Job ID = 9157126 sra ファイルのダウンロード中... Completed: 616871K bytes transferred in 7 seconds (648345K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 18668481 spots for /home/okishinya/chipatlas/results/ce10/SRX2228865/SRR4380321.sra Written 18668481 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:59 18668481 reads; of these: 18668481 (100.00%) were unpaired; of these: 696995 (3.73%) aligned 0 times 14977944 (80.23%) aligned exactly 1 time 2993542 (16.04%) aligned >1 times 96.27% overall alignment rate Time searching: 00:04:59 Overall time: 00:04:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 12306428 / 17971486 = 0.6848 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 10:45:16: # Command line: callpeak -t SRX2228865.bam -f BAM -g ce -n SRX2228865.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2228865.10 # format = BAM # ChIP-seq file = ['SRX2228865.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 10:45:16: # Command line: callpeak -t SRX2228865.bam -f BAM -g ce -n SRX2228865.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2228865.05 # format = BAM # ChIP-seq file = ['SRX2228865.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 10:45:16: #1 read tag files... INFO @ Tue, 27 Jun 2017 10:45:16: #1 read tag files... INFO @ Tue, 27 Jun 2017 10:45:16: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 10:45:16: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 10:45:16: # Command line: callpeak -t SRX2228865.bam -f BAM -g ce -n SRX2228865.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2228865.20 # format = BAM # ChIP-seq file = ['SRX2228865.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 10:45:16: #1 read tag files... INFO @ Tue, 27 Jun 2017 10:45:16: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 10:45:25: 1000000 INFO @ Tue, 27 Jun 2017 10:45:25: 1000000 INFO @ Tue, 27 Jun 2017 10:45:25: 1000000 INFO @ Tue, 27 Jun 2017 10:45:34: 2000000 INFO @ Tue, 27 Jun 2017 10:45:34: 2000000 INFO @ Tue, 27 Jun 2017 10:45:34: 2000000 INFO @ Tue, 27 Jun 2017 10:45:43: 3000000 INFO @ Tue, 27 Jun 2017 10:45:43: 3000000 INFO @ Tue, 27 Jun 2017 10:45:43: 3000000 INFO @ Tue, 27 Jun 2017 10:45:52: 4000000 INFO @ Tue, 27 Jun 2017 10:45:52: 4000000 INFO @ Tue, 27 Jun 2017 10:45:52: 4000000 INFO @ Tue, 27 Jun 2017 10:46:01: 5000000 INFO @ Tue, 27 Jun 2017 10:46:01: 5000000 INFO @ Tue, 27 Jun 2017 10:46:01: 5000000 INFO @ Tue, 27 Jun 2017 10:46:06: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 10:46:06: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 10:46:06: #1 total tags in treatment: 5665058 INFO @ Tue, 27 Jun 2017 10:46:06: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 10:46:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 10:46:06: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 10:46:06: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 10:46:06: #1 total tags in treatment: 5665058 INFO @ Tue, 27 Jun 2017 10:46:06: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 10:46:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 10:46:06: #1 tags after filtering in treatment: 5665058 INFO @ Tue, 27 Jun 2017 10:46:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 10:46:06: #1 finished! INFO @ Tue, 27 Jun 2017 10:46:06: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 10:46:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 10:46:06: #1 tags after filtering in treatment: 5665058 INFO @ Tue, 27 Jun 2017 10:46:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 10:46:06: #1 finished! INFO @ Tue, 27 Jun 2017 10:46:06: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 10:46:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 10:46:07: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 10:46:07: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 10:46:07: #1 total tags in treatment: 5665058 INFO @ Tue, 27 