Job ID = 6497344 SRX = SRX1674103 Genome = ce10 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-25T21:29:35 prefetch.2.10.7: 1) Downloading 'SRR3320149'... 2020-06-25T21:29:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-25T21:47:11 prefetch.2.10.7: HTTPS download succeed 2020-06-25T21:47:11 prefetch.2.10.7: 1) 'SRR3320149' was downloaded successfully 2020-06-25T21:47:11 prefetch.2.10.7: 'SRR3320149' has 0 unresolved dependencies Read 39983162 spots for SRR3320149/SRR3320149.sra Written 39983162 spots for SRR3320149/SRR3320149.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:07:55 39983162 reads; of these: 39983162 (100.00%) were paired; of these: 848041 (2.12%) aligned concordantly 0 times 33613182 (84.07%) aligned concordantly exactly 1 time 5521939 (13.81%) aligned concordantly >1 times ---- 848041 pairs aligned concordantly 0 times; of these: 64167 (7.57%) aligned discordantly 1 time ---- 783874 pairs aligned 0 times concordantly or discordantly; of these: 1567748 mates make up the pairs; of these: 967536 (61.72%) aligned 0 times 439082 (28.01%) aligned exactly 1 time 161130 (10.28%) aligned >1 times 98.79% overall alignment rate Time searching: 01:07:55 Overall time: 01:07:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 6240088 / 39153567 = 0.1594 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:05:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:05:34: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:05:34: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:05:42: 1000000 INFO @ Fri, 26 Jun 2020 09:05:50: 2000000 INFO @ Fri, 26 Jun 2020 09:05:58: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:06:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:06:03: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:06:03: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:06:07: 4000000 INFO @ Fri, 26 Jun 2020 09:06:11: 1000000 INFO @ Fri, 26 Jun 2020 09:06:15: 5000000 INFO @ Fri, 26 Jun 2020 09:06:20: 2000000 INFO @ Fri, 26 Jun 2020 09:06:23: 6000000 INFO @ Fri, 26 Jun 2020 09:06:29: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 09:06:32: 7000000 INFO @ Fri, 26 Jun 2020 09:06:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 09:06:33: #1 read tag files... INFO @ Fri, 26 Jun 2020 09:06:33: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 09:06:37: 4000000 INFO @ Fri, 26 Jun 2020 09:06:40: 8000000 INFO @ Fri, 26 Jun 2020 09:06:41: 1000000 INFO @ Fri, 26 Jun 2020 09:06:46: 5000000 INFO @ Fri, 26 Jun 2020 09:06:49: 9000000 INFO @ Fri, 26 Jun 2020 09:06:49: 2000000 INFO @ Fri, 26 Jun 2020 09:06:54: 6000000 INFO @ Fri, 26 Jun 2020 09:06:58: 3000000 INFO @ Fri, 26 Jun 2020 09:06:58: 10000000 INFO @ Fri, 26 Jun 2020 09:07:03: 7000000 INFO @ Fri, 26 Jun 2020 09:07:06: 4000000 INFO @ Fri, 26 Jun 2020 09:07:07: 11000000 INFO @ Fri, 26 Jun 2020 09:07:12: 8000000 INFO @ Fri, 26 Jun 2020 09:07:14: 5000000 INFO @ Fri, 26 Jun 2020 09:07:15: 12000000 INFO @ Fri, 26 Jun 2020 09:07:20: 9000000 INFO @ Fri, 26 Jun 2020 09:07:22: 6000000 INFO @ Fri, 26 Jun 2020 09:07:23: 13000000 INFO @ Fri, 26 Jun 2020 09:07:29: 10000000 INFO @ Fri, 26 Jun 2020 09:07:30: 7000000 INFO @ Fri, 26 Jun 2020 09:07:32: 14000000 INFO @ Fri, 26 Jun 2020 09:07:37: 11000000 INFO @ Fri, 26 Jun 2020 09:07:39: 8000000 INFO @ Fri, 26 Jun 2020 09:07:40: 15000000 INFO @ Fri, 26 Jun 2020 09:07:46: 12000000 INFO @ Fri, 26 Jun 2020 09:07:47: 9000000 INFO @ Fri, 26 Jun 