Job ID = 9025641 sra ファイルのダウンロード中... Completed: 125995K bytes transferred in 4 seconds (210880K bits/sec), in 1 file, 2 directories. % Total % Received % Xferd Average Speed Time Time Time Current Dload Upload Total Spent Left Speed 0 0 0 0 0 0 0 0 --:--:-- 0:00:06 --:--:-- 0 100 7663 0 7663 0 0 1057 0 --:--:-- 0:00:07 --:--:-- 7932 100 30318 0 30318 0 0 3753 0 --:--:-- 0:00:08 --:--:-- 16890 100 47093 0 47093 0 0 5712 0 --:--:-- 0:00:08 --:--:-- 24002 sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 5401926 spots for /home/okishinya/chipatlas/results/ce10/SRX2144167/SRR4188771.sra Written 5401926 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:35 5401926 reads; of these: 5401926 (100.00%) were unpaired; of these: 938759 (17.38%) aligned 0 times 3560803 (65.92%) aligned exactly 1 time 902364 (16.70%) aligned >1 times 82.62% overall alignment rate Time searching: 00:01:36 Overall time: 00:01:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 261881 / 4463167 = 0.0587 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 03 Jun 2017 04:48:03: # Command line: callpeak -t SRX2144167.bam -f BAM -g ce -n SRX2144167.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2144167.10 # format = BAM # ChIP-seq file = ['SRX2144167.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Sat, 03 Jun 2017 04:48:03: #1 read tag files... INFO @ Sat, 03 Jun 2017 04:48:03: #1 read treatment tags... INFO @ Sat, 03 Jun 2017 04:48:03: # Command line: callpeak -t SRX2144167.bam -f BAM -g ce -n SRX2144167.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2144167.20 # format = BAM # ChIP-seq file = ['SRX2144167.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Sat, 03 Jun 2017 04:48:03: #1 read tag files... INFO @ Sat, 03 Jun 2017 04:48:03: #1 read treatment tags... INFO @ Sat, 03 Jun 2017 04:48:03: # Command line: callpeak -t SRX2144167.bam -f BAM -g ce -n SRX2144167.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2144167.05 # format = BAM # ChIP-seq file = ['SRX2144167.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Sat, 03 Jun 2017 04:48:03: #1 read tag files... INFO @ Sat, 03 Jun 2017 04:48:03: #1 read treatment tags... INFO @ Sat, 03 Jun 2017 04:48:09: 1000000 INFO @ Sat, 03 Jun 2017 04:48:11: 1000000 INFO @ Sat, 03 Jun 2017 04:48:11: 1000000 INFO @ Sat, 03 Jun 2017 04:48:16: 2000000 INFO @ Sat, 03 Jun 2017 04:48:18: 2000000 INFO @ Sat, 03 Jun 2017 04:48:19: 2000000 INFO @ Sat, 03 Jun 2017 04:48:22: 3000000 INFO @ Sat, 03 Jun 2017 04:48:25: 3000000 INFO @ Sat, 03 Jun 2017 04:48:26: 3000000 INFO @ Sat, 03 Jun 2017 04:48:29: 4000000 INFO @ Sat, 03 Jun 2017 04:48:30: #1 tag size is determined as 50 bps INFO @ Sat, 03 Jun 2017 04:48:30: #1 tag size = 50 INFO @ Sat, 03 Jun 2017 04:48:30: #1 total tags in treatment: 4201286 INFO @ Sat, 03 Jun 2017 04:48:30: #1 user defined the maximum tags... INFO @ Sat, 03 Jun 2017 04:48:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Jun 2017 04:48:31: #1 tags after filtering in treatment: 4200643 INFO @ Sat, 03 Jun 2017 04:48:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Jun 2017 04:48:31: #1 finished! INFO @ Sat, 03 Jun 2017 04:48:31: #2 Build Peak Model... INFO @ Sat, 03 Jun 2017 04:48:32: 4000000 INFO @ Sat, 03 Jun 2017 04:48:32: #2 number of paired peaks: 430 WARNING @ Sat, 03 Jun 2017 04:48:32: Fewer paired peaks (430) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 430 pairs to build model! INFO @ Sat, 03 Jun 2017 04:48:32: start model_add_line... INFO @ Sat, 03 Jun 2017 04:48:33: #1 tag size is determined as 50 bps INFO @ Sat, 03 Jun 2017 04:48:33: #1 tag size = 50 INFO @ Sat, 03 Jun 2017 04:48:33: #1 total tags in treatment: 4201286 INFO @ Sat, 03 Jun 2017 04:48:33: #1 user defined the maximum tags... INFO @ Sat, 03 Jun 2017 04:48:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Jun 2017 04:48:34: 4000000 INFO @ Sat, 03 Jun 2017 04:48:34: #1 tags after filtering in treatment: 4200643 INFO @ Sat, 03 Jun 2017 04:48:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Jun 2017 04:48:34: #1 finished! INFO @ Sat, 03 Jun 2017 04:48:34: #2 Build Peak Model... INFO @ Sat, 03 Jun 2017 04:48:34: #2 number of paired peaks: 430 WARNING @ Sat, 03 Jun 2017 04:48:34: Fewer paired peaks (430) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 430 pairs to build model! INFO @ Sat, 03 Jun 2017 04:48:34: start model_add_line... INFO @ Sat, 03 Jun 2017 04:48:35: #1 tag size is determined as 50 bps INFO @ Sat, 03 Jun 2017 04:48:35: #1 tag size = 50 INFO @ Sat, 03 Jun 2017 04:48:35: #1 total tags in treatment: 4201286 INFO @ Sat, 03 Jun 2017 04:48:35: #1 user defined the maximum tags... INFO @ Sat, 03 Jun 2017 04:48:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Jun 2017 04:48:35: start X-correlation... INFO @ Sat, 03 Jun 2017 04:48:35: end of X-cor INFO @ Sat, 03 Jun 2017 04:48:35: #2 finished! INFO @ Sat, 03 Jun 2017 04:48:35: #2 predicted fragment length is 48 bps INFO @ Sat, 03 Jun 2017 04:48:35: #2 alternative fragment length(s) may be 4,48 bps INFO @ Sat, 03 Jun 2017 04:48:35: #2.2 Generate R script for model : SRX2144167.10_model.r WARNING @ Sat, 03 Jun 2017 04:48:35: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Jun 2017 04:48:35: #2 You may need to consider one of the other alternative d(s): 4,48 WARNING @ Sat, 03 Jun 2017 04:48:35: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Jun 2017 04:48:35: #3 Call peaks... INFO @ Sat, 03 Jun 2017 04:48:35: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Jun 2017 04:48:36: #1 tags after filtering in treatment: 4200643 INFO @ Sat, 03 Jun 2017 04:48:36: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Jun 2017 04:48:36: #1 finished! INFO @ Sat, 03 Jun 2017 04:48:36: #2 Build Peak Model... INFO @ Sat, 03 Jun 2017 04:48:36: #2 number of paired peaks: 430 WARNING @ Sat, 03 Jun 2017 04:48:36: Fewer paired peaks (430) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 430 pairs to build model! INFO @ Sat, 03 Jun 2017 04:48:36: start model_add_line... INFO @ Sat, 03 Jun 2017 04:48:38: start X-correlation... INFO @ Sat, 03 Jun 2017 04:48:38: end of X-cor INFO @ Sat, 03 Jun 2017 04:48:38: #2 finished! INFO @ Sat, 03 Jun 2017 04:48:38: #2 predicted fragment length is 48 bps INFO @ Sat, 03 Jun 2017 04:48:38: #2 alternative fragment length(s) may be 4,48 bps INFO @ Sat, 03 Jun 2017 04:48:38: #2.2 Generate R script for model : SRX2144167.20_model.r WARNING @ Sat, 03 Jun 2017 04:48:38: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Jun 2017 04:48:38: #2 You may need to consider one of the other alternative d(s): 4,48 WARNING @ Sat, 03 Jun 2017 04:48:38: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Jun 2017 04:48:38: #3 Call peaks... INFO @ Sat, 03 Jun 2017 04:48:38: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Jun 2017 04:48:40: start X-correlation... INFO @ Sat, 03 Jun 2017 04:48:40: end of X-cor INFO @ Sat, 03 Jun 2017 04:48:40: #2 finished! INFO @ Sat, 03 Jun 2017 04:48:40: #2 predicted fragment length is 48 bps INFO @ Sat, 03 Jun 2017 04:48:40: #2 alternative fragment length(s) may be 4,48 bps INFO @ Sat, 03 Jun 2017 04:48:40: #2.2 Generate R script for model : SRX2144167.05_model.r WARNING @ Sat, 03 Jun 2017 04:48:40: #2 Since the d (48) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Jun 2017 04:48:40: #2 You may need to consider one of the other alternative d(s): 4,48 WARNING @ Sat, 03 Jun 2017 04:48:40: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Jun 2017 04:48:40: #3 Call peaks... INFO @ Sat, 03 Jun 2017 04:48:40: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Jun 2017 04:49:01: #3 Call peaks for each chromosome... INFO @ Sat, 03 Jun 2017 04:49:02: #3 Call peaks for each chromosome... INFO @ Sat, 03 Jun 2017 04:49:04: #3 Call peaks for each chromosome... INFO @ Sat, 03 Jun 2017 04:49:19: #4 Write output xls file... SRX2144167.10_peaks.xls INFO @ Sat, 03 Jun 2017 04:49:19: #4 Write peak in narrowPeak format file... SRX2144167.10_peaks.narrowPeak INFO @ Sat, 03 Jun 2017 04:49:19: #4 Write summits bed file... SRX2144167.10_summits.bed INFO @ Sat, 03 Jun 2017 04:49:19: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (334 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sat, 03 Jun 2017 04:49:20: #4 Write output xls file... SRX2144167.20_peaks.xls INFO @ Sat, 03 Jun 2017 04:49:20: #4 Write peak in narrowPeak format file... SRX2144167.20_peaks.narrowPeak INFO @ Sat, 03 Jun 2017 04:49:20: #4 Write summits bed file... SRX2144167.20_summits.bed INFO @ Sat, 03 Jun 2017 04:49:20: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (136 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sat, 03 Jun 2017 04:49:22: #4 Write output xls file... SRX2144167.05_peaks.xls INFO @ Sat, 03 Jun 2017 04:49:22: #4 Write peak in narrowPeak format file... SRX2144167.05_peaks.narrowPeak INFO @ Sat, 03 Jun 2017 04:49:22: #4 Write summits bed file... SRX2144167.05_summits.bed INFO @ Sat, 03 Jun 2017 04:49:22: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (517 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。