Job ID = 9157371 sra ファイルのダウンロード中... Completed: 637880K bytes transferred in 7 seconds (681642K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 25944757 spots for /home/okishinya/chipatlas/results/ce10/SRX1132918/SRR2144367.sra Written 25944757 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:58 25944757 reads; of these: 25944757 (100.00%) were unpaired; of these: 1888844 (7.28%) aligned 0 times 20017871 (77.16%) aligned exactly 1 time 4038042 (15.56%) aligned >1 times 92.72% overall alignment rate Time searching: 00:09:58 Overall time: 00:09:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10224554 / 24055913 = 0.4250 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 11:46:58: # Command line: callpeak -t SRX1132918.bam -f BAM -g ce -n SRX1132918.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1132918.10 # format = BAM # ChIP-seq file = ['SRX1132918.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:46:58: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:46:58: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:46:58: # Command line: callpeak -t SRX1132918.bam -f BAM -g ce -n SRX1132918.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1132918.05 # format = BAM # ChIP-seq file = ['SRX1132918.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:46:58: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:46:58: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:46:58: # Command line: callpeak -t SRX1132918.bam -f BAM -g ce -n SRX1132918.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1132918.20 # format = BAM # ChIP-seq file = ['SRX1132918.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:46:58: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:46:58: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:47:04: 1000000 INFO @ Tue, 27 Jun 2017 11:47:04: 1000000 INFO @ Tue, 27 Jun 2017 11:47:04: 1000000 INFO @ Tue, 27 Jun 2017 11:47:10: 2000000 INFO @ Tue, 27 Jun 2017 11:47:11: 2000000 INFO @ Tue, 27 Jun 2017 11:47:11: 2000000 INFO @ Tue, 27 Jun 2017 11:47:17: 3000000 INFO @ Tue, 27 Jun 2017 11:47:17: 3000000 INFO @ Tue, 27 Jun 2017 11:47:18: 3000000 INFO @ Tue, 27 Jun 2017 11:47:23: 4000000 INFO @ Tue, 27 Jun 2017 11:47:24: 4000000 INFO @ Tue, 27 Jun 2017 11:47:24: 4000000 INFO @ Tue, 27 Jun 2017 11:47:30: 5000000 INFO @ Tue, 27 Jun 2017 11:47:30: 5000000 INFO @ Tue, 27 Jun 2017 11:47:31: 5000000 INFO @ Tue, 27 Jun 2017 11:47:36: 6000000 INFO @ Tue, 27 Jun 2017 11:47:37: 6000000 INFO @ Tue, 27 Jun 2017 11:47:38: 6000000 INFO @ Tue, 27 Jun 2017 11:47:43: 7000000 INFO @ Tue, 27 Jun 2017 11:47:44: 7000000 INFO @ Tue, 27 Jun 2017 11:47:45: 7000000 INFO @ Tue, 27 Jun 2017 11:47:49: 8000000 INFO @ Tue, 27 Jun 2017 11:47:50: 8000000 INFO @ Tue, 27 Jun 2017 11:47:52: 8000000 INFO @ Tue, 27 Jun 2017 11:47:56: 9000000 INFO @ Tue, 27 Jun 2017 11:47:57: 9000000 INFO @ Tue, 27 Jun 2017 11:47:59: 9000000 INFO @ Tue, 27 Jun 2017 11:48:02: 10000000 INFO @ Tue, 27 Jun 2017 11:48:04: 10000000 INFO @ Tue, 27 Jun 2017 11:48:06: 10000000 INFO @ Tue, 27 Jun 2017 11:48:08: 11000000 INFO @ Tue, 27 Jun 2017 11:48:10: 11000000 INFO @ Tue, 27 Jun 2017 11:48:13: 11000000 INFO @ Tue, 27 Jun 2017 11:48:15: 12000000 INFO @ Tue, 27 Jun 2017 11:48:17: 12000000 INFO @ Tue, 27 Jun 2017 11:48:19: 12000000 INFO @ Tue, 27 Jun 2017 11:48:21: 13000000 INFO @ Tue, 27 Jun 2017 11:48:24: 13000000 INFO @ Tue, 27 Jun 2017 11:48:26: 13000000 INFO @ Tue, 27 Jun 2017 11:48:27: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 11:48:27: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 11:48:27: #1 total tags in treatment: 13831359 INFO @ Tue, 27 Jun 2017 11:48:27: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:48:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:48:27: #1 tags after filtering in treatment: 13831359 INFO @ Tue, 27 Jun 2017 11:48:27: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:48:27: #1 finished! INFO @ Tue, 27 Jun 2017 11:48:27: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:48:27: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:48:28: #2 number of paired peaks: 38 WARNING @ Tue, 27 Jun 2017 11:48:28: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:48:28: Process for pairing-model is terminated! cat: SRX1132918.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1132918.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132918.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132918.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 11:48:29: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 11:48:29: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 11:48:29: #1 total tags in treatment: 13831359 INFO @ Tue, 27 Jun 2017 11:48:29: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:48:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:48:30: #1 tags after filtering in treatment: 13831359 INFO @ Tue, 27 Jun 2017 11:48:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:48:30: #1 finished! INFO @ Tue, 27 Jun 2017 11:48:30: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:48:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:48:30: #2 number of paired peaks: 38 WARNING @ Tue, 27 Jun 2017 11:48:30: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:48:30: Process for pairing-model is terminated! cat: SRX1132918.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1132918.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132918.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132918.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 11:48:32: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 11:48:32: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 11:48:32: #1 total tags in treatment: 13831359 INFO @ Tue, 27 Jun 2017 11:48:32: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:48:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:48:32: #1 tags after filtering in treatment: 13831359 INFO @ Tue, 27 Jun 2017 11:48:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:48:32: #1 finished! INFO @ Tue, 27 Jun 2017 11:48:32: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:48:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:48:33: #2 number of paired peaks: 38 WARNING @ Tue, 27 Jun 2017 11:48:33: Too few paired peaks (38) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:48:33: Process for pairing-model is terminated! cat: SRX1132918.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1132918.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132918.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132918.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。