Job ID = 16434827 SRX = SRX11633405 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 27402976 spots for SRR15329114/SRR15329114.sra Written 27402976 spots for SRR15329114/SRR15329114.sra fastq に変換しました。 bowtie でマッピング中... Your job 16434895 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:10:16 27402976 reads; of these: 27402976 (100.00%) were unpaired; of these: 1535451 (5.60%) aligned 0 times 22065065 (80.52%) aligned exactly 1 time 3802460 (13.88%) aligned >1 times 94.40% overall alignment rate Time searching: 00:10:17 Overall time: 00:10:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2771368 / 25867525 = 0.1071 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 10:22:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 10:22:12: #1 read tag files... INFO @ Tue, 02 Aug 2022 10:22:12: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 10:22:20: 1000000 INFO @ Tue, 02 Aug 2022 10:22:27: 2000000 INFO @ Tue, 02 Aug 2022 10:22:35: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 10:22:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 10:22:41: #1 read tag files... INFO @ Tue, 02 Aug 2022 10:22:41: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 10:22:43: 4000000 INFO @ Tue, 02 Aug 2022 10:22:49: 1000000 INFO @ Tue, 02 Aug 2022 10:22:51: 5000000 INFO @ Tue, 02 Aug 2022 10:22:56: 2000000 INFO @ Tue, 02 Aug 2022 10:22:59: 6000000 INFO @ Tue, 02 Aug 2022 10:23:04: 3000000 INFO @ Tue, 02 Aug 2022 10:23:07: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 10:23:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 10:23:11: #1 read tag files... INFO @ Tue, 02 Aug 2022 10:23:11: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 10:23:13: 4000000 INFO @ Tue, 02 Aug 2022 10:23:16: 8000000 INFO @ Tue, 02 Aug 2022 10:23:19: 1000000 INFO @ Tue, 02 Aug 2022 10:23:22: 5000000 INFO @ Tue, 02 Aug 2022 10:23:24: 9000000 INFO @ Tue, 02 Aug 2022 10:23:28: 2000000 INFO @ Tue, 02 Aug 2022 10:23:30: 6000000 INFO @ Tue, 02 Aug 2022 10:23:33: 10000000 INFO @ Tue, 02 Aug 2022 10:23:37: 3000000 INFO @ Tue, 02 Aug 2022 10:23:38: 7000000 INFO @ Tue, 02 Aug 2022 10:23:41: 11000000 INFO @ Tue, 02 Aug 2022 10:23:46: 4000000 INFO @ Tue, 02 Aug 2022 10:23:47: 8000000 INFO @ Tue, 02 Aug 2022 10:23:50: 12000000 INFO @ Tue, 02 Aug 2022 10:23:55: 5000000 INFO @ Tue, 02 Aug 2022 10:23:56: 9000000 INFO @ Tue, 02 Aug 2022 10:23:58: 13000000 INFO @ Tue, 02 Aug 2022 10:24:04: 6000000 INFO @ Tue, 02 Aug 2022 10:24:04: 10000000 INFO @ Tue, 02 Aug 2022 10:24:07: 14000000 INFO @ Tue, 02 Aug 2022 10:24:12: 7000000 INFO @ Tue, 02 Aug 2022 10:24:13: 11000000 INFO @ Tue, 02 Aug 2022 10:24:16: 15000000 INFO @ Tue, 02 Aug 2022 10:24:21: 8000000 INFO @ Tue, 02 Aug 2022 10:24:21: 12000000 INFO @ Tue, 02 Aug 2022 10:24:24: 16000000 INFO @ Tue, 02 Aug 2022 10:24:29: 13000000 INFO @ Tue, 02 Aug 2022 10:24:29: 9000000 INFO @ Tue, 02 Aug 2022 10:24:33: 17000000 INFO @ Tue, 02 Aug 2022 10:24:38: 14000000 INFO @ Tue, 02 Aug 2022 10:24:38: 10000000 INFO @ Tue, 02 Aug 2022 10:24:41: 18000000 INFO @ Tue, 02 Aug 2022 10:24:46: 11000000 INFO @ Tue, 02 Aug 2022 10:24:46: 15000000 INFO @ Tue, 02 Aug 2022 10:24:49: 19000000 INFO @ Tue, 02 Aug 2022 10:24:54: 16000000 INFO @ Tue, 02 Aug 2022 10:24:54: 12000000 INFO @ Tue, 02 Aug 2022 10:24:57: 20000000 INFO @ Tue, 02 Aug 2022 10:25:02: 17000000 INFO @ Tue, 02 Aug 2022 10:25:03: 13000000 INFO @ Tue, 02 Aug 2022 10:25:05: 