Job ID = 2589141 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 2,643,487 reads read : 2,643,487 reads written : 2,643,487 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR107332.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:32 2643487 reads; of these: 2643487 (100.00%) were unpaired; of these: 473819 (17.92%) aligned 0 times 1879912 (71.11%) aligned exactly 1 time 289756 (10.96%) aligned >1 times 82.08% overall alignment rate Time searching: 00:00:32 Overall time: 00:00:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 102409 / 2169668 = 0.0472 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 17:00:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:00:15: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:00:15: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:00:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:00:16: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:00:16: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:00:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:00:17: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:00:17: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:00:23: 1000000 INFO @ Mon, 12 Aug 2019 17:00:24: 1000000 INFO @ Mon, 12 Aug 2019 17:00:26: 1000000 INFO @ Mon, 12 Aug 2019 17:00:32: 2000000 INFO @ Mon, 12 Aug 2019 17:00:32: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:00:32: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:00:32: #1 total tags in treatment: 2067259 INFO @ Mon, 12 Aug 2019 17:00:32: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:00:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:00:32: #1 tags after filtering in treatment: 2067259 INFO @ Mon, 12 Aug 2019 17:00:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:00:32: #1 finished! INFO @ Mon, 12 Aug 2019 17:00:32: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:00:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:00:32: #2 number of paired peaks: 338 WARNING @ Mon, 12 Aug 2019 17:00:32: Fewer paired peaks (338) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 338 pairs to build model! INFO @ Mon, 12 Aug 2019 17:00:32: start model_add_line... INFO @ Mon, 12 Aug 2019 17:00:32: start X-correlation... INFO @ Mon, 12 Aug 2019 17:00:32: end of X-cor INFO @ Mon, 12 Aug 2019 17:00:32: #2 finished! INFO @ Mon, 12 Aug 2019 17:00:32: #2 predicted fragment length is 44 bps INFO @ Mon, 12 Aug 2019 17:00:32: #2 alternative fragment length(s) may be 44,66,550 bps INFO @ Mon, 12 Aug 2019 17:00:32: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.05_model.r WARNING @ Mon, 12 Aug 2019 17:00:32: #2 Since the d (44) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:00:32: #2 You may need to consider one of the other alternative d(s): 44,66,550 WARNING @ Mon, 12 Aug 2019 17:00:32: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:00:32: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:00:32: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:00:35: 2000000 INFO @ Mon, 12 Aug 2019 17:00:35: 2000000 INFO @ Mon, 12 Aug 2019 17:00:36: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:00:36: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:00:36: #1 total tags in treatment: 2067259 INFO @ Mon, 12 Aug 2019 17:00:36: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:00:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:00:36: #1 tags after filtering in treatment: 2067259 INFO @ Mon, 12 Aug 2019 17:00:36: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:00:36: #1 finished! INFO @ Mon, 12 Aug 2019 17:00:36: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:00:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:00:36: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:00:36: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:00:36: #1 total tags in treatment: 2067259 INFO @ Mon, 12 Aug 2019 17:00:36: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:00:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:00:36: #1 tags after filtering in treatment: 2067259 INFO @ Mon, 12 Aug 2019 17:00:36: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:00:36: #1 finished! INFO @ Mon, 12 Aug 2019 17:00:36: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:00:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:00:36: #2 number of paired peaks: 338 WARNING @ Mon, 12 Aug 2019 17:00:36: Fewer paired peaks (338) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 338 pairs to build model! INFO @ Mon, 12 Aug 2019 17:00:36: start model_add_line... INFO @ Mon, 12 Aug 2019 17:00:36: start X-correlation... INFO @ Mon, 12 Aug 2019 17:00:36: end of X-cor INFO @ Mon, 12 Aug 2019 17:00:36: #2 finished! INFO @ Mon, 12 Aug 2019 17:00:36: #2 predicted fragment length is 44 bps INFO @ Mon, 12 Aug 2019 17:00:36: #2 alternative fragment length(s) may be 44,66,550 bps INFO @ Mon, 12 Aug 2019 17:00:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.20_model.r WARNING @ Mon, 12 Aug 2019 17:00:36: #2 Since the d (44) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:00:36: #2 You may need to consider one of the other alternative d(s): 44,66,550 WARNING @ Mon, 12 Aug 2019 17:00:36: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:00:36: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:00:36: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:00:36: #2 number of paired peaks: 338 WARNING @ Mon, 12 Aug 2019 17:00:36: Fewer paired peaks (338) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 338 pairs to build model! INFO @ Mon, 12 Aug 2019 17:00:36: start model_add_line... INFO @ Mon, 12 Aug 2019 17:00:36: start X-correlation... INFO @ Mon, 12 Aug 2019 17:00:36: end of X-cor INFO @ Mon, 12 Aug 2019 17:00:36: #2 finished! INFO @ Mon, 12 Aug 2019 17:00:36: #2 predicted fragment length is 44 bps INFO @ Mon, 12 Aug 2019 17:00:36: #2 alternative fragment length(s) may be 44,66,550 bps INFO @ Mon, 12 Aug 2019 17:00:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.10_model.r WARNING @ Mon, 12 Aug 2019 17:00:36: #2 Since the d (44) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 12 Aug 2019 17:00:36: #2 You may need to consider one of the other alternative d(s): 44,66,550 WARNING @ Mon, 12 Aug 2019 17:00:36: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 12 Aug 2019 17:00:36: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:00:36: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:00:39: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:00:42: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.05_peaks.xls INFO @ Mon, 12 Aug 2019 17:00:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:00:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.05_summits.bed INFO @ Mon, 12 Aug 2019 17:00:42: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (168 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:00:42: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:00:42: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:00:45: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.20_peaks.xls INFO @ Mon, 12 Aug 2019 17:00:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:00:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.20_summits.bed INFO @ Mon, 12 Aug 2019 17:00:45: Done! INFO @ Mon, 12 Aug 2019 17:00:46: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.10_peaks.xls INFO @ Mon, 12 Aug 2019 17:00:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:00:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX043870/SRX043870.10_summits.bed INFO @ Mon, 12 Aug 2019 17:00:46: Done! pass1 - making usageList (5 chroms): 1 millis pass2 - checking and writing primary data (6 records, 4 fields): 2 millis CompletedMACS2peakCalling pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (43 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。