Job ID = 16432499 SRX = SRX12572570 Genome = ce10 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-08-02T00:32:23 prefetch.2.10.7: 1) Downloading 'SRR16293605'... 2022-08-02T00:32:24 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T00:37:01 prefetch.2.10.7: HTTPS download succeed 2022-08-02T00:37:01 prefetch.2.10.7: 1) 'SRR16293605' was downloaded successfully 2022-08-02T00:37:01 prefetch.2.10.7: 'SRR16293605' has 0 unresolved dependencies Read 43663273 spots for SRR16293605/SRR16293605.sra Written 43663273 spots for SRR16293605/SRR16293605.sra fastq に変換しました。 bowtie でマッピング中... Your job 16436572 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:52:22 43663273 reads; of these: 43663273 (100.00%) were paired; of these: 4514227 (10.34%) aligned concordantly 0 times 33313543 (76.30%) aligned concordantly exactly 1 time 5835503 (13.36%) aligned concordantly >1 times ---- 4514227 pairs aligned concordantly 0 times; of these: 1792287 (39.70%) aligned discordantly 1 time ---- 2721940 pairs aligned 0 times concordantly or discordantly; of these: 5443880 mates make up the pairs; of these: 4236930 (77.83%) aligned 0 times 650218 (11.94%) aligned exactly 1 time 556732 (10.23%) aligned >1 times 95.15% overall alignment rate Time searching: 01:52:22 Overall time: 01:52:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 64 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 6830958 / 40804069 = 0.1674 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:14:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:14:33: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:14:33: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:14:45: 1000000 INFO @ Tue, 02 Aug 2022 12:14:57: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:15:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:15:02: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:15:02: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:15:08: 3000000 INFO @ Tue, 02 Aug 2022 12:15:14: 1000000 INFO @ Tue, 02 Aug 2022 12:15:19: 4000000 INFO @ Tue, 02 Aug 2022 12:15:26: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 12:15:31: 5000000 INFO @ Tue, 02 Aug 2022 12:15:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 12:15:32: #1 read tag files... INFO @ Tue, 02 Aug 2022 12:15:32: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 12:15:38: 3000000 INFO @ Tue, 02 Aug 2022 12:15:41: 1000000 INFO @ Tue, 02 Aug 2022 12:15:43: 6000000 INFO @ Tue, 02 Aug 2022 12:15:50: 2000000 INFO @ Tue, 02 Aug 2022 12:15:50: 4000000 INFO @ Tue, 02 Aug 2022 12:15:55: 7000000 INFO @ Tue, 02 Aug 2022 12:15:58: 3000000 INFO @ Tue, 02 Aug 2022 12:16:02: 5000000 INFO @ Tue, 02 Aug 2022 12:16:07: 4000000 INFO @ Tue, 02 Aug 2022 12:16:07: 8000000 INFO @ Tue, 02 Aug 2022 12:16:13: 6000000 INFO @ Tue, 02 Aug 2022 12:16:15: 5000000 INFO @ Tue, 02 Aug 2022 12:16:18: 9000000 INFO @ Tue, 02 Aug 2022 12:16:23: 6000000 INFO @ Tue, 02 Aug 2022 12:16:25: 7000000 INFO @ Tue, 02 Aug 2022 12:16:30: 10000000 INFO @ Tue, 02 Aug 2022 12:16:31: 7000000 INFO @ Tue, 02 Aug 2022 12:16:37: 8000000 INFO @ Tue, 02 Aug 2022 12:16:40: 8000000 INFO @ Tue, 02 Aug 2022 12:16:41: 11000000 INFO @ Tue, 02 Aug 2022 12:16:48: 9000000 INFO @ Tue, 02 Aug 2022 12:16:48: 9000000 INFO @ Tue, 02 Aug 2022 12:16:53: 12000000 INFO @ Tue, 02 Aug 2022 12:16:56: 10000000 INFO @ Tue, 02 Aug 2022 12:16:59: 10000000 INFO @ Tue, 02 Aug 2022 12:17:03: 13000000 INFO @ Tue, 02 Aug 2022 12:17:05: 11000000 INFO @ Tue, 02 Aug 2022 