2020 09:07:49: 16000000 INFO @ Fri, 26 Jun 2020 09:07:54: 13000000 INFO @ Fri, 26 Jun 2020 09:07:55: 10000000 INFO @ Fri, 26 Jun 2020 09:07:57: 17000000 INFO @ Fri, 26 Jun 2020 09:08:03: 14000000 INFO @ Fri, 26 Jun 2020 09:08:03: 11000000 INFO @ Fri, 26 Jun 2020 09:08:05: 18000000 INFO @ Fri, 26 Jun 2020 09:08:11: 15000000 INFO @ Fri, 26 Jun 2020 09:08:11: 12000000 INFO @ Fri, 26 Jun 2020 09:08:13: 19000000 INFO @ Fri, 26 Jun 2020 09:08:19: 13000000 INFO @ Fri, 26 Jun 2020 09:08:20: 16000000 INFO @ Fri, 26 Jun 2020 09:08:21: 20000000 INFO @ Fri, 26 Jun 2020 09:08:27: 14000000 INFO @ Fri, 26 Jun 2020 09:08:28: 17000000 INFO @ Fri, 26 Jun 2020 09:08:30: 21000000 INFO @ Fri, 26 Jun 2020 09:08:35: 15000000 INFO @ Fri, 26 Jun 2020 09:08:37: 18000000 INFO @ Fri, 26 Jun 2020 09:08:38: 22000000 INFO @ Fri, 26 Jun 2020 09:08:43: 16000000 INFO @ Fri, 26 Jun 2020 09:08:45: 19000000 INFO @ Fri, 26 Jun 2020 09:08:46: 23000000 INFO @ Fri, 26 Jun 2020 09:08:51: 17000000 INFO @ Fri, 26 Jun 2020 09:08:54: 20000000 INFO @ Fri, 26 Jun 2020 09:08:54: 24000000 INFO @ Fri, 26 Jun 2020 09:08:59: 18000000 INFO @ Fri, 26 Jun 2020 09:09:01: 25000000 INFO @ Fri, 26 Jun 2020 09:09:02: 21000000 INFO @ Fri, 26 Jun 2020 09:09:07: 19000000 INFO @ Fri, 26 Jun 2020 09:09:09: 26000000 INFO @ Fri, 26 Jun 2020 09:09:11: 22000000 INFO @ Fri, 26 Jun 2020 09:09:15: 20000000 INFO @ Fri, 26 Jun 2020 09:09:17: 27000000 INFO @ Fri, 26 Jun 2020 09:09:19: 23000000 INFO @ Fri, 26 Jun 2020 09:09:23: 21000000 INFO @ Fri, 26 Jun 2020 09:09:24: 28000000 INFO @ Fri, 26 Jun 2020 09:09:28: 24000000 INFO @ Fri, 26 Jun 2020 09:09:30: 22000000 INFO @ Fri, 26 Jun 2020 09:09:32: 29000000 INFO @ Fri, 26 Jun 2020 09:09:36: 25000000 INFO @ Fri, 26 Jun 2020 09:09:38: 23000000 INFO @ Fri, 26 Jun 2020 09:09:40: 30000000 INFO @ Fri, 26 Jun 2020 09:09:44: 26000000 INFO @ Fri, 26 Jun 2020 09:09:46: 24000000 INFO @ Fri, 26 Jun 2020 09:09:48: 31000000 INFO @ Fri, 26 Jun 2020 09:09:52: 27000000 INFO @ Fri, 26 Jun 2020 09:09:54: 25000000 INFO @ Fri, 26 Jun 2020 09:09:57: 32000000 INFO @ Fri, 26 Jun 2020 09:10:01: 28000000 INFO @ Fri, 26 Jun 2020 09:10:02: 26000000 INFO @ Fri, 26 Jun 2020 09:10:05: 33000000 INFO @ Fri, 26 Jun 2020 09:10:09: 29000000 INFO @ Fri, 26 Jun 2020 09:10:10: 27000000 INFO @ Fri, 26 Jun 2020 09:10:14: 34000000 INFO @ Fri, 26 Jun 2020 09:10:17: 30000000 INFO @ Fri, 26 Jun 2020 09:10:18: 28000000 INFO @ Fri, 26 Jun 2020 09:10:22: 35000000 INFO @ Fri, 26 Jun 2020 09:10:25: 29000000 INFO @ Fri, 26 Jun 2020 09:10:26: 31000000 INFO @ Fri, 26 Jun 2020 09:10:30: 36000000 INFO @ Fri, 26 Jun 2020 09:10:33: 30000000 INFO @ Fri, 26 Jun 2020 09:10:34: 32000000 INFO @ Fri, 26 Jun 2020 09:10:38: 37000000 INFO @ Fri, 26 Jun 2020 09:10:41: 31000000 INFO @ Fri, 26 Jun 2020 09:10:43: 33000000 INFO @ Fri, 26 Jun 2020 09:10:47: 38000000 INFO @ Fri, 26 Jun 2020 09:10:48: 32000000 INFO @ Fri, 26 Jun 2020 09:10:51: 34000000 INFO @ Fri, 26 Jun 2020 09:10:56: 39000000 INFO @ Fri, 26 Jun 2020 09:10:56: 33000000 INFO @ Fri, 26 Jun 2020 09:10:59: 35000000 INFO @ Fri, 26 Jun 2020 09:11:04: 34000000 INFO @ Fri, 26 Jun 2020 09:11:04: 40000000 INFO @ Fri, 26 Jun 2020 09:11:07: 36000000 INFO @ Fri, 26 Jun 2020 09:11:11: 35000000 INFO @ Fri, 26 Jun 2020 09:11:12: 41000000 INFO @ Fri, 26 Jun 2020 09:11:15: 37000000 INFO @ Fri, 26 Jun 2020 09:11:18: 36000000 INFO @ Fri, 26 Jun 2020 09:11:21: 42000000 INFO @ Fri, 26 Jun 2020 09:11:24: 38000000 INFO @ Fri, 