Job ID = 9157353 sra ファイルのダウンロード中... Completed: 697135K bytes transferred in 9 seconds (589268K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 30768648 spots for /home/okishinya/chipatlas/results/ce10/SRX060162/SRR192328.sra Written 30768648 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:09 30768648 reads; of these: 30768648 (100.00%) were unpaired; of these: 898686 (2.92%) aligned 0 times 24718036 (80.34%) aligned exactly 1 time 5151926 (16.74%) aligned >1 times 97.08% overall alignment rate Time searching: 00:06:09 Overall time: 00:06:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3756432 / 29869962 = 0.1258 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 11:40:12: # Command line: callpeak -t SRX060162.bam -f BAM -g ce -n SRX060162.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX060162.20 # format = BAM # ChIP-seq file = ['SRX060162.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:40:12: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:40:12: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:40:12: # Command line: callpeak -t SRX060162.bam -f BAM -g ce -n SRX060162.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX060162.05 # format = BAM # ChIP-seq file = ['SRX060162.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:40:12: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:40:12: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:40:12: # Command line: callpeak -t SRX060162.bam -f BAM -g ce -n SRX060162.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX060162.10 # format = BAM # ChIP-seq file = ['SRX060162.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 11:40:12: #1 read tag files... INFO @ Tue, 27 Jun 2017 11:40:12: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 11:40:19: 1000000 INFO @ Tue, 27 Jun 2017 11:40:19: 1000000 INFO @ Tue, 27 Jun 2017 11:40:19: 1000000 INFO @ Tue, 27 Jun 2017 11:40:25: 2000000 INFO @ Tue, 27 Jun 2017 11:40:26: 2000000 INFO @ Tue, 27 Jun 2017 11:40:26: 2000000 INFO @ Tue, 27 Jun 2017 11:40:32: 3000000 INFO @ Tue, 27 Jun 2017 11:40:33: 3000000 INFO @ Tue, 27 Jun 2017 11:40:33: 3000000 INFO @ Tue, 27 Jun 2017 11:40:38: 4000000 INFO @ Tue, 27 Jun 2017 11:40:40: 4000000 INFO @ Tue, 27 Jun 2017 11:40:40: 4000000 INFO @ Tue, 27 Jun 2017 11:40:45: 5000000 INFO @ Tue, 27 Jun 2017 11:40:47: 5000000 INFO @ Tue, 27 Jun 2017 11:40:47: 5000000 INFO @ Tue, 27 Jun 2017 11:40:51: 6000000 INFO @ Tue, 27 Jun 2017 11:40:54: 6000000 INFO @ Tue, 27 Jun 2017 11:40:54: 6000000 INFO @ Tue, 27 Jun 2017 11:40:58: 7000000 INFO @ Tue, 27 Jun 2017 11:41:01: 7000000 INFO @ Tue, 27 Jun 2017 11:41:01: 7000000 INFO @ Tue, 27 Jun 2017 11:41:05: 8000000 INFO @ Tue, 27 Jun 2017 11:41:07: 8000000 INFO @ Tue, 27 Jun 2017 11:41:08: 8000000 INFO @ Tue, 27 Jun 2017 11:41:11: 9000000 INFO @ Tue, 27 Jun 2017 11:41:14: 9000000 INFO @ Tue, 27 Jun 2017 11:41:14: 9000000 INFO @ Tue, 27 Jun 2017 11:41:18: 10000000 INFO @ Tue, 27 Jun 2017 11:41:21: 10000000 INFO @ Tue, 27 Jun 2017 11:41:21: 10000000 INFO @ Tue, 27 Jun 2017 11:41:24: 11000000 INFO @ Tue, 27 Jun 2017 11:41:28: 11000000 INFO @ Tue, 27 Jun 2017 11:41:28: 11000000 INFO @ Tue, 27 Jun 2017 11:41:31: 12000000 INFO @ Tue, 27 Jun 2017 11:41:35: 12000000 INFO @ Tue, 27 Jun 2017 11:41:35: 12000000 INFO @ Tue, 27 Jun 2017 11:41:37: 13000000 INFO @ Tue, 27 Jun 2017 11:41:42: 13000000 INFO @ Tue, 27 Jun 2017 11:41:42: 13000000 INFO @ Tue, 27 Jun 2017 11:41:43: 14000000 INFO @ Tue, 27 Jun 2017 11:41:49: 14000000 INFO @ Tue, 27 Jun 2017 11:41:49: 14000000 INFO @ Tue, 27 Jun 2017 11:41:50: 15000000 INFO @ Tue, 27 Jun 2017 11:41:56: 15000000 INFO @ Tue, 27 Jun 2017 11:41:56: 15000000 INFO @ Tue, 27 Jun 2017 11:41:56: 16000000 INFO @ Tue, 27 Jun 2017 11:42:03: 17000000 INFO @ Tue, 27 Jun 2017 11:42:03: 16000000 INFO @ Tue, 27 Jun 2017 11:42:03: 16000000 INFO @ Tue, 27 Jun 2017 11:42:09: 18000000 INFO @ Tue, 27 Jun 2017 11:42:10: 17000000 INFO @ Tue, 27 Jun 2017 11:42:10: 17000000 INFO @ Tue, 27 Jun 2017 11:42:15: 19000000 INFO @ Tue, 27 Jun 2017 11:42:17: 18000000 INFO @ Tue, 27 Jun 2017 11:42:17: 18000000 INFO @ Tue, 27 Jun 2017 11:42:21: 20000000 INFO @ Tue, 27 Jun 2017 11:42:24: 19000000 INFO @ Tue, 27 Jun 2017 11:42:24: 19000000 INFO @ Tue, 27 Jun 2017 11:42:28: 21000000 INFO @ Tue, 27 Jun 2017 11:42:31: 20000000 INFO @ Tue, 27 Jun 2017 11:42:31: 20000000 INFO @ Tue, 27 Jun 2017 11:42:34: 22000000 INFO @ Tue, 27 Jun 2017 11:42:38: 21000000 INFO @ Tue, 27 Jun 2017 11:42:38: 21000000 INFO @ Tue, 27 Jun 2017 11:42:40: 23000000 INFO @ Tue, 27 Jun 2017 11:42:45: 22000000 INFO @ Tue, 27 Jun 2017 11:42:46: 22000000 INFO @ Tue, 27 Jun 2017 11:42:46: 24000000 INFO @ Tue, 27 Jun 2017 11:42:52: 23000000 INFO @ Tue, 27 Jun 2017 11:42:53: 25000000 INFO @ Tue, 27 Jun 2017 11:42:53: 23000000 INFO @ Tue, 27 Jun 2017 11:42:59: 26000000 INFO @ Tue, 27 Jun 2017 11:42:59: 24000000 INFO @ Tue, 27 Jun 2017 11:43:00: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 11:43:00: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 11:43:00: #1 total tags in treatment: 26113530 INFO @ Tue, 27 Jun 2017 11:43:00: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:43:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:43:00: 24000000 INFO @ Tue, 27 Jun 2017 11:43:00: #1 tags after filtering in treatment: 26113530 INFO @ Tue, 27 Jun 2017 11:43:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:43:00: #1 finished! INFO @ Tue, 27 Jun 2017 11:43:00: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:43:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:43:02: #2 number of paired peaks: 96 WARNING @ Tue, 27 Jun 2017 11:43:02: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:43:02: Process for pairing-model is terminated! cat: SRX060162.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX060162.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX060162.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX060162.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 11:43:06: 25000000 INFO @ Tue, 27 Jun 2017 11:43:07: 25000000 INFO @ Tue, 27 Jun 2017 11:43:13: 26000000 INFO @ Tue, 27 Jun 2017 11:43:14: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 11:43:14: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 11:43:14: #1 total tags in treatment: 26113530 INFO @ Tue, 27 Jun 2017 11:43:14: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:43:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:43:14: 26000000 INFO @ Tue, 27 Jun 2017 11:43:15: #1 tags after filtering in treatment: 26113530 INFO @ Tue, 27 Jun 2017 11:43:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:43:15: #1 finished! INFO @ Tue, 27 Jun 2017 11:43:15: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:43:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:43:15: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 11:43:15: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 11:43:15: #1 total tags in treatment: 26113530 INFO @ Tue, 27 Jun 2017 11:43:15: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 11:43:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 11:43:16: #1 tags after filtering in treatment: 26113530 INFO @ Tue, 27 Jun 2017 11:43:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 11:43:16: #1 finished! INFO @ Tue, 27 Jun 2017 11:43:16: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 11:43:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 11:43:16: #2 number of paired peaks: 96 WARNING @ Tue, 27 Jun 2017 11:43:16: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:43:16: Process for pairing-model is terminated! cat: SRX060162.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX060162.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX060162.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX060162.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 11:43:17: #2 number of paired peaks: 96 WARNING @ Tue, 27 Jun 2017 11:43:17: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 11:43:17: Process for pairing-model is terminated! cat: SRX060162.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX060162.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX060162.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX060162.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。