Job ID = 2589293 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 7,263,691 reads read : 7,263,691 reads written : 7,263,691 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR217366.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:44 7263691 reads; of these: 7263691 (100.00%) were unpaired; of these: 4582137 (63.08%) aligned 0 times 2363373 (32.54%) aligned exactly 1 time 318181 (4.38%) aligned >1 times 36.92% overall alignment rate Time searching: 00:00:44 Overall time: 00:00:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 325687 / 2681554 = 0.1215 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 17:21:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:21:12: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:21:12: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:21:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:21:13: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:21:13: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:21:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:21:14: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:21:14: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:21:20: 1000000 INFO @ Mon, 12 Aug 2019 17:21:22: 1000000 INFO @ Mon, 12 Aug 2019 17:21:22: 1000000 INFO @ Mon, 12 Aug 2019 17:21:29: 2000000 INFO @ Mon, 12 Aug 2019 17:21:30: 2000000 INFO @ Mon, 12 Aug 2019 17:21:30: 2000000 INFO @ Mon, 12 Aug 2019 17:21:32: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:21:32: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:21:32: #1 total tags in treatment: 2355867 INFO @ Mon, 12 Aug 2019 17:21:32: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:21:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:21:32: #1 tags after filtering in treatment: 2355867 INFO @ Mon, 12 Aug 2019 17:21:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:21:32: #1 finished! INFO @ Mon, 12 Aug 2019 17:21:32: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:21:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:21:33: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:21:33: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:21:33: #1 total tags in treatment: 2355867 INFO @ Mon, 12 Aug 2019 17:21:33: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:21:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:21:33: #2 number of paired peaks: 3037 INFO @ Mon, 12 Aug 2019 17:21:33: start model_add_line... INFO @ Mon, 12 Aug 2019 17:21:33: #1 tags after filtering in treatment: 2355867 INFO @ Mon, 12 Aug 2019 17:21:33: start X-correlation... INFO @ Mon, 12 Aug 2019 17:21:33: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:21:33: #1 finished! INFO @ Mon, 12 Aug 2019 17:21:33: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:21:33: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:21:33: end of X-cor INFO @ Mon, 12 Aug 2019 17:21:33: #2 finished! INFO @ Mon, 12 Aug 2019 17:21:33: #2 predicted fragment length is 160 bps INFO @ Mon, 12 Aug 2019 17:21:33: #2 alternative fragment length(s) may be 160 bps INFO @ Mon, 12 Aug 2019 17:21:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.05_model.r INFO @ Mon, 12 Aug 2019 17:21:33: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:21:33: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:21:33: #2 number of paired peaks: 3037 INFO @ Mon, 12 Aug 2019 17:21:33: start model_add_line... INFO @ Mon, 12 Aug 2019 17:21:33: start X-correlation... INFO @ Mon, 12 Aug 2019 17:21:33: end of X-cor INFO @ Mon, 12 Aug 2019 17:21:33: #2 finished! INFO @ Mon, 12 Aug 2019 17:21:33: #2 predicted fragment length is 160 bps INFO @ Mon, 12 Aug 2019 17:21:33: #2 alternative fragment length(s) may be 160 bps INFO @ Mon, 12 Aug 2019 17:21:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.10_model.r INFO @ Mon, 12 Aug 2019 17:21:33: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:21:33: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:21:33: #1 tag size is determined as 32 bps INFO @ Mon, 12 Aug 2019 17:21:33: #1 tag size = 32 INFO @ Mon, 12 Aug 2019 17:21:33: #1 total tags in treatment: 2355867 INFO @ Mon, 12 Aug 2019 17:21:33: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:21:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:21:33: #1 tags after filtering in treatment: 2355867 INFO @ Mon, 12 Aug 2019 17:21:33: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:21:33: #1 finished! INFO @ Mon, 12 Aug 2019 17:21:33: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:21:33: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:21:34: #2 number of paired peaks: 3037 INFO @ Mon, 12 Aug 2019 17:21:34: start model_add_line... INFO @ Mon, 12 Aug 2019 17:21:34: start X-correlation... INFO @ Mon, 12 Aug 2019 17:21:34: end of X-cor INFO @ Mon, 12 Aug 2019 17:21:34: #2 finished! INFO @ Mon, 12 Aug 2019 17:21:34: #2 predicted fragment length is 160 bps INFO @ Mon, 12 Aug 2019 17:21:34: #2 alternative fragment length(s) may be 160 bps INFO @ Mon, 12 Aug 2019 17:21:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.20_model.r INFO @ Mon, 12 Aug 2019 17:21:34: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:21:34: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:21:41: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:21:41: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:21:42: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:21:45: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.10_peaks.xls INFO @ Mon, 12 Aug 2019 17:21:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:21:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.10_summits.bed INFO @ Mon, 12 Aug 2019 17:21:45: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (4289 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:21:45: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.05_peaks.xls INFO @ Mon, 12 Aug 2019 17:21:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:21:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.05_summits.bed INFO @ Mon, 12 Aug 2019 17:21:45: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (7051 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:21:46: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.20_peaks.xls INFO @ Mon, 12 Aug 2019 17:21:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:21:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX065660/SRX065660.20_summits.bed INFO @ Mon, 12 Aug 2019 17:21:46: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1788 records, 4 fields): 5 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。