26 Jun 2020 09:11:26: 37000000 INFO @ Fri, 26 Jun 2020 09:11:30: 43000000 INFO @ Fri, 26 Jun 2020 09:11:33: 39000000 INFO @ Fri, 26 Jun 2020 09:11:33: 38000000 INFO @ Fri, 26 Jun 2020 09:11:38: 44000000 INFO @ Fri, 26 Jun 2020 09:11:41: 39000000 INFO @ Fri, 26 Jun 2020 09:11:41: 40000000 INFO @ Fri, 26 Jun 2020 09:11:46: 45000000 INFO @ Fri, 26 Jun 2020 09:11:48: 40000000 INFO @ Fri, 26 Jun 2020 09:11:49: 41000000 INFO @ Fri, 26 Jun 2020 09:11:53: 46000000 INFO @ Fri, 26 Jun 2020 09:11:56: 41000000 INFO @ Fri, 26 Jun 2020 09:11:58: 42000000 INFO @ Fri, 26 Jun 2020 09:12:02: 47000000 INFO @ Fri, 26 Jun 2020 09:12:04: 42000000 INFO @ Fri, 26 Jun 2020 09:12:06: 43000000 INFO @ Fri, 26 Jun 2020 09:12:09: 48000000 INFO @ Fri, 26 Jun 2020 09:12:11: 43000000 INFO @ Fri, 26 Jun 2020 09:12:15: 44000000 INFO @ Fri, 26 Jun 2020 09:12:17: 49000000 INFO @ Fri, 26 Jun 2020 09:12:19: 44000000 INFO @ Fri, 26 Jun 2020 09:12:23: 45000000 INFO @ Fri, 26 Jun 2020 09:12:25: 50000000 INFO @ Fri, 26 Jun 2020 09:12:26: 45000000 INFO @ Fri, 26 Jun 2020 09:12:32: 46000000 INFO @ Fri, 26 Jun 2020 09:12:33: 51000000 INFO @ Fri, 26 Jun 2020 09:12:34: 46000000 INFO @ Fri, 26 Jun 2020 09:12:40: 47000000 INFO @ Fri, 26 Jun 2020 09:12:41: 52000000 INFO @ Fri, 26 Jun 2020 09:12:41: 47000000 INFO @ Fri, 26 Jun 2020 09:12:48: 53000000 INFO @ Fri, 26 Jun 2020 09:12:49: 48000000 INFO @ Fri, 26 Jun 2020 09:12:49: 48000000 INFO @ Fri, 26 Jun 2020 09:12:56: 54000000 INFO @ Fri, 26 Jun 2020 09:12:58: 49000000 INFO @ Fri, 26 Jun 2020 09:12:59: 49000000 INFO @ Fri, 26 Jun 2020 09:13:04: 55000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 09:13:08: 50000000 INFO @ Fri, 26 Jun 2020 09:13:08: 50000000 INFO @ Fri, 26 Jun 2020 09:13:11: 56000000 INFO @ Fri, 26 Jun 2020 09:13:18: 51000000 INFO @ Fri, 26 Jun 2020 09:13:18: 51000000 INFO @ Fri, 26 Jun 2020 09:13:19: 57000000 INFO @ Fri, 26 Jun 2020 09:13:27: 58000000 INFO @ Fri, 26 Jun 2020 09:13:27: 52000000 INFO @ Fri, 26 Jun 2020 09:13:27: 52000000 INFO @ Fri, 26 Jun 2020 09:13:35: 59000000 INFO @ Fri, 26 Jun 2020 09:13:36: 53000000 INFO @ Fri, 26 Jun 2020 09:13:36: 53000000 INFO @ Fri, 26 Jun 2020 09:13:42: 60000000 INFO @ Fri, 26 Jun 2020 09:13:45: 54000000 INFO @ Fri, 26 Jun 2020 09:13:45: 54000000 INFO @ Fri, 26 Jun 2020 09:13:50: 61000000 INFO @ Fri, 26 Jun 2020 09:13:54: 55000000 INFO @ Fri, 26 Jun 2020 09:13:54: 55000000 INFO @ Fri, 26 Jun 2020 09:13:58: 62000000 INFO @ Fri, 26 Jun 2020 09:14:04: 56000000 INFO @ Fri, 26 Jun 2020 09:14:04: 56000000 INFO @ Fri, 26 Jun 2020 09:14:05: 63000000 INFO @ Fri, 26 Jun 2020 09:14:12: 64000000 INFO @ Fri, 26 Jun 2020 09:14:13: 57000000 INFO @ Fri, 26 Jun 2020 09:14:13: 57000000 INFO @ Fri, 26 Jun 2020 09:14:20: 65000000 INFO @ Fri, 26 Jun 2020 09:14:23: 58000000 INFO @ Fri, 26 Jun 2020 09:14:23: 58000000 INFO @ Fri, 26 Jun 2020 09:14:27: 66000000 INFO @ Fri, 26 Jun 2020 09:14:31: #1 tag size is determined as 101 bps INFO @ Fri, 26 Jun 2020 09:14:31: #1 tag size = 101 INFO @ Fri, 26 Jun 2020 09:14:31: #1 total tags in treatment: 32900207 INFO @ Fri, 26 Jun 2020 09:14:31: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:14:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:14:32: #1 tags after filtering in treatment: 28804695 INFO @ Fri, 26 Jun 2020 09:14:32: #1 Redundant rate of treatment: 0.12 INFO @ Fri, 26 Jun 2020 09:14:32: #1 finished! INFO @ Fri, 26 Jun 2020 