Jun 2017 10:46:07: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 10:46:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 10:46:07: #1 tags after filtering in treatment: 5665058 INFO @ Tue, 27 Jun 2017 10:46:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 10:46:07: #1 finished! INFO @ Tue, 27 Jun 2017 10:46:07: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 10:46:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 10:46:07: #2 number of paired peaks: 1036 INFO @ Tue, 27 Jun 2017 10:46:07: start model_add_line... INFO @ Tue, 27 Jun 2017 10:46:07: #2 number of paired peaks: 1036 INFO @ Tue, 27 Jun 2017 10:46:07: start model_add_line... INFO @ Tue, 27 Jun 2017 10:46:07: start X-correlation... INFO @ Tue, 27 Jun 2017 10:46:07: end of X-cor INFO @ Tue, 27 Jun 2017 10:46:07: #2 finished! INFO @ Tue, 27 Jun 2017 10:46:07: #2 predicted fragment length is 134 bps INFO @ Tue, 27 Jun 2017 10:46:07: #2 alternative fragment length(s) may be 134 bps INFO @ Tue, 27 Jun 2017 10:46:07: #2.2 Generate R script for model : SRX2228865.10_model.r INFO @ Tue, 27 Jun 2017 10:46:07: #3 Call peaks... INFO @ Tue, 27 Jun 2017 10:46:07: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 27 Jun 2017 10:46:07: start X-correlation... INFO @ Tue, 27 Jun 2017 10:46:07: end of X-cor INFO @ Tue, 27 Jun 2017 10:46:07: #2 finished! INFO @ Tue, 27 Jun 2017 10:46:07: #2 predicted fragment length is 134 bps INFO @ Tue, 27 Jun 2017 10:46:07: #2 alternative fragment length(s) may be 134 bps INFO @ Tue, 27 Jun 2017 10:46:07: #2.2 Generate R script for model : SRX2228865.20_model.r INFO @ Tue, 27 Jun 2017 10:46:07: #3 Call peaks... INFO @ Tue, 27 Jun 2017 10:46:07: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 27 Jun 2017 10:46:07: #2 number of paired peaks: 1036 INFO @ Tue, 27 Jun 2017 10:46:07: start model_add_line... INFO @ Tue, 27 Jun 2017 10:46:07: start X-correlation... INFO @ Tue, 27 Jun 2017 10:46:07: end of X-cor INFO @ Tue, 27 Jun 2017 10:46:07: #2 finished! INFO @ Tue, 27 Jun 2017 10:46:07: #2 predicted fragment length is 134 bps INFO @ Tue, 27 Jun 2017 10:46:07: #2 alternative fragment length(s) may be 134 bps INFO @ Tue, 27 Jun 2017 10:46:07: #2.2 Generate R script for model : SRX2228865.05_model.r INFO @ Tue, 27 Jun 2017 10:46:07: #3 Call peaks... INFO @ Tue, 27 Jun 2017 10:46:07: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 27 Jun 2017 10:46:22: #3 Call peaks for each chromosome... INFO @ Tue, 27 Jun 2017 10:46:22: #3 Call peaks for each chromosome... INFO @ Tue, 27 Jun 2017 10:46:23: #3 Call peaks for each chromosome... INFO @ Tue, 27 Jun 2017 10:46:30: #4 Write output xls file... SRX2228865.20_peaks.xls INFO @ Tue, 27 Jun 2017 10:46:30: #4 Write peak in narrowPeak format file... SRX2228865.20_peaks.narrowPeak INFO @ Tue, 27 Jun 2017 10:46:30: #4 Write summits bed file... SRX2228865.20_summits.bed INFO @ Tue, 27 Jun 2017 10:46:30: Done! INFO @ Tue, 27 Jun 2017 10:46:30: #4 Write output xls file... SRX2228865.05_peaks.xls INFO @ Tue, 27 Jun 2017 10:46:31: #4 Write peak in narrowPeak format file... SRX2228865.05_peaks.narrowPeak INFO @ Tue, 27 Jun 2017 10:46:31: #4 Write summits bed file... SRX2228865.05_summits.bed pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (645 records, 4 fields): 2 millis INFO @ Tue, 27 Jun 2017 10:46:31: Done! CompletedMACS2peakCalling pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1614 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 10:46:31: #4 Write output xls file... SRX2228865.10_peaks.xls INFO @ Tue, 27 Jun 2017 10:46:31: #4 Write peak in narrowPeak format file... SRX2228865.10_peaks.narrowPeak INFO @ Tue, 27 Jun 2017 10:46:31: #4 Write summits bed file... SRX2228865.10_summits.bed INFO @ Tue, 27 Jun 2017 10:46:31: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1056 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。