Job ID = 16434839 SRX = SRX10435513 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 31057573 spots for SRR14060945/SRR14060945.sra Written 31057573 spots for SRR14060945/SRR14060945.sra fastq に変換しました。 bowtie でマッピング中... Your job 16434901 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:15 31057573 reads; of these: 31057573 (100.00%) were unpaired; of these: 1651314 (5.32%) aligned 0 times 25439506 (81.91%) aligned exactly 1 time 3966753 (12.77%) aligned >1 times 94.68% overall alignment rate Time searching: 00:09:15 Overall time: 00:09:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5381554 / 29406259 = 0.1830 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 10:15:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 10:15:05: #1 read tag files... INFO @ Tue, 02 Aug 2022 10:15:05: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 10:15:11: 1000000 INFO @ Tue, 02 Aug 2022 10:15:16: 2000000 INFO @ Tue, 02 Aug 2022 10:15:21: 3000000 INFO @ Tue, 02 Aug 2022 10:15:26: 4000000 INFO @ Tue, 02 Aug 2022 10:15:32: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 10:15:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 10:15:34: #1 read tag files... INFO @ Tue, 02 Aug 2022 10:15:34: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 10:15:38: 6000000 INFO @ Tue, 02 Aug 2022 10:15:40: 1000000 INFO @ Tue, 02 Aug 2022 10:15:43: 7000000 INFO @ Tue, 02 Aug 2022 10:15:46: 2000000 INFO @ Tue, 02 Aug 2022 10:15:49: 8000000 INFO @ Tue, 02 Aug 2022 10:15:52: 3000000 INFO @ Tue, 02 Aug 2022 10:15:55: 9000000 INFO @ Tue, 02 Aug 2022 10:15:58: 4000000 INFO @ Tue, 02 Aug 2022 10:16:01: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 10:16:04: 5000000 INFO @ Tue, 02 Aug 2022 10:16:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 10:16:04: #1 read tag files... INFO @ Tue, 02 Aug 2022 10:16:04: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 10:16:07: 11000000 INFO @ Tue, 02 Aug 2022 10:16:10: 6000000 INFO @ Tue, 02 Aug 2022 10:16:11: 1000000 INFO @ Tue, 02 Aug 2022 10:16:13: 12000000 INFO @ Tue, 02 Aug 2022 10:16:16: 7000000 INFO @ Tue, 02 Aug 2022 10:16:18: 2000000 INFO @ Tue, 02 Aug 2022 10:16:19: 13000000 INFO @ Tue, 02 Aug 2022 10:16:22: 8000000 INFO @ Tue, 02 Aug 2022 10:16:24: 3000000 INFO @ Tue, 02 Aug 2022 10:16:25: 14000000 INFO @ Tue, 02 Aug 2022 10:16:28: 9000000 INFO @ Tue, 02 Aug 2022 10:16:31: 4000000 INFO @ Tue, 02 Aug 2022 10:16:31: 15000000 INFO @ Tue, 02 Aug 2022 10:16:34: 10000000 INFO @ Tue, 02 Aug 2022 10:16:37: 16000000 INFO @ Tue, 02 Aug 2022 10:16:37: 5000000 INFO @ Tue, 02 Aug 2022 10:16:40: 11000000 INFO @ Tue, 02 Aug 2022 10:16:43: 17000000 INFO @ Tue, 02 Aug 2022 10:16:44: 6000000 INFO @ Tue, 02 Aug 2022 10:16:46: 12000000 INFO @ Tue, 02 Aug 2022 10:16:49: 18000000 INFO @ Tue, 02 Aug 2022 10:16:50: 7000000 INFO @ Tue, 02 Aug 2022 10:16:52: 13000000 INFO @ Tue, 02 Aug 2022 10:16:55: 19000000 INFO @ Tue, 02 Aug 2022 10:16:57: 8000000 INFO @ Tue, 02 Aug 2022 10:16:58: 14000000 INFO @ Tue, 02 Aug 2022 10:17:01: 20000000 INFO @ Tue, 02 Aug 2022 10:17:04: 9000000 INFO @ Tue, 02 Aug 2022 10:17:04: 15000000 INFO @ Tue, 02 Aug 2022 10:17:07: 21000000 INFO @ Tue, 02 Aug 2022 10:17:10: 16000000 INFO @ Tue, 02 Aug 2022 10:17:10: 10000000 INFO @ Tue, 02 Aug 2022 10:17:14: 22000000 INFO @ Tue, 02 Aug 2022 10:17:16: 17000000 INFO @ Tue, 02 Aug 2022 10:17:17: 11000000 INFO @ Tue, 02 Aug 2022 10:17:20: 23000000 INFO @ Tue, 02 Aug 2022 10:17:22: 18000000 INFO @ Tue, 02 Aug 2022 10:17:23: 12000000 INFO @ Tue, 02 Aug 2022 10:17:26: 24000000 INFO @ Tue, 02 Aug 2022 10:17:26: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 10:17:26: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 10:17:26: #1 total tags in treatment: 24024705 INFO @ Tue, 02 Aug 2022 10:17:26: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 10:17:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 10:17:26: #1 tags after filtering in treatment: 24024705 INFO @ Tue, 02 Aug 2022 10:17:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 10:17:26: #1 finished! INFO @ Tue, 02 Aug 2022 10:17:26: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 10:17:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 10:17:28: #2 number of paired peaks: 99 WARNING @ Tue, 02 Aug 2022 10:17:28: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 10:17:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.05_peaks.narrowPeak: No such file or directory INFO @ Tue, 02 Aug 2022 10:17:28: 19000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 10:17:30: 13000000 INFO @ Tue, 02 Aug 2022 10:17:34: 20000000 INFO @ Tue, 02 Aug 2022 10:17:37: 14000000 INFO @ Tue, 02 Aug 2022 10:17:40: 21000000 INFO @ Tue, 02 Aug 2022 10:17:43: 15000000 INFO @ Tue, 02 Aug 2022 10:17:46: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 10:17:50: 16000000 INFO @ Tue, 02 Aug 2022 10:17:52: 23000000 INFO @ Tue, 02 Aug 2022 10:17:56: 17000000 INFO @ Tue, 02 Aug 2022 10:17:58: 24000000 INFO @ Tue, 02 Aug 2022 10:17:58: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 10:17:58: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 10:17:58: #1 total tags in treatment: 24024705 INFO @ Tue, 02 Aug 2022 10:17:58: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 10:17:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 10:17:58: #1 tags after filtering in treatment: 24024705 INFO @ Tue, 02 Aug 2022 10:17:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 10:17:58: #1 finished! INFO @ Tue, 02 Aug 2022 10:17:58: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 10:17:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 10:18:00: #2 number of paired peaks: 99 WARNING @ Tue, 02 Aug 2022 10:18:00: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 10:18:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 10:18:03: 18000000 INFO @ Tue, 02 Aug 2022 10:18:09: 19000000 INFO @ Tue, 02 Aug 2022 10:18:15: 20000000 INFO @ Tue, 02 Aug 2022 10:18:21: 21000000 INFO @ Tue, 02 Aug 2022 10:18:28: 22000000 INFO @ Tue, 02 Aug 2022 10:18:34: 23000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 10:18:40: 24000000 INFO @ Tue, 02 Aug 2022 10:18:40: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 10:18:40: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 10:18:40: #1 total tags in treatment: 24024705 INFO @ Tue, 02 Aug 2022 10:18:40: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 10:18:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 10:18:41: #1 tags after filtering in treatment: 24024705 INFO @ Tue, 02 Aug 2022 10:18:41: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 10:18:41: #1 finished! INFO @ Tue, 02 Aug 2022 10:18:41: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 10:18:41: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 10:18:42: #2 number of paired peaks: 99 WARNING @ Tue, 02 Aug 2022 10:18:42: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 10:18:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10435513/SRX10435513.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling