Job ID = 16434845 SRX = SRX10435518 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 34975676 spots for SRR14060950/SRR14060950.sra Written 34975676 spots for SRR14060950/SRR14060950.sra fastq に変換しました。 bowtie でマッピング中... Your job 16434928 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:25 34975676 reads; of these: 34975676 (100.00%) were unpaired; of these: 711589 (2.03%) aligned 0 times 28473811 (81.41%) aligned exactly 1 time 5790276 (16.56%) aligned >1 times 97.97% overall alignment rate Time searching: 00:11:25 Overall time: 00:11:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5494112 / 34264087 = 0.1603 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 10:20:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 10:20:33: #1 read tag files... INFO @ Tue, 02 Aug 2022 10:20:33: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 10:20:39: 1000000 INFO @ Tue, 02 Aug 2022 10:20:45: 2000000 INFO @ Tue, 02 Aug 2022 10:20:51: 3000000 INFO @ Tue, 02 Aug 2022 10:20:58: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 10:21:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 10:21:02: #1 read tag files... INFO @ Tue, 02 Aug 2022 10:21:02: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 10:21:04: 5000000 INFO @ Tue, 02 Aug 2022 10:21:09: 1000000 INFO @ Tue, 02 Aug 2022 10:21:11: 6000000 INFO @ Tue, 02 Aug 2022 10:21:15: 2000000 INFO @ Tue, 02 Aug 2022 10:21:18: 7000000 INFO @ Tue, 02 Aug 2022 10:21:22: 3000000 INFO @ Tue, 02 Aug 2022 10:21:24: 8000000 INFO @ Tue, 02 Aug 2022 10:21:29: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 10:21:31: 9000000 INFO @ Tue, 02 Aug 2022 10:21:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 10:21:32: #1 read tag files... INFO @ Tue, 02 Aug 2022 10:21:32: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 10:21:35: 5000000 INFO @ Tue, 02 Aug 2022 10:21:38: 10000000 INFO @ Tue, 02 Aug 2022 10:21:39: 1000000 INFO @ Tue, 02 Aug 2022 10:21:42: 6000000 INFO @ Tue, 02 Aug 2022 10:21:45: 11000000 INFO @ Tue, 02 Aug 2022 10:21:46: 2000000 INFO @ Tue, 02 Aug 2022 10:21:49: 7000000 INFO @ Tue, 02 Aug 2022 10:21:52: 12000000 INFO @ Tue, 02 Aug 2022 10:21:53: 3000000 INFO @ Tue, 02 Aug 2022 10:21:56: 8000000 INFO @ Tue, 02 Aug 2022 10:21:59: 13000000 INFO @ Tue, 02 Aug 2022 10:22:00: 4000000 INFO @ Tue, 02 Aug 2022 10:22:02: 9000000 INFO @ Tue, 02 Aug 2022 10:22:06: 14000000 INFO @ Tue, 02 Aug 2022 10:22:07: 5000000 INFO @ Tue, 02 Aug 2022 10:22:09: 10000000 INFO @ Tue, 02 Aug 2022 10:22:13: 15000000 INFO @ Tue, 02 Aug 2022 10:22:14: 6000000 INFO @ Tue, 02 Aug 2022 10:22:16: 11000000 INFO @ Tue, 02 Aug 2022 10:22:20: 16000000 INFO @ Tue, 02 Aug 2022 10:22:21: 7000000 INFO @ Tue, 02 Aug 2022 10:22:23: 12000000 INFO @ Tue, 02 Aug 2022 10:22:27: 17000000 INFO @ Tue, 02 Aug 2022 10:22:28: 8000000 INFO @ Tue, 02 Aug 2022 10:22:30: 13000000 INFO @ Tue, 02 Aug 2022 10:22:34: 18000000 INFO @ Tue, 02 Aug 2022 10:22:35: 9000000 INFO @ Tue, 02 Aug 2022 10:22:36: 14000000 INFO @ Tue, 02 Aug 2022 10:22:41: 19000000 INFO @ Tue, 02 Aug 2022 10:22:42: 10000000 INFO @ Tue, 02 Aug 2022 10:22:43: 15000000 INFO @ Tue, 02 Aug 2022 10:22:48: 20000000 INFO @ Tue, 02 Aug 2022 10:22:49: 11000000 INFO @ Tue, 02 Aug 2022 10:22:50: 16000000 INFO @ Tue, 02 Aug 2022 10:22:55: 21000000 INFO @ Tue, 02 Aug 2022 10:22:56: 12000000 INFO @ Tue, 02 Aug 2022 10:22:57: 17000000 INFO @ Tue, 02 Aug 2022 10:23:02: 22000000 INFO @ Tue, 02 Aug 2022 10:23:03: 13000000 INFO @ Tue, 02 Aug 2022 10:23:03: 18000000 INFO @ Tue, 02 Aug 2022 10:23:09: 23000000 INFO @ Tue, 02 Aug 2022 10:23:10: 19000000 INFO @ Tue, 02 Aug 2022 10:23:10: 14000000 INFO @ Tue, 02 Aug 2022 10:23:16: 24000000 INFO @ Tue, 02 Aug 2022 10:23:17: 20000000 INFO @ Tue, 