Job ID = 2589161 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 3,528,845 reads read : 3,528,845 reads written : 3,528,845 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR107544.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:40 3528845 reads; of these: 3528845 (100.00%) were unpaired; of these: 1133565 (32.12%) aligned 0 times 2066174 (58.55%) aligned exactly 1 time 329106 (9.33%) aligned >1 times 67.88% overall alignment rate Time searching: 00:00:40 Overall time: 00:00:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 138325 / 2395280 = 0.0577 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 17:03:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:03:14: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:03:14: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:03:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:03:15: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:03:15: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:03:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:03:16: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:03:16: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:03:22: 1000000 INFO @ Mon, 12 Aug 2019 17:03:23: 1000000 INFO @ Mon, 12 Aug 2019 17:03:25: 1000000 INFO @ Mon, 12 Aug 2019 17:03:29: 2000000 INFO @ Mon, 12 Aug 2019 17:03:30: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:03:30: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:03:30: #1 total tags in treatment: 2256955 INFO @ Mon, 12 Aug 2019 17:03:30: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:03:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:03:31: #1 tags after filtering in treatment: 2256955 INFO @ Mon, 12 Aug 2019 17:03:31: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:03:31: #1 finished! INFO @ Mon, 12 Aug 2019 17:03:31: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:03:31: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:03:31: #2 number of paired peaks: 270 WARNING @ Mon, 12 Aug 2019 17:03:31: Fewer paired peaks (270) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 270 pairs to build model! INFO @ Mon, 12 Aug 2019 17:03:31: start model_add_line... INFO @ Mon, 12 Aug 2019 17:03:31: start X-correlation... INFO @ Mon, 12 Aug 2019 17:03:31: end of X-cor INFO @ Mon, 12 Aug 2019 17:03:31: #2 finished! INFO @ Mon, 12 Aug 2019 17:03:31: #2 predicted fragment length is 38 bps INFO @ Mon, 12 Aug 2019 17:03:31: #2 alternative fragment length(s) may be 2,38,514,584 bps INFO @ Mon, 12 Aug 2019 17:03:31: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.10_model.r WARNING @ Mon, 12 Aug 2019 17:03:31: #2 Since the d (38) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:03:31: #2 You may need to consider one of the other alternative d(s): 2,38,514,584 WARNING @ Mon, 12 Aug 2019 17:03:31: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:03:31: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:03:31: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:03:33: 2000000 INFO @ Mon, 12 Aug 2019 17:03:35: 2000000 INFO @ Mon, 12 Aug 2019 17:03:35: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:03:35: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:03:35: #1 total tags in treatment: 2256955 INFO @ Mon, 12 Aug 2019 17:03:35: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:03:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:03:35: #1 tags after filtering in treatment: 2256955 INFO @ Mon, 12 Aug 2019 17:03:35: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:03:35: #1 finished! INFO @ Mon, 12 Aug 2019 17:03:35: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:03:35: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:03:35: #2 number of paired peaks: 270 WARNING @ Mon, 12 Aug 2019 17:03:35: Fewer paired peaks (270) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 270 pairs to build model! INFO @ Mon, 12 Aug 2019 17:03:35: start model_add_line... INFO @ Mon, 12 Aug 2019 17:03:35: start X-correlation... INFO @ Mon, 12 Aug 2019 17:03:35: end of X-cor INFO @ Mon, 12 Aug 2019 17:03:35: #2 finished! INFO @ Mon, 12 Aug 2019 17:03:35: #2 predicted fragment length is 38 bps INFO @ Mon, 12 Aug 2019 17:03:35: #2 alternative fragment length(s) may be 2,38,514,584 bps INFO @ Mon, 12 Aug 2019 17:03:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.05_model.r WARNING @ Mon, 12 Aug 2019 17:03:35: #2 Since the d (38) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:03:35: #2 You may need to consider one of the other alternative d(s): 2,38,514,584 WARNING @ Mon, 12 Aug 2019 17:03:35: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:03:35: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:03:35: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:03:37: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:03:37: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:03:37: #1 total tags in treatment: 2256955 INFO @ Mon, 12 Aug 2019 17:03:37: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:03:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:03:37: #1 tags after filtering in treatment: 2256955 INFO @ Mon, 12 Aug 2019 17:03:37: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:03:37: #1 finished! INFO @ Mon, 12 Aug 2019 17:03:37: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:03:37: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:03:37: #2 number of paired peaks: 270 WARNING @ Mon, 12 Aug 2019 17:03:37: Fewer paired peaks (270) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 270 pairs to build model! INFO @ Mon, 12 Aug 2019 17:03:37: start model_add_line... INFO @ Mon, 12 Aug 2019 17:03:37: start X-correlation... INFO @ Mon, 12 Aug 2019 17:03:37: end of X-cor INFO @ Mon, 12 Aug 2019 17:03:37: #2 finished! INFO @ Mon, 12 Aug 2019 17:03:37: #2 predicted fragment length is 38 bps INFO @ Mon, 12 Aug 2019 17:03:37: #2 alternative fragment length(s) may be 2,38,514,584 bps INFO @ Mon, 12 Aug 2019 17:03:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.20_model.r WARNING @ Mon, 12 Aug 2019 17:03:37: #2 Since the d (38) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:03:37: #2 You may need to consider one of the other alternative d(s): 2,38,514,584 WARNING @ Mon, 12 Aug 2019 17:03:37: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:03:37: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:03:37: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:03:38: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:03:41: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.10_peaks.xls INFO @ Mon, 12 Aug 2019 17:03:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:03:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.10_summits.bed INFO @ Mon, 12 Aug 2019 17:03:41: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (81 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:03:42: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:03:44: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:03:45: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.05_peaks.xls INFO @ Mon, 12 Aug 2019 17:03:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:03:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.05_summits.bed INFO @ Mon, 12 Aug 2019 17:03:45: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (221 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:03:47: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.20_peaks.xls INFO @ Mon, 12 Aug 2019 17:03:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:03:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX043969/SRX043969.20_summits.bed INFO @ Mon, 12 Aug 2019 17:03:47: Done! pass1 - making usageList (5 chroms): 1 millis pass2 - checking and writing primary data (12 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。