Job ID = 2589133 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 6,191,439 reads read : 6,191,439 reads written : 6,191,439 spots read : 5,071,217 reads read : 5,071,217 reads written : 5,071,217 rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR107322.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR107323.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:07 11262656 reads; of these: 11262656 (100.00%) were unpaired; of these: 9330151 (82.84%) aligned 0 times 1695017 (15.05%) aligned exactly 1 time 237488 (2.11%) aligned >1 times 17.16% overall alignment rate Time searching: 00:01:07 Overall time: 00:01:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 173769 / 1932505 = 0.0899 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 17:00:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:00:49: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:00:49: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:00:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:00:50: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:00:50: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:00:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 17:00:51: #1 read tag files... INFO @ Mon, 12 Aug 2019 17:00:51: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 17:00:57: 1000000 INFO @ Mon, 12 Aug 2019 17:00:59: 1000000 INFO @ Mon, 12 Aug 2019 17:01:00: 1000000 INFO @ Mon, 12 Aug 2019 17:01:03: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:01:03: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:01:03: #1 total tags in treatment: 1758736 INFO @ Mon, 12 Aug 2019 17:01:03: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:01:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:01:03: #1 tags after filtering in treatment: 1758736 INFO @ Mon, 12 Aug 2019 17:01:03: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:01:03: #1 finished! INFO @ Mon, 12 Aug 2019 17:01:03: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:01:03: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:01:03: #2 number of paired peaks: 584 WARNING @ Mon, 12 Aug 2019 17:01:03: Fewer paired peaks (584) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 584 pairs to build model! INFO @ Mon, 12 Aug 2019 17:01:03: start model_add_line... INFO @ Mon, 12 Aug 2019 17:01:03: start X-correlation... INFO @ Mon, 12 Aug 2019 17:01:03: end of X-cor INFO @ Mon, 12 Aug 2019 17:01:03: #2 finished! INFO @ Mon, 12 Aug 2019 17:01:03: #2 predicted fragment length is 146 bps INFO @ Mon, 12 Aug 2019 17:01:03: #2 alternative fragment length(s) may be 146,582 bps INFO @ Mon, 12 Aug 2019 17:01:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.05_model.r INFO @ Mon, 12 Aug 2019 17:01:03: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:01:03: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:01:06: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:01:06: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:01:06: #1 total tags in treatment: 1758736 INFO @ Mon, 12 Aug 2019 17:01:06: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:01:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:01:06: #1 tags after filtering in treatment: 1758736 INFO @ Mon, 12 Aug 2019 17:01:06: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:01:06: #1 finished! INFO @ Mon, 12 Aug 2019 17:01:06: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:01:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:01:06: #2 number of paired peaks: 584 WARNING @ Mon, 12 Aug 2019 17:01:06: Fewer paired peaks (584) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 584 pairs to build model! INFO @ Mon, 12 Aug 2019 17:01:06: start model_add_line... INFO @ Mon, 12 Aug 2019 17:01:06: start X-correlation... INFO @ Mon, 12 Aug 2019 17:01:06: end of X-cor INFO @ Mon, 12 Aug 2019 17:01:06: #2 finished! INFO @ Mon, 12 Aug 2019 17:01:06: #2 predicted fragment length is 146 bps INFO @ Mon, 12 Aug 2019 17:01:06: #2 alternative fragment length(s) may be 146,582 bps INFO @ Mon, 12 Aug 2019 17:01:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.20_model.r INFO @ Mon, 12 Aug 2019 17:01:06: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:01:06: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:01:06: #1 tag size is determined as 34 bps INFO @ Mon, 12 Aug 2019 17:01:06: #1 tag size = 34 INFO @ Mon, 12 Aug 2019 17:01:06: #1 total tags in treatment: 1758736 INFO @ Mon, 12 Aug 2019 17:01:06: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 17:01:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 17:01:07: #1 tags after filtering in treatment: 1758736 INFO @ Mon, 12 Aug 2019 17:01:07: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 17:01:07: #1 finished! INFO @ Mon, 12 Aug 2019 17:01:07: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 17:01:07: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 17:01:07: #2 number of paired peaks: 584 WARNING @ Mon, 12 Aug 2019 17:01:07: Fewer paired peaks (584) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 584 pairs to build model! INFO @ Mon, 12 Aug 2019 17:01:07: start model_add_line... INFO @ Mon, 12 Aug 2019 17:01:07: start X-correlation... INFO @ Mon, 12 Aug 2019 17:01:07: end of X-cor INFO @ Mon, 12 Aug 2019 17:01:07: #2 finished! INFO @ Mon, 12 Aug 2019 17:01:07: #2 predicted fragment length is 146 bps INFO @ Mon, 12 Aug 2019 17:01:07: #2 alternative fragment length(s) may be 146,582 bps INFO @ Mon, 12 Aug 2019 17:01:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.10_model.r INFO @ Mon, 12 Aug 2019 17:01:07: #3 Call peaks... INFO @ Mon, 12 Aug 2019 17:01:07: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 12 Aug 2019 17:01:09: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:01:12: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.05_peaks.xls INFO @ Mon, 12 Aug 2019 17:01:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.05_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:01:12: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:01:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.05_summits.bed INFO @ Mon, 12 Aug 2019 17:01:12: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1210 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:01:12: #3 Call peaks for each chromosome... INFO @ Mon, 12 Aug 2019 17:01:14: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.20_peaks.xls INFO @ Mon, 12 Aug 2019 17:01:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.20_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:01:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.20_summits.bed INFO @ Mon, 12 Aug 2019 17:01:14: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (71 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 17:01:15: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.10_peaks.xls INFO @ Mon, 12 Aug 2019 17:01:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.10_peaks.narrowPeak INFO @ Mon, 12 Aug 2019 17:01:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX043862/SRX043862.10_summits.bed INFO @ Mon, 12 Aug 2019 17:01:15: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (394 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。