRX2249335/SRR4427774.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:24:47 53777861 reads; of these: 53777861 (100.00%) were unpaired; of these: 5669017 (10.54%) aligned 0 times 38469673 (71.53%) aligned exactly 1 time 9639171 (17.92%) aligned >1 times 89.46% overall alignment rate Time searching: 00:24:48 Overall time: 00:24:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 13773946 / 48108844 = 0.2863 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 04 May 2018 07:46:06: # Command line: callpeak -t SRX2249335.bam -f BAM -g ce -n SRX2249335.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2249335.05 # format = BAM # ChIP-seq file = ['SRX2249335.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 04 May 2018 07:46:06: # Command line: callpeak -t SRX2249335.bam -f BAM -g ce -n SRX2249335.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2249335.20 # format = BAM # ChIP-seq file = ['SRX2249335.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 04 May 2018 07:46:06: # Command line: callpeak -t SRX2249335.bam -f BAM -g ce -n SRX2249335.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2249335.10 # format = BAM # ChIP-seq file = ['SRX2249335.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 04 May 2018 07:46:06: #1 read tag files... INFO @ Fri, 04 May 2018 07:46:06: #1 read tag files... INFO @ Fri, 04 May 2018 07:46:06: #1 read tag files... INFO @ Fri, 04 May 2018 07:46:06: #1 read treatment tags... INFO @ Fri, 04 May 2018 07:46:06: #1 read treatment tags... INFO @ Fri, 04 May 2018 07:46:06: #1 read treatment tags... INFO @ Fri, 04 May 2018 07:46:14: 1000000 INFO @ Fri, 04 May 2018 07:46:15: 1000000 INFO @ Fri, 04 May 2018 07:46:15: 1000000 INFO @ Fri, 04 May 2018 07:46:23: 2000000 INFO @ Fri, 04 May 2018 07:46:23: 2000000 INFO @ Fri, 04 May 2018 07:46:23: 2000000 INFO @ Fri, 04 May 2018 07:46:31: 3000000 INFO @ Fri, 04 May 2018 07:46:31: 3000000 INFO @ Fri, 04 May 2018 07:46:32: 3000000 INFO @ Fri, 04 May 2018 07:46:39: 4000000 INFO @ Fri, 04 May 2018 07:46:40: 4000000 INFO @ Fri, 04 May 2018 07:46:40: 4000000 INFO @ Fri, 04 May 2018 07:46:48: 5000000 INFO @ Fri, 04 May 2018 07:46:48: 5000000 INFO @ Fri, 04 May 2018 07:46:49: 5000000 INFO @ Fri, 04 May 2018 07:46:56: 6000000 INFO @ Fri, 04 May 2018 07:46:57: 6000000 INFO @ Fri, 04 May 2018 07:46:57: 6000000 INFO @ Fri, 04 May 2018 07:47:04: 7000000 INFO @ Fri, 04 May 2018 07:47:05: 7000000 INFO @ Fri, 04 May 2018 07:47:06: 7000000 INFO @ Fri, 04 May 2018 07:47:12: 8000000 INFO @ Fri, 04 May 2018 07:47:14: 8000000 INFO @ Fri, 04 May 2018 07:47:14: 8000000 INFO @ Fri, 04 May 2018 07:47:20: 9000000 INFO @ Fri, 04 May 2018 07:47:22: 9000000 INFO @ Fri, 04 May 2018 07:47:23: 9000000 INFO @ Fri, 04 May 2018 07:47:28: 10000000 INFO @ Fri, 04 May 2018 07:47:31: 10000000 INFO @ Fri, 04 May 2018 07:47:32: 10000000 INFO @ Fri, 04 May 2018 07:47:37: 11000000 INFO @ Fri, 04 May 2018 07:47:40: 11000000 INFO @ Fri, 04 May 2018 07:47:40: 11000000 INFO @ Fri, 04 May 2018 07:47:45: 12000000 INFO @ Fri, 04 May 2018 07:47:48: 12000000 INFO @ Fri, 04 May 2018 07:47:49: 12000000 INFO @ Fri, 04 