Job ID = 14158427 SRX = DRX184548 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 32033954 spots for DRR194093/DRR194093.sra Written 32033954 spots for DRR194093/DRR194093.sra fastq に変換しました。 bowtie でマッピング中... Your job 14159099 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:25 32033954 reads; of these: 32033954 (100.00%) were unpaired; of these: 4235718 (13.22%) aligned 0 times 21775977 (67.98%) aligned exactly 1 time 6022259 (18.80%) aligned >1 times 86.78% overall alignment rate Time searching: 00:06:25 Overall time: 00:06:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8181703 / 27798236 = 0.2943 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 17:46:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 17:46:13: #1 read tag files... INFO @ Wed, 08 Dec 2021 17:46:13: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 17:46:18: 1000000 INFO @ Wed, 08 Dec 2021 17:46:23: 2000000 INFO @ Wed, 08 Dec 2021 17:46:27: 3000000 INFO @ Wed, 08 Dec 2021 17:46:32: 4000000 INFO @ Wed, 08 Dec 2021 17:46:37: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 17:46:41: 6000000 INFO @ Wed, 08 Dec 2021 17:46:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 17:46:43: #1 read tag files... INFO @ Wed, 08 Dec 2021 17:46:43: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 17:46:46: 7000000 INFO @ Wed, 08 Dec 2021 17:46:48: 1000000 INFO @ Wed, 08 Dec 2021 17:46:51: 8000000 INFO @ Wed, 08 Dec 2021 17:46:52: 2000000 INFO @ Wed, 08 Dec 2021 17:46:56: 9000000 INFO @ Wed, 08 Dec 2021 17:46:57: 3000000 INFO @ Wed, 08 Dec 2021 17:47:01: 10000000 INFO @ Wed, 08 Dec 2021 17:47:02: 4000000 INFO @ Wed, 08 Dec 2021 17:47:05: 11000000 INFO @ Wed, 08 Dec 2021 17:47:07: 5000000 INFO @ Wed, 08 Dec 2021 17:47:10: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 17:47:12: 6000000 INFO @ Wed, 08 Dec 2021 17:47:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 17:47:13: #1 read tag files... INFO @ Wed, 08 Dec 2021 17:47:13: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 17:47:15: 13000000 INFO @ Wed, 08 Dec 2021 17:47:17: 7000000 INFO @ Wed, 08 Dec 2021 17:47:18: 1000000 INFO @ Wed, 08 Dec 2021 17:47:20: 14000000 INFO @ Wed, 08 Dec 2021 17:47:22: 8000000 INFO @ Wed, 08 Dec 2021 17:47:23: 2000000 INFO @ Wed, 08 Dec 2021 17:47:25: 15000000 INFO @ Wed, 08 Dec 2021 17:47:26: 9000000 INFO @ Wed, 08 Dec 2021 17:47:28: 3000000 INFO @ Wed, 08 Dec 2021 17:47:30: 16000000 INFO @ Wed, 08 Dec 2021 17:47:31: 10000000 INFO @ Wed, 08 Dec 2021 17:47:32: 4000000 INFO @ Wed, 08 Dec 2021 17:47:34: 17000000 INFO @ Wed, 08 Dec 2021 17:47:36: 11000000 INFO @ Wed, 08 Dec 2021 17:47:37: 5000000 INFO @ Wed, 08 Dec 2021 17:47:39: 18000000 INFO @ Wed, 08 Dec 2021 17:47:41: 12000000 INFO @ Wed, 08 Dec 2021 17:47:42: 6000000 INFO @ Wed, 08 Dec 2021 17:47:44: 19000000 INFO @ Wed, 08 Dec 2021 17:47:46: 13000000 INFO @ Wed, 08 Dec 2021 17:47:47: 7000000 INFO @ Wed, 08 Dec 2021 17:47:47: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 17:47:47: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 17:47:47: #1 total tags in treatment: 19616533 INFO @ Wed, 08 Dec 2021 17:47:47: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 17:47:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 17:47:48: #1 tags after filtering in treatment: 19616533 INFO @ Wed, 08 Dec 2021 17:47:48: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 17:47:48: #1 finished! INFO @ Wed, 08 Dec 2021 17:47:48: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 17:47:48: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 17:47:49: #2 number of paired peaks: 344 WARNING @ Wed, 08 Dec 2021 17:47:49: Fewer paired peaks (344) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 344 pairs to build model! INFO @ Wed, 08 Dec 2021 17:47:49: start model_add_line... INFO @ Wed, 08 Dec 2021 17:47:49: start X-correlation... INFO @ Wed, 08 Dec 2021 17:47:49: end of X-cor INFO @ Wed, 08 Dec 2021 17:47:49: #2 finished! INFO @ Wed, 08 Dec 2021 17:47:49: #2 predicted fragment length is 200 bps INFO @ Wed, 08 Dec 2021 17:47:49: #2 alternative fragment length(s) may be 3,178,200 bps INFO @ Wed, 08 Dec 2021 17:47:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.05_model.r INFO @ Wed, 08 Dec 2021 17:47:49: #3 Call peaks... INFO @ Wed, 08 Dec 2021 17:47:49: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 17:47:51: 14000000 INFO @ Wed, 08 Dec 2021 17:47:52: 8000000 INFO @ Wed, 08 Dec 2021 17:47:56: 15000000 INFO @ Wed, 08 Dec 2021 17:47:57: 9000000 INFO @ Wed, 08 Dec 2021 17:48:00: 16000000 INFO @ Wed, 08 Dec 2021 17:48:02: 10000000 INFO @ Wed, 08 Dec 2021 17:48:05: 17000000 INFO @ Wed, 08 Dec 2021 17:48:06: 11000000 INFO @ Wed, 08 Dec 2021 17:48:10: 18000000 INFO @ Wed, 08 Dec 2021 17:48:11: 12000000 INFO @ Wed, 08 Dec 2021 17:48:15: 