21000000 INFO @ Tue, 02 Aug 2022 10:25:10: 18000000 INFO @ Tue, 02 Aug 2022 10:25:11: 14000000 INFO @ Tue, 02 Aug 2022 10:25:14: 22000000 INFO @ Tue, 02 Aug 2022 10:25:18: 19000000 INFO @ Tue, 02 Aug 2022 10:25:19: 15000000 INFO @ Tue, 02 Aug 2022 10:25:22: 23000000 INFO @ Tue, 02 Aug 2022 10:25:23: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 10:25:23: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 10:25:23: #1 total tags in treatment: 23096157 INFO @ Tue, 02 Aug 2022 10:25:23: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 10:25:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 10:25:23: #1 tags after filtering in treatment: 23096157 INFO @ Tue, 02 Aug 2022 10:25:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 10:25:23: #1 finished! INFO @ Tue, 02 Aug 2022 10:25:23: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 10:25:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 10:25:25: #2 number of paired peaks: 90 WARNING @ Tue, 02 Aug 2022 10:25:25: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 10:25:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 10:25:26: 20000000 INFO @ Tue, 02 Aug 2022 10:25:27: 16000000 INFO @ Tue, 02 Aug 2022 10:25:33: 21000000 INFO @ Tue, 02 Aug 2022 10:25:35: 17000000 INFO @ Tue, 02 Aug 2022 10:25:41: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 10:25:42: 18000000 INFO @ Tue, 02 Aug 2022 10:25:49: 23000000 INFO @ Tue, 02 Aug 2022 10:25:50: 19000000 INFO @ Tue, 02 Aug 2022 10:25:50: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 10:25:50: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 10:25:50: #1 total tags in treatment: 23096157 INFO @ Tue, 02 Aug 2022 10:25:50: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 10:25:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 10:25:50: #1 tags after filtering in treatment: 23096157 INFO @ Tue, 02 Aug 2022 10:25:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 10:25:50: #1 finished! INFO @ Tue, 02 Aug 2022 10:25:50: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 10:25:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 10:25:52: #2 number of paired peaks: 90 WARNING @ Tue, 02 Aug 2022 10:25:52: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 10:25:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 10:25:58: 20000000 INFO @ Tue, 02 Aug 2022 10:26:06: 21000000 INFO @ Tue, 02 Aug 2022 10:26:13: 22000000 INFO @ Tue, 02 Aug 2022 10:26:21: 23000000 INFO @ Tue, 02 Aug 2022 10:26:22: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 10:26:22: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 10:26:22: #1 total tags in treatment: 23096157 INFO @ Tue, 02 Aug 2022 10:26:22: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 10:26:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 10:26:22: #1 tags after filtering in treatment: 23096157 INFO @ Tue, 02 Aug 2022 10:26:22: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 10:26:22: #1 finished! INFO @ Tue, 02 Aug 2022 10:26:22: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 10:26:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 10:26:24: #2 number of paired peaks: 90 WARNING @ Tue, 02 Aug 2022 10:26:24: Too few paired peaks (90) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 10:26:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX11633405/SRX11633405.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。