Job ID = 2589363 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 34,725,715 reads read : 34,725,715 reads written : 34,725,715 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:48 34725715 reads; of these: 34725715 (100.00%) were unpaired; of these: 1111493 (3.20%) aligned 0 times 28204750 (81.22%) aligned exactly 1 time 5409472 (15.58%) aligned >1 times 96.80% overall alignment rate Time searching: 00:09:48 Overall time: 00:09:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5424816 / 33614222 = 0.1614 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 18:09:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:09:08: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:09:08: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:09:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:09:09: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:09:09: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:09:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 18:09:10: #1 read tag files... INFO @ Mon, 12 Aug 2019 18:09:10: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 18:09:17: 1000000 INFO @ Mon, 12 Aug 2019 18:09:17: 1000000 INFO @ Mon, 12 Aug 2019 18:09:18: 1000000 INFO @ Mon, 12 Aug 2019 18:09:25: 2000000 INFO @ Mon, 12 Aug 2019 18:09:25: 2000000 INFO @ Mon, 12 Aug 2019 18:09:26: 2000000 INFO @ Mon, 12 Aug 2019 18:09:32: 3000000 INFO @ Mon, 12 Aug 2019 18:09:33: 3000000 INFO @ Mon, 12 Aug 2019 18:09:35: 3000000 INFO @ Mon, 12 Aug 2019 18:09:39: 4000000 INFO @ Mon, 12 Aug 2019 18:09:41: 4000000 INFO @ Mon, 12 Aug 2019 18:09:45: 4000000 INFO @ Mon, 12 Aug 2019 18:09:46: 5000000 INFO @ Mon, 12 Aug 2019 18:09:49: 5000000 INFO @ Mon, 12 Aug 2019 18:09:54: 6000000 INFO @ Mon, 12 Aug 2019 18:09:54: 5000000 INFO @ Mon, 12 Aug 2019 18:09:57: 6000000 INFO @ Mon, 12 Aug 2019 18:10:01: 7000000 INFO @ Mon, 12 Aug 2019 18:10:03: 6000000 INFO @ Mon, 12 Aug 2019 18:10:05: 7000000 INFO @ Mon, 12 Aug 2019 18:10:08: 8000000 INFO @ Mon, 12 Aug 2019 18:10:12: 7000000 INFO @ Mon, 12 Aug 2019 18:10:13: 8000000 INFO @ Mon, 12 Aug 2019 18:10:15: 9000000 INFO @ Mon, 12 Aug 2019 18:10:20: 9000000 INFO @ Mon, 12 Aug 2019 18:10:21: 8000000 INFO @ Mon, 12 Aug 2019 18:10:22: 10000000 INFO @ Mon, 12 Aug 2019 18:10:28: 10000000 INFO @ Mon, 12 Aug 2019 18:10:29: 11000000 INFO @ Mon, 12 Aug 2019 18:10:31: 9000000 INFO @ Mon, 12 Aug 2019 18:10:36: 11000000 INFO @ Mon, 12 Aug 2019 18:10:37: 12000000 INFO @ Mon, 12 Aug 2019 18:10:40: 10000000 INFO @ Mon, 12 Aug 2019 18:10:44: 13000000 INFO @ Mon, 12 Aug 2019 18:10:44: 12000000 INFO @ Mon, 12 Aug 2019 18:10:49: 11000000 INFO @ Mon, 12 Aug 2019 18:10:51: 14000000 INFO @ Mon, 12 Aug 2019 18:10:52: 13000000 INFO @ Mon, 12 Aug 2019 18:10:58: 15000000 INFO @ Mon, 12 Aug 2019 18:10:58: 12000000 INFO @ Mon, 12 Aug 2019 18:11:00: 14000000 INFO @ Mon, 12 Aug 2019 18:11:05: 16000000 INFO @ Mon, 12 Aug 2019 18:11:07: 13000000 INFO @ Mon, 12 Aug 2019 18:11:08: 15000000 INFO @ Mon, 12 Aug 2019 18:11:12: 17000000 INFO @ Mon, 12 Aug 2019 18:11:15: 16000000 INFO @ Mon, 12 Aug 2019 18:11:17: 14000000 INFO @ Mon, 12 Aug 2019 18:11:20: 18000000 INFO @ Mon, 12 Aug 2019 18:11:23: 17000000 INFO @ Mon, 12 Aug 2019 18:11:26: 15000000 INFO @ Mon, 12 Aug 2019 18:11:27: 19000000 INFO @ Mon, 12 Aug 2019 18:11:31: 18000000 INFO @ Mon, 12 Aug 2019 18:11:34: 20000000 INFO @ Mon, 12 Aug 2019 18:11:35: 16000000 INFO @ Mon, 12 Aug 2019 18:11:39: 19000000 INFO @ Mon, 12 Aug 2019 18:11:41: 21000000 INFO @ Mon, 12 Aug 2019 18:11:45: 17000000 INFO @ Mon, 12 Aug 2019 18:11:47: 20000000 INFO @ Mon, 12 Aug 2019 18:11:48: 22000000 INFO @ Mon, 12 Aug 2019 18:11:54: 18000000 