Job ID = 9157377 sra ファイルのダウンロード中... Completed: 1422194K bytes transferred in 13 seconds (890049K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 66061416 spots for /home/okishinya/chipatlas/results/ce10/SRX1674087/SRR3320133.sra Written 66061416 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:31 66061416 reads; of these: 66061416 (100.00%) were unpaired; of these: 13425101 (20.32%) aligned 0 times 45563541 (68.97%) aligned exactly 1 time 7072774 (10.71%) aligned >1 times 79.68% overall alignment rate Time searching: 00:15:31 Overall time: 00:15:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 18032356 / 52636315 = 0.3426 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 11:59:08: # Command line: callpeak -t SRX1674087.bam -f BAM -g ce -n SRX1674087.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1674087.10 # format = BAM # ChIP-seq file = ['SRX1674087.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:59:08: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:59:08: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:59:08: # Command line: callpeak -t SRX1674087.bam -f BAM -g ce -n SRX1674087.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1674087.20 # format = BAM # ChIP-seq file = ['SRX1674087.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:59:08: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:59:08: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:59:08: # Command line: callpeak -t SRX1674087.bam -f BAM -g ce -n SRX1674087.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1674087.05 # format = BAM # ChIP-seq file = ['SRX1674087.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:59:08: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:59:08: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:59:14: 1000000 INFO @ Tue, 27 Jun 2017 11:59:15: 1000000 INFO @ Tue, 27 Jun 2017 11:59:15: 1000000 INFO @ Tue, 27 Jun 2017 11:59:21: 2000000 INFO @ Tue, 27 Jun 2017 11:59:23: 2000000 INFO @ Tue, 27 Jun 2017 11:59:23: 2000000 INFO @ Tue, 27 Jun 2017 11:59:28: 3000000 INFO @ Tue, 27 Jun 2017 11:59:31: 3000000 INFO @ Tue, 27 Jun 2017 11:59:31: 3000000 INFO @ Tue, 27 Jun 2017 11:59:35: 4000000 INFO @ Tue, 27 Jun 2017 11:59:38: 4000000 INFO @ Tue, 27 Jun 2017 11:59:38: 4000000 INFO @ Tue, 27 Jun 2017 11:59:42: 5000000 INFO @ Tue, 27 Jun 2017 11:59:46: 5000000 INFO @ Tue, 27 Jun 2017 11:59:46: 5000000 INFO @ Tue, 27 Jun 2017 11:59:49: 6000000 INFO @ Tue, 27 Jun 2017 11:59:53: 6000000 INFO @ Tue, 27 Jun 2017 11:59:53: 6000000 INFO @ Tue, 27 Jun 2017 11:59:55: 7000000 INFO @ Tue, 27 Jun 2017 12:00:01: 7000000 INFO @ Tue, 27 Jun 2017 12:00:01: 7000000 INFO @ Tue, 27 Jun 2017 12:00:02: 8000000 INFO @ Tue, 27 Jun 2017 12:00:09: 8000000 INFO @ Tue, 27 Jun 2017 12:00:09: 8000000 INFO @ Tue, 27 Jun 2017 12:00:09: 9000000 INFO @ Tue, 27 Jun 2017 12:00:16: 10000000 INFO @ Tue, 27 Jun 2017 12:00:16: 9000000 INFO @ Tue, 27 Jun 2017 12:00:16: 9000000 INFO @ Tue, 27 Jun 2017 12:00:23: 11000000 INFO @ Tue, 27 Jun 2017 12:00:24: 10000000 INFO @ Tue, 27 Jun 2017 12:00:24: 10000000 INFO @ Tue, 27 Jun 2017 12:00:29: 12000000 INFO @ Tue, 27 Jun 2017 12:00:31: 11000000 INFO @ Tue, 27 Jun 2017 12:00:31: 11000000 INFO @ Tue, 27 Jun 2017 12:00:36: 13000000 INFO @ Tue, 27 Jun 2017 12:00:39: 12000000 INFO @ Tue, 27 Jun 2017 12:00:39: 12000000 INFO @ Tue, 27 Jun 2017 12:00:43: 14000000 INFO @ Tue, 27 Jun 2017 12:00:46: 13000000 INFO @ Tue, 27 Jun 2017 12:00:46: 13000000 INFO @ Tue, 27 Jun 2017 12:00:49: 15000000 INFO @ Tue, 27 Jun 2017 12:00:54: 14000000 INFO @ Tue, 27 Jun 2017 12:00:54: 14000000 INFO @ Tue, 27 Jun 2017 12:00:56: 16000000 INFO @ Tue, 27 Jun 2017 12:01:01: 15000000 INFO @ Tue, 27 Jun 2017 12:01:01: 15000000 INFO @ Tue, 27 Jun 2017 12:01:03: 17000000 INFO @ Tue, 27 Jun 2017 12:01:09: 16000000 INFO @ Tue, 27 Jun 2017 12:01:09: 16000000 INFO @ Tue, 27 Jun 2017 12:01:09: 18000000 INFO @ Tue, 27 Jun 2017 12:01:16: 17000000 INFO @ Tue, 27 Jun 2017 12:01:16: 17000000 INFO @ Tue, 27 Jun 2017 12:01:16: 19000000 INFO @ Tue, 27 Jun 2017 12:01:23: 20000000 INFO @ Tue, 27 Jun 2017 12:01:23: 18000000 INFO @ Tue, 27 Jun 2017 12:01:23: 18000000 INFO @ Tue, 27 Jun 2017 12:01:29: 21000000 INFO @ Tue, 27 Jun 2017 12:01:30: 19000000 INFO @ Tue, 27 Jun 2017 12:01:30: 19000000 INFO @ Tue, 27 Jun 2017 12:01:36: 22000000 INFO @ Tue, 27 Jun 2017 12:01:37: 20000000 INFO @ Tue, 27 Jun 2017 12:01:37: 20000000 INFO @ Tue, 27 Jun 2017 12:01:43: 23000000 INFO @ Tue, 27 Jun 2017 12:01:45: 21000000 INFO @ Tue, 27 Jun 2017 12:01:45: 21000000 INFO @ Tue, 27 Jun 2017 12:01:50: 24000000 INFO @ Tue, 27 Jun 2017 12:01:53: 22000000 INFO @ Tue, 27 Jun 2017 12:01:53: 22000000 INFO @ Tue, 