Job ID = 14157895 SRX = SRX10040021 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 56175430 spots for SRR13642800/SRR13642800.sra Written 56175430 spots for SRR13642800/SRR13642800.sra fastq に変換しました。 bowtie でマッピング中... Your job 14158102 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:36 56175430 reads; of these: 56175430 (100.00%) were unpaired; of these: 8943101 (15.92%) aligned 0 times 38633206 (68.77%) aligned exactly 1 time 8599123 (15.31%) aligned >1 times 84.08% overall alignment rate Time searching: 00:10:36 Overall time: 00:10:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8693657 / 47232329 = 0.1841 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 12:45:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 12:45:32: #1 read tag files... INFO @ Wed, 08 Dec 2021 12:45:32: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 12:45:38: 1000000 INFO @ Wed, 08 Dec 2021 12:45:43: 2000000 INFO @ Wed, 08 Dec 2021 12:45:48: 3000000 INFO @ Wed, 08 Dec 2021 12:45:53: 4000000 INFO @ Wed, 08 Dec 2021 12:45:58: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 12:46:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 12:46:02: #1 read tag files... INFO @ Wed, 08 Dec 2021 12:46:02: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 12:46:03: 6000000 INFO @ Wed, 08 Dec 2021 12:46:07: 1000000 INFO @ Wed, 08 Dec 2021 12:46:09: 7000000 INFO @ Wed, 08 Dec 2021 12:46:13: 2000000 INFO @ Wed, 08 Dec 2021 12:46:14: 8000000 INFO @ Wed, 08 Dec 2021 12:46:18: 3000000 INFO @ Wed, 08 Dec 2021 12:46:19: 9000000 INFO @ Wed, 08 Dec 2021 12:46:23: 4000000 INFO @ Wed, 08 Dec 2021 12:46:24: 10000000 INFO @ Wed, 08 Dec 2021 12:46:28: 5000000 INFO @ Wed, 08 Dec 2021 12:46:29: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 12:46:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 12:46:32: #1 read tag files... INFO @ Wed, 08 Dec 2021 12:46:32: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 12:46:33: 6000000 INFO @ Wed, 08 Dec 2021 12:46:34: 12000000 INFO @ Wed, 08 Dec 2021 12:46:38: 1000000 INFO @ Wed, 08 Dec 2021 12:46:39: 7000000 INFO @ Wed, 08 Dec 2021 12:46:39: 13000000 INFO @ Wed, 08 Dec 2021 12:46:44: 2000000 INFO @ Wed, 08 Dec 2021 12:46:44: 8000000 INFO @ Wed, 08 Dec 2021 12:46:45: 14000000 INFO @ Wed, 08 Dec 2021 12:46:49: 9000000 INFO @ Wed, 08 Dec 2021 12:46:50: 15000000 INFO @ Wed, 08 Dec 2021 12:46:50: 3000000 INFO @ Wed, 08 Dec 2021 12:46:55: 10000000 INFO @ Wed, 08 Dec 2021 12:46:55: 16000000 INFO @ Wed, 08 Dec 2021 12:46:57: 4000000 INFO @ Wed, 08 Dec 2021 12:47:00: 11000000 INFO @ Wed, 08 Dec 2021 12:47:01: 17000000 INFO @ Wed, 08 Dec 2021 12:47:03: 5000000 INFO @ Wed, 08 Dec 2021 12:47:05: 12000000 INFO @ Wed, 08 Dec 2021 12:47:06: 18000000 INFO @ Wed, 08 Dec 2021 12:47:09: 6000000 INFO @ Wed, 08 Dec 2021 12:47:11: 13000000 INFO @ Wed, 08 Dec 2021 12:47:11: 19000000 INFO @ Wed, 08 Dec 2021 12:47:15: 7000000 INFO @ Wed, 08 Dec 2021 12:47:16: 14000000 INFO @ Wed, 08 Dec 2021 12:47:17: 20000000 INFO @ Wed, 08 Dec 2021 12:47:21: 15000000 INFO @ Wed, 08 Dec 2021 12:47:21: 8000000 INFO @ Wed, 08 Dec 2021 12:47:22: 21000000 INFO @ Wed, 08 Dec 2021 12:47:27: 16000000 INFO @ Wed, 08 Dec 2021 12:47:27: 22000000 INFO @ Wed, 08 Dec 2021 12:47:27: 9000000 INFO @ Wed, 08 Dec 2021 12:47:32: 17000000 INFO @ Wed, 08 Dec 2021 12:47:33: 23000000 INFO @ Wed, 08 Dec 2021 12:47:33: 10000000 INFO @ Wed, 08 Dec 2021 12:47:37: 18000000 INFO @ Wed, 08 Dec 2021 12:47:38: 24000000 INFO @ Wed, 08 Dec 2021 12:47:38: 11000000 INFO @ Wed, 08 Dec 2021 12:47:43: 19000000 INFO @ Wed, 08 Dec 2021 12:47:43: 25000000 INFO @ Wed, 08 Dec 2021 12:47:44: 12000000 INFO @ Wed, 08 Dec 2021 12:47:48: 20000000 INFO @ Wed, 08 Dec 2021 12:47:49: 26000000 INFO @ Wed, 08 Dec 2021 12:47:50: 13000000 INFO @ Wed, 08 Dec 2021 12:47:53: 21000000 INFO @ Wed, 08 Dec 2021 12:47:54: 27000000 INFO @ Wed, 08 Dec 2021 12:47:56: 14000000 INFO @ Wed, 08 Dec 2021 12:47:59: 22000000 INFO @ Wed, 08 Dec 2021 12:47:59: 28000000 INFO @ Wed, 08 Dec 2021 12:48:02: 15000000 INFO @ Wed, 08 Dec 2021 12:48:04: 23000000 INFO @ Wed, 08 Dec 2021 12:48:04: 29000000 INFO @ Wed, 08 Dec 2021 12:48:08: 16000000 INFO @ Wed, 08 Dec 2021 12:48:09: 24000000 INFO @ Wed, 08 Dec 2021 12:48:10: 30000000 INFO @ Wed, 08 Dec 2021 12:48:14: 17000000 INFO @ Wed, 08 Dec 2021 12:48:15: 25000000 INFO @ Wed, 08 Dec 2021 12:48:15: 31000000 INFO @ Wed, 08 Dec 2021 12:48:20: 26000000 INFO @ Wed, 08 Dec 2021 12:48:20: 18000000 INFO @ Wed, 08 Dec 2021 12:48:20: 32000000 INFO @ Wed, 08 Dec 2021 12:48:25: 27000000 INFO @ Wed, 08 Dec 2021 12:48:26: 33000000 INFO @ Wed, 08 Dec 2021 12:48:26: 19000000 INFO @ Wed, 08 Dec 2021 12:48:30: 28000000 INFO @ Wed, 08 Dec 2021 12:48:31: 34000000 INFO @ Wed, 08 Dec 2021 12:48:32: 20000000 INFO @ Wed, 08 Dec 2021 12:48:36: 29000000 INFO @ Wed, 08 Dec 2021 12:48:36: 35000000 INFO @ Wed, 08 Dec 2021 12:48:38: 21000000 INFO @ Wed, 08 Dec 2021 12:48:41: 30000000 INFO @ Wed, 08 Dec 2021 12:48:42: 36000000 INFO @ Wed, 08 Dec 2021 12:48:44: 22000000 INFO @ Wed, 08 Dec 2021 12:48:46: 31000000 INFO @ Wed, 08 Dec 2021 12:48:47: 37000000 INFO @ Wed, 08 Dec 2021 12:48:50: 23000000 INFO @ Wed, 08 Dec 2021 12:48:51: 32000000 INFO @ Wed, 08 Dec 2021 12:48:52: 38000000 INFO @ Wed, 08 Dec 2021 12:48:55: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 12:48:55: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 12:48:55: #1 total tags in treatment: 38538672 INFO @ Wed, 08 Dec 2021 12:48:55: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 12:48:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 12:48:56: 24000000 INFO @ Wed, 08 Dec 2021 12:48:56: #1 tags after filtering in treatment: 38538672 INFO @ Wed, 08 Dec 2021 12:48:56: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 12:48:56: #1 finished! INFO @ Wed, 08 Dec 2021 12:48:56: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 12:48:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 12:48:57: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 12:48:58: #2 number of paired peaks: 0 WARNING @ Wed, 08 Dec 2021 12:48:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 12:48:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 12:49:02: 25000000 INFO @ Wed, 08 Dec 2021 12:49:02: 34000000 INFO @ Wed, 08 Dec 2021 12:49:07: 35000000 INFO @ Wed, 08 Dec 2021 12:49:07: 26000000 INFO @ Wed, 08 Dec 2021 12:49:12: 36000000 INFO @ Wed, 08 Dec 2021 12:49:13: 27000000 INFO @ Wed, 08 Dec 2021 12:49:18: 37000000 INFO @ Wed, 08 Dec 2021 12:49:19: 28000000 INFO @ Wed, 08 Dec 2021 12:49:23: 38000000 INFO @ Wed, 08 Dec 2021 12:49:25: 29000000 INFO @ Wed, 08 Dec 2021 12:49:26: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 12:49:26: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 12:49:26: #1 total tags in treatment: 38538672 INFO @ Wed, 08 Dec 2021 12:49:26: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 12:49:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 12:49:27: #1 tags after filtering in treatment: 38538672 INFO @ Wed, 08 Dec 2021 12:49:27: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 12:49:27: #1 finished! INFO @ Wed, 08 Dec 2021 12:49:27: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 12:49:27: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 12:49:29: #2 number of paired peaks: 0 WARNING @ Wed, 08 Dec 2021 12:49:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 12:49:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 12:49:31: 30000000 INFO @ Wed, 08 Dec 2021 12:49:37: 31000000 INFO @ Wed, 08 Dec 2021 12:49:42: 32000000 INFO @ Wed, 08 Dec 2021 12:49:48: 33000000 INFO @ Wed, 08 Dec 2021 12:49:53: 34000000 INFO @ Wed, 08 Dec 2021 12:49:58: 35000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 12:50:04: 36000000 INFO @ Wed, 08 Dec 2021 12:50:09: 37000000 INFO @ Wed, 08 Dec 2021 12:50:15: 38000000 INFO @ Wed, 08 Dec 2021 12:50:18: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 12:50:18: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 12:50:18: #1 total tags in treatment: 38538672 INFO @ Wed, 08 Dec 2021 12:50:18: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 12:50:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 12:50:19: #1 tags after filtering in treatment: 38538672 INFO @ Wed, 08 Dec 2021 12:50:19: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 12:50:19: #1 finished! INFO @ Wed, 08 Dec 2021 12:50:19: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 12:50:19: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 12:50:21: #2 number of paired peaks: 0 WARNING @ Wed, 08 Dec 2021 12:50:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 12:50:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040021/SRX10040021.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling