Job ID = 2237030 sra ファイルのダウンロード中... Completed: 40506K bytes transferred in 4 seconds (78895K bits/sec), in 1 file, 2 directories. % Total % Received % Xferd Average Speed Time Time Time Current Dload Upload Total Spent Left Speed 0 0 0 0 0 0 0 0 --:--:-- --:--:-- --:--:-- 0 100 16380 0 16380 0 0 23965 0 --:--:-- --:--:-- --:--:-- 33225 100 37988 0 37988 0 0 55500 0 --:--:-- --:--:-- --:--:-- 76898 sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 2502361 spots for /home/okishinya/chipatlas/results/ce10/SRX080085/SRR298905.sra Written 2502361 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:21 2502361 reads; of these: 2502361 (100.00%) were unpaired; of these: 973112 (38.89%) aligned 0 times 1299526 (51.93%) aligned exactly 1 time 229723 (9.18%) aligned >1 times 61.11% overall alignment rate Time searching: 00:00:22 Overall time: 00:00:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 728810 / 1529249 = 0.4766 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Thu, 30 Apr 2015 11:24:29: # Command line: callpeak -t SRX080085.bam -f BAM -g ce -n SRX080085.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX080085.10 # format = BAM # ChIP-seq file = ['SRX080085.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Thu, 30 Apr 2015 11:24:29: #1 read tag files... INFO @ Thu, 30 Apr 2015 11:24:29: #1 read treatment tags... INFO @ Thu, 30 Apr 2015 11:24:29: # Command line: callpeak -t SRX080085.bam -f BAM -g ce -n SRX080085.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX080085.05 # format = BAM # ChIP-seq file = ['SRX080085.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Thu, 30 Apr 2015 11:24:29: #1 read tag files... INFO @ Thu, 30 Apr 2015 11:24:29: # Command line: callpeak -t SRX080085.bam -f BAM -g ce -n SRX080085.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX080085.20 # format = BAM # ChIP-seq file = ['SRX080085.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Thu, 30 Apr 2015 11:24:29: #1 read treatment tags... INFO @ Thu, 30 Apr 2015 11:24:29: #1 read tag files... INFO @ Thu, 30 Apr 2015 11:24:29: #1 read treatment tags... INFO @ Thu, 30 Apr 2015 11:24:32: #1 tag size is determined as 32 bps INFO @ Thu, 30 Apr 2015 11:24:32: #1 tag size = 32 INFO @ Thu, 30 Apr 2015 11:24:32: #1 total tags in treatment: 800439 INFO @ Thu, 30 Apr 2015 11:24:32: #1 user defined the maximum tags... INFO @ Thu, 30 Apr 2015 11:24:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 30 Apr 2015 11:24:33: #1 tags after filtering in treatment: 800421 INFO @ Thu, 30 Apr 2015 11:24:33: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 30 Apr 2015 11:24:33: #1 finished! INFO @ Thu, 30 Apr 2015 11:24:33: #2 Build Peak Model... INFO @ Thu, 30 Apr 2015 11:24:33: #1 tag size is determined as 32 bps INFO @ Thu, 30 Apr 2015 11:24:33: #1 tag size = 32 INFO @ Thu, 30 Apr 2015 11:24:33: #1 total tags in treatment: 800439 INFO @ Thu, 30 Apr 2015 11:24:33: #1 user defined the maximum tags... INFO @ Thu, 30 Apr 2015 11:24:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 30 Apr 2015 11:24:33: #1 tag size is determined as 32 bps INFO @ Thu, 30 Apr 2015 11:24:33: #1 tag size = 32 INFO @ Thu, 30 Apr 2015 11:24:33: #1 total tags in treatment: 800439 INFO @ Thu, 30 Apr 2015 11:24:33: #1 user defined the maximum tags... INFO @ Thu, 30 Apr 2015 11:24:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 30 Apr 2015 11:24:33: #1 tags after filtering in treatment: 800421 INFO @ Thu, 30 Apr 2015 11:24:33: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 30 Apr 2015 11:24:33: #1 finished! INFO @ Thu, 30 Apr 2015 11:24:33: #2 Build Peak Model... INFO @ Thu, 30 Apr 2015 11:24:33: #1 