Job ID = 16436116 SRX = SRX13110391 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2022-08-02T01:54:01 prefetch.2.10.7: 1) Downloading 'SRR16918061'... 2022-08-02T01:54:01 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T01:55:24 prefetch.2.10.7: HTTPS download succeed 2022-08-02T01:55:24 prefetch.2.10.7: 1) 'SRR16918061' was downloaded successfully 2022-08-02T01:55:24 prefetch.2.10.7: 'SRR16918061' has 0 unresolved dependencies Read 37731320 spots for SRR16918061/SRR16918061.sra Written 37731320 spots for SRR16918061/SRR16918061.sra fastq に変換しました。 bowtie でマッピング中... Your job 16436260 ("srTce11") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:00 37731320 reads; of these: 37731320 (100.00%) were unpaired; of these: 2529795 (6.70%) aligned 0 times 29714286 (78.75%) aligned exactly 1 time 5487239 (14.54%) aligned >1 times 93.30% overall alignment rate Time searching: 00:13:01 Overall time: 00:13:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5476608 / 35201525 = 0.1556 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 11:21:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 11:21:42: #1 read tag files... INFO @ Tue, 02 Aug 2022 11:21:42: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 11:21:50: 1000000 INFO @ Tue, 02 Aug 2022 11:21:59: 2000000 INFO @ Tue, 02 Aug 2022 11:22:07: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 11:22:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 11:22:11: #1 read tag files... INFO @ Tue, 02 Aug 2022 11:22:11: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 11:22:16: 4000000 INFO @ Tue, 02 Aug 2022 11:22:20: 1000000 INFO @ Tue, 02 Aug 2022 11:22:25: 5000000 INFO @ Tue, 02 Aug 2022 11:22:30: 2000000 INFO @ Tue, 02 Aug 2022 11:22:34: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 11:22:39: 3000000 INFO @ Tue, 02 Aug 2022 11:22:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 11:22:41: #1 read tag files... INFO @ Tue, 02 Aug 2022 11:22:41: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 11:22:43: 7000000 INFO @ Tue, 02 Aug 2022 11:22:49: 4000000 INFO @ Tue, 02 Aug 2022 11:22:50: 1000000 INFO @ Tue, 02 Aug 2022 11:22:51: 8000000 INFO @ Tue, 02 Aug 2022 11:22:58: 5000000 INFO @ Tue, 02 Aug 2022 11:23:00: 2000000 INFO @ Tue, 02 Aug 2022 11:23:00: 9000000 INFO @ Tue, 02 Aug 2022 11:23:08: 6000000 INFO @ Tue, 02 Aug 2022 11:23:09: 10000000 INFO @ Tue, 02 Aug 2022 11:23:09: 3000000 INFO @ Tue, 02 Aug 2022 11:23:17: 7000000 INFO @ Tue, 02 Aug 2022 11:23:17: 11000000 INFO @ Tue, 02 Aug 2022 11:23:19: 4000000 INFO @ Tue, 02 Aug 2022 11:23:26: 12000000 INFO @ Tue, 02 Aug 2022 11:23:26: 8000000 INFO @ Tue, 02 Aug 2022 11:23:28: 5000000 INFO @ Tue, 02 Aug 2022 11:23:35: 13000000 INFO @ Tue, 02 Aug 2022 11:23:36: 9000000 INFO @ Tue, 02 Aug 2022 11:23:38: 6000000 INFO @ Tue, 02 Aug 2022 11:23:43: 14000000 INFO @ Tue, 02 Aug 2022 11:23:46: 10000000 INFO @ Tue, 02 Aug 2022 11:23:47: 7000000 INFO @ Tue, 02 Aug 2022 11:23:52: 15000000 INFO @ Tue, 02 Aug 2022 11:23:55: 11000000 INFO @ Tue, 02 Aug 2022 11:23:57: 8000000 INFO @ Tue, 02 Aug 2022 11:24:00: 16000000 INFO @ Tue, 02 Aug 2022 11:24:04: 12000000 INFO @ Tue, 02 Aug 2022 11:24:06: 9000000 INFO @ Tue, 02 Aug 2022 11:24:09: 17000000 INFO @ Tue, 02 Aug 2022 11:24:14: 13000000 INFO @ Tue, 02 Aug 2022 11:24:15: 10000000 INFO @ Tue, 02 Aug 2022 11:24:17: 18000000 INFO @ Tue, 02 Aug 2022 11:24:24: 14000000 INFO @ Tue, 02 Aug 2022 11:24:25: 11000000 INFO @ Tue, 02 Aug 2022 11:24:26: 19000000 INFO @ Tue, 02 Aug 2022 11:24:33: 15000000 INFO @ Tue, 02 Aug 2022 11:24:34: 12000000 INFO @ Tue, 02 Aug 2022 11:24:35: 20000000 INFO @ Tue, 02 Aug 2022 11:24:43: 16000000 INFO @ Tue, 02 Aug 2022 11:24:43: 21000000 INFO @ Tue, 02 Aug 2022 11:24:43: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 11:24:52: 22000000 INFO @ Tue, 02 Aug 2022 11:24:52: 17000000 INFO @ Tue, 02 Aug 2022 11:24:53: 14000000 INFO @ Tue, 02 Aug 2022 11:25:00: 23000000 INFO @ Tue, 02 Aug 2022 11:25:02: 18000000 INFO @ Tue, 02 Aug 2022 11:25:02: 15000000 INFO @ Tue, 02 Aug 2022 11:25:09: 24000000 INFO @ Tue, 02 Aug 2022 11:25:11: 19000000 INFO @ Tue, 02 Aug 2022 11:25:12: 16000000 INFO @ Tue, 02 Aug 2022 11:25:18: 25000000 INFO @ Tue, 02 Aug 2022 11:25:21: 20000000 INFO @ Tue, 02 Aug 2022 11:25:22: 17000000 INFO @ Tue, 02 Aug 2022 11:25:26: 26000000 INFO @ Tue, 02 Aug 2022 11:25:31: 21000000 INFO @ Tue, 02 Aug 2022 11:25:31: 18000000 INFO @ Tue, 02 Aug 2022 11:25:35: 27000000 INFO @ Tue, 02 Aug 2022 11:25:40: 22000000 INFO @ Tue, 02 Aug 2022 11:25:41: 19000000 INFO @ Tue, 02 Aug 2022 11:25:43: 28000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 11:25:49: 23000000 INFO @ Tue, 02 Aug 2022 11:25:51: 20000000 INFO @ Tue, 02 Aug 2022 11:25:52: 29000000 INFO @ Tue, 02 Aug 2022 11:25:58: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 11:25:58: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 11:25:58: #1 total tags in treatment: 29724917 INFO @ Tue, 02 Aug 2022 11:25:58: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 11:25:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 11:25:58: #1 tags after filtering in treatment: 29724917 INFO @ Tue, 02 Aug 2022 11:25:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 11:25:58: #1 finished! INFO @ Tue, 02 Aug 2022 11:25:58: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 11:25:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 11:25:59: 24000000 INFO @ Tue, 02 Aug 2022 11:26:00: #2 number of paired peaks: 77 WARNING @ Tue, 02 Aug 2022 11:26:00: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 11:26:00: Process for pairing-model is terminated! INFO @ Tue, 02 Aug 2022 11:26:00: 21000000 cut: /home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 11:26:09: 25000000 INFO @ Tue, 02 Aug 2022 11:26:10: 22000000 INFO @ Tue, 02 Aug 2022 11:26:18: 26000000 INFO @ Tue, 02 Aug 2022 11:26:20: 23000000 INFO @ Tue, 02 Aug 2022 11:26:28: 27000000 INFO @ Tue, 02 Aug 2022 11:26:29: 24000000 INFO @ Tue, 02 Aug 2022 11:26:37: 28000000 INFO @ Tue, 02 Aug 2022 11:26:39: 25000000 INFO @ Tue, 02 Aug 2022 11:26:47: 29000000 INFO @ Tue, 02 Aug 2022 11:26:48: 26000000 INFO @ Tue, 02 Aug 2022 11:26:53: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 11:26:53: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 11:26:53: #1 total tags in treatment: 29724917 INFO @ Tue, 02 Aug 2022 11:26:53: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 11:26:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 11:26:54: #1 tags after filtering in treatment: 29724917 INFO @ Tue, 02 Aug 2022 11:26:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 11:26:54: #1 finished! INFO @ Tue, 02 Aug 2022 11:26:54: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 11:26:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 11:26:56: #2 number of paired peaks: 77 WARNING @ Tue, 02 Aug 2022 11:26:56: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 11:26:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 11:26:57: 27000000 INFO @ Tue, 02 Aug 2022 11:27:06: 28000000 INFO @ Tue, 02 Aug 2022 11:27:15: 29000000 INFO @ Tue, 02 Aug 2022 11:27:21: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 11:27:21: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 11:27:21: #1 total tags in treatment: 29724917 INFO @ Tue, 02 Aug 2022 11:27:21: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 11:27:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 11:27:21: #1 tags after filtering in treatment: 29724917 INFO @ Tue, 02 Aug 2022 11:27:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 11:27:21: #1 finished! INFO @ Tue, 02 Aug 2022 11:27:21: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 11:27:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 11:27:23: #2 number of paired peaks: 77 WARNING @ Tue, 02 Aug 2022 11:27:23: Too few paired peaks (77) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 11:27:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX13110391/SRX13110391.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling