Job ID = 14158431 SRX = DRX184550 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 23827690 spots for DRR194095/DRR194095.sra Written 23827690 spots for DRR194095/DRR194095.sra fastq に変換しました。 bowtie でマッピング中... Your job 14159097 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:22 23827690 reads; of these: 23827690 (100.00%) were unpaired; of these: 4197042 (17.61%) aligned 0 times 14980280 (62.87%) aligned exactly 1 time 4650368 (19.52%) aligned >1 times 82.39% overall alignment rate Time searching: 00:05:22 Overall time: 00:05:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6741402 / 19630648 = 0.3434 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 17:44:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 17:44:15: #1 read tag files... INFO @ Wed, 08 Dec 2021 17:44:15: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 17:44:21: 1000000 INFO @ Wed, 08 Dec 2021 17:44:27: 2000000 INFO @ Wed, 08 Dec 2021 17:44:33: 3000000 INFO @ Wed, 08 Dec 2021 17:44:39: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 17:44:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 17:44:45: #1 read tag files... INFO @ Wed, 08 Dec 2021 17:44:45: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 17:44:45: 5000000 INFO @ Wed, 08 Dec 2021 17:44:51: 1000000 INFO @ Wed, 08 Dec 2021 17:44:51: 6000000 INFO @ Wed, 08 Dec 2021 17:44:57: 2000000 INFO @ Wed, 08 Dec 2021 17:44:58: 7000000 INFO @ Wed, 08 Dec 2021 17:45:03: 3000000 INFO @ Wed, 08 Dec 2021 17:45:04: 8000000 INFO @ Wed, 08 Dec 2021 17:45:09: 4000000 INFO @ Wed, 08 Dec 2021 17:45:10: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 17:45:15: 5000000 INFO @ Wed, 08 Dec 2021 17:45:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 17:45:15: #1 read tag files... INFO @ Wed, 08 Dec 2021 17:45:15: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 17:45:16: 10000000 INFO @ Wed, 08 Dec 2021 17:45:20: 6000000 INFO @ Wed, 08 Dec 2021 17:45:21: 1000000 INFO @ Wed, 08 Dec 2021 17:45:23: 11000000 INFO @ Wed, 08 Dec 2021 17:45:26: 7000000 INFO @ Wed, 08 Dec 2021 17:45:28: 2000000 INFO @ Wed, 08 Dec 2021 17:45:29: 12000000 INFO @ Wed, 08 Dec 2021 17:45:32: 8000000 INFO @ Wed, 08 Dec 2021 17:45:34: 3000000 INFO @ Wed, 08 Dec 2021 17:45:34: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 17:45:34: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 17:45:34: #1 total tags in treatment: 12889246 INFO @ Wed, 08 Dec 2021 17:45:34: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 17:45:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 17:45:35: #1 tags after filtering in treatment: 12889246 INFO @ Wed, 08 Dec 2021 17:45:35: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 17:45:35: #1 finished! INFO @ Wed, 08 Dec 2021 17:45:35: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 17:45:35: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 17:45:36: #2 number of paired peaks: 1663 INFO @ Wed, 08 Dec 2021 17:45:36: start model_add_line... INFO @ Wed, 08 Dec 2021 17:45:36: start X-correlation... INFO @ Wed, 08 Dec 2021 17:45:36: end of X-cor INFO @ Wed, 08 Dec 2021 17:45:36: #2 finished! INFO @ Wed, 08 Dec 2021 17:45:36: #2 predicted fragment length is 304 bps INFO @ Wed, 08 Dec 2021 17:45:36: #2 alternative fragment length(s) may be 304 bps INFO @ Wed, 08 Dec 2021 17:45:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.05_model.r INFO @ Wed, 08 Dec 2021 17:45:36: #3 Call peaks... INFO @ Wed, 08 Dec 2021 17:45:36: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 17:45:37: 9000000 INFO @ Wed, 08 Dec 2021 17:45:40: 4000000 INFO @ Wed, 08 Dec 2021 17:45:43: 10000000 INFO @ Wed, 08 Dec 2021 17:45:46: 5000000 INFO @ Wed, 08 Dec 2021 17:45:48: 11000000 INFO @ Wed, 08 Dec 2021 17:45:53: 6000000 INFO @ Wed, 08 Dec 2021 17:45:54: 12000000 INFO @ Wed, 08 Dec 2021 17:45:59: 7000000 INFO @ Wed, 08 Dec 2021 17:45:59: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 17:45:59: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 17:45:59: #1 total tags in treatment: 12889246 INFO @ Wed, 08 Dec 2021 17:45:59: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 17:45:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 17:45:59: #1 tags after filtering in treatment: 12889246 INFO @ Wed, 08 Dec 2021 17:45:59: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 17:45:59: #1 finished! INFO @ Wed, 08 Dec 2021 17:45:59: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 17:45:59: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 17:46:00: #2 number of paired peaks: 1663 INFO @ Wed, 08 Dec 2021 17:46:00: start model_add_line... INFO @ Wed, 08 Dec 2021 17:46:00: start X-correlation... INFO @ Wed, 08 Dec 2021 17:46:00: end of X-cor INFO @ Wed, 08 Dec 2021 17:46:00: #2 finished! INFO @ Wed, 08 Dec 2021 17:46:00: #2 predicted fragment length is 304 bps INFO @ Wed, 08 Dec 2021 17:46:00: #2 alternative fragment length(s) may be 304 bps INFO @ Wed, 08 Dec 2021 17:46:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.10_model.r INFO @ Wed, 08 Dec 2021 17:46:00: #3 Call peaks... INFO @ Wed, 08 Dec 2021 17:46:00: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 17:46:05: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 17:46:11: 9000000 INFO @ Wed, 08 Dec 2021 17:46:13: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 17:46:16: 10000000 INFO @ Wed, 08 Dec 2021 17:46:22: 11000000 INFO @ Wed, 08 Dec 2021 17:46:28: 12000000 INFO @ Wed, 08 Dec 2021 17:46:29: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.05_peaks.xls INFO @ Wed, 08 Dec 2021 17:46:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.05_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 17:46:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.05_summits.bed INFO @ Wed, 08 Dec 2021 17:46:29: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (7332 records, 4 fields): 9 millis CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 17:46:33: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 17:46:33: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 17:46:33: #1 total tags in treatment: 12889246 INFO @ Wed, 08 Dec 2021 17:46:33: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 17:46:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 17:46:34: #1 tags after filtering in treatment: 12889246 INFO @ Wed, 08 Dec 2021 17:46:34: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 17:46:34: #1 finished! INFO @ Wed, 08 Dec 2021 17:46:34: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 17:46:34: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 17:46:35: #2 number of paired peaks: 1663 INFO @ Wed, 08 Dec 2021 17:46:35: start model_add_line... INFO @ Wed, 08 Dec 2021 17:46:35: start X-correlation... INFO @ Wed, 08 Dec 2021 17:46:35: end of X-cor INFO @ Wed, 08 Dec 2021 17:46:35: #2 finished! INFO @ Wed, 08 Dec 2021 17:46:35: #2 predicted fragment length is 304 bps INFO @ Wed, 08 Dec 2021 17:46:35: #2 alternative fragment length(s) may be 304 bps INFO @ Wed, 08 Dec 2021 17:46:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.20_model.r INFO @ Wed, 08 Dec 2021 17:46:35: #3 Call peaks... INFO @ Wed, 08 Dec 2021 17:46:35: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 17:46:37: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 17:46:51: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.10_peaks.xls INFO @ Wed, 08 Dec 2021 17:46:51: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.10_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 17:46:51: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.10_summits.bed INFO @ Wed, 08 Dec 2021 17:46:51: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (5162 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 17:47:10: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 17:47:24: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.20_peaks.xls INFO @ Wed, 08 Dec 2021 17:47:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.20_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 17:47:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/DRX184550/DRX184550.20_summits.bed INFO @ Wed, 08 Dec 2021 17:47:25: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (3598 records, 4 fields): 19 millis CompletedMACS2peakCalling