Job ID = 2237031 sra ファイルのダウンロード中... Completed: 92763K bytes transferred in 4 seconds (163129K bits/sec), in 1 file, 2 directories. % Total % Received % Xferd Average Speed Time Time Time Current Dload Upload Total Spent Left Speed 0 0 0 0 0 0 0 0 --:--:-- --:--:-- --:--:-- 0 100 17840 0 17840 0 0 24168 0 --:--:-- --:--:-- --:--:-- 32614 100 38010 0 38010 0 0 51434 0 --:--:-- --:--:-- --:--:-- 69361 sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 5774885 spots for /home/okishinya/chipatlas/results/ce10/SRX080086/SRR298906.sra Written 5774885 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:49 5774885 reads; of these: 5774885 (100.00%) were unpaired; of these: 2218895 (38.42%) aligned 0 times 2923453 (50.62%) aligned exactly 1 time 632537 (10.95%) aligned >1 times 61.58% overall alignment rate Time searching: 00:00:49 Overall time: 00:00:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 1147005 / 3555990 = 0.3226 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Thu, 30 Apr 2015 11:25:30: # Command line: callpeak -t SRX080086.bam -f BAM -g ce -n SRX080086.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX080086.05 # format = BAM # ChIP-seq file = ['SRX080086.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Thu, 30 Apr 2015 11:25:30: # Command line: callpeak -t SRX080086.bam -f BAM -g ce -n SRX080086.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX080086.20 # format = BAM # ChIP-seq file = ['SRX080086.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Thu, 30 Apr 2015 11:25:30: #1 read tag files... INFO @ Thu, 30 Apr 2015 11:25:30: # Command line: callpeak -t SRX080086.bam -f BAM -g ce -n SRX080086.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX080086.10 # format = BAM # ChIP-seq file = ['SRX080086.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Thu, 30 Apr 2015 11:25:30: #1 read tag files... INFO @ Thu, 30 Apr 2015 11:25:30: #1 read treatment tags... INFO @ Thu, 30 Apr 2015 11:25:30: #1 read tag files... INFO @ Thu, 30 Apr 2015 11:25:30: #1 read treatment tags... INFO @ Thu, 30 Apr 2015 11:25:30: #1 read treatment tags... INFO @ Thu, 30 Apr 2015 11:25:36: 1000000 INFO @ Thu, 30 Apr 2015 11:25:36: 1000000 INFO @ Thu, 30 Apr 2015 11:25:36: 1000000 INFO @ Thu, 30 Apr 2015 11:25:42: 2000000 INFO @ Thu, 30 Apr 2015 11:25:42: 2000000 INFO @ Thu, 30 Apr 2015 11:25:42: 2000000 INFO @ Thu, 30 Apr 2015 11:25:44: #1 tag size is determined as 32 bps INFO @ Thu, 30 Apr 2015 11:25:44: #1 tag size = 32 INFO @ Thu, 30 Apr 2015 11:25:44: #1 total tags in treatment: 2408985 INFO @ Thu, 30 Apr 2015 11:25:44: #1 user defined the maximum tags... INFO @ Thu, 30 Apr 2015 11:25:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 30 Apr 2015 11:25:45: #1 tags after filtering in treatment: 2408935 INFO @ Thu, 30 Apr 2015 11:25:45: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 30 Apr 2015 11:25:45: #1 finished! INFO @ Thu, 30 Apr 2015 11:25:45: #2 Build Peak Model... INFO @ Thu, 30 Apr 2015 11:25:45: #1 tag size is determined as 32 bps INFO @ Thu, 30 Apr 2015 11:25:45: #1 tag size = 32 INFO @ Thu, 30 Apr 2015 11:25:45: #1 total tags in treatment: 2408985 INFO @ Thu, 30 Apr 2015 11:25:45: #1 user defined the maximum tags... INFO @ Thu, 30 Apr 2015 11:25:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 30 Apr 2015 11:25:45: #1 tag size is determined as 32 bps INFO @ Thu, 30 Apr 2015 11:25:45: #1 tag size = 32 INFO @ Thu, 30 Apr 2015 11:25:45: #1 total tags in treatment: 2408985 INFO @ Thu, 30 Apr 2015 11:25:45: #1 user defined the maximum tags... INFO @ Thu, 30 Apr 2015 11:25:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 30 Apr 2015 11:25:45: #1 tags after filtering in treatment: 2408935 INFO @ Thu, 30 Apr 2015 11:25:45: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 30 Apr 2015 11:25:45: #1 finished! INFO @ Thu, 30 Apr 2015 11:25:45: #2 Build Peak Model... INFO @ Thu, 30 Apr 2015 11:25:45: #1 tags after filtering in treatment: 2408935 INFO @ Thu, 30 Apr 2015 11:25:45: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 30 Apr 2015 11:25:45: #1 finished! INFO @ Thu, 30 Apr 2015 11:25:45: #2 Build Peak Model... INFO @ Thu, 30 Apr 2015 11:25:45: #2 number of paired peaks: 300 WARNING @ Thu, 30 Apr 2015 11:25:45: Fewer paired peaks (300) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 300 pairs to build model! INFO @ Thu, 30 Apr 2015 11:25:45: start model_add_line... INFO @ Thu, 30 Apr 2015 11:25:45: #2 number of paired peaks: 300 WARNING @ Thu, 30 Apr 2015 11:25:45: Fewer paired peaks (300) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 300 pairs to