09:14:32: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:14:32: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:14:32: 59000000 INFO @ Fri, 26 Jun 2020 09:14:32: 59000000 INFO @ Fri, 26 Jun 2020 09:14:34: #2 number of paired peaks: 123 WARNING @ Fri, 26 Jun 2020 09:14:34: Fewer paired peaks (123) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 123 pairs to build model! INFO @ Fri, 26 Jun 2020 09:14:34: start model_add_line... INFO @ Fri, 26 Jun 2020 09:14:34: start X-correlation... INFO @ Fri, 26 Jun 2020 09:14:34: end of X-cor INFO @ Fri, 26 Jun 2020 09:14:34: #2 finished! INFO @ Fri, 26 Jun 2020 09:14:34: #2 predicted fragment length is 109 bps INFO @ Fri, 26 Jun 2020 09:14:34: #2 alternative fragment length(s) may be 2,109,131,183,200 bps INFO @ Fri, 26 Jun 2020 09:14:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.05_model.r WARNING @ Fri, 26 Jun 2020 09:14:34: #2 Since the d (109) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 26 Jun 2020 09:14:34: #2 You may need to consider one of the other alternative d(s): 2,109,131,183,200 WARNING @ Fri, 26 Jun 2020 09:14:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 26 Jun 2020 09:14:34: #3 Call peaks... INFO @ Fri, 26 Jun 2020 09:14:34: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 26 Jun 2020 09:14:42: 60000000 INFO @ Fri, 26 Jun 2020 09:14:42: 60000000 BigWig に変換しました。 INFO @ Fri, 26 Jun 2020 09:14:51: 61000000 INFO @ Fri, 26 Jun 2020 09:14:52: 61000000 INFO @ Fri, 26 Jun 2020 09:15:02: 62000000 INFO @ Fri, 26 Jun 2020 09:15:02: 62000000 INFO @ Fri, 26 Jun 2020 09:15:11: 63000000 INFO @ Fri, 26 Jun 2020 09:15:11: 63000000 INFO @ Fri, 26 Jun 2020 09:15:20: 64000000 INFO @ Fri, 26 Jun 2020 09:15:20: 64000000 INFO @ Fri, 26 Jun 2020 09:15:27: #3 Call peaks for each chromosome... INFO @ Fri, 26 Jun 2020 09:15:27: 65000000 INFO @ Fri, 26 Jun 2020 09:15:29: 65000000 INFO @ Fri, 26 Jun 2020 09:15:35: 66000000 INFO @ Fri, 26 Jun 2020 09:15:37: 66000000 INFO @ Fri, 26 Jun 2020 09:15:39: #1 tag size is determined as 101 bps INFO @ Fri, 26 Jun 2020 09:15:39: #1 tag size = 101 INFO @ Fri, 26 Jun 2020 09:15:39: #1 total tags in treatment: 32900207 INFO @ Fri, 26 Jun 2020 09:15:39: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:15:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:15:40: #1 tags after filtering in treatment: 28804695 INFO @ Fri, 26 Jun 2020 09:15:40: #1 Redundant rate of treatment: 0.12 INFO @ Fri, 26 Jun 2020 09:15:40: #1 finished! INFO @ Fri, 26 Jun 2020 09:15:40: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:15:40: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:15:42: #1 tag size is determined as 101 bps INFO @ Fri, 26 Jun 2020 09:15:42: #1 tag size = 101 INFO @ Fri, 26 Jun 2020 09:15:42: #1 total tags in treatment: 32900207 INFO @ Fri, 26 Jun 2020 09:15:42: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 09:15:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 09:15:42: #2 number of paired peaks: 123 WARNING @ Fri, 26 Jun 2020 09:15:42: Fewer paired peaks (123) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 123 pairs to build model! INFO @ Fri, 26 Jun 2020 09:15:42: start model_add_line... INFO @ Fri, 26 Jun 2020 09:15:42: start X-correlation... INFO @ Fri, 26 Jun 2020 09:15:42: end of X-cor INFO @ Fri, 26 Jun 2020 09:15:42: #2 finished! INFO @ Fri, 26 Jun 2020 09:15:42: #2 predicted fragment length is 109 bps INFO @ Fri, 26 Jun 2020 09:15:42: #2 alternative fragment length(s) may be 