Job ID = 14157887 SRX = SRX10040018 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 50621464 spots for SRR13642803/SRR13642803.sra Written 50621464 spots for SRR13642803/SRR13642803.sra fastq に変換しました。 bowtie でマッピング中... Your job 14158090 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:50 50621464 reads; of these: 50621464 (100.00%) were unpaired; of these: 3266983 (6.45%) aligned 0 times 36197716 (71.51%) aligned exactly 1 time 11156765 (22.04%) aligned >1 times 93.55% overall alignment rate Time searching: 00:09:50 Overall time: 00:09:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 12264127 / 47354481 = 0.2590 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 12:35:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 12:35:20: #1 read tag files... INFO @ Wed, 08 Dec 2021 12:35:20: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 12:35:25: 1000000 INFO @ Wed, 08 Dec 2021 12:35:29: 2000000 INFO @ Wed, 08 Dec 2021 12:35:34: 3000000 INFO @ Wed, 08 Dec 2021 12:35:39: 4000000 INFO @ Wed, 08 Dec 2021 12:35:44: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 12:35:48: 6000000 INFO @ Wed, 08 Dec 2021 12:35:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 12:35:50: #1 read tag files... INFO @ Wed, 08 Dec 2021 12:35:50: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 12:35:53: 7000000 INFO @ Wed, 08 Dec 2021 12:35:56: 1000000 INFO @ Wed, 08 Dec 2021 12:35:59: 8000000 INFO @ Wed, 08 Dec 2021 12:36:02: 2000000 INFO @ Wed, 08 Dec 2021 12:36:04: 9000000 INFO @ Wed, 08 Dec 2021 12:36:08: 3000000 INFO @ Wed, 08 Dec 2021 12:36:09: 10000000 INFO @ Wed, 08 Dec 2021 12:36:14: 4000000 INFO @ Wed, 08 Dec 2021 12:36:14: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 12:36:19: 12000000 INFO @ Wed, 08 Dec 2021 12:36:20: 5000000 INFO @ Wed, 08 Dec 2021 12:36:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 12:36:20: #1 read tag files... INFO @ Wed, 08 Dec 2021 12:36:20: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 12:36:25: 13000000 INFO @ Wed, 08 Dec 2021 12:36:25: 1000000 INFO @ Wed, 08 Dec 2021 12:36:25: 6000000 INFO @ Wed, 08 Dec 2021 12:36:30: 14000000 INFO @ Wed, 08 Dec 2021 12:36:31: 2000000 INFO @ Wed, 08 Dec 2021 12:36:31: 7000000 INFO @ Wed, 08 Dec 2021 12:36:36: 15000000 INFO @ Wed, 08 Dec 2021 12:36:36: 3000000 INFO @ Wed, 08 Dec 2021 12:36:37: 8000000 INFO @ Wed, 08 Dec 2021 12:36:41: 16000000 INFO @ Wed, 08 Dec 2021 12:36:42: 4000000 INFO @ Wed, 08 Dec 2021 12:36:43: 9000000 INFO @ Wed, 08 Dec 2021 12:36:47: 17000000 INFO @ Wed, 08 Dec 2021 12:36:47: 5000000 INFO @ Wed, 08 Dec 2021 12:36:49: 10000000 INFO @ Wed, 08 Dec 2021 12:36:52: 18000000 INFO @ Wed, 08 Dec 2021 12:36:53: 6000000 INFO @ Wed, 08 Dec 2021 12:36:55: 11000000 INFO @ Wed, 08 Dec 2021 12:36:57: 19000000 INFO @ Wed, 08 Dec 2021 12:36:58: 7000000 INFO @ Wed, 08 Dec 2021 12:37:01: 12000000 INFO @ Wed, 08 Dec 2021 12:37:03: 20000000 INFO @ Wed, 08 Dec 2021 12:37:03: 8000000 INFO @ Wed, 08 Dec 2021 12:37:07: 13000000 INFO @ Wed, 08 Dec 2021 12:37:08: 21000000 INFO @ Wed, 08 Dec 2021 12:37:08: 9000000 INFO @ Wed, 08 Dec 2021 12:37:12: 14000000 INFO @ Wed, 08 Dec 2021 12:37:13: 22000000 INFO @ Wed, 08 Dec 2021 12:37:14: 10000000 INFO @ Wed, 08 Dec 2021 12:37:18: 15000000 INFO @ Wed, 08 Dec 2021 12:37:18: 23000000 INFO @ Wed, 08 Dec 2021 12:37:19: 11000000 INFO @ Wed, 08 Dec 2021 12:37:24: 24000000 INFO @ Wed, 08 Dec 2021 12:37:24: 16000000 INFO @ Wed, 08 Dec 2021 12:37:24: 12000000 INFO @ Wed, 08 Dec 2021 12:37:29: 25000000 INFO @ Wed, 08 Dec 2021 12:37:30: 13000000 INFO @ Wed, 08 Dec 2021 12:37:30: 17000000 INFO @ Wed, 08 Dec 2021 12:37:34: 26000000 INFO @ Wed, 08 Dec 2021 12:37:35: 14000000 INFO @ Wed, 08 Dec 2021 12:37:36: 18000000 INFO @ Wed, 08 Dec 2021 12:37:39: 27000000 INFO @ Wed, 08 Dec 2021 12:37:40: 15000000 INFO @ Wed, 08 Dec 2021 12:37:42: 19000000 INFO @ Wed, 08 Dec 2021 12:37:45: 28000000 INFO @ Wed, 08 Dec 2021 12:37:45: 16000000 INFO @ Wed, 08 Dec 2021 12:37:47: 20000000 INFO @ Wed, 08 Dec 2021 12:37:50: 29000000 INFO @ Wed, 08 Dec 2021 12:37:51: 17000000 INFO @ Wed, 08 Dec 2021 12:37:53: 21000000 INFO @ Wed, 08 Dec 2021 12:37:55: 30000000 INFO @ Wed, 08 Dec 2021 12:37:56: 