build model! INFO @ Thu, 30 Apr 2015 11:25:45: start model_add_line... INFO @ Thu, 30 Apr 2015 11:25:45: #2 number of paired peaks: 300 WARNING @ Thu, 30 Apr 2015 11:25:45: Fewer paired peaks (300) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 300 pairs to build model! INFO @ Thu, 30 Apr 2015 11:25:45: start model_add_line... INFO @ Thu, 30 Apr 2015 11:25:46: start X-correlation... INFO @ Thu, 30 Apr 2015 11:25:46: end of X-cor INFO @ Thu, 30 Apr 2015 11:25:46: #2 finished! INFO @ Thu, 30 Apr 2015 11:25:46: #2 predicted fragment length is 43 bps INFO @ Thu, 30 Apr 2015 11:25:46: #2 alternative fragment length(s) may be 43,77,112,142,174,209,246,279,307,345,373,447,480,513,546,580 bps INFO @ Thu, 30 Apr 2015 11:25:46: #2.2 Generate R script for model : SRX080086.05_model.r WARNING @ Thu, 30 Apr 2015 11:25:46: #2 Since the d (43) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 30 Apr 2015 11:25:46: #2 You may need to consider one of the other alternative d(s): 43,77,112,142,174,209,246,279,307,345,373,447,480,513,546,580 WARNING @ Thu, 30 Apr 2015 11:25:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 30 Apr 2015 11:25:46: #3 Call peaks... INFO @ Thu, 30 Apr 2015 11:25:46: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 30 Apr 2015 11:25:47: start X-correlation... INFO @ Thu, 30 Apr 2015 11:25:47: end of X-cor INFO @ Thu, 30 Apr 2015 11:25:47: #2 finished! INFO @ Thu, 30 Apr 2015 11:25:47: #2 predicted fragment length is 43 bps INFO @ Thu, 30 Apr 2015 11:25:47: #2 alternative fragment length(s) may be 43,77,112,142,174,209,246,279,307,345,373,447,480,513,546,580 bps INFO @ Thu, 30 Apr 2015 11:25:47: #2.2 Generate R script for model : SRX080086.10_model.r WARNING @ Thu, 30 Apr 2015 11:25:47: #2 Since the d (43) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 30 Apr 2015 11:25:47: #2 You may need to consider one of the other alternative d(s): 43,77,112,142,174,209,246,279,307,345,373,447,480,513,546,580 WARNING @ Thu, 30 Apr 2015 11:25:47: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 30 Apr 2015 11:25:47: #3 Call peaks... INFO @ Thu, 30 Apr 2015 11:25:47: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 30 Apr 2015 11:25:47: start X-correlation... INFO @ Thu, 30 Apr 2015 11:25:47: end of X-cor INFO @ Thu, 30 Apr 2015 11:25:47: #2 finished! INFO @ Thu, 30 Apr 2015 11:25:47: #2 predicted fragment length is 43 bps INFO @ Thu, 30 Apr 2015 11:25:47: #2 alternative fragment length(s) may be 43,77,112,142,174,209,246,279,307,345,373,447,480,513,546,580 bps INFO @ Thu, 30 Apr 2015 11:25:47: #2.2 Generate R script for model : SRX080086.20_model.r WARNING @ Thu, 30 Apr 2015 11:25:47: #2 Since the d (43) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 30 Apr 2015 11:25:47: #2 You may need to consider one of the other alternative d(s): 43,77,112,142,174,209,246,279,307,345,373,447,480,513,546,580 WARNING @ Thu, 30 Apr 2015 11:25:47: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 30 Apr 2015 11:25:47: #3 Call peaks... INFO @ Thu, 30 Apr 2015 11:25:47: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 30 Apr 2015 11:26:00: #3 Call peaks for each chromosome... INFO @ Thu, 30 Apr 2015 11:26:01: #3 Call peaks for each chromosome... INFO @ Thu, 30 Apr 2015 11:26:02: #3 Call peaks for each chromosome... INFO @ Thu, 30 Apr 2015 11:26:10: #4 Write output xls file... SRX080086.05_peaks.xls INFO @ Thu, 30 Apr 2015 11:26:10: #4 Write peak in narrowPeak format file... SRX080086.05_peaks.narrowPeak INFO @ Thu, 30 Apr 2015 11:26:10: #4 Write summits bed file... SRX080086.05_summits.bed INFO @ Thu, 30 Apr 2015 11:26:10: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (116 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 30 Apr 2015 11:26:11: #4 Write output xls file... SRX080086.20_peaks.xls INFO @ Thu, 30 Apr 2015 11:26:11: #4 Write peak in narrowPeak format file... SRX080086.20_peaks.narrowPeak INFO @ Thu, 30 Apr 2015 11:26:11: #4 Write summits bed file... SRX080086.20_summits.bed INFO @ Thu, 30 Apr 2015 11:26:11: Done! pass1 - making usageList (4 chroms): 1 millis pass2 - checking and writing primary data (6 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Thu, 30 Apr 2015 11:26:14: #4 Write output xls file... SRX080086.10_peaks.xls INFO @ Thu, 30 Apr 2015 11:26:14: #4 Write peak in narrowPeak format file... SRX080086.10_peaks.narrowPeak INFO @ Thu, 30 Apr 2015 11:26:14: #4 Write summits bed file... SRX080086.10_summits.bed INFO @ Thu, 30 Apr 2015 11:26:14: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (29 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。