Job ID = 14160584 SRX = SRX10399025 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 23953629 spots for SRR14022413/SRR14022413.sra Written 23953629 spots for SRR14022413/SRR14022413.sra fastq に変換しました。 bowtie でマッピング中... Your job 14160760 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:11 23953629 reads; of these: 23953629 (100.00%) were unpaired; of these: 23938674 (99.94%) aligned 0 times 9984 (0.04%) aligned exactly 1 time 4971 (0.02%) aligned >1 times 0.06% overall alignment rate Time searching: 00:16:11 Overall time: 00:16:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 4680 / 14955 = 0.3129 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:35:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:35:15: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:35:15: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:35:15: #1 tag size is determined as 152 bps INFO @ Thu, 09 Dec 2021 03:35:15: #1 tag size = 152 INFO @ Thu, 09 Dec 2021 03:35:15: #1 total tags in treatment: 10275 INFO @ Thu, 09 Dec 2021 03:35:15: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:35:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:35:15: #1 tags after filtering in treatment: 10275 INFO @ Thu, 09 Dec 2021 03:35:15: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:35:15: #1 finished! INFO @ Thu, 09 Dec 2021 03:35:15: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:35:15: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:35:16: #2 number of paired peaks: 463 WARNING @ Thu, 09 Dec 2021 03:35:16: Fewer paired peaks (463) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 463 pairs to build model! INFO @ Thu, 09 Dec 2021 03:35:16: start model_add_line... INFO @ Thu, 09 Dec 2021 03:35:16: start X-correlation... INFO @ Thu, 09 Dec 2021 03:35:16: end of X-cor INFO @ Thu, 09 Dec 2021 03:35:16: #2 finished! INFO @ Thu, 09 Dec 2021 03:35:16: #2 predicted fragment length is 239 bps INFO @ Thu, 09 Dec 2021 03:35:16: #2 alternative fragment length(s) may be 10,239 bps INFO @ Thu, 09 Dec 2021 03:35:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.05_model.r WARNING @ Thu, 09 Dec 2021 03:35:16: #2 Since the d (239) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 03:35:16: #2 You may need to consider one of the other alternative d(s): 10,239 WARNING @ Thu, 09 Dec 2021 03:35:16: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 03:35:16: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:35:16: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 03:35:16: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:35:16: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.05_peaks.xls INFO @ Thu, 09 Dec 2021 03:35:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:35:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.05_summits.bed INFO @ Thu, 09 Dec 2021 03:35:16: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (37 records, 4 fields): 2 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:35:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:35:45: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:35:45: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:35:45: #1 tag size is determined as 152 bps INFO @ Thu, 09 Dec 2021 03:35:45: #1 tag size = 152 INFO @ Thu, 09 Dec 2021 03:35:45: #1 total tags in treatment: 10275 INFO @ Thu, 09 Dec 2021 03:35:45: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:35:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:35:45: #1 tags after filtering in treatment: 10275 INFO @ Thu, 09 Dec 2021 03:35:45: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:35:45: #1 finished! INFO @ Thu, 09 Dec 2021 03:35:45: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:35:45: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:35:46: #2 number of paired peaks: 463 WARNING @ Thu, 09 Dec 2021 03:35:46: Fewer paired peaks (463) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 463 pairs to build model! INFO @ Thu, 09 Dec 2021 03:35:46: start model_add_line... INFO @ Thu, 09 Dec 2021 03:35:46: start X-correlation... INFO @ Thu, 09 Dec 2021 03:35:46: end of X-cor INFO @ Thu, 09 Dec 2021 03:35:46: #2 finished! INFO @ Thu, 09 Dec 2021 03:35:46: #2 predicted fragment length is 239 bps INFO @ Thu, 09 Dec 2021 03:35:46: #2 alternative fragment length(s) may be 10,239 bps INFO @ Thu, 09 Dec 2021 03:35:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.10_model.r WARNING @ Thu, 09 Dec 2021 03:35:46: #2 Since the d (239) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 03:35:46: #2 You may need to consider one of the other alternative d(s): 10,239 WARNING @ Thu, 09 Dec 2021 03:35:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 03:35:46: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:35:46: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 03:35:46: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:35:46: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.10_peaks.xls INFO @ Thu, 09 Dec 2021 03:35:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:35:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.10_summits.bed INFO @ Thu, 09 Dec 2021 03:35:46: Done! pass1 - making usageList (5 chroms): 0 millis pass2 - checking and writing primary data (11 records, 4 fields): 9 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 03:36:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:36:16: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:36:16: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:36:16: #1 tag size is determined as 152 bps INFO @ Thu, 09 Dec 2021 03:36:16: #1 tag size = 152 INFO @ Thu, 09 Dec 2021 03:36:16: #1 total tags in treatment: 10275 INFO @ Thu, 09 Dec 2021 03:36:16: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:36:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:36:16: #1 tags after filtering in treatment: 10275 INFO @ Thu, 09 Dec 2021 03:36:16: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:36:16: #1 finished! INFO @ Thu, 09 Dec 2021 03:36:16: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:36:16: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:36:16: #2 number of paired peaks: 463 WARNING @ Thu, 09 Dec 2021 03:36:16: Fewer paired peaks (463) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 463 pairs to build model! INFO @ Thu, 09 Dec 2021 03:36:16: start model_add_line... INFO @ Thu, 09 Dec 2021 03:36:16: start X-correlation... INFO @ Thu, 09 Dec 2021 03:36:16: end of X-cor INFO @ Thu, 09 Dec 2021 03:36:16: #2 finished! INFO @ Thu, 09 Dec 2021 03:36:16: #2 predicted fragment length is 239 bps INFO @ Thu, 09 Dec 2021 03:36:16: #2 alternative fragment length(s) may be 10,239 bps INFO @ Thu, 09 Dec 2021 03:36:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.20_model.r WARNING @ Thu, 09 Dec 2021 03:36:16: #2 Since the d (239) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 03:36:16: #2 You may need to consider one of the other alternative d(s): 10,239 WARNING @ Thu, 09 Dec 2021 03:36:16: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 03:36:16: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:36:16: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 03:36:16: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:36:16: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.20_peaks.xls INFO @ Thu, 09 Dec 2021 03:36:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:36:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX10399025/SRX10399025.20_summits.bed INFO @ Thu, 09 Dec 2021 03:36:16: Done! pass1 - making usageList (5 chroms): 1 millis pass2 - checking and writing primary data (5 records, 4 fields): 1 millis CompletedMACS2peakCalling