18000000 INFO @ Wed, 08 Dec 2021 12:37:59: 22000000 INFO @ Wed, 08 Dec 2021 12:38:01: 31000000 INFO @ Wed, 08 Dec 2021 12:38:01: 19000000 INFO @ Wed, 08 Dec 2021 12:38:05: 23000000 INFO @ Wed, 08 Dec 2021 12:38:06: 32000000 INFO @ Wed, 08 Dec 2021 12:38:06: 20000000 INFO @ Wed, 08 Dec 2021 12:38:11: 24000000 INFO @ Wed, 08 Dec 2021 12:38:11: 33000000 INFO @ Wed, 08 Dec 2021 12:38:12: 21000000 INFO @ Wed, 08 Dec 2021 12:38:16: 34000000 INFO @ Wed, 08 Dec 2021 12:38:17: 25000000 INFO @ Wed, 08 Dec 2021 12:38:17: 22000000 INFO @ Wed, 08 Dec 2021 12:38:22: 35000000 INFO @ Wed, 08 Dec 2021 12:38:22: 23000000 INFO @ Wed, 08 Dec 2021 12:38:22: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 12:38:22: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 12:38:22: #1 total tags in treatment: 35090354 INFO @ Wed, 08 Dec 2021 12:38:22: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 12:38:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 12:38:22: 26000000 INFO @ Wed, 08 Dec 2021 12:38:23: #1 tags after filtering in treatment: 35090354 INFO @ Wed, 08 Dec 2021 12:38:23: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 12:38:23: #1 finished! INFO @ Wed, 08 Dec 2021 12:38:23: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 12:38:23: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 12:38:25: #2 number of paired peaks: 24 WARNING @ Wed, 08 Dec 2021 12:38:25: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 12:38:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 12:38:27: 24000000 INFO @ Wed, 08 Dec 2021 12:38:28: 27000000 INFO @ Wed, 08 Dec 2021 12:38:32: 25000000 INFO @ Wed, 08 Dec 2021 12:38:34: 28000000 INFO @ Wed, 08 Dec 2021 12:38:37: 26000000 INFO @ Wed, 08 Dec 2021 12:38:40: 29000000 INFO @ Wed, 08 Dec 2021 12:38:42: 27000000 INFO @ Wed, 08 Dec 2021 12:38:46: 30000000 INFO @ Wed, 08 Dec 2021 12:38:48: 28000000 INFO @ Wed, 08 Dec 2021 12:38:51: 31000000 INFO @ Wed, 08 Dec 2021 12:38:53: 29000000 INFO @ Wed, 08 Dec 2021 12:38:57: 32000000 INFO @ Wed, 08 Dec 2021 12:38:58: 30000000 INFO @ Wed, 08 Dec 2021 12:39:03: 33000000 INFO @ Wed, 08 Dec 2021 12:39:03: 31000000 INFO @ Wed, 08 Dec 2021 12:39:08: 32000000 INFO @ Wed, 08 Dec 2021 12:39:09: 34000000 INFO @ Wed, 08 Dec 2021 12:39:13: 33000000 INFO @ Wed, 08 Dec 2021 12:39:14: 35000000 INFO @ Wed, 08 Dec 2021 12:39:15: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 12:39:15: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 12:39:15: #1 total tags in treatment: 35090354 INFO @ Wed, 08 Dec 2021 12:39:15: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 12:39:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 12:39:16: #1 tags after filtering in treatment: 35090354 INFO @ Wed, 08 Dec 2021 12:39:16: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 12:39:16: #1 finished! INFO @ Wed, 08 Dec 2021 12:39:16: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 12:39:16: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 12:39:18: #2 number of paired peaks: 24 WARNING @ Wed, 08 Dec 2021 12:39:18: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 12:39:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 12:39:18: 34000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 12:39:23: 35000000 INFO @ Wed, 08 Dec 2021 12:39:24: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 12:39:24: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 12:39:24: #1 total tags in treatment: 35090354 INFO @ Wed, 08 Dec 2021 12:39:24: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 12:39:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 12:39:24: #1 tags after filtering in treatment: 35090354 INFO @ Wed, 08 Dec 2021 12:39:24: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 12:39:24: #1 finished! INFO @ Wed, 08 Dec 2021 12:39:24: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 12:39:24: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 12:39:26: #2 number of paired peaks: 24 WARNING @ Wed, 08 Dec 2021 12:39:26: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 12:39:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX10040018/SRX10040018.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling