Job ID = 1291520 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 45,762,552 reads read : 45,762,552 reads written : 45,762,552 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:48 45762552 reads; of these: 45762552 (100.00%) were unpaired; of these: 10733239 (23.45%) aligned 0 times 28769309 (62.87%) aligned exactly 1 time 6260004 (13.68%) aligned >1 times 76.55% overall alignment rate Time searching: 00:07:48 Overall time: 00:07:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5498204 / 35029313 = 0.1570 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 16:21:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 16:21:43: #1 read tag files... INFO @ Sun, 02 Jun 2019 16:21:43: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 16:21:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 16:21:43: #1 read tag files... INFO @ Sun, 02 Jun 2019 16:21:43: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 16:21:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 16:21:43: #1 read tag files... INFO @ Sun, 02 Jun 2019 16:21:43: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 16:21:51: 1000000 INFO @ Sun, 02 Jun 2019 16:21:53: 1000000 INFO @ Sun, 02 Jun 2019 16:21:54: 1000000 INFO @ Sun, 02 Jun 2019 16:22:00: 2000000 INFO @ Sun, 02 Jun 2019 16:22:04: 2000000 INFO @ Sun, 02 Jun 2019 16:22:05: 2000000 INFO @ Sun, 02 Jun 2019 16:22:08: 3000000 INFO @ Sun, 02 Jun 2019 16:22:14: 3000000 INFO @ Sun, 02 Jun 2019 16:22:16: 3000000 INFO @ Sun, 02 Jun 2019 16:22:17: 4000000 INFO @ Sun, 02 Jun 2019 16:22:24: 4000000 INFO @ Sun, 02 Jun 2019 16:22:25: 5000000 INFO @ Sun, 02 Jun 2019 16:22:27: 4000000 INFO @ Sun, 02 Jun 2019 16:22:34: 6000000 INFO @ Sun, 02 Jun 2019 16:22:35: 5000000 INFO @ Sun, 02 Jun 2019 16:22:38: 5000000 INFO @ Sun, 02 Jun 2019 16:22:42: 7000000 INFO @ Sun, 02 Jun 2019 16:22:45: 6000000 INFO @ Sun, 02 Jun 2019 16:22:48: 6000000 INFO @ Sun, 02 Jun 2019 16:22:50: 8000000 INFO @ Sun, 02 Jun 2019 16:22:55: 7000000 INFO @ Sun, 02 Jun 2019 16:22:59: 9000000 INFO @ Sun, 02 Jun 2019 16:22:59: 7000000 INFO @ Sun, 02 Jun 2019 16:23:05: 8000000 INFO @ Sun, 02 Jun 2019 16:23:07: 10000000 INFO @ Sun, 02 Jun 2019 16:23:09: 8000000 INFO @ Sun, 02 Jun 2019 16:23:15: 9000000 INFO @ Sun, 02 Jun 2019 16:23:15: 11000000 INFO @ Sun, 02 Jun 2019 16:23:20: 9000000 INFO @ Sun, 02 Jun 2019 16:23:24: 12000000 INFO @ Sun, 02 Jun 2019 16:23:25: 10000000 INFO @ Sun, 02 Jun 2019 16:23:31: 10000000 INFO @ Sun, 02 Jun 2019 16:23:32: 13000000 INFO @ Sun, 02 Jun 2019 16:23:35: 11000000 INFO @ Sun, 02 Jun 2019 16:23:41: 14000000 INFO @ Sun, 02 Jun 2019 16:23:42: 11000000 INFO @ Sun, 02 Jun 2019 16:23:45: 12000000 INFO @ Sun, 02 Jun 2019 16:23:49: 15000000 INFO @ Sun, 02 Jun 2019 16:23:52: 12000000 INFO @ Sun, 02 Jun 2019 16:23:55: 13000000 INFO @ Sun, 02 Jun 2019 16:23:58: 16000000 INFO @ Sun, 02 Jun 2019 16:24:03: 13000000 INFO @ Sun, 02 Jun 2019 16:24:06: 14000000 INFO @ Sun, 02 Jun 2019 16:24:06: 17000000 INFO @ Sun, 02 Jun 2019 16:24:14: 14000000 INFO @ Sun, 02 Jun 2019 16:24:15: 18000000 INFO @ Sun, 02 Jun 2019 16:24:16: 15000000 INFO @ Sun, 02 Jun 2019 16:24:23: 19000000 INFO @ Sun, 02 Jun 2019 16:24:25: 15000000 INFO @ Sun, 02 Jun 2019 16:24:26: 16000000 INFO @ Sun, 02 Jun 2019 16:24:32: 20000000 INFO @ Sun, 02 Jun 2019 16:24:35: 17000000 INFO @ Sun, 02 Jun 2019 16:24:35: 16000000 INFO @ Sun, 02 Jun 2019 16:24:40: 21000000 INFO @ Sun, 02 Jun 2019 16:24:45: 18000000 INFO @ Sun, 02 Jun 2019 16:24:46: 17000000 INFO @ Sun, 02 Jun 2019 16:24:48: 22000000 INFO @ Sun, 02 Jun 2019 16:24:55: 19000000 INFO @ Sun, 02 Jun 2019 16:24:56: 23000000 INFO @ Sun, 02 Jun 2019 16:24:57: 18000000 INFO @ Sun, 02 Jun 2019 16:25:04: 20000000 INFO @ Sun, 02 