2,109,131,183,200 bps INFO @ Fri, 26 Jun 2020 09:15:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.20_model.r WARNING @ Fri, 26 Jun 2020 09:15:42: #2 Since the d (109) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 26 Jun 2020 09:15:42: #2 You may need to consider one of the other alternative d(s): 2,109,131,183,200 WARNING @ Fri, 26 Jun 2020 09:15:42: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 26 Jun 2020 09:15:42: #3 Call peaks... INFO @ Fri, 26 Jun 2020 09:15:42: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 26 Jun 2020 09:15:42: #1 tags after filtering in treatment: 28804695 INFO @ Fri, 26 Jun 2020 09:15:42: #1 Redundant rate of treatment: 0.12 INFO @ Fri, 26 Jun 2020 09:15:42: #1 finished! INFO @ Fri, 26 Jun 2020 09:15:42: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 09:15:42: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 09:15:44: #2 number of paired peaks: 123 WARNING @ Fri, 26 Jun 2020 09:15:44: Fewer paired peaks (123) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 123 pairs to build model! INFO @ Fri, 26 Jun 2020 09:15:44: start model_add_line... INFO @ Fri, 26 Jun 2020 09:15:45: start X-correlation... INFO @ Fri, 26 Jun 2020 09:15:45: end of X-cor INFO @ Fri, 26 Jun 2020 09:15:45: #2 finished! INFO @ Fri, 26 Jun 2020 09:15:45: #2 predicted fragment length is 109 bps INFO @ Fri, 26 Jun 2020 09:15:45: #2 alternative fragment length(s) may be 2,109,131,183,200 bps INFO @ Fri, 26 Jun 2020 09:15:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.10_model.r WARNING @ Fri, 26 Jun 2020 09:15:45: #2 Since the d (109) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 26 Jun 2020 09:15:45: #2 You may need to consider one of the other alternative d(s): 2,109,131,183,200 WARNING @ Fri, 26 Jun 2020 09:15:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 26 Jun 2020 09:15:45: #3 Call peaks... INFO @ Fri, 26 Jun 2020 09:15:45: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 26 Jun 2020 09:15:52: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.05_peaks.xls INFO @ Fri, 26 Jun 2020 09:15:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.05_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 09:15:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.05_summits.bed INFO @ Fri, 26 Jun 2020 09:15:52: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (602 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:16:35: #3 Call peaks for each chromosome... INFO @ Fri, 26 Jun 2020 09:16:36: #3 Call peaks for each chromosome... INFO @ Fri, 26 Jun 2020 09:17:00: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.20_peaks.xls INFO @ Fri, 26 Jun 2020 09:17:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.20_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 09:17:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.20_summits.bed INFO @ Fri, 26 Jun 2020 09:17:00: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (245 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 09:17:01: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.10_peaks.xls INFO @ Fri, 26 Jun 2020 09:17:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.10_peaks.narrowPeak INFO @ Fri, 26 Jun 2020 09:17:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX1674103/SRX1674103.10_summits.bed INFO @ Fri, 26 Jun 2020 09:17:01: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (421 records, 4 fields): 3 millis CompletedMACS2peakCalling