27 Jun 2017 12:01:57: 25000000 INFO @ Tue, 27 Jun 2017 12:02:02: 23000000 INFO @ Tue, 27 Jun 2017 12:02:02: 23000000 INFO @ Tue, 27 Jun 2017 12:02:04: 26000000 INFO @ Tue, 27 Jun 2017 12:02:11: 24000000 INFO @ Tue, 27 Jun 2017 12:02:11: 24000000 INFO @ Tue, 27 Jun 2017 12:02:11: 27000000 INFO @ Tue, 27 Jun 2017 12:02:18: 28000000 INFO @ Tue, 27 Jun 2017 12:02:20: 25000000 INFO @ Tue, 27 Jun 2017 12:02:20: 25000000 INFO @ Tue, 27 Jun 2017 12:02:25: 29000000 INFO @ Tue, 27 Jun 2017 12:02:28: 26000000 INFO @ Tue, 27 Jun 2017 12:02:28: 26000000 INFO @ Tue, 27 Jun 2017 12:02:32: 30000000 INFO @ Tue, 27 Jun 2017 12:02:35: 27000000 INFO @ Tue, 27 Jun 2017 12:02:35: 27000000 INFO @ Tue, 27 Jun 2017 12:02:39: 31000000 INFO @ Tue, 27 Jun 2017 12:02:43: 28000000 INFO @ Tue, 27 Jun 2017 12:02:43: 28000000 INFO @ Tue, 27 Jun 2017 12:02:45: 32000000 INFO @ Tue, 27 Jun 2017 12:02:50: 29000000 INFO @ Tue, 27 Jun 2017 12:02:50: 29000000 INFO @ Tue, 27 Jun 2017 12:02:52: 33000000 INFO @ Tue, 27 Jun 2017 12:02:57: 30000000 INFO @ Tue, 27 Jun 2017 12:02:57: 30000000 INFO @ Tue, 27 Jun 2017 12:02:58: 34000000 INFO @ Tue, 27 Jun 2017 12:03:03: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 12:03:03: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 12:03:03: #1 total tags in treatment: 34603959 INFO @ Tue, 27 Jun 2017 12:03:03: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 12:03:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 12:03:03: #1 tags after filtering in treatment: 34603959 INFO @ Tue, 27 Jun 2017 12:03:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 12:03:03: #1 finished! INFO @ Tue, 27 Jun 2017 12:03:03: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 12:03:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 12:03:04: 31000000 INFO @ Tue, 27 Jun 2017 12:03:04: 31000000 INFO @ Tue, 27 Jun 2017 12:03:06: #2 number of paired peaks: 13 WARNING @ Tue, 27 Jun 2017 12:03:06: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 12:03:06: Process for pairing-model is terminated! cat: SRX1674087.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1674087.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1674087.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1674087.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 12:03:11: 32000000 INFO @ Tue, 27 Jun 2017 12:03:11: 32000000 INFO @ Tue, 27 Jun 2017 12:03:18: 33000000 INFO @ Tue, 27 Jun 2017 12:03:18: 33000000 INFO @ Tue, 27 Jun 2017 12:03:25: 34000000 INFO @ Tue, 27 Jun 2017 12:03:25: 34000000 INFO @ Tue, 27 Jun 2017 12:03:30: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 12:03:30: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 12:03:30: #1 total tags in treatment: 34603959 INFO @ Tue, 27 Jun 2017 12:03:30: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 12:03:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 12:03:30: #1 tag size is determined as 51 bps INFO @ Tue, 27 Jun 2017 12:03:30: #1 tag size = 51 INFO @ Tue, 27 Jun 2017 12:03:30: #1 total tags in treatment: 34603959 INFO @ Tue, 27 Jun 2017 12:03:30: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 12:03:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 12:03:31: #1 tags after filtering in treatment: 34603959 INFO @ Tue, 27 Jun 2017 12:03:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 12:03:31: #1 finished! INFO @ Tue, 27 Jun 2017 12:03:31: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 12:03:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 12:03:31: #1 tags after filtering in treatment: 34603959 INFO @ Tue, 27 Jun 2017 12:03:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 12:03:31: #1 finished! INFO @ Tue, 27 Jun 2017 12:03:31: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 12:03:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 12:03:33: #2 number of paired peaks: 13 WARNING @ Tue, 27 Jun 2017 12:03:33: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 12:03:33: Process for pairing-model is terminated! cat: SRX1674087.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1674087.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1674087.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1674087.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 12:03:33: #2 number of paired peaks: 13 WARNING @ Tue, 27 Jun 2017 12:03:33: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 12:03:33: Process for pairing-model is terminated! cat: SRX1674087.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1674087.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1674087.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1674087.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。