Jun 2019 16:25:08: 24000000 INFO @ Sun, 02 Jun 2019 16:25:08: 19000000 INFO @ Sun, 02 Jun 2019 16:25:14: 21000000 INFO @ Sun, 02 Jun 2019 16:25:17: 25000000 INFO @ Sun, 02 Jun 2019 16:25:18: 20000000 INFO @ Sun, 02 Jun 2019 16:25:23: 22000000 INFO @ Sun, 02 Jun 2019 16:25:25: 26000000 INFO @ Sun, 02 Jun 2019 16:25:29: 21000000 INFO @ Sun, 02 Jun 2019 16:25:33: 23000000 INFO @ Sun, 02 Jun 2019 16:25:34: 27000000 INFO @ Sun, 02 Jun 2019 16:25:39: 22000000 INFO @ Sun, 02 Jun 2019 16:25:42: 28000000 INFO @ Sun, 02 Jun 2019 16:25:43: 24000000 INFO @ Sun, 02 Jun 2019 16:25:50: 23000000 INFO @ Sun, 02 Jun 2019 16:25:50: 29000000 INFO @ Sun, 02 Jun 2019 16:25:53: 25000000 INFO @ Sun, 02 Jun 2019 16:25:55: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 16:25:55: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 16:25:55: #1 total tags in treatment: 29531109 INFO @ Sun, 02 Jun 2019 16:25:55: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 16:25:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 16:25:55: #1 tags after filtering in treatment: 29531109 INFO @ Sun, 02 Jun 2019 16:25:55: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 16:25:55: #1 finished! INFO @ Sun, 02 Jun 2019 16:25:55: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 16:25:55: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 16:25:58: #2 number of paired peaks: 63 WARNING @ Sun, 02 Jun 2019 16:25:58: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 16:25:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 16:26:00: 24000000 INFO @ Sun, 02 Jun 2019 16:26:02: 26000000 INFO @ Sun, 02 Jun 2019 16:26:11: 25000000 INFO @ Sun, 02 Jun 2019 16:26:12: 27000000 INFO @ Sun, 02 Jun 2019 16:26:22: 28000000 INFO @ Sun, 02 Jun 2019 16:26:22: 26000000 INFO @ Sun, 02 Jun 2019 16:26:32: 29000000 INFO @ Sun, 02 Jun 2019 16:26:32: 27000000 INFO @ Sun, 02 Jun 2019 16:26:37: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 16:26:37: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 16:26:37: #1 total tags in treatment: 29531109 INFO @ Sun, 02 Jun 2019 16:26:37: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 16:26:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 16:26:37: #1 tags after filtering in treatment: 29531109 INFO @ Sun, 02 Jun 2019 16:26:37: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 16:26:37: #1 finished! INFO @ Sun, 02 Jun 2019 16:26:37: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 16:26:37: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 16:26:40: #2 number of paired peaks: 63 WARNING @ Sun, 02 Jun 2019 16:26:40: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 16:26:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 16:26:41: 28000000 INFO @ Sun, 02 Jun 2019 16:26:51: 29000000 INFO @ Sun, 02 Jun 2019 16:26:57: #1 tag size is determined as 36 bps INFO @ Sun, 02 Jun 2019 16:26:57: #1 tag size = 36 INFO @ Sun, 02 Jun 2019 16:26:57: #1 total tags in treatment: 29531109 INFO @ Sun, 02 Jun 2019 16:26:57: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 16:26:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 16:26:57: #1 tags after filtering in treatment: 29531109 INFO @ Sun, 02 Jun 2019 16:26:57: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 16:26:57: #1 finished! INFO @ Sun, 02 Jun 2019 16:26:57: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 16:26:57: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 16:27:00: #2 number of paired peaks: 63 WARNING @ Sun, 02 Jun 2019 16:27:00: Too few paired peaks (63) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 16:27:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX105313/SRX105313.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。