INFO @ Mon, 12 Aug 2019 18:11:55: 21000000 INFO @ Mon, 12 Aug 2019 18:11:56: 23000000 INFO @ Mon, 12 Aug 2019 18:12:03: 24000000 INFO @ Mon, 12 Aug 2019 18:12:03: 22000000 INFO @ Mon, 12 Aug 2019 18:12:04: 19000000 INFO @ Mon, 12 Aug 2019 18:12:10: 25000000 INFO @ Mon, 12 Aug 2019 18:12:11: 23000000 INFO @ Mon, 12 Aug 2019 18:12:13: 20000000 INFO @ Mon, 12 Aug 2019 18:12:19: 24000000 INFO @ Mon, 12 Aug 2019 18:12:19: 26000000 INFO @ Mon, 12 Aug 2019 18:12:23: 21000000 INFO @ Mon, 12 Aug 2019 18:12:27: 25000000 INFO @ Mon, 12 Aug 2019 18:12:28: 27000000 INFO @ Mon, 12 Aug 2019 18:12:32: 22000000 INFO @ Mon, 12 Aug 2019 18:12:35: 26000000 INFO @ Mon, 12 Aug 2019 18:12:37: 28000000 INFO @ Mon, 12 Aug 2019 18:12:39: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 18:12:39: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 18:12:39: #1 total tags in treatment: 28189406 INFO @ Mon, 12 Aug 2019 18:12:39: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:12:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:12:40: #1 tags after filtering in treatment: 28189406 INFO @ Mon, 12 Aug 2019 18:12:40: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:12:40: #1 finished! INFO @ Mon, 12 Aug 2019 18:12:40: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:12:40: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:12:42: #2 number of paired peaks: 98 WARNING @ Mon, 12 Aug 2019 18:12:42: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 18:12:42: Process for pairing-model is terminated! INFO @ Mon, 12 Aug 2019 18:12:42: 23000000 cut: /home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:12:42: 27000000 INFO @ Mon, 12 Aug 2019 18:12:50: 28000000 INFO @ Mon, 12 Aug 2019 18:12:51: 24000000 INFO @ Mon, 12 Aug 2019 18:12:52: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 18:12:52: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 18:12:52: #1 total tags in treatment: 28189406 INFO @ Mon, 12 Aug 2019 18:12:52: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:12:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:12:52: #1 tags after filtering in treatment: 28189406 INFO @ Mon, 12 Aug 2019 18:12:52: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:12:52: #1 finished! INFO @ Mon, 12 Aug 2019 18:12:52: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:12:52: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:12:55: #2 number of paired peaks: 98 WARNING @ Mon, 12 Aug 2019 18:12:55: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 18:12:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 18:13:00: 25000000 INFO @ Mon, 12 Aug 2019 18:13:09: 26000000 INFO @ Mon, 12 Aug 2019 18:13:18: 27000000 INFO @ Mon, 12 Aug 2019 18:13:27: 28000000 INFO @ Mon, 12 Aug 2019 18:13:29: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 18:13:29: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 18:13:29: #1 total tags in treatment: 28189406 INFO @ Mon, 12 Aug 2019 18:13:29: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 18:13:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 18:13:30: #1 tags after filtering in treatment: 28189406 INFO @ Mon, 12 Aug 2019 18:13:30: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 18:13:30: #1 finished! INFO @ Mon, 12 Aug 2019 18:13:30: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 18:13:30: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 18:13:32: #2 number of paired peaks: 98 WARNING @ Mon, 12 Aug 2019 18:13:32: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 18:13:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX1078716/SRX1078716.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。