Job ID = 2589396 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 5,027,684 reads read : 5,027,684 reads written : 5,027,684 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR494575.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:06 5027684 reads; of these: 5027684 (100.00%) were unpaired; of these: 85160 (1.69%) aligned 0 times 3920925 (77.99%) aligned exactly 1 time 1021599 (20.32%) aligned >1 times 98.31% overall alignment rate Time searching: 00:01:06 Overall time: 00:01:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 551035 / 4942524 = 0.1115 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 17:36:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:36:14: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:36:14: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:36:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:36:15: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:36:15: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:36:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:36:16: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:36:16: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:36:24: 1000000 INFO @ Mon, 12 Aug 2019 17:36:24: 1000000 INFO @ Mon, 12 Aug 2019 17:36:25: 1000000 INFO @ Mon, 12 Aug 2019 17:36:32: 2000000 INFO @ Mon, 12 Aug 2019 17:36:34: 2000000 INFO @ Mon, 12 Aug 2019 17:36:35: 2000000 INFO @ Mon, 12 Aug 2019 17:36:39: 3000000 INFO @ Mon, 12 Aug 2019 17:36:43: 3000000 INFO @ Mon, 12 Aug 2019 17:36:44: 3000000 INFO @ Mon, 12 Aug 2019 17:36:47: 4000000 INFO @ Mon, 12 Aug 2019 17:36:50: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:36:50: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:36:50: #1 total tags in treatment: 4391489 INFO @ Mon, 12 Aug 2019 17:36:50: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:36:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:36:50: #1 tags after filtering in treatment: 4391489 INFO @ Mon, 12 Aug 2019 17:36:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:36:50: #1 finished! INFO @ Mon, 12 Aug 2019 17:36:50: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:36:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:36:51: #2 number of paired peaks: 573 WARNING @ Mon, 12 Aug 2019 17:36:51: Fewer paired peaks (573) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 573 pairs to build model! INFO @ Mon, 12 Aug 2019 17:36:51: start model_add_line... INFO @ Mon, 12 Aug 2019 17:36:51: start X-correlation... INFO @ Mon, 12 Aug 2019 17:36:51: end of X-cor INFO @ Mon, 12 Aug 2019 17:36:51: #2 finished! INFO @ Mon, 12 Aug 2019 17:36:51: #2 predicted fragment length is 31 bps INFO @ Mon, 12 Aug 2019 17:36:51: #2 alternative fragment length(s) may be 2,31,592 bps INFO @ Mon, 12 Aug 2019 17:36:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.20_model.r WARNING @ Mon, 12 Aug 2019 17:36:51: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:36:51: #2 You may need to consider one of the other alternative d(s): 2,31,592 WARNING @ Mon, 12 Aug 2019 17:36:51: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:36:51: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:36:51: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:36:52: 4000000 INFO @ Mon, 12 Aug 2019 17:36:52: 4000000 INFO @ Mon, 12 Aug 2019 17:36:55: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:36:55: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:36:55: #1 total tags in treatment: 4391489 INFO @ Mon, 12 Aug 2019 17:36:55: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:36:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:36:55: #1 tags after filtering in treatment: 4391489 INFO @ Mon, 12 Aug 2019 17:36:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:36:55: #1 finished! INFO @ Mon, 12 Aug 2019 17:36:55: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:36:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:36:56: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:36:56: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:36:56: #1 total tags in treatment: 4391489 INFO @ Mon, 12 Aug 2019 17:36:56: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:36:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:36:56: #2 number of paired peaks: 573 WARNING @ Mon, 12 Aug 2019 17:36:56: Fewer paired peaks (573) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 573 pairs to build model! INFO @ Mon, 12 Aug 2019 17:36:56: start model_add_line... INFO @ Mon, 12 Aug 2019 17:36:56: #1 tags after filtering in treatment: 4391489 INFO @ Mon, 12 Aug 2019 17:36:56: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:36:56: #1 finished! INFO @ Mon, 12 Aug 2019 17:36:56: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:36:56: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:36:56: start X-correlation... INFO @ Mon, 12 Aug 2019 17:36:56: end of X-cor INFO @ Mon, 12 Aug 2019 17:36:56: #2 finished! INFO @ Mon, 12 Aug 2019 17:36:56: #2 predicted fragment length is 31 bps INFO @ Mon, 12 Aug 2019 17:36:56: #2 alternative fragment length(s) may be 2,31,592 bps INFO @ Mon, 12 Aug 2019 17:36:56: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.10_model.r WARNING @ Mon, 12 Aug 2019 17:36:56: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:36:56: #2 You may need to consider one of the other alternative d(s): 2,31,592 WARNING @ Mon, 12 Aug 2019 17:36:56: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:36:56: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:36:56: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:36:56: #2 number of paired peaks: 573 WARNING @ Mon, 12 Aug 2019 17:36:56: Fewer paired peaks (573) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 573 pairs to build model! INFO @ Mon, 12 Aug 2019 17:36:56: start model_add_line... INFO @ Mon, 12 Aug 2019 17:36:56: start X-correlation... INFO @ Mon, 12 Aug 2019 17:36:56: end of X-cor INFO @ Mon, 12 Aug 2019 17:36:56: #2 finished! INFO @ Mon, 12 Aug 2019 17:36:56: #2 predicted fragment length is 31 bps INFO @ Mon, 12 Aug 2019 17:36:56: #2 alternative fragment length(s) may be 2,31,592 bps INFO @ Mon, 12 Aug 2019 17:36:56: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.05_model.r WARNING @ Mon, 12 Aug 2019 17:36:56: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:36:56: #2 You may need to consider one of the other alternative d(s): 2,31,592 WARNING @ Mon, 12 Aug 2019 17:36:56: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:36:56: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:36:56: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:37:03: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:37:09: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:37:09: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:37:09: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.20_peaks.xls INFO @ Mon, 12 Aug 2019 17:37:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:37:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.20_summits.bed INFO @ Mon, 12 Aug 2019 17:37:09: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (88 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:37:15: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.10_peaks.xls INFO @ Mon, 12 Aug 2019 17:37:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:37:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.10_summits.bed INFO @ Mon, 12 Aug 2019 17:37:15: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (280 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:37:15: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.05_peaks.xls INFO @ Mon, 12 Aug 2019 17:37:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:37:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX146414/SRX146414.05_summits.bed INFO @ Mon, 12 Aug 2019 17:37:15: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (583 records, 4 fields): 4 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。