tags after filtering in treatment: 800421 INFO @ Thu, 30 Apr 2015 11:24:33: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 30 Apr 2015 11:24:33: #1 finished! INFO @ Thu, 30 Apr 2015 11:24:33: #2 Build Peak Model... INFO @ Thu, 30 Apr 2015 11:24:33: #2 number of paired peaks: 1942 INFO @ Thu, 30 Apr 2015 11:24:33: start model_add_line... INFO @ Thu, 30 Apr 2015 11:24:33: #2 number of paired peaks: 1942 INFO @ Thu, 30 Apr 2015 11:24:33: start model_add_line... INFO @ Thu, 30 Apr 2015 11:24:33: #2 number of paired peaks: 1942 INFO @ Thu, 30 Apr 2015 11:24:33: start model_add_line... INFO @ Thu, 30 Apr 2015 11:24:35: start X-correlation... INFO @ Thu, 30 Apr 2015 11:24:35: end of X-cor INFO @ Thu, 30 Apr 2015 11:24:35: #2 finished! INFO @ Thu, 30 Apr 2015 11:24:35: #2 predicted fragment length is 123 bps INFO @ Thu, 30 Apr 2015 11:24:35: #2 alternative fragment length(s) may be 123 bps INFO @ Thu, 30 Apr 2015 11:24:35: #2.2 Generate R script for model : SRX080085.10_model.r INFO @ Thu, 30 Apr 2015 11:24:35: #3 Call peaks... INFO @ Thu, 30 Apr 2015 11:24:35: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 30 Apr 2015 11:24:35: start X-correlation... INFO @ Thu, 30 Apr 2015 11:24:35: end of X-cor INFO @ Thu, 30 Apr 2015 11:24:35: #2 finished! INFO @ Thu, 30 Apr 2015 11:24:35: #2 predicted fragment length is 123 bps INFO @ Thu, 30 Apr 2015 11:24:35: #2 alternative fragment length(s) may be 123 bps INFO @ Thu, 30 Apr 2015 11:24:35: #2.2 Generate R script for model : SRX080085.05_model.r INFO @ Thu, 30 Apr 2015 11:24:35: #3 Call peaks... INFO @ Thu, 30 Apr 2015 11:24:35: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 30 Apr 2015 11:24:35: start X-correlation... INFO @ Thu, 30 Apr 2015 11:24:35: end of X-cor INFO @ Thu, 30 Apr 2015 11:24:35: #2 finished! INFO @ Thu, 30 Apr 2015 11:24:35: #2 predicted fragment length is 123 bps INFO @ Thu, 30 Apr 2015 11:24:35: #2 alternative fragment length(s) may be 123 bps INFO @ Thu, 30 Apr 2015 11:24:35: #2.2 Generate R script for model : SRX080085.20_model.r INFO @ Thu, 30 Apr 2015 11:24:35: #3 Call peaks... INFO @ Thu, 30 Apr 2015 11:24:35: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 30 Apr 2015 11:24:40: #3 Call peaks for each chromosome... INFO @ Thu, 30 Apr 2015 11:24:40: #3 Call peaks for each chromosome... INFO @ Thu, 30 Apr 2015 11:24:40: #3 Call peaks for each chromosome... INFO @ Thu, 30 Apr 2015 11:24:43: #4 Write output xls file... SRX080085.20_peaks.xls INFO @ Thu, 30 Apr 2015 11:24:43: #4 Write peak in narrowPeak format file... SRX080085.20_peaks.narrowPeak INFO @ Thu, 30 Apr 2015 11:24:43: #4 Write summits bed file... SRX080085.20_summits.bed INFO @ Thu, 30 Apr 2015 11:24:43: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (414 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Thu, 30 Apr 2015 11:24:43: #4 Write output xls file... SRX080085.10_peaks.xls INFO @ Thu, 30 Apr 2015 11:24:43: #4 Write peak in narrowPeak format file... SRX080085.10_peaks.narrowPeak INFO @ Thu, 30 Apr 2015 11:24:43: #4 Write summits bed file... SRX080085.10_summits.bed INFO @ Thu, 30 Apr 2015 11:24:43: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (866 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 30 Apr 2015 11:24:44: #4 Write output xls file... SRX080085.05_peaks.xls INFO @ Thu, 30 Apr 2015 11:24:44: #4 Write peak in narrowPeak format file... SRX080085.05_peaks.narrowPeak INFO @ Thu, 30 Apr 2015 11:24:44: #4 Write summits bed file... SRX080085.05_summits.bed INFO @ Thu, 30 Apr 2015 11:24:44: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1647 records, 4 fields): 3 millis CompletedMACS2peakCalling BigWig に変換しました。