12:17:10: 11000000 INFO @ Tue, 02 Aug 2022 12:17:13: 12000000 INFO @ Tue, 02 Aug 2022 12:17:14: 14000000 INFO @ Tue, 02 Aug 2022 12:17:21: 12000000 INFO @ Tue, 02 Aug 2022 12:17:21: 13000000 INFO @ Tue, 02 Aug 2022 12:17:24: 15000000 INFO @ Tue, 02 Aug 2022 12:17:29: 14000000 INFO @ Tue, 02 Aug 2022 12:17:31: 13000000 INFO @ Tue, 02 Aug 2022 12:17:35: 16000000 INFO @ Tue, 02 Aug 2022 12:17:38: 15000000 INFO @ Tue, 02 Aug 2022 12:17:42: 14000000 INFO @ Tue, 02 Aug 2022 12:17:45: 17000000 INFO @ Tue, 02 Aug 2022 12:17:46: 16000000 INFO @ Tue, 02 Aug 2022 12:17:51: 15000000 INFO @ Tue, 02 Aug 2022 12:17:54: 18000000 INFO @ Tue, 02 Aug 2022 12:17:54: 17000000 INFO @ Tue, 02 Aug 2022 12:18:00: 16000000 INFO @ Tue, 02 Aug 2022 12:18:02: 18000000 INFO @ Tue, 02 Aug 2022 12:18:03: 19000000 INFO @ Tue, 02 Aug 2022 12:18:09: 17000000 INFO @ Tue, 02 Aug 2022 12:18:10: 19000000 INFO @ Tue, 02 Aug 2022 12:18:12: 20000000 INFO @ Tue, 02 Aug 2022 12:18:19: 20000000 INFO @ Tue, 02 Aug 2022 12:18:19: 18000000 INFO @ Tue, 02 Aug 2022 12:18:21: 21000000 INFO @ Tue, 02 Aug 2022 12:18:27: 21000000 INFO @ Tue, 02 Aug 2022 12:18:28: 19000000 INFO @ Tue, 02 Aug 2022 12:18:31: 22000000 INFO @ Tue, 02 Aug 2022 12:18:35: 22000000 INFO @ Tue, 02 Aug 2022 12:18:37: 20000000 INFO @ Tue, 02 Aug 2022 12:18:40: 23000000 INFO @ Tue, 02 Aug 2022 12:18:44: 23000000 INFO @ Tue, 02 Aug 2022 12:18:46: 21000000 INFO @ Tue, 02 Aug 2022 12:18:49: 24000000 INFO @ Tue, 02 Aug 2022 12:18:52: 24000000 INFO @ Tue, 02 Aug 2022 12:18:55: 22000000 INFO @ Tue, 02 Aug 2022 12:18:58: 25000000 INFO @ Tue, 02 Aug 2022 12:19:01: 25000000 INFO @ Tue, 02 Aug 2022 12:19:05: 23000000 INFO @ Tue, 02 Aug 2022 12:19:07: 26000000 INFO @ Tue, 02 Aug 2022 12:19:09: 26000000 INFO @ Tue, 02 Aug 2022 12:19:14: 24000000 INFO @ Tue, 02 Aug 2022 12:19:16: 27000000 INFO @ Tue, 02 Aug 2022 12:19:17: 27000000 INFO @ Tue, 02 Aug 2022 12:19:24: 25000000 INFO @ Tue, 02 Aug 2022 12:19:26: 28000000 INFO @ Tue, 02 Aug 2022 12:19:26: 28000000 INFO @ Tue, 02 Aug 2022 12:19:33: 26000000 INFO @ Tue, 02 Aug 2022 12:19:34: 29000000 INFO @ Tue, 02 Aug 2022 12:19:36: 29000000 INFO @ Tue, 02 Aug 2022 12:19:43: 30000000 INFO @ Tue, 02 Aug 2022 12:19:43: 27000000 INFO @ Tue, 02 Aug 2022 12:19:45: 30000000 INFO @ Tue, 02 Aug 2022 12:19:51: 31000000 INFO @ Tue, 02 Aug 2022 12:19:52: 28000000 INFO @ Tue, 02 Aug 2022 12:19:55: 31000000 INFO @ Tue, 02 Aug 2022 12:19:59: 32000000 INFO @ Tue, 02 Aug 2022 12:20:02: 29000000 INFO @ Tue, 02 Aug 2022 12:20:05: 32000000 INFO @ Tue, 02 Aug 2022 12:20:08: 33000000 INFO @ Tue, 02 Aug 2022 12:20:11: 30000000 INFO @ Tue, 02 Aug 2022 12:20:14: 33000000 INFO @ Tue, 02 Aug 2022 12:20:16: 34000000 INFO @ Tue, 02 Aug 2022 12:20:20: 31000000 INFO @ Tue, 02 Aug 2022 12:20:24: 34000000 INFO @ Tue, 02 Aug 2022 12:20:25: 35000000 INFO @ Tue, 02 Aug 2022 12:20:30: 32000000 INFO @ Tue, 02 Aug 2022 12:20:33: 36000000 INFO @ Tue, 02 Aug 2022 12:20:33: 35000000 INFO @ Tue, 02 Aug 2022 12:20:39: 33000000 INFO @ Tue, 02 Aug 2022 12:20:41: 37000000 INFO @ Tue, 02 Aug 2022 12:20:43: 36000000 INFO @ Tue, 02 Aug 2022 12:20:49: 34000000 INFO @ Tue, 02 Aug 2022 12:20:50: 38000000 INFO @ Tue, 02 Aug 2022 12:20:53: 37000000 INFO @ Tue, 02 Aug 2022 12:20:58: 39000000 INFO @ Tue, 02 Aug 2022 12:20:58: 35000000 INFO @ Tue, 02 Aug 2022 12:21:02: 38000000 INFO @ Tue, 02 Aug 2022 12:21:06: 40000000 INFO @ Tue, 02 Aug 2022 12:21:08: 36000000 INFO @ Tue, 02 Aug 2022 12:21:12: 39000000 INFO @ Tue, 02 Aug 2022 12:21:15: 41000000 INFO @ Tue, 02 Aug 2022 12:21:18: 37000000 INFO @ Tue, 02 Aug 2022 12:21:22: 40000000 INFO @ Tue, 02 Aug 2022 12:21:24: 42000000 INFO @ Tue, 02 Aug 2022 12:21:27: 38000000 INFO @ Tue, 02 Aug 2022 12:21:31: 41000000 INFO @ Tue, 02 Aug 2022 12:21:32: 43000000 INFO @ Tue, 02 Aug 2022 12:21:37: 39000000 INFO @ Tue, 02 Aug 2022 12:21:40: 44000000 INFO @ Tue, 02 Aug 2022 12:21:41: 42000000 INFO @ Tue, 02 Aug 2022 12:21:46: 40000000 INFO @ Tue, 02 Aug 2022 12:21:48: 45000000 INFO @ Tue, 02 Aug 2022 12:21:50: 43000000 INFO @ Tue, 02 Aug 2022 12:21:56: 41000000 INFO @ Tue, 02 Aug 2022 12:21:56: 46000000 INFO @ Tue, 02 Aug 2022 12:22:00: 44000000 INFO @ Tue, 02 Aug 2022 12:22:04: 47000000 INFO @ Tue, 02 Aug 2022 12:22:05: 42000000 INFO @ Tue, 02 Aug 2022 12:22:10: 45000000 INFO @ Tue, 02 Aug 2022 12:22:12: 48000000 INFO @ Tue, 02 Aug 2022 12:22:15: 43000000 INFO @ Tue, 02 Aug 2022 12:22:19: 46000000 INFO @ Tue, 02 Aug 2022 12:22:20: 49000000 INFO @ Tue, 02 Aug 2022 12:22:24: 44000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 12:22:28: 50000000 INFO @ Tue, 02 Aug 2022 12:22:29: 47000000 INFO @ Tue, 02 Aug 2022 12:22:34: 45000000 INFO @ Tue, 02 Aug 2022 12:22:36: 51000000 INFO @ Tue, 02 Aug 2022 12:22:38: 48000000 INFO @ Tue, 02 Aug 2022 12:22:43: 46000000 INFO @ Tue, 02 Aug 2022 12:22:44: 52000000 INFO @ Tue, 02 Aug 2022 12:22:48: 49000000 INFO @ Tue, 02 Aug 2022 12:22:52: 53000000 INFO @ Tue, 02 Aug 2022 12:22:53: 47000000 INFO @ Tue, 02 Aug 2022 12:22:57: 50000000 INFO @ Tue, 02 Aug 2022 12:23:00: 54000000 INFO @ Tue, 02 Aug 2022 12:23:02: 48000000 INFO @ Tue, 02 Aug 2022 12:23:07: 51000000 INFO @ Tue, 02 Aug 2022 12:23:09: 55000000 INFO @ Tue, 02 Aug 2022 12:23:11: 49000000 INFO @ Tue, 02 Aug 2022 12:23:16: 52000000 INFO @ Tue, 02 Aug 2022 12:23:17: 56000000 INFO @ Tue, 02 Aug 2022 12:23:21: 50000000 INFO @ Tue, 02 Aug 2022 12:23:25: 57000000 INFO @ Tue, 02 Aug 2022 12:23:26: 53000000 INFO @ Tue, 02 Aug 2022 12:23:31: 51000000 INFO @ Tue, 02 Aug 2022 12:23:33: 58000000 INFO @ Tue, 02 Aug 2022 12:23:35: 54000000 INFO @ Tue, 02 Aug 2022 12:23:40: 52000000 INFO @ Tue, 02 Aug 2022 12:23:41: 59000000 INFO @ Tue, 02 Aug 2022 12:23:45: 55000000 INFO @ Tue, 02 Aug 2022 12:23:49: 60000000 INFO @ Tue, 02 Aug 2022 12:23:50: 53000000 INFO @ Tue, 02 Aug 2022 12:23:54: 56000000 INFO @ Tue, 02 Aug 2022 12:23:57: 61000000 INFO @ Tue, 02 Aug 2022 12:23:59: 54000000 INFO @ Tue, 02 Aug 2022 12:24:04: 57000000 INFO @ Tue, 02 Aug 2022 12:24:05: 62000000 INFO @ Tue, 02 Aug 2022 12:24:09: 55000000 INFO @ Tue, 02 Aug 2022 12:24:13: 58000000 INFO @ Tue, 02 Aug 2022 12:24:14: 63000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 12:24:18: 56000000 INFO @ Tue, 02 Aug 2022 12:24:22: 64000000 INFO @ Tue, 02 Aug 2022 12:24:23: 59000000 INFO @ Tue, 02 Aug 2022 12:24:28: 57000000 INFO @ Tue, 02 Aug 2022 12:24:30: 65000000 INFO @ Tue, 02 Aug 2022 12:24:32: 60000000 INFO @ Tue, 02 Aug 2022 12:24:38: 58000000 INFO @ Tue, 02 Aug 2022 12:24:38: 66000000 INFO @ Tue, 02 Aug 2022 12:24:42: 61000000 INFO @ Tue, 02 Aug 2022 12:24:46: 67000000 INFO @ Tue, 02 Aug 2022 12:24:47: 59000000 INFO @ Tue, 02 Aug 2022 12:24:52: 62000000 INFO @ Tue, 02 Aug 2022 12:24:55: 68000000 INFO @ Tue, 02 Aug 2022 12:24:57: 60000000 INFO @ Tue, 02 Aug 2022 12:25:01: 63000000 INFO @ Tue, 02 Aug 2022 12:25:03: 69000000 INFO @ Tue, 02 Aug 2022 12:25:06: 61000000 INFO @ Tue, 02 Aug 2022 12:25:07: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 12:25:07: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 12:25:07: #1 total tags in treatment: 32507742 INFO @ Tue, 02 Aug 2022 12:25:07: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:25:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:25:07: #1 tags after filtering in treatment: 27988123 INFO @ Tue, 02 Aug 2022 12:25:07: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 02 Aug 2022 12:25:07: #1 finished! INFO @ Tue, 02 Aug 2022 12:25:07: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:25:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:25:09: #2 number of paired peaks: 135 WARNING @ Tue, 02 Aug 2022 12:25:09: Fewer paired peaks (135) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 135 pairs to build model! INFO @ Tue, 02 Aug 2022 12:25:09: start model_add_line... INFO @ Tue, 02 Aug 2022 12:25:09: start X-correlation... INFO @ Tue, 02 Aug 2022 12:25:10: end of X-cor INFO @ Tue, 02 Aug 2022 12:25:10: #2 finished! INFO @ Tue, 02 Aug 2022 12:25:10: #2 predicted fragment length is 166 bps INFO @ Tue, 02 Aug 2022 12:25:10: #2 alternative fragment length(s) may be 3,166 bps INFO @ Tue, 02 Aug 2022 12:25:10: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.20_model.r WARNING @ Tue, 02 Aug 2022 12:25:10: #2 Since the d (166) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 12:25:10: #2 You may need to consider one of the other alternative d(s): 3,166 WARNING @ Tue, 02 Aug 2022 12:25:10: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 12:25:10: #3 Call peaks... INFO @ Tue, 02 Aug 2022 12:25:10: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 12:25:11: 64000000 INFO @ Tue, 02 Aug 2022 12:25:16: 62000000 INFO @ Tue, 02 Aug 2022 12:25:20: 65000000 INFO @ Tue, 02 Aug 2022 12:25:25: 63000000 INFO @ Tue, 02 Aug 2022 12:25:29: 66000000 INFO @ Tue, 02 Aug 2022 12:25:34: 64000000 INFO @ Tue, 02 Aug 2022 12:25:38: 67000000 INFO @ Tue, 02 Aug 2022 12:25:43: 65000000 INFO @ Tue, 02 Aug 2022 12:25:47: 68000000 INFO @ Tue, 02 Aug 2022 12:25:52: 66000000 INFO @ Tue, 02 Aug 2022 12:25:56: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 12:25:57: 69000000 INFO @ Tue, 02 Aug 2022 12:26:01: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 12:26:01: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 12:26:01: #1 total tags in treatment: 32507742 INFO @ Tue, 02 Aug 2022 12:26:01: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:26:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:26:01: 67000000 INFO @ Tue, 02 Aug 2022 12:26:01: #1 tags after filtering in treatment: 27988123 INFO @ Tue, 02 Aug 2022 12:26:01: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 02 Aug 2022 12:26:01: #1 finished! INFO @ Tue, 02 Aug 2022 12:26:01: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:26:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:26:03: #2 number of paired peaks: 135 WARNING @ Tue, 02 Aug 2022 12:26:03: Fewer paired peaks (135) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 135 pairs to build model! INFO @ Tue, 02 Aug 2022 12:26:03: start model_add_line... INFO @ Tue, 02 Aug 2022 12:26:03: start X-correlation... INFO @ Tue, 02 Aug 2022 12:26:03: end of X-cor INFO @ Tue, 02 Aug 2022 12:26:03: #2 finished! INFO @ Tue, 02 Aug 2022 12:26:03: #2 predicted fragment length is 166 bps INFO @ Tue, 02 Aug 2022 12:26:03: #2 alternative fragment length(s) may be 3,166 bps INFO @ Tue, 02 Aug 2022 12:26:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.05_model.r WARNING @ Tue, 02 Aug 2022 