Job ID = 9157379 sra ファイルのダウンロード中... Completed: 1777000K bytes transferred in 15 seconds (955234K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 74193295 spots for /home/okishinya/chipatlas/results/ce10/SRX1674089/SRR3320135.sra Written 74193295 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:10 74193295 reads; of these: 74193295 (100.00%) were unpaired; of these: 9006016 (12.14%) aligned 0 times 53665668 (72.33%) aligned exactly 1 time 11521611 (15.53%) aligned >1 times 87.86% overall alignment rate Time searching: 00:19:10 Overall time: 00:19:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 20192500 / 65187279 = 0.3098 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 12:09:20: # Command line: callpeak -t SRX1674089.bam -f BAM -g ce -n SRX1674089.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1674089.20 # format = BAM # ChIP-seq file = ['SRX1674089.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 12:09:20: # Command line: callpeak -t SRX1674089.bam -f BAM -g ce -n SRX1674089.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1674089.10 # format = BAM # ChIP-seq file = ['SRX1674089.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 12:09:20: # Command line: callpeak -t SRX1674089.bam -f BAM -g ce -n SRX1674089.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1674089.05 # format = BAM # ChIP-seq file = ['SRX1674089.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 12:09:20: #1 read tag files... INFO @ Tue, 27 Jun 2017 12:09:20: #1 read tag files... INFO @ Tue, 27 Jun 2017 12:09:20: #1 read tag files... INFO @ Tue, 27 Jun 2017 12:09:20: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 12:09:20: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 12:09:20: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 12:09:27: 1000000 INFO @ Tue, 27 Jun 2017 12:09:27: 1000000 INFO @ Tue, 27 Jun 2017 12:09:27: 1000000 INFO @ Tue, 27 Jun 2017 12:09:33: 2000000 INFO @ Tue, 27 Jun 2017 12:09:34: 2000000 INFO @ Tue, 27 Jun 2017 12:09:34: 2000000 INFO @ Tue, 27 Jun 2017 12:09:40: 3000000 INFO @ Tue, 27 Jun 2017 12:09:40: 3000000 INFO @ Tue, 27 Jun 2017 12:09:40: 3000000 INFO @ Tue, 27 Jun 2017 12:09:46: 4000000 INFO @ Tue, 27 Jun 2017 12:09:47: 4000000 INFO @ Tue, 27 Jun 2017 12:09:47: 4000000 INFO @ Tue, 27 Jun 2017 12:09:53: 5000000 INFO @ Tue, 27 Jun 2017 12:09:54: 5000000 INFO @ Tue, 27 Jun 2017 12:09:54: 5000000 INFO @ Tue, 27 Jun 2017 12:09:59: 6000000 INFO @ Tue, 27 Jun 2017 12:10:00: 6000000 INFO @ Tue, 27 Jun 2017 12:10:00: 6000000 INFO @ Tue, 27 Jun 2017 12:10:05: 7000000 INFO @ Tue, 27 Jun 2017 12:10:06: 7000000 INFO @ Tue, 27 Jun 2017 12:10:07: 7000000 INFO @ Tue, 27 Jun 2017 12:10:12: 8000000 INFO @ Tue, 27 Jun 2017 12:10:14: 8000000 INFO @ Tue, 27 Jun 2017 12:10:14: 8000000 INFO @ Tue, 27 Jun 2017 12:10:19: 9000000 INFO @ Tue, 27 Jun 2017 12:10:20: 9000000 INFO @ Tue, 27 Jun 2017 12:10:21: 9000000 INFO @ Tue, 27 Jun 2017 12:10:26: 10000000 INFO @ Tue, 27 Jun 2017 12:10:26: 10000000 INFO @ Tue, 27 Jun 2017 12:10:28: 10000000 INFO @ Tue, 27 Jun 2017 12:10:33: 11000000 INFO @ Tue, 27 Jun 2017 12:10:33: 11000000 INFO @ Tue, 27 Jun 2017 12:10:35: 11000000 INFO @ Tue, 27 Jun 2017 12:10:40: 12000000 INFO @ Tue, 27 Jun 2017 12:10:40: 12000000 INFO @ Tue, 27 Jun 2017 12:10:41: 12000000 INFO @ Tue, 27 Jun 2017 12:10:46: 13000000 INFO @ Tue, 27 Jun 2017 12:10:47: 13000000 INFO @ Tue, 27 Jun 2017 12:10:48: 13000000 INFO @ Tue, 27 Jun 2017 12:10:53: 14000000 INFO @ Tue, 27 Jun 2017 12:10:54: 14000000 INFO @ Tue, 27 Jun 2017 12:10:55: 