02 Aug 2022 10:23:17: 15000000 INFO @ Tue, 02 Aug 2022 10:23:22: 25000000 INFO @ Tue, 02 Aug 2022 10:23:24: 21000000 INFO @ Tue, 02 Aug 2022 10:23:24: 16000000 INFO @ Tue, 02 Aug 2022 10:23:29: 26000000 INFO @ Tue, 02 Aug 2022 10:23:30: 22000000 INFO @ Tue, 02 Aug 2022 10:23:31: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 10:23:36: 27000000 INFO @ Tue, 02 Aug 2022 10:23:37: 23000000 INFO @ Tue, 02 Aug 2022 10:23:38: 18000000 INFO @ Tue, 02 Aug 2022 10:23:43: 28000000 INFO @ Tue, 02 Aug 2022 10:23:44: 24000000 INFO @ Tue, 02 Aug 2022 10:23:45: 19000000 INFO @ Tue, 02 Aug 2022 10:23:49: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 10:23:49: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 10:23:49: #1 total tags in treatment: 28769975 INFO @ Tue, 02 Aug 2022 10:23:49: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 10:23:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 10:23:49: #1 tags after filtering in treatment: 28769975 INFO @ Tue, 02 Aug 2022 10:23:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 10:23:49: #1 finished! INFO @ Tue, 02 Aug 2022 10:23:49: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 10:23:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 10:23:50: 25000000 INFO @ Tue, 02 Aug 2022 10:23:51: #2 number of paired peaks: 85 WARNING @ Tue, 02 Aug 2022 10:23:51: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 10:23:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 10:23:52: 20000000 INFO @ Tue, 02 Aug 2022 10:23:57: 26000000 INFO @ Tue, 02 Aug 2022 10:23:59: 21000000 INFO @ Tue, 02 Aug 2022 10:24:04: 27000000 INFO @ Tue, 02 Aug 2022 10:24:06: 22000000 INFO @ Tue, 02 Aug 2022 10:24:11: 28000000 INFO @ Tue, 02 Aug 2022 10:24:13: 23000000 INFO @ Tue, 02 Aug 2022 10:24:16: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 10:24:16: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 10:24:16: #1 total tags in treatment: 28769975 INFO @ Tue, 02 Aug 2022 10:24:16: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 10:24:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 10:24:16: #1 tags after filtering in treatment: 28769975 INFO @ Tue, 02 Aug 2022 10:24:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 10:24:16: #1 finished! INFO @ Tue, 02 Aug 2022 10:24:16: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 10:24:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 10:24:18: #2 number of paired peaks: 85 WARNING @ Tue, 02 Aug 2022 10:24:18: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 10:24:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 10:24:20: 24000000 INFO @ Tue, 02 Aug 2022 10:24:26: 25000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 10:24:33: 26000000 INFO @ Tue, 02 Aug 2022 10:24:39: 27000000 INFO @ Tue, 02 Aug 2022 10:24:45: 28000000 INFO @ Tue, 02 Aug 2022 10:24:50: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 10:24:50: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 10:24:50: #1 total tags in treatment: 28769975 INFO @ Tue, 02 Aug 2022 10:24:50: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 10:24:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 10:24:50: #1 tags after filtering in treatment: 28769975 INFO @ Tue, 02 Aug 2022 10:24:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 10:24:50: #1 finished! INFO @ Tue, 02 Aug 2022 10:24:50: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 10:24:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 10:24:52: #2 number of paired peaks: 85 WARNING @ Tue, 02 Aug 2022 10:24:52: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 10:24:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10435518/SRX10435518.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling