Job ID = 9025498 sra ファイルのダウンロード中... Completed: 28884K bytes transferred in 3 seconds (72217K bits/sec), in 1 file, 2 directories. % Total % Received % Xferd Average Speed Time Time Time Current Dload Upload Total Spent Left Speed 0 0 0 0 0 0 0 0 --:--:-- 0:00:06 --:--:-- 0 100 7663 0 7663 0 0 1106 0 --:--:-- 0:00:06 --:--:-- 11825 100 30318 0 30318 0 0 3828 0 --:--:-- 0:00:07 --:--:-- 18441 100 42735 0 42735 0 0 5178 0 --:--:-- 0:00:08 --:--:-- 21627 sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 1167863 spots for /home/okishinya/chipatlas/results/ce10/SRX1388747/SRR2832463.sra Written 1167863 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:21 1167863 reads; of these: 1167863 (100.00%) were unpaired; of these: 299736 (25.67%) aligned 0 times 673908 (57.70%) aligned exactly 1 time 194219 (16.63%) aligned >1 times 74.33% overall alignment rate Time searching: 00:00:21 Overall time: 00:00:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 34204 / 868127 = 0.0394 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 03 Jun 2017 04:08:09: # Command line: callpeak -t SRX1388747.bam -f BAM -g ce -n SRX1388747.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1388747.05 # format = BAM # ChIP-seq file = ['SRX1388747.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Sat, 03 Jun 2017 04:08:09: #1 read tag files... INFO @ Sat, 03 Jun 2017 04:08:09: #1 read treatment tags... INFO @ Sat, 03 Jun 2017 04:08:09: # Command line: callpeak -t SRX1388747.bam -f BAM -g ce -n SRX1388747.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1388747.20 # format = BAM # ChIP-seq file = ['SRX1388747.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Sat, 03 Jun 2017 04:08:09: #1 read tag files... INFO @ Sat, 03 Jun 2017 04:08:09: #1 read treatment tags... INFO @ Sat, 03 Jun 2017 04:08:09: # Command line: callpeak -t SRX1388747.bam -f BAM -g ce -n SRX1388747.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1388747.10 # format = BAM # ChIP-seq file = ['SRX1388747.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Sat, 03 Jun 2017 04:08:09: #1 read tag files... INFO @ Sat, 03 Jun 2017 04:08:09: #1 read treatment tags... INFO @ Sat, 03 Jun 2017 04:08:14: #1 tag size is determined as 50 bps INFO @ Sat, 03 Jun 2017 04:08:14: #1 tag size = 50 INFO @ Sat, 03 Jun 2017 04:08:14: #1 total tags in treatment: 833923 INFO @ Sat, 03 Jun 2017 04:08:14: #1 user defined the maximum tags... INFO @ Sat, 03 Jun 2017 04:08:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Jun 2017 04:08:14: #1 tag size is determined as 50 bps INFO @ Sat, 03 Jun 2017 04:08:14: #1 tag size = 50 INFO @ Sat, 03 Jun 2017 04:08:14: #1 total tags in treatment: 833923 INFO @ Sat, 03 Jun 2017 04:08:14: #1 user defined the maximum tags... INFO @ Sat, 03 Jun 2017 04:08:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Jun 2017 04:08:14: #1 tags after filtering in treatment: 833794 INFO @ Sat, 03 Jun 2017 04:08:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Jun 2017 04:08:14: #1 finished! INFO @ Sat, 03 Jun 2017 04:08:14: #2 Build Peak Model... INFO @ Sat, 03 Jun 2017 04:08:14: #1 tags after filtering in treatment: 833794 INFO @ Sat, 03 Jun 2017 04:08:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Jun 2017 04:08:14: #1 finished! INFO @ Sat, 03 Jun 2017 04:08:14: #2 Build Peak Model... INFO @ Sat, 03 Jun 2017 04:08:14: #1 tag size is determined as 50 bps INFO @ Sat, 03 Jun 2017 04:08:14: #1 tag size = 50 INFO @ Sat, 03 Jun 2017 04:08:14: #1 total tags in treatment: 833923 INFO @ Sat, 03 Jun 2017 04:08:14: #1 user defined the maximum tags... INFO @ Sat, 03 Jun 2017 04:08:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Jun 2017 04:08:15: #2 number of paired peaks: 741 WARNING @ Sat, 03 Jun 2017 04:08:15: Fewer paired peaks (741) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 741 pairs to build model! INFO @ Sat, 03 Jun 2017 04:08:15: start model_add_line... INFO @ Sat, 03 Jun 2017 04:08:15: #1 tags after filtering in treatment: 833794 INFO @ Sat, 03 Jun 2017 04:08:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 03 Jun 2017 04:08:15: #1 finished! INFO @ Sat, 03 Jun 2017 04:08:15: #2 Build Peak Model... INFO @ Sat, 03 Jun 2017 04:08:15: #2 number of paired peaks: 741 WARNING @ Sat, 03 Jun 2017 04:08:15: Fewer paired peaks (741) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 741 pairs to build model! INFO @ Sat, 03 Jun 2017 04:08:15: start model_add_line... INFO @ Sat, 03 Jun 2017 04:08:15: #2 number of paired peaks: 741 WARNING @ Sat, 03 Jun 2017 04:08:15: Fewer paired peaks (741) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 741 pairs to build model! INFO @ Sat, 03 Jun 2017 04:08:15: start model_add_line... INFO @ Sat, 03 Jun 2017 04:08:15: start X-correlation... INFO @ Sat, 03 Jun 2017 04:08:15: end of X-cor INFO @ Sat, 03 Jun 2017 04:08:15: #2 finished! INFO @ Sat, 03 Jun 2017 04:08:15: #2 predicted fragment length is 52 bps INFO @ Sat, 03 Jun 2017 04:08:15: #2 alternative fragment length(s) may be 52,131,530 bps INFO @ Sat, 03 Jun 2017 04:08:15: #2.2 Generate R script for model : SRX1388747.10_model.r WARNING @ Sat, 03 Jun 2017 04:08:15: #2 Since the d (52) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Jun 2017 04:08:15: #2 You may need to consider one of the other alternative d(s): 52,131,530 WARNING @ Sat, 03 Jun 2017 04:08:15: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Jun 2017 04:08:15: #3 Call peaks... INFO @ Sat, 03 Jun 2017 04:08:15: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Jun 2017 04:08:15: start X-correlation... INFO @ Sat, 03 Jun 2017 04:08:15: end of X-cor INFO @ Sat, 03 Jun 2017 04:08:15: #2 finished! INFO @ Sat, 03 Jun 2017 04:08:15: #2 predicted fragment length is 52 bps INFO @ Sat, 03 Jun 2017 04:08:15: #2 alternative fragment length(s) may be 52,131,530 bps INFO @ Sat, 03 Jun 2017 04:08:15: #2.2 Generate R script for model : SRX1388747.05_model.r WARNING @ Sat, 03 Jun 2017 04:08:15: #2 Since the d (52) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Jun 2017 04:08:15: #2 You may need to consider one of the other alternative d(s): 52,131,530 WARNING @ Sat, 03 Jun 2017 04:08:15: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Jun 2017 04:08:15: #3 Call peaks... INFO @ Sat, 03 Jun 2017 04:08:15: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Jun 2017 04:08:16: start X-correlation... INFO @ Sat, 03 Jun 2017 04:08:16: end of X-cor INFO @ Sat, 03 Jun 2017 04:08:16: #2 finished! INFO @ Sat, 03 Jun 2017 04:08:16: #2 predicted fragment length is 52 bps INFO @ Sat, 03 Jun 2017 04:08:16: #2 alternative fragment length(s) may be 52,131,530 bps INFO @ Sat, 03 Jun 2017 04:08:16: #2.2 Generate R script for model : SRX1388747.20_model.r WARNING @ Sat, 03 Jun 2017 04:08:16: #2 Since the d (52) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Jun 2017 04:08:16: #2 You may need to consider one of the other alternative d(s): 52,131,530 WARNING @ Sat, 03 Jun 2017 04:08:16: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Jun 2017 04:08:16: #3 Call peaks... INFO @ Sat, 03 Jun 2017 04:08:16: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Jun 2017 04:08:20: #3 Call peaks for each chromosome... INFO @ Sat, 03 Jun 2017 04:08:21: #3 Call peaks for each chromosome... INFO @ Sat, 03 Jun 2017 04:08:21: #3 Call peaks for each chromosome... INFO @ Sat, 03 Jun 2017 04:08:24: #4 Write output xls file... SRX1388747.05_peaks.xls INFO @ Sat, 03 Jun 2017 04:08:24: #4 Write peak in narrowPeak format file... SRX1388747.05_peaks.narrowPeak INFO @ Sat, 03 Jun 2017 04:08:24: #4 Write summits bed file... SRX1388747.05_summits.bed INFO @ Sat, 03 Jun 2017 04:08:24: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (205 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sat, 03 Jun 2017 04:08:25: #4 Write output xls file... SRX1388747.10_peaks.xls INFO @ Sat, 03 Jun 2017 04:08:25: #4 Write peak in narrowPeak format file... SRX1388747.10_peaks.narrowPeak INFO @ Sat, 03 Jun 2017 04:08:25: #4 Write summits bed file... SRX1388747.10_summits.bed INFO @ Sat, 03 Jun 2017 04:08:25: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (102 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Sat, 03 Jun 2017 04:08:25: #4 Write output xls file... SRX1388747.20_peaks.xls INFO @ Sat, 03 Jun 2017 04:08:25: #4 Write peak in narrowPeak format file... SRX1388747.20_peaks.narrowPeak INFO @ Sat, 03 Jun 2017 04:08:25: #4 Write summits bed file... SRX1388747.20_summits.bed INFO @ Sat, 03 Jun 2017 04:08:25: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (43 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。