Job ID = 9157368 sra ファイルのダウンロード中... Completed: 754089K bytes transferred in 8 seconds (707825K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 22878101 spots for /home/okishinya/chipatlas/results/ce10/SRX1132915/SRR2144364.sra Written 22878101 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:29 22878101 reads; of these: 22878101 (100.00%) were unpaired; of these: 997519 (4.36%) aligned 0 times 18546083 (81.06%) aligned exactly 1 time 3334499 (14.58%) aligned >1 times 95.64% overall alignment rate Time searching: 00:08:29 Overall time: 00:08:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7994533 / 21880582 = 0.3654 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 11:44:15: # Command line: callpeak -t SRX1132915.bam -f BAM -g ce -n SRX1132915.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1132915.20 # format = BAM # ChIP-seq file = ['SRX1132915.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:44:15: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:44:15: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:44:15: # Command line: callpeak -t SRX1132915.bam -f BAM -g ce -n SRX1132915.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1132915.05 # format = BAM # ChIP-seq file = ['SRX1132915.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:44:15: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:44:15: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:44:15: # Command line: callpeak -t SRX1132915.bam -f BAM -g ce -n SRX1132915.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1132915.10 # format = BAM # ChIP-seq file = ['SRX1132915.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:44:15: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:44:15: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:44:23: 1000000 INFO @ Tue, 27 Jun 2017 11:44:23: 1000000 INFO @ Tue, 27 Jun 2017 11:44:23: 1000000 INFO @ Tue, 27 Jun 2017 11:44:30: 2000000 INFO @ Tue, 27 Jun 2017 11:44:30: 2000000 INFO @ Tue, 27 Jun 2017 11:44:30: 2000000 INFO @ Tue, 27 Jun 2017 11:44:37: 3000000 INFO @ Tue, 27 Jun 2017 11:44:38: 3000000 INFO @ Tue, 27 Jun 2017 11:44:38: 3000000 INFO @ Tue, 27 Jun 2017 11:44:45: 4000000 INFO @ Tue, 27 Jun 2017 11:44:45: 4000000 INFO @ Tue, 27 Jun 2017 11:44:46: 4000000 INFO @ Tue, 27 Jun 2017 11:44:52: 5000000 INFO @ Tue, 27 Jun 2017 11:44:53: 5000000 INFO @ Tue, 27 Jun 2017 11:44:54: 5000000 INFO @ Tue, 27 Jun 2017 11:44:59: 6000000 INFO @ Tue, 27 Jun 2017 11:45:01: 6000000 INFO @ Tue, 27 Jun 2017 11:45:02: 6000000 INFO @ Tue, 27 Jun 2017 11:45:07: 7000000 INFO @ Tue, 27 Jun 2017 11:45:09: 7000000 INFO @ Tue, 27 Jun 2017 11:45:10: 7000000 INFO @ Tue, 27 Jun 2017 11:45:14: 8000000 INFO @ Tue, 27 Jun 2017 11:45:17: 8000000 INFO @ Tue, 27 Jun 2017 11:45:18: 8000000 INFO @ Tue, 27 Jun 2017 11:45:21: 9000000 INFO @ Tue, 27 Jun 2017 11:45:24: 9000000 INFO @ Tue, 27 Jun 2017 11:45:26: 9000000 INFO @ Tue, 27 Jun 2017 11:45:29: 10000000 INFO @ Tue, 27 Jun 2017 11:45:32: 10000000 INFO @ Tue, 27 Jun 2017 11:45:33: 10000000 INFO @ Tue, 27 Jun 2017 11:45:36: 11000000 INFO @ Tue, 27 Jun 2017 11:45:39: 11000000 INFO @ Tue, 27 Jun 2017 11:45:41: 11000000 INFO @ Tue, 27 Jun 2017 11:45:43: 12000000 INFO @ Tue, 27 Jun 2017 11:45:47: 12000000 INFO @ Tue, 27 Jun 2017 11:45:49: 12000000 INFO @ Tue, 27 Jun 2017 11:45:50: 13000000 INFO @ Tue, 27 Jun 2017 11:45:54: 13000000 INFO @ Tue, 27 Jun 2017 11:45:56: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 11:45:56: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 11:45:56: #1 total tags in treatment: 13886049 INFO @ Tue, 27 Jun 2017 11:45:56: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:45:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:45:56: #1 tags after filtering in treatment: 13886049 INFO @ Tue, 27 Jun 2017 11:45:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:45:56: #1 finished! INFO @ Tue, 27 Jun 2017 11:45:56: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:45:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:45:56: 13000000 INFO @ Tue, 27 Jun 2017 11:45:57: #2 number of paired peaks: 30 WARNING @ Tue, 27 Jun 2017 11:45:57: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:45:57: Process for pairing-model is terminated! cat: SRX1132915.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1132915.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132915.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132915.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 11:46:01: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 11:46:01: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 11:46:01: #1 total tags in treatment: 13886049 INFO @ Tue, 27 Jun 2017 11:46:01: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:46:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:46:01: #1 tags after filtering in treatment: 13886049 INFO @ Tue, 27 Jun 2017 11:46:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:46:01: #1 finished! INFO @ Tue, 27 Jun 2017 11:46:01: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:46:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:46:02: #2 number of paired peaks: 30 WARNING @ Tue, 27 Jun 2017 11:46:02: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:46:02: Process for pairing-model is terminated! cat: SRX1132915.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1132915.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132915.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132915.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 11:46:03: #1 tag size is determined as 50 bps INFO @ Tue, 27 Jun 2017 11:46:03: #1 tag size = 50 INFO @ Tue, 27 Jun 2017 11:46:03: #1 total tags in treatment: 13886049 INFO @ Tue, 27 Jun 2017 11:46:03: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:46:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:46:03: #1 tags after filtering in treatment: 13886049 INFO @ Tue, 27 Jun 2017 11:46:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:46:03: #1 finished! INFO @ Tue, 27 Jun 2017 11:46:03: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:46:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:46:04: #2 number of paired peaks: 30 WARNING @ Tue, 27 Jun 2017 11:46:04: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:46:04: Process for pairing-model is terminated! cat: SRX1132915.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1132915.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132915.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1132915.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。