Job ID = 2236856 sra ファイルのダウンロード中... Completed: 58313K bytes transferred in 5 seconds (92328K bits/sec), in 1 file, 2 directories. % Total % Received % Xferd Average Speed Time Time Time Current Dload Upload Total Spent Left Speed 0 0 0 0 0 0 0 0 --:--:-- --:--:-- --:--:-- 0 100 34605 0 34605 0 0 47586 0 --:--:-- --:--:-- --:--:-- 64561 sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 2456763 spots for /home/okishinya/chipatlas/results/ce10/SRX059232/SRR190672.sra Written 2456763 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:27 2456763 reads; of these: 2456763 (100.00%) were unpaired; of these: 464745 (18.92%) aligned 0 times 1568420 (63.84%) aligned exactly 1 time 423598 (17.24%) aligned >1 times 81.08% overall alignment rate Time searching: 00:00:27 Overall time: 00:00:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 307157 / 1992018 = 0.1542 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Thu, 30 Apr 2015 11:11:05: # Command line: callpeak -t SRX059232.bam -f BAM -g ce -n SRX059232.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX059232.05 # format = BAM # ChIP-seq file = ['SRX059232.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Thu, 30 Apr 2015 11:11:05: # Command line: callpeak -t SRX059232.bam -f BAM -g ce -n SRX059232.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX059232.10 # format = BAM # ChIP-seq file = ['SRX059232.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Thu, 30 Apr 2015 11:11:05: #1 read tag files... INFO @ Thu, 30 Apr 2015 11:11:05: #1 read tag files... INFO @ Thu, 30 Apr 2015 11:11:05: #1 read treatment tags... INFO @ Thu, 30 Apr 2015 11:11:05: # Command line: callpeak -t SRX059232.bam -f BAM -g ce -n SRX059232.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX059232.20 # format = BAM # ChIP-seq file = ['SRX059232.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Thu, 30 Apr 2015 11:11:05: #1 read treatment tags... INFO @ Thu, 30 Apr 2015 11:11:05: #1 read tag files... INFO @ Thu, 30 Apr 2015 11:11:05: #1 read treatment tags... INFO @ Thu, 30 Apr 2015 11:11:11: 1000000 INFO @ Thu, 30 Apr 2015 11:11:11: 1000000 INFO @ Thu, 30 Apr 2015 11:11:11: 1000000 INFO @ Thu, 30 Apr 2015 11:11:15: #1 tag size is determined as 36 bps INFO @ Thu, 30 Apr 2015 11:11:15: #1 tag size = 36 INFO @ Thu, 30 Apr 2015 11:11:15: #1 total tags in treatment: 1684861 INFO @ Thu, 30 Apr 2015 11:11:15: #1 user defined the maximum tags... INFO @ Thu, 30 Apr 2015 11:11:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 30 Apr 2015 11:11:15: #1 tags after filtering in treatment: 1684720 INFO @ Thu, 30 Apr 2015 11:11:15: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 30 Apr 2015 11:11:15: #1 finished! INFO @ Thu, 30 Apr 2015 11:11:15: #2 Build Peak Model... INFO @ Thu, 30 Apr 2015 11:11:16: #1 tag size is determined as 36 bps INFO @ Thu, 30 Apr 2015 11:11:16: #1 tag size = 36 INFO @ Thu, 30 Apr 2015 11:11:16: #1 total tags in treatment: 1684861 INFO @ Thu, 30 Apr 2015 11:11:16: #1 user defined the maximum tags... INFO @ Thu, 30 Apr 2015 11:11:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 30 Apr 2015 11:11:16: #1 tag size is determined as 36 bps INFO @ Thu, 30 Apr 2015 11:11:16: #1 tag size = 36 INFO @ Thu, 30 Apr 2015 11:11:16: #1 total tags in treatment: 1684861 INFO @ Thu, 30 Apr 2015 11:11:16: #1 user defined the maximum tags... INFO @ Thu, 30 Apr 2015 11:11:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 30 Apr 2015 11:11:16: #2 number of paired peaks: 1307 INFO @ Thu, 30 Apr 2015 11:11:16: start model_add_line... INFO @ Thu, 30 Apr 2015 11:11:16: #1 tags after filtering in treatment: 1684720 INFO @ Thu, 30 Apr 2015 11:11:16: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 