May 2018 07:47:53: 13000000 INFO @ Fri, 04 May 2018 07:47:57: 13000000 INFO @ Fri, 04 May 2018 07:47:58: 13000000 INFO @ Fri, 04 May 2018 07:48:01: 14000000 INFO @ Fri, 04 May 2018 07:48:06: 14000000 INFO @ Fri, 04 May 2018 07:48:07: 14000000 INFO @ Fri, 04 May 2018 07:48:10: 15000000 INFO @ Fri, 04 May 2018 07:48:15: 15000000 INFO @ Fri, 04 May 2018 07:48:16: 15000000 INFO @ Fri, 04 May 2018 07:48:18: 16000000 INFO @ Fri, 04 May 2018 07:48:24: 16000000 INFO @ Fri, 04 May 2018 07:48:25: 16000000 INFO @ Fri, 04 May 2018 07:48:26: 17000000 INFO @ Fri, 04 May 2018 07:48:33: 17000000 INFO @ Fri, 04 May 2018 07:48:34: 18000000 INFO @ Fri, 04 May 2018 07:48:34: 17000000 INFO @ Fri, 04 May 2018 07:48:42: 18000000 INFO @ Fri, 04 May 2018 07:48:42: 19000000 INFO @ Fri, 04 May 2018 07:48:43: 18000000 INFO @ Fri, 04 May 2018 07:48:50: 20000000 INFO @ Fri, 04 May 2018 07:48:51: 19000000 INFO @ Fri, 04 May 2018 07:48:52: 19000000 INFO @ Fri, 04 May 2018 07:48:58: 21000000 INFO @ Fri, 04 May 2018 07:49:00: 20000000 INFO @ Fri, 04 May 2018 07:49:01: 20000000 INFO @ Fri, 04 May 2018 07:49:07: 22000000 INFO @ Fri, 04 May 2018 07:49:09: 21000000 INFO @ Fri, 04 May 2018 07:49:10: 21000000 INFO @ Fri, 04 May 2018 07:49:15: 23000000 INFO @ Fri, 04 May 2018 07:49:18: 22000000 INFO @ Fri, 04 May 2018 07:49:19: 22000000 INFO @ Fri, 04 May 2018 07:49:23: 24000000 INFO @ Fri, 04 May 2018 07:49:26: 23000000 INFO @ Fri, 04 May 2018 07:49:28: 23000000 INFO @ Fri, 04 May 2018 07:49:31: 25000000 INFO @ Fri, 04 May 2018 07:49:35: 24000000 INFO @ Fri, 04 May 2018 07:49:37: 24000000 INFO @ Fri, 04 May 2018 07:49:39: 26000000 INFO @ Fri, 04 May 2018 07:49:44: 25000000 INFO @ Fri, 04 May 2018 07:49:46: 25000000 INFO @ Fri, 04 May 2018 07:49:47: 27000000 INFO @ Fri, 04 May 2018 07:49:53: 26000000 INFO @ Fri, 04 May 2018 07:49:55: 26000000 INFO @ Fri, 04 May 2018 07:49:55: 28000000 INFO @ Fri, 04 May 2018 07:50:02: 27000000 INFO @ Fri, 04 May 2018 07:50:03: 29000000 INFO @ Fri, 04 May 2018 07:50:04: 27000000 INFO @ Fri, 04 May 2018 07:50:11: 28000000 INFO @ Fri, 04 May 2018 07:50:11: 30000000 INFO @ Fri, 04 May 2018 07:50:13: 28000000 INFO @ Fri, 04 May 2018 07:50:19: 31000000 INFO @ Fri, 04 May 2018 07:50:20: 29000000 INFO @ Fri, 04 May 2018 07:50:21: 29000000 INFO @ Fri, 04 May 2018 07:50:27: 32000000 INFO @ Fri, 04 May 2018 07:50:29: 30000000 INFO @ Fri, 04 May 2018 07:50:30: 30000000 INFO @ Fri, 04 May 2018 07:50:35: 33000000 INFO @ Fri, 04 May 2018 07:50:37: 31000000 INFO @ Fri, 04 May 2018 07:50:40: 31000000 INFO @ Fri, 04 May 2018 07:50:44: 34000000 INFO @ Fri, 04 May 2018 07:50:47: 32000000 INFO @ Fri, 04 May 2018 07:50:47: #1 tag size is determined as 101 bps INFO @ Fri, 04 May 2018 07:50:47: #1 tag size = 101 INFO @ Fri, 04 May 2018 07:50:47: #1 total tags in treatment: 34334898 INFO @ Fri, 04 May 2018 07:50:47: #1 user defined the maximum tags... INFO @ Fri, 04 May 2018 07:50:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 04 May 2018 07:50:47: #1 tags after filtering in treatment: 34334898 INFO @ Fri, 04 May 2018 07:50:47: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 04 May 2018 07:50:47: #1 finished! INFO @ Fri, 04 May 2018 07:50:47: #2 Build Peak Model... INFO @ Fri, 04 May 2018 07:50:47: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 04 May 2018 07:50:49: 32000000 INFO @ Fri, 04 May 2018 07:50:49: #2 number of paired peaks: 37 WARNING @ Fri, 04 May 2018 07:50:49: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 04 May 2018 07:50:49: Process for pairing-model is terminated! cat: SRX2249335.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2249335.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2249335.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2249335.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 04 May 2018 07:50:55: 33000000 INFO @ Fri, 04 May 2018 07:50:58: 33000000 INFO @ Fri, 04 May 2018 07:51:04: 34000000 INFO @ Fri, 04 May 2018 07:51:06: 34000000 INFO @ Fri, 04 May 2018 07:51:07: #1 tag size is determined as 101 bps INFO @ Fri, 04 May 2018 07:51:07: #1 tag size = 101 INFO @ Fri, 04 May 2018 07:51:07: #1 total tags in treatment: 34334898 INFO @ Fri, 04 May 2018 07:51:07: #1 user defined the maximum tags... INFO @ Fri, 04 May 2018 07:51:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 04 May 2018 07:51:08: #1 tags after filtering in treatment: 34334898 INFO @ Fri, 04 May 2018 07:51:08: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 04 May 2018 07:51:08: #1 finished! INFO @ Fri, 04 May 2018 07:51:08: #2 Build Peak Model... INFO @ Fri, 04 May 2018 07:51:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 04 May 2018 07:51:09: #1 tag size is determined as 101 bps INFO @ Fri, 04 May 2018 07:51:09: #1 tag size = 101 INFO @ Fri, 04 May 2018 07:51:09: #1 total tags in treatment: 34334898 INFO @ Fri, 04 May 2018 07:51:09: #1 user defined the maximum tags... INFO @ Fri, 04 May 2018 07:51:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 04 May 2018 07:51:10: #2 number of paired peaks: 37 WARNING @ Fri, 04 May 2018 07:51:10: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 04 May 2018 07:51:10: Process for pairing-model is terminated! cat: SRX2249335.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2249335.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2249335.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2249335.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 04 May 2018 07:51:10: #1 tags after filtering in treatment: 34334898 INFO @ Fri, 04 May 2018 07:51:10: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 04 May 2018 07:51:10: #1 finished! INFO @ Fri, 04 May 2018 07:51:10: #2 Build Peak Model... INFO @ Fri, 04 May 2018 07:51:10: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 04 May 2018 07:51:12: #2 number of paired peaks: 37 WARNING @ Fri, 04 May 2018 07:51:12: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 04 May 2018 07:51:12: Process for pairing-model is terminated! cat: SRX2249335.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2249335.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2249335.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2249335.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。