19000000 INFO @ Wed, 08 Dec 2021 17:48:16: 13000000 INFO @ Wed, 08 Dec 2021 17:48:18: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 17:48:18: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 17:48:18: #1 total tags in treatment: 19616533 INFO @ Wed, 08 Dec 2021 17:48:18: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 17:48:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 17:48:18: #1 tags after filtering in treatment: 19616533 INFO @ Wed, 08 Dec 2021 17:48:18: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 17:48:18: #1 finished! INFO @ Wed, 08 Dec 2021 17:48:18: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 17:48:18: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 17:48:20: #2 number of paired peaks: 344 WARNING @ Wed, 08 Dec 2021 17:48:20: Fewer paired peaks (344) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 344 pairs to build model! INFO @ Wed, 08 Dec 2021 17:48:20: start model_add_line... INFO @ Wed, 08 Dec 2021 17:48:20: start X-correlation... INFO @ Wed, 08 Dec 2021 17:48:20: end of X-cor INFO @ Wed, 08 Dec 2021 17:48:20: #2 finished! INFO @ Wed, 08 Dec 2021 17:48:20: #2 predicted fragment length is 200 bps INFO @ Wed, 08 Dec 2021 17:48:20: #2 alternative fragment length(s) may be 3,178,200 bps INFO @ Wed, 08 Dec 2021 17:48:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.10_model.r INFO @ Wed, 08 Dec 2021 17:48:20: #3 Call peaks... INFO @ Wed, 08 Dec 2021 17:48:20: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 17:48:21: 14000000 INFO @ Wed, 08 Dec 2021 17:48:26: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 17:48:30: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 17:48:30: 16000000 INFO @ Wed, 08 Dec 2021 17:48:35: 17000000 INFO @ Wed, 08 Dec 2021 17:48:39: 18000000 INFO @ Wed, 08 Dec 2021 17:48:44: 19000000 INFO @ Wed, 08 Dec 2021 17:48:47: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 17:48:47: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 17:48:47: #1 total tags in treatment: 19616533 INFO @ Wed, 08 Dec 2021 17:48:47: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 17:48:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 17:48:47: #1 tags after filtering in treatment: 19616533 INFO @ Wed, 08 Dec 2021 17:48:47: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 17:48:47: #1 finished! INFO @ Wed, 08 Dec 2021 17:48:47: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 17:48:47: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 17:48:49: #2 number of paired peaks: 344 WARNING @ Wed, 08 Dec 2021 17:48:49: Fewer paired peaks (344) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 344 pairs to build model! INFO @ Wed, 08 Dec 2021 17:48:49: start model_add_line... INFO @ Wed, 08 Dec 2021 17:48:49: start X-correlation... INFO @ Wed, 08 Dec 2021 17:48:49: end of X-cor INFO @ Wed, 08 Dec 2021 17:48:49: #2 finished! INFO @ Wed, 08 Dec 2021 17:48:49: #2 predicted fragment length is 200 bps INFO @ Wed, 08 Dec 2021 17:48:49: #2 alternative fragment length(s) may be 3,178,200 bps INFO @ Wed, 08 Dec 2021 17:48:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.20_model.r INFO @ Wed, 08 Dec 2021 17:48:49: #3 Call peaks... INFO @ Wed, 08 Dec 2021 17:48:49: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 17:48:50: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.05_peaks.xls INFO @ Wed, 08 Dec 2021 17:48:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.05_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 17:48:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.05_summits.bed INFO @ Wed, 08 Dec 2021 17:48:50: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (15698 records, 4 fields): 16 millis CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 17:49:00: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 17:49:20: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.10_peaks.xls INFO @ Wed, 08 Dec 2021 17:49:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.10_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 17:49:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.10_summits.bed INFO @ Wed, 08 Dec 2021 17:49:20: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (11945 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 17:49:28: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 17:49:47: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.20_peaks.xls INFO @ Wed, 08 Dec 2021 17:49:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.20_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 17:49:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/DRX184548/DRX184548.20_summits.bed INFO @ Wed, 08 Dec 2021 17:49:48: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (6560 records, 4 fields): 7 millis CompletedMACS2peakCalling