30 Apr 2015 11:11:16: #1 finished! INFO @ Thu, 30 Apr 2015 11:11:16: #2 Build Peak Model... INFO @ Thu, 30 Apr 2015 11:11:16: #1 tags after filtering in treatment: 1684720 INFO @ Thu, 30 Apr 2015 11:11:16: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 30 Apr 2015 11:11:16: #1 finished! INFO @ Thu, 30 Apr 2015 11:11:16: #2 Build Peak Model... INFO @ Thu, 30 Apr 2015 11:11:16: #2 number of paired peaks: 1307 INFO @ Thu, 30 Apr 2015 11:11:16: start model_add_line... INFO @ Thu, 30 Apr 2015 11:11:16: #2 number of paired peaks: 1307 INFO @ Thu, 30 Apr 2015 11:11:16: start model_add_line... INFO @ Thu, 30 Apr 2015 11:11:18: start X-correlation... INFO @ Thu, 30 Apr 2015 11:11:18: end of X-cor INFO @ Thu, 30 Apr 2015 11:11:18: #2 finished! INFO @ Thu, 30 Apr 2015 11:11:18: #2 predicted fragment length is 110 bps INFO @ Thu, 30 Apr 2015 11:11:18: #2 alternative fragment length(s) may be 110 bps INFO @ Thu, 30 Apr 2015 11:11:18: #2.2 Generate R script for model : SRX059232.20_model.r INFO @ Thu, 30 Apr 2015 11:11:18: #3 Call peaks... INFO @ Thu, 30 Apr 2015 11:11:18: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 30 Apr 2015 11:11:19: start X-correlation... INFO @ Thu, 30 Apr 2015 11:11:19: end of X-cor INFO @ Thu, 30 Apr 2015 11:11:19: #2 finished! INFO @ Thu, 30 Apr 2015 11:11:19: #2 predicted fragment length is 110 bps INFO @ Thu, 30 Apr 2015 11:11:19: #2 alternative fragment length(s) may be 110 bps INFO @ Thu, 30 Apr 2015 11:11:19: #2.2 Generate R script for model : SRX059232.10_model.r INFO @ Thu, 30 Apr 2015 11:11:19: #3 Call peaks... INFO @ Thu, 30 Apr 2015 11:11:19: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 30 Apr 2015 11:11:19: start X-correlation... INFO @ Thu, 30 Apr 2015 11:11:19: end of X-cor INFO @ Thu, 30 Apr 2015 11:11:19: #2 finished! INFO @ Thu, 30 Apr 2015 11:11:19: #2 predicted fragment length is 110 bps INFO @ Thu, 30 Apr 2015 11:11:19: #2 alternative fragment length(s) may be 110 bps INFO @ Thu, 30 Apr 2015 11:11:19: #2.2 Generate R script for model : SRX059232.05_model.r INFO @ Thu, 30 Apr 2015 11:11:19: #3 Call peaks... INFO @ Thu, 30 Apr 2015 11:11:19: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 30 Apr 2015 11:11:29: #3 Call peaks for each chromosome... INFO @ Thu, 30 Apr 2015 11:11:30: #3 Call peaks for each chromosome... INFO @ Thu, 30 Apr 2015 11:11:30: #3 Call peaks for each chromosome... INFO @ Thu, 30 Apr 2015 11:11:37: #4 Write output xls file... SRX059232.20_peaks.xls INFO @ Thu, 30 Apr 2015 11:11:37: #4 Write peak in narrowPeak format file... SRX059232.20_peaks.narrowPeak INFO @ Thu, 30 Apr 2015 11:11:37: #4 Write summits bed file... SRX059232.20_summits.bed INFO @ Thu, 30 Apr 2015 11:11:37: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (837 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 30 Apr 2015 11:11:38: #4 Write output xls file... SRX059232.10_peaks.xls INFO @ Thu, 30 Apr 2015 11:11:38: #4 Write peak in narrowPeak format file... SRX059232.10_peaks.narrowPeak INFO @ Thu, 30 Apr 2015 11:11:38: #4 Write summits bed file... SRX059232.10_summits.bed INFO @ Thu, 30 Apr 2015 11:11:38: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (1230 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Thu, 30 Apr 2015 11:11:39: #4 Write output xls file... SRX059232.05_peaks.xls INFO @ Thu, 30 Apr 2015 11:11:39: #4 Write peak in narrowPeak format file... SRX059232.05_peaks.narrowPeak INFO @ Thu, 30 Apr 2015 11:11:39: #4 Write summits bed file... SRX059232.05_summits.bed INFO @ Thu, 30 Apr 2015 11:11:39: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (1881 records, 4 fields): 4 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。