12:26:03: #2 Since the d (166) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 12:26:03: #2 You may need to consider one of the other alternative d(s): 3,166 WARNING @ Tue, 02 Aug 2022 12:26:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 12:26:03: #3 Call peaks... INFO @ Tue, 02 Aug 2022 12:26:03: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 12:26:09: 68000000 INFO @ Tue, 02 Aug 2022 12:26:15: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.20_peaks.xls INFO @ Tue, 02 Aug 2022 12:26:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 12:26:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.20_summits.bed INFO @ Tue, 02 Aug 2022 12:26:16: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (689 records, 4 fields): 142 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 12:26:17: 69000000 INFO @ Tue, 02 Aug 2022 12:26:21: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 12:26:21: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 12:26:21: #1 total tags in treatment: 32507742 INFO @ Tue, 02 Aug 2022 12:26:21: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 12:26:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 12:26:22: #1 tags after filtering in treatment: 27988123 INFO @ Tue, 02 Aug 2022 12:26:22: #1 Redundant rate of treatment: 0.14 INFO @ Tue, 02 Aug 2022 12:26:22: #1 finished! INFO @ Tue, 02 Aug 2022 12:26:22: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 12:26:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 12:26:24: #2 number of paired peaks: 135 WARNING @ Tue, 02 Aug 2022 12:26:24: Fewer paired peaks (135) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 135 pairs to build model! INFO @ Tue, 02 Aug 2022 12:26:24: start model_add_line... INFO @ Tue, 02 Aug 2022 12:26:24: start X-correlation... INFO @ Tue, 02 Aug 2022 12:26:24: end of X-cor INFO @ Tue, 02 Aug 2022 12:26:24: #2 finished! INFO @ Tue, 02 Aug 2022 12:26:24: #2 predicted fragment length is 166 bps INFO @ Tue, 02 Aug 2022 12:26:24: #2 alternative fragment length(s) may be 3,166 bps INFO @ Tue, 02 Aug 2022 12:26:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.10_model.r WARNING @ Tue, 02 Aug 2022 12:26:24: #2 Since the d (166) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 12:26:24: #2 You may need to consider one of the other alternative d(s): 3,166 WARNING @ Tue, 02 Aug 2022 12:26:24: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 12:26:24: #3 Call peaks... INFO @ Tue, 02 Aug 2022 12:26:24: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 12:26:50: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 12:27:10: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.05_peaks.xls INFO @ Tue, 02 Aug 2022 12:27:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 12:27:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.05_summits.bed INFO @ Tue, 02 Aug 2022 12:27:10: Done! INFO @ Tue, 02 Aug 2022 12:27:10: #3 Call peaks for each chromosome... pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (2983 records, 4 fields): 21 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 12:27:30: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.10_peaks.xls INFO @ Tue, 02 Aug 2022 12:27:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 12:27:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX12572570/SRX12572570.10_summits.bed INFO @ Tue, 02 Aug 2022 12:27:31: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1815 records, 4 fields): 178 millis CompletedMACS2peakCalling