14000000 INFO @ Tue, 27 Jun 2017 12:10:59: 15000000 INFO @ Tue, 27 Jun 2017 12:11:01: 15000000 INFO @ Tue, 27 Jun 2017 12:11:02: 15000000 INFO @ Tue, 27 Jun 2017 12:11:07: 16000000 INFO @ Tue, 27 Jun 2017 12:11:08: 16000000 INFO @ Tue, 27 Jun 2017 12:11:10: 16000000 INFO @ Tue, 27 Jun 2017 12:11:16: 17000000 INFO @ Tue, 27 Jun 2017 12:11:16: 17000000 INFO @ Tue, 27 Jun 2017 12:11:17: 17000000 INFO @ Tue, 27 Jun 2017 12:11:23: 18000000 INFO @ Tue, 27 Jun 2017 12:11:23: 18000000 INFO @ Tue, 27 Jun 2017 12:11:24: 18000000 INFO @ Tue, 27 Jun 2017 12:11:31: 19000000 INFO @ Tue, 27 Jun 2017 12:11:31: 19000000 INFO @ Tue, 27 Jun 2017 12:11:32: 19000000 INFO @ Tue, 27 Jun 2017 12:11:38: 20000000 INFO @ Tue, 27 Jun 2017 12:11:39: 20000000 INFO @ Tue, 27 Jun 2017 12:11:39: 20000000 INFO @ Tue, 27 Jun 2017 12:11:45: 21000000 INFO @ Tue, 27 Jun 2017 12:11:46: 21000000 INFO @ Tue, 27 Jun 2017 12:11:46: 21000000 INFO @ Tue, 27 Jun 2017 12:11:53: 22000000 INFO @ Tue, 27 Jun 2017 12:11:54: 22000000 INFO @ Tue, 27 Jun 2017 12:11:54: 22000000 INFO @ Tue, 27 Jun 2017 12:12:00: 23000000 INFO @ Tue, 27 Jun 2017 12:12:01: 23000000 INFO @ Tue, 27 Jun 2017 12:12:01: 23000000 INFO @ Tue, 27 Jun 2017 12:12:08: 24000000 INFO @ Tue, 27 Jun 2017 12:12:08: 24000000 INFO @ Tue, 27 Jun 2017 12:12:10: 24000000 INFO @ Tue, 27 Jun 2017 12:12:16: 25000000 INFO @ Tue, 27 Jun 2017 12:12:16: 25000000 INFO @ Tue, 27 Jun 2017 12:12:18: 25000000 INFO @ Tue, 27 Jun 2017 12:12:24: 26000000 INFO @ Tue, 27 Jun 2017 12:12:24: 26000000 INFO @ Tue, 27 Jun 2017 12:12:27: 26000000 INFO @ Tue, 27 Jun 2017 12:12:32: 27000000 INFO @ Tue, 27 Jun 2017 12:12:32: 27000000 INFO @ Tue, 27 Jun 2017 12:12:36: 27000000 INFO @ Tue, 27 Jun 2017 12:12:39: 28000000 INFO @ Tue, 27 Jun 2017 12:12:40: 28000000 INFO @ Tue, 27 Jun 2017 12:12:44: 28000000 INFO @ Tue, 27 Jun 2017 12:12:47: 29000000 INFO @ Tue, 27 Jun 2017 12:12:47: 29000000 INFO @ Tue, 27 Jun 2017 12:12:53: 29000000 INFO @ Tue, 27 Jun 2017 12:12:55: 30000000 INFO @ Tue, 27 Jun 2017 12:12:55: 30000000 INFO @ Tue, 27 Jun 2017 12:13:02: 30000000 INFO @ Tue, 27 Jun 2017 12:13:03: 31000000 INFO @ Tue, 27 Jun 2017 12:13:03: 31000000 INFO @ Tue, 27 Jun 2017 12:13:11: 31000000 INFO @ Tue, 27 Jun 2017 12:13:11: 32000000 INFO @ Tue, 27 Jun 2017 12:13:11: 32000000 INFO @ Tue, 27 Jun 2017 12:13:19: 33000000 INFO @ Tue, 27 Jun 2017 12:13:19: 33000000 INFO @ Tue, 27 Jun 2017 12:13:20: 32000000 INFO @ Tue, 27 Jun 2017 12:13:26: 34000000 INFO @ Tue, 27 Jun 2017 12:13:27: 34000000 INFO @ Tue, 27 Jun 2017 12:13:28: 33000000 INFO @ Tue, 27 Jun 2017 12:13:34: 35000000 INFO @ Tue, 27 Jun 2017 12:13:34: 35000000 INFO @ Tue, 27 Jun 2017 12:13:37: 34000000 INFO @ Tue, 27 Jun 2017 12:13:42: 36000000 INFO @ Tue, 27 Jun 2017 12:13:42: 36000000 INFO @ Tue, 27 Jun 2017 12:13:46: 35000000 INFO @ Tue, 27 Jun 2017 12:13:50: 37000000 INFO @ Tue, 27 Jun 2017 12:13:50: 37000000 INFO @ Tue, 27 Jun 2017 12:13:55: 36000000 INFO @ Tue, 27 Jun 2017 12:13:57: 38000000 INFO @ Tue, 27 Jun 2017 12:13:58: 38000000 INFO @ Tue, 27 Jun 2017 12:14:03: 37000000 INFO @ Tue, 27 Jun 2017 12:14:05: 39000000 INFO @ Tue, 27 Jun 2017 12:14:05: 39000000 INFO @ Tue, 27 Jun 2017 12:14:12: 38000000 INFO @ Tue, 27 Jun 2017 12:14:13: 40000000 INFO @ Tue, 27 Jun 2017 12:14:13: 40000000 INFO @ Tue, 27 Jun 2017 12:14:21: 39000000 INFO @ Tue, 27 Jun 2017 12:14:21: 41000000 INFO @ Tue, 27 Jun 2017 12:14:21: 41000000 INFO @ Tue, 27 Jun 2017 12:14:29: 42000000 INFO @ Tue, 27 Jun 2017 12:14:29: 42000000 INFO @ Tue, 27 Jun 2017 12:14:29: 40000000 INFO @ Tue, 27 Jun 2017 12:14:36: 43000000 INFO @ Tue, 27 Jun 2017 12:14:37: 43000000 INFO @ Tue, 27 Jun 2017 12:14:38: 41000000 INFO @ Tue, 27 Jun 2017 12:14:42: 44000000 INFO @ Tue, 27 Jun 2017 12:14:45: 44000000 INFO @ Tue, 27 Jun 2017 12:14:46: 42000000 INFO @ Tue, 27 Jun 2017 12:14:49: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 12:14:49: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 12:14:49: #1 total tags in treatment: 44994779 INFO @ Tue, 27 Jun 2017 12:14:49: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 12:14:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 12:14:50: #1 tags after filtering in treatment: 44994779 INFO @ Tue, 27 Jun 2017 12:14:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 12:14:50: #1 finished! INFO @ Tue, 27 Jun 2017 12:14:50: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 12:14:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 12:14:52: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 12:14:52: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 12:14:52: #1 total tags in treatment: 44994779 INFO @ Tue, 27 Jun 2017 12:14:52: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 12:14:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 12:14:53: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 12:14:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 12:14:53: Process for pairing-model is terminated! cat: SRX1674089.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1674089.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1674089.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1674089.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 12:14:53: #1 tags after filtering in treatment: 44994779 INFO @ Tue, 27 Jun 2017 12:14:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 12:14:53: #1 finished! INFO @ Tue, 27 Jun 2017 12:14:53: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 12:14:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 12:14:53: 43000000 INFO @ Tue, 27 Jun 2017 12:14:56: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 12:14:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 12:14:56: Process for pairing-model is terminated! cat: SRX1674089.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1674089.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1674089.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1674089.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 12:15:00: 44000000 INFO @ Tue, 27 Jun 2017 12:15:06: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 12:15:06: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 12:15:06: #1 total tags in treatment: 44994779 INFO @ Tue, 27 Jun 2017 12:15:06: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 12:15:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 12:15:07: #1 tags after filtering in treatment: 44994779 INFO @ Tue, 27 Jun 2017 12:15:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 12:15:07: #1 finished! INFO @ Tue, 27 Jun 2017 12:15:07: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 12:15:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 12:15:10: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 12:15:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 12:15:10: Process for pairing-model is terminated! cat: SRX1674089.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1674089.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1674089.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1674089.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。